FastQCFastQC Report
Sat 18 Jun 2016
SRR3550166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359084
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC70680.5200561554694191No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC49360.36318579278396335No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT37620.2768040827498521No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC27410.20167995502853392No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC18850.1386963572523847No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.11838856170773844No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG13860.1019804515394192No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG253.891182E-545.0000041
TACGACA253.891182E-545.0000041
AGTTACG351.2121382E-745.0000041
GTAAGCG253.891182E-545.0000041
CGTAACG207.033742E-445.02
TTACGCG207.033742E-445.01
CGATCTA207.033742E-445.010
TCGACGT207.033742E-445.026
GTGCGAT207.033742E-445.011
ATACCGA207.033742E-445.015
ATTTCGC207.033742E-445.045
CTAAGCG302.165796E-644.9999961
TAGCCGT302.165796E-644.99999644
TACACGA302.165796E-644.99999637
CGATATA750.042.010
CGTACAT700.041.78571735
CGTTAGG1950.040.3846172
TCACGAC451.9286745E-840.025
TAGGGTA4100.039.5121965
TACACGC5650.039.42477835