##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1359084
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98327108552525	33.0	31.0	34.0	30.0	34.0
2	32.13507185722148	33.0	31.0	34.0	30.0	34.0
3	32.193591418926275	34.0	31.0	34.0	30.0	34.0
4	35.768128386472064	37.0	35.0	37.0	35.0	37.0
5	35.782593276059465	37.0	35.0	37.0	35.0	37.0
6	35.780699353388016	37.0	35.0	37.0	35.0	37.0
7	35.985079656592234	37.0	35.0	37.0	35.0	37.0
8	35.93903982388138	37.0	35.0	37.0	35.0	37.0
9	37.49810902048733	39.0	37.0	39.0	35.0	39.0
10	37.20580479205112	39.0	37.0	39.0	34.0	39.0
11	37.164627057635876	39.0	37.0	39.0	34.0	39.0
12	37.01948886161562	39.0	37.0	39.0	33.0	39.0
13	36.927111937157676	39.0	37.0	39.0	33.0	39.0
14	37.99844159742886	40.0	37.0	41.0	33.0	41.0
15	38.07196317519741	40.0	37.0	41.0	33.0	41.0
16	38.14740222090761	40.0	37.0	41.0	33.0	41.0
17	38.146735595445165	40.0	37.0	41.0	33.0	41.0
18	38.056736007487395	40.0	37.0	41.0	33.0	41.0
19	38.008988406897586	40.0	37.0	41.0	33.0	41.0
20	37.89268286581256	40.0	36.0	41.0	33.0	41.0
21	37.780008446865686	40.0	36.0	41.0	33.0	41.0
22	37.75068722757386	40.0	36.0	41.0	33.0	41.0
23	37.74425127512354	40.0	36.0	41.0	33.0	41.0
24	37.786985940530535	40.0	36.0	41.0	33.0	41.0
25	37.71994961312178	40.0	36.0	41.0	33.0	41.0
26	37.67062153627002	40.0	36.0	41.0	33.0	41.0
27	37.58129666746132	40.0	36.0	41.0	33.0	41.0
28	37.502060946931906	39.0	36.0	41.0	33.0	41.0
29	37.46801595780688	39.0	36.0	41.0	32.0	41.0
30	37.359016808379764	39.0	36.0	41.0	32.0	41.0
31	37.30444402259169	39.0	35.0	41.0	32.0	41.0
32	37.22200467373614	39.0	35.0	41.0	32.0	41.0
33	37.16183473574849	39.0	35.0	41.0	32.0	41.0
34	37.06709151163578	39.0	35.0	41.0	31.0	41.0
35	37.01230019630869	39.0	35.0	41.0	31.0	41.0
36	36.92705896029973	39.0	35.0	41.0	31.0	41.0
37	36.88747568215062	39.0	35.0	41.0	31.0	41.0
38	36.77379470290284	39.0	35.0	41.0	31.0	41.0
39	36.7246719113756	39.0	35.0	41.0	31.0	41.0
40	36.58042475667435	39.0	35.0	40.0	30.0	41.0
41	36.490132324418504	39.0	35.0	40.0	30.0	41.0
42	36.445064469892955	39.0	35.0	40.0	30.0	41.0
43	36.38072407592173	39.0	35.0	40.0	30.0	41.0
44	36.2736710902343	38.0	35.0	40.0	30.0	41.0
45	36.17065096785776	38.0	35.0	40.0	30.0	41.0
46	36.12849021841181	38.0	35.0	40.0	30.0	41.0
47	36.054647100547136	38.0	35.0	40.0	30.0	41.0
48	36.0514780543366	38.0	35.0	40.0	30.0	41.0
49	36.003533997898586	38.0	35.0	40.0	30.0	41.0
50	35.891422458067346	38.0	35.0	40.0	30.0	41.0
51	34.59226949916267	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	21.0
9	50.0
10	50.0
11	52.0
12	32.0
13	27.0
14	29.0
15	43.0
16	90.0
17	179.0
18	334.0
19	572.0
20	1040.0
21	1571.0
22	2414.0
23	3660.0
24	5463.0
25	7344.0
26	9715.0
27	12260.0
28	15251.0
29	18494.0
30	23487.0
31	29793.0
32	38462.0
33	52757.0
34	86535.0
35	109648.0
36	113359.0
37	163847.0
38	271125.0
39	391255.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45263721741997	21.462396731916495	25.317051778992322	10.767914271671215
2	31.30255377886871	24.829959001798272	25.275773977178744	18.591713242154277
3	31.444266873865047	25.12692372215404	26.02782462305494	17.400984780925977
4	29.033893416448137	26.25739100747268	25.187332056002425	19.521383520076757
5	25.719234425539554	30.555874397756135	23.612153479843776	20.112737696860535
6	27.524126544054674	33.370785028739945	23.410032050999057	15.695056376206326
7	79.25146642885944	6.482380779995939	8.823148532393878	5.443004258750747
8	78.2513074982856	5.567131980068929	9.505299157373642	6.676261364271818
9	71.2104623408119	8.315968696563274	12.07717845254598	8.396390510078847
10	38.49269066518332	25.571782170932778	19.637196817856733	16.298330346027175
11	29.646806231255756	25.454423714796143	23.752027100605996	21.146742953342105
12	27.069629250289168	21.204870339140186	30.843936062818784	20.88156434775187
13	24.91141092088495	23.5196647153524	31.1539242607521	20.41500010301056
14	20.784734424067974	27.46570484237913	28.903732219642052	22.845828513910842
15	19.2214756409464	25.480029196135046	34.08766492725983	21.210830235658722
16	21.060361243307995	25.133987303213047	31.132439201697615	22.673212251781347
17	21.046528397067437	25.443534027330173	28.15653778574393	25.353399789858464
18	22.190092738932986	24.991464839553696	29.817215124304308	23.001227297209002
19	23.12881323008732	26.5305161417543	27.27020552077723	23.070465107381146
20	25.37238316395455	25.515714996276905	27.70137828125414	21.41052355851441
21	24.24655135370588	26.429565795785987	27.852141589482326	21.47174126102581
22	21.967295619696795	25.437574130811637	25.65316051105009	26.94196973844148
23	21.682471429286196	26.995903122985776	27.15615811826201	24.165467329466022
24	23.226599680372956	23.737311306733062	28.67791836266191	24.35817065023207
25	21.819181154365737	26.678557028115996	26.017155672497065	25.48510614502121
26	21.698070170791503	26.477539283811748	26.858384029243226	24.966006516153527
27	23.303857598205852	26.02260051623005	27.698361543510185	22.975180342053914
28	19.765077066612513	25.617842605755055	29.26448990643698	25.352590421195455
29	21.223117923542624	24.517469118906558	28.448572715152267	25.81084024239856
30	22.34740457543463	25.757642647547907	28.036604065679533	23.858348711337932
31	22.0393294307048	25.78074644392841	26.126714757881043	26.053209367485746
32	21.27933225613722	25.88640584393606	27.339958383734924	25.494303516191785
33	21.557902234151825	26.411097474475454	27.912697081269442	24.118303210103274
34	19.381877794161362	24.121908579602145	29.759087738506228	26.73712588773027
35	19.973084813006405	22.978417816705957	29.02903720447007	28.019460165817566
36	20.314711967766524	25.495333621762896	29.659682550894573	24.530271859576008
37	19.539189630663007	25.173572788731235	31.419691498097247	23.86754608250851
38	19.558982373422097	26.13223318058339	28.808447454314816	25.50033699167969
39	21.444664200299613	23.581838944465535	29.988139070138413	24.985357785096433
40	22.167136100491213	23.010424668379585	29.527681879854374	25.29475735127483
41	18.597525980734083	24.42549540720073	28.312893095643833	28.664085516421355
42	21.053518399157078	23.05045162771396	30.32527790776729	25.570752065361667
43	22.393612168195638	22.143443672355794	31.38040032845652	24.08254383099205
44	21.48785505531667	23.38185130573239	29.168690088324194	25.961603550626744
45	21.118341471167344	22.31775225078067	28.690279629515175	27.87362664853681
46	21.736257655891762	23.770936895732714	28.295749195781866	26.197056252593658
47	18.47074941651877	23.54269493276354	31.4505946652304	26.535960985487282
48	19.847706249209025	22.42569259883863	30.665580641078844	27.0610205108735
49	20.603583001492183	21.241659823822516	32.248337851082056	25.906419323603252
50	19.52344373121897	21.47762757857498	31.46972519726522	27.529203492940834
51	19.436105494583117	21.771501982217433	27.83234884672323	30.96004367647621
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	402.0
1	1068.0
2	1734.0
3	2551.0
4	3368.0
5	2488.0
6	1608.0
7	1583.0
8	1558.0
9	1705.5
10	1853.0
11	1991.0
12	2129.0
13	2301.0
14	2473.0
15	2309.0
16	2145.0
17	2458.0
18	2771.0
19	2883.5
20	2996.0
21	3305.0
22	3614.0
23	4123.5
24	4633.0
25	5744.5
26	7614.0
27	8372.0
28	10184.5
29	11997.0
30	15129.5
31	18262.0
32	20791.0
33	23320.0
34	26010.0
35	28700.0
36	31802.0
37	34904.0
38	38948.5
39	42993.0
40	51420.0
41	59847.0
42	70326.5
43	80806.0
44	100490.5
45	120175.0
46	139163.5
47	158152.0
48	157045.0
49	155938.0
50	131838.5
51	107739.0
52	94794.0
53	81849.0
54	73157.5
55	64466.0
56	61529.5
57	58593.0
58	57209.5
59	55826.0
60	55482.5
61	55139.0
62	50110.0
63	45081.0
64	39914.5
65	34748.0
66	29863.0
67	24978.0
68	21170.5
69	17363.0
70	14654.5
71	11946.0
72	10226.5
73	8507.0
74	6568.5
75	3634.0
76	2638.0
77	2141.5
78	1645.0
79	1578.0
80	1511.0
81	976.5
82	442.0
83	310.5
84	179.0
85	149.5
86	120.0
87	83.0
88	46.0
89	31.0
90	16.0
91	11.5
92	7.0
93	6.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1359084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.456760633670797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.82299782496783	15.909078254382456
2	12.529181884207347	5.877880407871523
3	5.497601296658431	3.86867753025225
4	2.953309382512246	2.7710028505105537
5	1.8122276553114893	2.1254495162180036
6	1.2027579377113984	1.692768302708628
7	0.8842437915525102	1.4519046472179709
8	0.6419853487795305	1.2047117325316083
9	0.46986817617311155	0.9919426804084924
>10	4.218069758397589	22.59913561298165
>50	1.6080225586749664	27.135938035731034
>100	0.34926592411349233	11.260090364341972
>500	0.007613426138240377	1.19533773189963
>1k	0.0025378087127467925	1.390145636662655
>5k	3.1722608909334906E-4	0.5259366962815354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	7068	0.5200561554694191	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	4936	0.36318579278396335	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	3762	0.2768040827498521	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2741	0.20167995502853392	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1885	0.1386963572523847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1609	0.11838856170773844	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	1386	0.1019804515394192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2073690809398095E-4	0.0	0.0	0.30542630183270497	0.0
2	2.2073690809398095E-4	0.0	0.0	1.630877855967696	0.0
3	2.2073690809398095E-4	0.0	0.0	2.2846269987727026	0.0
4	2.2073690809398095E-4	0.0	0.0	3.0670657589965007	0.0
5	2.2073690809398095E-4	0.0	0.0	5.232641985337183	0.0
6	2.2073690809398095E-4	0.0	0.0	6.365169481798034	0.0
7	2.2073690809398095E-4	0.0	0.0	7.5744398433062265	0.0
8	2.2073690809398095E-4	0.0	0.0	9.572108861556755	0.0
9	2.2073690809398095E-4	0.0	0.0	10.322172875260101	0.0
10	2.2073690809398095E-4	0.0	0.0	11.917806404902125	0.0
11	2.2073690809398095E-4	0.0	0.0	13.915769739030111	0.0
12	2.2073690809398095E-4	0.0	0.0	15.478071995549945	0.0
13	2.2073690809398095E-4	0.0	0.0	16.130938190722574	0.0
14	2.2073690809398095E-4	0.0	0.0	16.377722053971645	0.0
15	2.2073690809398095E-4	0.0	0.0	16.837296296623315	0.0
16	2.2073690809398095E-4	0.0	0.0	17.808317955328736	0.0
17	2.2073690809398095E-4	0.0	0.0	18.967848933546417	0.0
18	2.2073690809398095E-4	0.0	0.0	20.253420686285764	0.0
19	2.2073690809398095E-4	0.0	0.0	20.960588160849515	0.0
20	2.2073690809398095E-4	0.0	0.0	21.65598300031492	0.0
21	2.2073690809398095E-4	0.0	0.0	22.47999387822975	0.0
22	2.2073690809398095E-4	0.0	0.0	23.361984984003932	0.0
23	2.2073690809398095E-4	0.0	0.0	24.19843070774139	0.0
24	2.2073690809398095E-4	0.0	0.0	24.859979221299053	0.0
25	2.2073690809398095E-4	0.0	0.0	25.432497181925473	0.0
26	2.9431587745864125E-4	0.0	0.0	25.94100143920464	0.0
27	2.9431587745864125E-4	0.0	0.0	26.47672991514873	0.0
28	2.9431587745864125E-4	0.0	0.0	27.010545337889344	0.0
29	2.9431587745864125E-4	0.0	0.0	27.570775610631866	0.0
30	3.678948468233016E-4	0.0	0.0	28.22665854354845	0.0
31	4.414738161879619E-4	0.0	0.0	28.797999240665035	0.0
32	4.414738161879619E-4	0.0	0.0	29.382142678451075	0.0
33	4.414738161879619E-4	0.0	0.0	29.91014536261188	0.0
34	5.150527855526222E-4	0.0	0.0	30.424168042593394	0.0
35	5.150527855526222E-4	0.0	0.0	30.991609054333654	0.0
36	5.886317549172825E-4	0.0	0.0	31.54999985284206	0.0
37	5.886317549172825E-4	0.0	0.0	32.10699265093254	0.0
38	5.886317549172825E-4	0.0	0.0	32.642426810999176	0.0
39	5.886317549172825E-4	0.0	0.0	33.18345297273752	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	25	3.891182E-5	45.000004	1
TACGACA	25	3.891182E-5	45.000004	1
AGTTACG	35	1.2121382E-7	45.000004	1
GTAAGCG	25	3.891182E-5	45.000004	1
CGTAACG	20	7.033742E-4	45.0	2
TTACGCG	20	7.033742E-4	45.0	1
CGATCTA	20	7.033742E-4	45.0	10
TCGACGT	20	7.033742E-4	45.0	26
GTGCGAT	20	7.033742E-4	45.0	11
ATACCGA	20	7.033742E-4	45.0	15
ATTTCGC	20	7.033742E-4	45.0	45
CTAAGCG	30	2.165796E-6	44.999996	1
TAGCCGT	30	2.165796E-6	44.999996	44
TACACGA	30	2.165796E-6	44.999996	37
CGATATA	75	0.0	42.0	10
CGTACAT	70	0.0	41.785717	35
CGTTAGG	195	0.0	40.384617	2
TCACGAC	45	1.9286745E-8	40.0	25
TAGGGTA	410	0.0	39.512196	5
TACACGC	565	0.0	39.424778	35
>>END_MODULE