FastQCFastQC Report
Sat 18 Jun 2016
SRR3550165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245667
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC58570.4701898661520294TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC53160.4267593185016541TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT35720.286754004079742TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC23250.18664699313701014TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC18960.15220761246785858No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG17670.1418517147841277No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC17550.1408883754647109No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC16340.13117470399392453TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.1274016249928753No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG13230.10620815996570511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC302.1656488E-645.00000440
CTTCGAA207.033514E-445.019
TAATACG406.8157533E-945.01
CGAACCG207.033514E-445.031
ACTGCGT207.033514E-445.023
GTCGACG406.8157533E-945.02
ACGCATA207.033514E-445.042
CGTGCCG207.033514E-445.014
CGTGCCA207.033514E-445.024
CGTGACG406.8157533E-945.018
CGCATCG253.8909944E-545.021
ACTCTCG207.033514E-445.012
CCGGATA253.8909944E-545.027
GCACGAC351.212029E-745.09
ATTCGAT207.033514E-445.014
TCCTACG207.033514E-445.021
CCGTATC207.033514E-445.042
GTTAACG207.033514E-445.01
TACGATC207.033514E-445.011
CATACGT207.033514E-445.011
Produced by FastQC (version 0.11.5)