##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1245667
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01905083782423	33.0	31.0	34.0	30.0	34.0
2	32.16639198116351	34.0	31.0	34.0	30.0	34.0
3	32.22951238171999	34.0	31.0	34.0	30.0	34.0
4	35.79502467352832	37.0	35.0	37.0	35.0	37.0
5	35.807743963675684	37.0	35.0	37.0	35.0	37.0
6	35.8130583855878	37.0	35.0	37.0	35.0	37.0
7	36.00558576248709	37.0	35.0	37.0	35.0	37.0
8	35.95943618960766	37.0	35.0	37.0	35.0	37.0
9	37.56686738911764	39.0	37.0	39.0	35.0	39.0
10	37.298053171513736	39.0	37.0	39.0	34.0	39.0
11	37.21194829757873	39.0	37.0	39.0	34.0	39.0
12	36.99730505825393	39.0	37.0	39.0	33.0	39.0
13	36.88613971470706	39.0	37.0	39.0	33.0	39.0
14	37.98107279072176	40.0	37.0	41.0	33.0	41.0
15	38.05451617486857	40.0	37.0	41.0	33.0	41.0
16	38.13993065562466	40.0	37.0	41.0	33.0	41.0
17	38.10641848905045	40.0	37.0	41.0	33.0	41.0
18	38.02055444994529	40.0	37.0	41.0	33.0	41.0
19	37.96787423926298	40.0	37.0	41.0	33.0	41.0
20	37.85007710728469	40.0	36.0	41.0	33.0	41.0
21	37.74054944058083	40.0	36.0	41.0	33.0	41.0
22	37.70154142318934	39.0	36.0	41.0	33.0	41.0
23	37.72907446372104	40.0	36.0	41.0	33.0	41.0
24	37.77774076057245	40.0	36.0	41.0	33.0	41.0
25	37.72580392673162	40.0	36.0	41.0	33.0	41.0
26	37.6695015602083	40.0	36.0	41.0	33.0	41.0
27	37.576666958344404	39.0	36.0	41.0	33.0	41.0
28	37.50141410184263	39.0	36.0	41.0	33.0	41.0
29	37.43108631761137	39.0	35.0	41.0	33.0	41.0
30	37.30563384917478	39.0	35.0	41.0	32.0	41.0
31	37.22440347219602	39.0	35.0	41.0	32.0	41.0
32	37.06721940936061	39.0	35.0	41.0	32.0	41.0
33	37.000288199013056	39.0	35.0	41.0	31.0	41.0
34	36.878657779326254	39.0	35.0	41.0	31.0	41.0
35	36.75428425092741	39.0	35.0	41.0	31.0	41.0
36	36.67643118104598	39.0	35.0	41.0	31.0	41.0
37	36.62045233597743	39.0	35.0	40.0	31.0	41.0
38	36.47338494156143	39.0	35.0	40.0	30.0	41.0
39	36.4848221876312	39.0	35.0	40.0	30.0	41.0
40	36.34368896342281	38.0	35.0	40.0	30.0	41.0
41	36.26042513769731	38.0	35.0	40.0	30.0	41.0
42	36.16622259399984	38.0	35.0	40.0	30.0	41.0
43	36.10503449156155	38.0	35.0	40.0	30.0	41.0
44	36.027345992147175	38.0	35.0	40.0	30.0	41.0
45	35.92840863569477	38.0	35.0	40.0	30.0	41.0
46	35.896475542821634	38.0	35.0	40.0	30.0	41.0
47	35.78913385359009	38.0	35.0	40.0	29.0	41.0
48	35.73078037709918	38.0	35.0	40.0	29.0	41.0
49	35.69932895388575	38.0	34.0	40.0	29.0	41.0
50	35.57561691848624	37.0	34.0	40.0	29.0	41.0
51	34.23639704672276	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	13.0
9	45.0
10	60.0
11	39.0
12	23.0
13	17.0
14	30.0
15	50.0
16	81.0
17	158.0
18	316.0
19	615.0
20	1022.0
21	1582.0
22	2436.0
23	3477.0
24	5082.0
25	6989.0
26	9445.0
27	11413.0
28	14168.0
29	17560.0
30	22138.0
31	27764.0
32	35964.0
33	50195.0
34	85014.0
35	109277.0
36	104001.0
37	151100.0
38	250725.0
39	334783.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96265936241387	21.78768483069713	25.083589755528564	11.166066051360437
2	31.08214314098391	25.081663076889733	25.825601866309373	18.01059191581699
3	32.23710670668806	23.749685911242732	26.729053591369123	17.28415379070008
4	28.939596216324265	26.469112531679816	26.134833787842176	18.456457464153743
5	25.16451025835958	30.689100698661843	24.62190938669805	19.52447965628053
6	27.3611647414598	34.083828182010116	22.860603997697616	15.694403078832464
7	78.84009129245617	6.320870666076889	9.409898472063562	5.429139569403381
8	78.64951066376487	5.599168959280449	9.249422197104042	6.501898179850635
9	72.43083424382279	7.709444016739626	11.639145935470715	8.22057580396687
10	40.18899111881426	24.335797608831253	19.06753570577048	16.407675566584007
11	30.730444011120145	23.753057598860693	24.197799251324792	21.31869913869437
12	26.22811714527237	21.657634022575856	30.904567593104737	21.209681239047033
13	24.398494942869963	23.76726685382209	32.153456742452036	19.68078146085591
14	20.628386238055597	27.010268394362217	29.23124719527771	23.130098172304475
15	19.535638336730443	24.45276305786378	35.28077728638553	20.73082131902025
16	21.8537538523538	23.795524807191647	30.60103542921182	23.749685911242732
17	21.088139928247276	24.71960804934224	28.40606678992058	25.786185232489906
18	22.883724141363622	23.81711966360191	30.407083112902566	22.8920730821319
19	22.911018755413767	26.339061723558544	27.307298017849075	23.442621503178618
20	25.32892016887338	25.989369550610235	26.714924614684342	21.966785665832038
21	24.09865557970148	26.9519863655375	27.44039940048183	21.50895865427919
22	22.337029077594575	25.08704172142314	26.058328590225155	26.517600610757132
23	21.926004301310062	25.900742333223885	27.632264481599016	24.540988883867037
24	23.5962741246256	23.070290856224016	28.829534699080895	24.503900320069487
25	22.1812089426789	25.581555905390445	26.39790570031959	25.839329451611064
26	22.335262955508977	25.707673077957434	27.306093843699802	24.65097012283379
27	22.624987255823587	26.948373843089684	26.8413629003578	23.58527600072893
28	19.980299710917926	26.338499775622214	29.297557051764233	24.383643461695623
29	23.37719470773489	25.73448602234787	26.682652747483875	24.205666522433365
30	23.95624191698102	27.059478977929093	26.343316472219303	22.640962632870583
31	25.05693736769137	26.809974094200133	25.090172574211245	23.042915963897254
32	24.600394808564406	28.398038962258777	24.59485560747776	22.40671062169906
33	24.70050181950714	28.16523196006637	25.114737726856372	22.01952849357011
34	24.360443039753	25.976926417734436	28.104140191560024	21.558490350952543
35	23.612811449608927	26.63023103285228	26.726243851687492	23.03071366585131
36	25.215727798841908	28.239489365938088	25.939757575660266	20.605025259559735
37	23.96507252740901	27.656428242861054	28.659746144033676	19.718753085696257
38	22.547358162333914	28.9606291247982	26.78067252323454	21.711340189633347
39	25.731435447836382	25.285730456052864	26.833254794419375	22.149579301691382
40	25.24085489942336	24.468738434910776	28.712970641431458	21.577436024234405
41	23.5705047978312	24.166972393103453	26.863198591597914	25.399324217467427
42	24.03692158498218	23.768631584524595	28.817653514141423	23.3767933163518
43	24.62937526642353	24.12482629787897	28.12388864760807	23.121909788089432
44	22.470692408163657	25.008047897230963	27.38845935550994	25.13280033909544
45	22.811232857577508	24.59116280675333	26.637456077747906	25.960148257921254
46	24.73261313015437	24.37384951194822	27.60537125893196	23.288166098965455
47	22.336547407934866	23.847304295610304	31.04280678544105	22.77334151101378
48	22.43866137579305	23.17810458172208	29.738204512120813	24.645029530364056
49	23.734673873515153	22.845752516523277	29.96394702597083	23.455626583990746
50	22.106469867147478	22.269515046958777	30.266676407097563	25.357338678796175
51	21.802375755318234	22.194615414874118	27.344948529582947	28.658060300224697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	570.0
1	1173.5
2	1777.0
3	2588.0
4	3399.0
5	2480.5
6	1562.0
7	1567.0
8	1572.0
9	1655.5
10	1739.0
11	1826.0
12	1913.0
13	2054.5
14	2196.0
15	2166.5
16	2137.0
17	2390.5
18	2644.0
19	2804.5
20	2965.0
21	3081.5
22	3198.0
23	3906.5
24	4615.0
25	5216.5
26	6579.0
27	7340.0
28	9781.0
29	12222.0
30	12652.0
31	13082.0
32	16586.0
33	20090.0
34	23047.5
35	26005.0
36	29015.0
37	32025.0
38	35505.5
39	38986.0
40	47438.0
41	55890.0
42	66187.5
43	76485.0
44	95302.0
45	114119.0
46	128155.0
47	142191.0
48	137582.5
49	132974.0
50	112912.5
51	92851.0
52	82911.5
53	72972.0
54	67601.5
55	62231.0
56	57528.0
57	52825.0
58	52007.0
59	51189.0
60	50776.0
61	50363.0
62	47637.0
63	44911.0
64	38845.0
65	32779.0
66	28846.5
67	24914.0
68	21449.5
69	17985.0
70	14952.0
71	11919.0
72	10557.5
73	9196.0
74	7593.0
75	4957.5
76	3925.0
77	2753.5
78	1582.0
79	1502.0
80	1422.0
81	931.0
82	440.0
83	494.0
84	548.0
85	303.0
86	58.0
87	44.0
88	30.0
89	20.0
90	10.0
91	9.5
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1245667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.94768181193581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.29959039690007	14.770699961047736
2	12.563750223794345	5.514903845529512
3	5.654809863433952	3.723299027689338
4	3.1471998681530193	2.762949652191552
5	1.9369808252820708	2.125611941455586
6	1.30232532241837	1.7149813075239058
7	0.8836601580528582	1.3576004385180327
8	0.6492882382430455	1.1400297325752586
9	0.49818292055963465	0.9840564202125378
>10	3.9044552662955425	18.840348431918976
>50	1.4424262941296233	23.11684961951257
>100	0.7080866714135416	20.82183216611834
>500	0.004437096635489077	0.6682975979170541
>1k	0.004067338582531654	1.551813802959309
>5k	7.395161059148462E-4	0.9067260548302666
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	5857	0.4701898661520294	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	5316	0.4267593185016541	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3572	0.286754004079742	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2325	0.18664699313701014	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	1896	0.15220761246785858	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	1767	0.1418517147841277	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1755	0.1408883754647109	No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1634	0.13117470399392453	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1587	0.1274016249928753	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	1323	0.10620815996570511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.408348298542066E-4	0.0	0.0	0.3141288964065035	0.0
2	2.408348298542066E-4	0.0	0.0	1.6438582703081963	0.0
3	2.408348298542066E-4	0.0	0.0	2.30591321757741	0.0
4	2.408348298542066E-4	0.0	0.0	3.0314682816515166	0.0
5	2.408348298542066E-4	0.0	0.0	5.088037172053205	0.0
6	2.408348298542066E-4	0.0	0.0	6.162240791479585	0.0
7	2.408348298542066E-4	0.0	0.0	7.3339022387203	0.0
8	2.408348298542066E-4	0.0	0.0	9.21867561715932	0.0
9	2.408348298542066E-4	0.0	0.0	9.900719855306434	0.0
10	2.408348298542066E-4	0.0	0.0	11.44816391539633	0.0
11	2.408348298542066E-4	0.0	0.0	13.342249573923047	0.0
12	2.408348298542066E-4	0.0	0.0	14.964994657480691	0.0
13	2.408348298542066E-4	0.0	0.0	15.537298491490905	0.0
14	2.408348298542066E-4	0.0	0.0	15.768339371597706	0.0
15	2.408348298542066E-4	0.0	0.0	16.254344058243497	0.0
16	3.211131064722755E-4	0.0	0.0	17.209655549998516	0.0
17	3.211131064722755E-4	0.0	0.0	18.349446521421857	0.0
18	3.211131064722755E-4	0.0	0.0	19.61559550024204	0.0
19	3.211131064722755E-4	0.0	0.0	20.284233266193933	0.0
20	4.0139138309034435E-4	0.0	0.0	20.960658024977782	0.0
21	4.0139138309034435E-4	0.0	0.0	21.7688997139685	0.0
22	4.0139138309034435E-4	0.0	0.0	22.57657945502289	0.0
23	4.0139138309034435E-4	0.0	0.0	23.370290775945737	0.0
24	4.0139138309034435E-4	0.0	0.0	24.035958245662766	0.0
25	4.0139138309034435E-4	0.0	0.0	24.622631891187613	0.0
26	4.0139138309034435E-4	0.0	0.0	25.127020303178938	0.0
27	4.816696597084132E-4	0.0	0.0	25.63164955000012	0.0
28	4.816696597084132E-4	0.0	0.0	26.133549335416287	0.0
29	4.816696597084132E-4	0.0	0.0	26.66306484798907	0.0
30	4.816696597084132E-4	0.0	0.0	27.28080618656511	0.0
31	4.816696597084132E-4	0.0	0.0	27.846206088786168	0.0
32	4.816696597084132E-4	0.0	0.0	28.382143863488395	0.0
33	4.816696597084132E-4	0.0	0.0	28.913586054700012	0.0
34	4.816696597084132E-4	0.0	0.0	29.433548452355243	0.0
35	4.816696597084132E-4	0.0	0.0	29.988833291722425	0.0
36	4.816696597084132E-4	0.0	0.0	30.524289396764946	0.0
37	4.816696597084132E-4	0.0	0.0	31.06038772802041	0.0
38	4.816696597084132E-4	0.0	0.0	31.605557504533717	0.0
39	4.816696597084132E-4	0.0	0.0	32.10151669748014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	30	2.1656488E-6	45.000004	40
CTTCGAA	20	7.033514E-4	45.0	19
TAATACG	40	6.8157533E-9	45.0	1
CGAACCG	20	7.033514E-4	45.0	31
ACTGCGT	20	7.033514E-4	45.0	23
GTCGACG	40	6.8157533E-9	45.0	2
ACGCATA	20	7.033514E-4	45.0	42
CGTGCCG	20	7.033514E-4	45.0	14
CGTGCCA	20	7.033514E-4	45.0	24
CGTGACG	40	6.8157533E-9	45.0	18
CGCATCG	25	3.8909944E-5	45.0	21
ACTCTCG	20	7.033514E-4	45.0	12
CCGGATA	25	3.8909944E-5	45.0	27
GCACGAC	35	1.212029E-7	45.0	9
ATTCGAT	20	7.033514E-4	45.0	14
TCCTACG	20	7.033514E-4	45.0	21
CCGTATC	20	7.033514E-4	45.0	42
GTTAACG	20	7.033514E-4	45.0	1
TACGATC	20	7.033514E-4	45.0	11
CATACGT	20	7.033514E-4	45.0	11
>>END_MODULE