FastQCFastQC Report
Sat 18 Jun 2016
SRR3550160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544659
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC184730.4064771416293279No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT157340.34620859342802174Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC139490.3069317191894926No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC64540.14201285508989783No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC61640.13563173826683148No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC51350.11298977547050285No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.11129547893472316No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG45930.10106368816670294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACA302.1669239E-645.00000424
CGATCGA207.0354895E-445.010
AACCGTA403.4612276E-739.37527
CGACTAT403.4612276E-739.37511
AACGGGA14700.039.3367354
TTACGCG1100.038.8636361
CTAACGG2650.038.2075462
CACAACG14000.037.9285712
TATCTCG1250.037.8000031
ACAACGA14000.037.60714313
CGCGAAA301.1404525E-437.50000434
TAGTTCG301.1404525E-437.50000424
ATACCGG1800.037.5000042
CGTTCGG3650.036.3698645
GTATACG1300.036.3461531
CCATCGA7550.036.05960531
TACGATA751.8189894E-1236.00000431
AATAGCG1500.036.0000041
CGCATAT504.8843503E-836.029
CATACGT250.002107762736.026