##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4544659
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.990743419913354	33.0	31.0	34.0	30.0	34.0
2	32.15549461466746	33.0	31.0	34.0	30.0	34.0
3	32.21179564847439	34.0	31.0	34.0	30.0	34.0
4	35.78819819044729	37.0	35.0	37.0	35.0	37.0
5	35.79958012251304	37.0	35.0	37.0	35.0	37.0
6	35.828205152465785	37.0	35.0	37.0	35.0	37.0
7	36.0370166386521	37.0	35.0	37.0	35.0	37.0
8	36.01732385202058	37.0	35.0	37.0	35.0	37.0
9	37.641221486584584	39.0	37.0	39.0	35.0	39.0
10	37.28276268912585	39.0	37.0	39.0	34.0	39.0
11	37.09388515177926	39.0	37.0	39.0	33.0	39.0
12	36.85371641744738	39.0	35.0	39.0	33.0	39.0
13	36.763511189728426	39.0	35.0	39.0	33.0	39.0
14	37.82914273656176	40.0	36.0	41.0	33.0	41.0
15	37.863800342335914	40.0	36.0	41.0	33.0	41.0
16	37.929893750004126	40.0	36.0	41.0	33.0	41.0
17	37.89520291841478	40.0	36.0	41.0	33.0	41.0
18	37.8231095006248	40.0	36.0	41.0	33.0	41.0
19	37.79073941521245	40.0	36.0	41.0	33.0	41.0
20	37.660886988440716	39.0	36.0	41.0	33.0	41.0
21	37.55695597843535	39.0	35.0	41.0	33.0	41.0
22	37.560782448144074	39.0	35.0	41.0	33.0	41.0
23	37.56427093869969	39.0	35.0	41.0	33.0	41.0
24	37.59294679754851	39.0	35.0	41.0	33.0	41.0
25	37.54537161093935	39.0	35.0	41.0	33.0	41.0
26	37.45756612322289	39.0	35.0	41.0	33.0	41.0
27	37.386511507243995	39.0	35.0	41.0	33.0	41.0
28	37.29556056901079	39.0	35.0	41.0	32.0	41.0
29	37.21008748951242	39.0	35.0	41.0	32.0	41.0
30	37.10761819533655	39.0	35.0	41.0	32.0	41.0
31	36.99808632506861	39.0	35.0	41.0	31.0	41.0
32	36.90957055303819	39.0	35.0	41.0	31.0	41.0
33	36.820632087027874	39.0	35.0	41.0	31.0	41.0
34	36.64616399162181	39.0	35.0	41.0	31.0	41.0
35	36.5938236510154	39.0	35.0	41.0	31.0	41.0
36	36.523661731276206	39.0	35.0	40.0	30.0	41.0
37	36.434923280272514	38.0	35.0	40.0	30.0	41.0
38	36.350885511982305	38.0	35.0	40.0	30.0	41.0
39	36.338766010827214	38.0	35.0	40.0	30.0	41.0
40	36.238450013521366	38.0	35.0	40.0	30.0	41.0
41	36.14718662060234	38.0	35.0	40.0	30.0	41.0
42	36.05668830158655	38.0	35.0	40.0	30.0	41.0
43	35.971996138764204	38.0	35.0	40.0	30.0	41.0
44	35.890107046535284	38.0	35.0	40.0	30.0	41.0
45	35.77272838292158	38.0	35.0	40.0	29.0	41.0
46	35.675838605272695	38.0	34.0	40.0	29.0	41.0
47	35.644893709297	38.0	34.0	40.0	29.0	41.0
48	35.61046428345889	37.0	34.0	40.0	29.0	41.0
49	35.556206527266404	37.0	34.0	40.0	29.0	41.0
50	35.43889387520604	37.0	34.0	40.0	29.0	41.0
51	34.03004273808002	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	12.0
8	101.0
9	249.0
10	311.0
11	224.0
12	117.0
13	96.0
14	130.0
15	188.0
16	331.0
17	768.0
18	1408.0
19	2499.0
20	4087.0
21	6522.0
22	9771.0
23	14256.0
24	20840.0
25	27749.0
26	35484.0
27	43572.0
28	53624.0
29	66618.0
30	84449.0
31	107537.0
32	140256.0
33	194235.0
34	347365.0
35	401946.0
36	376879.0
37	542012.0
38	887460.0
39	1173341.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67302761329288	20.606210498961527	25.596287862301658	12.124474025443934
2	31.117604203087623	24.544745821413663	25.684083228246607	18.653566747252103
3	31.799767595324536	24.79983646737852	26.29684647406989	17.103549463227054
4	29.22753940394648	25.648018916270725	25.723580140996276	19.40086153878652
5	25.290324312561186	29.965922635779712	24.258959803144748	20.484793248514354
6	26.803683180630273	33.61226881928875	23.320319522322798	16.26372847775818
7	80.34675428893566	6.1647089473599666	8.168973733782886	5.319563029921497
8	80.6335304805047	5.293642493309179	8.45418765192284	5.618639374263283
9	74.81626234223513	7.289347781648744	10.868604223111129	7.025785653004989
10	43.2645881682212	24.201771794099404	17.21141234138799	15.322227696291405
11	33.46482101297369	24.14753230110334	22.582398371362956	19.805248314560014
12	29.89408006189243	21.243001950201325	28.212061675034366	20.65085631287188
13	26.03592480755982	22.16943009365499	29.246880789075703	22.547764309709486
14	21.300740055524518	25.240529597490152	30.000402670475385	23.458327676509942
15	21.68813985823799	23.841480735958406	32.860353219020396	21.61002618678321
16	24.42841146057383	24.6275023054535	29.16326615484242	21.78082007913025
17	23.246738644197507	24.778888801117972	27.57456610055892	24.3998064541256
18	25.11017878349069	24.2042802331264	28.572154698515334	22.113386284867577
19	24.80223488714995	27.139946033354757	25.51449954771084	22.54331953178445
20	27.45141054587374	26.16290903233884	25.88070964180151	20.50497077998591
21	25.29111645120129	28.2223154696535	26.233233340499257	20.253334738645957
22	24.03614880676416	25.021789313565662	26.68407024597445	24.25799163369573
23	24.69681443646267	25.175552225150444	26.78966672747064	23.33796661091624
24	25.220770139189764	22.948410430793597	27.889661248511715	23.941158181504925
25	23.402481902382554	24.667703341438816	25.35708399684113	26.5727307593375
26	22.237113939681723	26.219832995170815	26.88593357609449	24.657119489052974
27	23.70263643542893	25.30002801090247	26.440619637248915	24.55671591641969
28	20.62909450412011	27.04975224763838	27.321499808896554	24.999653439344954
29	24.6692436110168	26.204298276284316	25.56997125636929	23.556486856329595
30	25.736320370791294	24.574142966501995	26.3315465472767	23.357990115430002
31	26.2916755690581	25.367843880035885	25.26966269636512	23.070817854540902
32	28.16550152607709	25.737882644220388	24.237660075266373	21.858955754436142
33	26.794595590120185	25.325398451236936	24.520849639103837	23.359156319539046
34	22.997919095800146	25.285175411400502	29.25682652977924	22.46007896302011
35	25.035387693554124	26.120925684413287	26.14097119277816	22.70271542925443
36	24.06470980551016	29.978816892532535	24.88492535963644	21.071547942320866
37	25.520044518191575	27.372394716523285	26.952275187203263	20.155285578081873
38	25.542554457881216	27.80789493777201	24.023496592373597	22.626054011973178
39	25.320865657907447	25.693192822607813	25.162657968397628	23.82328355108711
40	25.40621859637874	23.38611103715372	27.584753883624714	23.622916482842825
41	22.25317675099496	26.003029050144356	26.087545842273315	25.65624835658737
42	23.8279703713744	25.229285629570885	26.13533820689297	24.807405792161745
43	24.381169192232026	24.140491068746854	26.56014455650028	24.918195182520844
44	23.27477155051677	23.65094938916209	26.337465583226376	26.736813477094763
45	24.62827244024249	22.134157920319215	27.05050037857626	26.187069260862035
46	24.22100315997306	24.351684031739236	26.687810900663834	24.739501907623872
47	22.11822713211266	24.558058151337647	28.955131727154885	24.368582989394806
48	22.83319826636058	22.97283470553016	29.257332618354866	24.936634409754397
49	24.46566398050987	22.548006352071738	29.13098650525815	23.855343162160242
50	21.681428683648214	22.696972424113667	29.25332791745211	26.368270974786007
51	21.20220681023593	21.8292285515811	27.80384622916703	29.164718409015943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1297.0
1	3337.0
2	5377.0
3	8684.5
4	11992.0
5	8987.0
6	5982.0
7	6117.5
8	6253.0
9	6665.0
10	7077.0
11	7560.0
12	8043.0
13	8420.0
14	8797.0
15	8833.5
16	8870.0
17	9441.5
18	10013.0
19	10468.5
20	10924.0
21	11695.5
22	12467.0
23	13613.0
24	14759.0
25	19816.0
26	27337.5
27	29802.0
28	34878.0
29	39954.0
30	50238.5
31	60523.0
32	67435.0
33	74347.0
34	82608.0
35	90869.0
36	98274.5
37	105680.0
38	115161.5
39	124643.0
40	143135.0
41	161627.0
42	181428.5
43	201230.0
44	227996.0
45	254762.0
46	300603.0
47	346444.0
48	395324.5
49	444205.0
50	445367.0
51	446529.0
52	398890.0
53	351251.0
54	323683.5
55	296116.0
56	273404.5
57	250693.0
58	248506.5
59	246320.0
60	240828.0
61	235336.0
62	217497.0
63	199658.0
64	172751.5
65	145845.0
66	120179.0
67	94513.0
68	81714.5
69	68916.0
70	56804.0
71	44692.0
72	38180.5
73	31669.0
74	27909.5
75	19966.0
76	15782.0
77	12273.0
78	8764.0
79	7649.0
80	6534.0
81	5384.5
82	4235.0
83	2774.5
84	1314.0
85	1029.0
86	744.0
87	588.5
88	433.0
89	352.5
90	272.0
91	164.5
92	57.0
93	39.0
94	21.0
95	12.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4544659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.46007708818637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.1188177033441	12.390185488716602
2	12.298946332643323	4.5407899480812555
3	5.818304641426432	3.222190566098535
4	3.1726356600615095	2.3426839542985807
5	1.9765928759512115	1.8244028431009685
6	1.3720440877808306	1.5196823777294688
7	0.9774760842004744	1.2630998706339516
8	0.7119882761724001	1.0514686771221946
9	0.5322588828861804	0.8842986008055765
>10	3.599633739657282	13.993825599548055
>50	1.1528569589096402	15.647356267387085
>100	1.2504296812129923	36.29519412798148
>500	0.011529648482344347	1.4980261961444559
>1k	0.005644723736143414	1.9534112610972691
>5k	4.8040202009731183E-4	0.5057346015899783
>10k+	3.603015150729839E-4	1.0676496196645038
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	18473	0.4064771416293279	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	15734	0.34620859342802174	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	13949	0.3069317191894926	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	6454	0.14201285508989783	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC	6164	0.13563173826683148	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	5135	0.11298977547050285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5058	0.11129547893472316	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	4593	0.10106368816670294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.760308089121758E-4	0.0	0.0	0.37210712618922565	0.0
2	1.760308089121758E-4	0.0	0.0	1.4021514045388224	0.0
3	1.760308089121758E-4	0.0	0.0	1.9827450200334062	0.0
4	1.760308089121758E-4	0.0	0.0	2.5719201374624587	0.0
5	1.760308089121758E-4	0.0	0.0	4.084420855338101	0.0
6	1.760308089121758E-4	0.0	0.0	4.992321756153762	0.0
7	1.760308089121758E-4	0.0	0.0	5.981438871431278	0.0
8	1.760308089121758E-4	0.0	0.0	7.509738354406788	0.0
9	1.760308089121758E-4	0.0	0.0	8.136848111156414	0.0
10	1.760308089121758E-4	0.0	0.0	9.348182118834439	0.0
11	1.760308089121758E-4	0.0	0.0	11.030684590417016	0.0
12	1.760308089121758E-4	0.0	0.0	12.261756052544316	0.0
13	1.760308089121758E-4	0.0	0.0	12.78227915449762	0.0
14	1.760308089121758E-4	0.0	0.0	12.996860710561563	0.0
15	2.2003851114021976E-4	0.0	0.0	13.418476501757338	0.0
16	2.2003851114021976E-4	0.0	0.0	14.1807559159004	0.0
17	2.2003851114021976E-4	0.0	0.0	15.088744832120518	0.0
18	2.4204236225424172E-4	0.0	0.0	16.105124718928305	0.0
19	2.4204236225424172E-4	0.0	0.0	16.757362873650145	0.0
20	2.4204236225424172E-4	0.0	0.0	17.40174565352428	0.0
21	2.4204236225424172E-4	0.0	0.0	18.16373901760286	0.0
22	2.4204236225424172E-4	0.0	0.0	18.912332036353003	0.0
23	2.860500644822857E-4	0.0	0.0	19.619733845817695	0.0
24	2.860500644822857E-4	0.0	0.0	20.224861755304413	0.0
25	2.860500644822857E-4	0.0	0.0	20.75130389320739	0.0
26	2.860500644822857E-4	0.0	0.0	21.240933588196608	0.0
27	3.080539155963077E-4	0.0	0.0	21.71265214837901	0.0
28	3.520616178243516E-4	0.0	0.0	22.205758451844243	0.0
29	3.520616178243516E-4	0.0	0.0	22.705883983814847	0.0
30	3.7406546893837357E-4	0.0	0.0	23.295587193670634	0.0
31	3.7406546893837357E-4	0.0	0.0	23.7943924945744	0.0
32	3.7406546893837357E-4	0.0	0.0	24.29685043476309	0.0
33	3.960693200523956E-4	0.0	0.0	24.815239163158335	0.0
34	4.1807317116641755E-4	0.0	0.0	25.29292076699264	0.0
35	4.1807317116641755E-4	0.0	0.0	25.790757898447385	0.0
36	4.1807317116641755E-4	0.0	0.0	26.275722777000432	0.0
37	4.1807317116641755E-4	0.0	0.0	26.771623569557143	0.0
38	4.8408472450848345E-4	0.0	0.0	27.3079234327592	0.0
39	5.060885756225054E-4	0.0	0.0	27.890211344789567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAACA	30	2.1669239E-6	45.000004	24
CGATCGA	20	7.0354895E-4	45.0	10
AACCGTA	40	3.4612276E-7	39.375	27
CGACTAT	40	3.4612276E-7	39.375	11
AACGGGA	1470	0.0	39.336735	4
TTACGCG	110	0.0	38.863636	1
CTAACGG	265	0.0	38.207546	2
CACAACG	1400	0.0	37.92857	12
TATCTCG	125	0.0	37.800003	1
ACAACGA	1400	0.0	37.607143	13
CGCGAAA	30	1.1404525E-4	37.500004	34
TAGTTCG	30	1.1404525E-4	37.500004	24
ATACCGG	180	0.0	37.500004	2
CGTTCGG	365	0.0	36.36986	45
GTATACG	130	0.0	36.346153	1
CCATCGA	755	0.0	36.059605	31
TACGATA	75	1.8189894E-12	36.000004	31
AATAGCG	150	0.0	36.000004	1
CGCATAT	50	4.8843503E-8	36.0	29
CATACGT	25	0.0021077627	36.0	26
>>END_MODULE