FastQCFastQC Report
Sat 18 Jun 2016
SRR3550159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700739
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC235380.8715392342614373No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT183970.6811839278064263No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC180540.6684837002020558No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC87830.3252072858576856No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC69420.2570407581036154No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG47490.1758407606214447No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG37200.1377400778083332No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG35460.13129739674955634No Hit
GGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG35360.13092712772318985No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC33940.12566930754878572No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG30650.11348745658132829No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC27020.10004669092422482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAA302.1665946E-645.00000423
ACGTAAC302.1665946E-645.00000415
CGGTCGA302.1665946E-645.00000432
TATCGAT600.045.00000411
TACGTGA302.1665946E-645.00000419
GTCGACG406.8212103E-945.01
CACGGAT207.0349814E-445.020
CTATACG406.8212103E-945.026
TCCTACG253.89221E-545.045
ATCCGTT453.8562575E-1045.043
CTAACCG253.89221E-545.013
ATTCACG253.89221E-545.01
CTACGTT253.89221E-545.030
ATATACG207.0349814E-445.019
TCGCTAA406.8212103E-945.019
TATTACG207.0349814E-445.01
CAAATCG207.0349814E-445.037
CGTACAT253.89221E-545.034
CCGCGTA253.89221E-545.017
GAACCGT406.8212103E-945.020