##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3611458
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.008064886813024	33.0	31.0	34.0	30.0	34.0
2	32.17169769107103	34.0	31.0	34.0	30.0	34.0
3	32.238472107387096	34.0	31.0	34.0	30.0	34.0
4	35.82065359752211	37.0	35.0	37.0	35.0	37.0
5	35.815008785925244	37.0	35.0	37.0	35.0	37.0
6	35.836974152821384	37.0	35.0	37.0	35.0	37.0
7	36.010903075710694	37.0	35.0	37.0	35.0	37.0
8	35.97809804239728	37.0	35.0	37.0	35.0	37.0
9	37.57739062727574	39.0	37.0	39.0	35.0	39.0
10	37.27788776721202	39.0	37.0	39.0	34.0	39.0
11	37.12949589888627	39.0	37.0	39.0	34.0	39.0
12	36.93143960140198	39.0	37.0	39.0	33.0	39.0
13	36.83072376862752	39.0	35.0	39.0	33.0	39.0
14	37.942648370824195	40.0	37.0	41.0	33.0	41.0
15	37.986144654042775	40.0	37.0	41.0	33.0	41.0
16	38.05215012883993	40.0	37.0	41.0	33.0	41.0
17	38.049441250597404	40.0	37.0	41.0	33.0	41.0
18	37.98051174899445	40.0	36.0	41.0	33.0	41.0
19	37.933572258074165	40.0	36.0	41.0	33.0	41.0
20	37.784102985553204	40.0	36.0	41.0	33.0	41.0
21	37.67128013118248	39.0	36.0	41.0	33.0	41.0
22	37.65401618958327	39.0	36.0	41.0	33.0	41.0
23	37.6604504330384	39.0	36.0	41.0	33.0	41.0
24	37.68356519721398	39.0	36.0	41.0	33.0	41.0
25	37.60467905206152	39.0	36.0	41.0	33.0	41.0
26	37.51777260042897	39.0	35.0	41.0	33.0	41.0
27	37.43160878514993	39.0	35.0	41.0	33.0	41.0
28	37.35195868261516	39.0	35.0	41.0	32.0	41.0
29	37.30502749858921	39.0	35.0	41.0	32.0	41.0
30	37.237056058799524	39.0	35.0	41.0	32.0	41.0
31	37.11009736233953	39.0	35.0	41.0	32.0	41.0
32	37.01098005293153	39.0	35.0	41.0	31.0	41.0
33	36.94334338098353	39.0	35.0	41.0	31.0	41.0
34	36.83005035639346	39.0	35.0	41.0	31.0	41.0
35	36.74356312602833	39.0	35.0	41.0	31.0	41.0
36	36.646031602748806	39.0	35.0	41.0	31.0	41.0
37	36.47238539116335	39.0	35.0	40.0	30.0	41.0
38	36.32075715680482	38.0	35.0	40.0	30.0	41.0
39	36.30583852837275	38.0	35.0	40.0	30.0	41.0
40	36.17330175236705	38.0	35.0	40.0	30.0	41.0
41	36.09496635430898	38.0	35.0	40.0	30.0	41.0
42	36.00083511977711	38.0	35.0	40.0	30.0	41.0
43	35.91661345639351	38.0	35.0	40.0	30.0	41.0
44	35.79726138307576	38.0	35.0	40.0	29.0	41.0
45	35.64112748923011	38.0	34.0	40.0	29.0	41.0
46	35.534309134980944	38.0	34.0	40.0	28.0	41.0
47	35.471400193495256	38.0	34.0	40.0	28.0	41.0
48	35.42859919733249	37.0	34.0	40.0	28.0	41.0
49	35.35007467898007	37.0	34.0	40.0	28.0	41.0
50	35.198455305308826	37.0	34.0	40.0	27.0	41.0
51	33.670355020050074	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	11.0
8	68.0
9	196.0
10	238.0
11	151.0
12	94.0
13	67.0
14	76.0
15	121.0
16	296.0
17	537.0
18	1108.0
19	1920.0
20	3235.0
21	5094.0
22	7701.0
23	11400.0
24	16499.0
25	22319.0
26	28688.0
27	35636.0
28	43490.0
29	54008.0
30	67945.0
31	85674.0
32	110059.0
33	150541.0
34	257852.0
35	319027.0
36	304579.0
37	437029.0
38	696511.0
39	949063.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.455401668799695	21.218521716160065	25.861964890634198	10.464111724406044
2	31.01849723851143	25.68117918026459	25.136385360150943	18.16393822107304
3	31.891828729560196	24.361518256615472	26.049479185414864	17.69717382840947
4	29.464471136034255	26.16754784355792	25.2659175324758	19.10206348793202
5	25.219288165610678	30.364550826840574	23.643774896454563	20.77238611109419
6	26.30444546219283	36.35515074521149	22.707504835996986	14.63289895659869
7	80.89034954857567	6.791855256242769	7.471636109294362	4.846159085887196
8	81.3895938980877	5.787662489775598	7.5339101271564	5.288833484980304
9	74.31627337213945	8.306478989925953	10.358891062833903	7.018356575100694
10	41.83052938730009	24.796688761159622	16.97392022833991	16.398861623200382
11	32.220172572960834	24.873278326925025	22.51461874954658	20.391930350567556
12	27.074300739479735	22.604222449769594	28.591582679350005	21.72989413140067
13	23.929005958258408	24.250538148304646	31.0760640162505	20.744391877186445
14	21.23095436801425	26.711317146703628	29.63296264278859	22.424765842493528
15	20.80223555140334	25.202203652929096	33.16738004429236	20.828180751375207
16	22.96416018128966	24.44514099291754	30.182962116685285	22.40773670910751
17	23.292919369407038	24.895346976207396	26.667595192855625	25.14413846152994
18	23.371391831221626	25.27735335700983	28.700846029498333	22.65040878227021
19	24.85807671029263	25.416272319932837	26.90032668246453	22.82532428731
20	25.345802166327285	26.398064161344255	26.18083887449335	22.075294797835113
21	24.681056792021394	26.314579873281097	27.875694525590493	21.128668809107015
22	23.132070205440574	24.784588385078823	26.84154156022305	25.241799849257557
23	23.364386350332747	25.42701590327231	26.85427879820283	24.354318948192116
24	23.786736547953762	24.6674057956648	28.014115074853425	23.531742581528015
25	22.96748293902352	26.048731564924747	26.38828971567716	24.595495780374574
26	21.784581185770403	28.484839087149844	25.766767881559193	23.963811845520564
27	23.879663005910633	26.629854202928566	26.25903443983012	23.23144835133068
28	21.141184529904542	26.749362722756292	28.212317573677996	23.897135173661162
29	22.463974383753044	26.53299581498663	27.142500341967153	23.860529459293172
30	24.476374915615796	26.55509215391678	24.835786543828007	24.13274638663941
31	25.437316452247266	28.04258003277347	23.38421213814476	23.135891376834508
32	24.604522605551555	28.637547494668357	23.735039975544503	23.022889924235585
33	24.779825765660295	26.38349940661085	23.94323843721843	24.893436390510427
34	22.151828984304952	26.871501759123323	26.377906097758856	24.59876315881287
35	23.68339878243081	26.247737063535006	26.07913479818954	23.989729355844645
36	25.218402096881647	28.28896251873897	23.529776616535482	22.9628587678439
37	24.56312658211725	28.040392550598682	25.78678749690568	21.60969337037839
38	22.1495861228346	29.018667806741767	25.026872803172566	23.804873267251068
39	24.183446131728516	25.017347564335513	25.935204009017966	24.86400229491801
40	23.921474374061667	25.026568217046965	26.875212171926133	24.176745236965235
41	21.953128071820302	26.30117808375454	24.564234168028538	27.181459676396624
42	21.641038051667774	26.668758158062477	26.014922504982753	25.675281285286992
43	24.137785902535764	23.831621467008617	27.33818308284355	24.692409547612073
44	22.9710825932352	24.991651571193685	26.06623142232306	25.971034413248056
45	22.856142865291527	24.169213652768494	26.85001459244438	26.1246288894956
46	24.043198065711966	25.558514040589703	25.729940650008942	24.66834724368939
47	22.2294430670383	25.122623605203216	28.259362285259858	24.388571042498626
48	20.95178733907469	24.92115372794035	28.50494177143968	25.622117161545283
49	22.60430551871294	23.991916838019435	28.47135422867994	24.93242341458768
50	21.816008936003133	23.725653184946356	28.506243184885438	25.952094694165073
51	21.798370630365905	24.296558342918566	25.533897943711377	28.371173083004148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1025.0
1	3114.0
2	5203.0
3	6072.5
4	6942.0
5	5653.5
6	4365.0
7	4255.0
8	4145.0
9	4501.5
10	4858.0
11	5221.0
12	5584.0
13	5701.5
14	5819.0
15	6110.0
16	6401.0
17	6623.5
18	6846.0
19	7613.0
20	8380.0
21	8826.0
22	9272.0
23	11855.5
24	14439.0
25	18488.5
26	25881.0
27	29224.0
28	37252.5
29	45281.0
30	51539.5
31	57798.0
32	61838.5
33	65879.0
34	71608.5
35	77338.0
36	81895.0
37	86452.0
38	96288.0
39	106124.0
40	120635.5
41	135147.0
42	157580.0
43	180013.0
44	207935.0
45	235857.0
46	265982.5
47	296108.0
48	332971.5
49	369835.0
50	359449.5
51	349064.0
52	306214.5
53	263365.0
54	233066.5
55	202768.0
56	192029.5
57	181291.0
58	174909.5
59	168528.0
60	161620.0
61	154712.0
62	145748.5
63	136785.0
64	124463.5
65	112142.0
66	95711.0
67	79280.0
68	67610.5
69	55941.0
70	49033.5
71	42126.0
72	35307.5
73	28489.0
74	24966.5
75	15842.0
76	10240.0
77	9088.0
78	7936.0
79	6030.5
80	4125.0
81	2719.5
82	1314.0
83	851.5
84	389.0
85	411.5
86	434.0
87	271.0
88	108.0
89	80.0
90	52.0
91	37.5
92	23.0
93	21.5
94	20.0
95	13.5
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3611458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.197992138636405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44041439821365	11.77037708785496
2	11.522781234283494	3.963374021648692
3	5.306599831616185	2.7378858656107323
4	3.2172959571064745	2.2132412231193532
5	2.0204840260225136	1.737413419788781
6	1.3911903284626923	1.4355408199349027
7	0.9766299612276396	1.1757252076884803
8	0.7406840169933125	1.0190622321171696
9	0.582242799750308	0.9012066383595101
>10	3.524463993959468	12.107639603196011
>50	0.7919531326728673	9.937884495577451
>100	1.4640940812914232	46.15443770273175
>500	0.01351382913226324	1.578012834964206
>1k	0.007001140393823125	2.18214071916885
>5k	1.628172184610029E-4	0.14851885505545467
>10k+	4.884516553830087E-4	0.9375392731837733
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	12431	0.34421001157981074	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	10564	0.29251343917055106	TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	10487	0.2903813362913261	TruSeq Adapter, Index 21 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5304	0.14686589183648266	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4783	0.13243958534198652	No Hit
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	4672	0.12936603443816874	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2151718225713824E-4	0.0	0.0	0.33784139258991797	0.0
2	2.2151718225713824E-4	0.0	0.0	1.3320105065599546	0.0
3	2.2151718225713824E-4	0.0	0.0	1.8504160923372222	0.0
4	2.2151718225713824E-4	0.0	0.0	2.4027691862953966	0.0
5	2.2151718225713824E-4	0.0	0.0	3.7962784005794887	0.0
6	2.2151718225713824E-4	0.0	0.0	4.611683148468015	0.0
7	2.2151718225713824E-4	0.0	0.0	5.433622653233126	0.0
8	2.2151718225713824E-4	0.0	0.0	6.823199937532155	0.0
9	2.2151718225713824E-4	0.0	0.0	7.370901170662929	0.0
10	2.4920683003928055E-4	0.0	0.0	8.48419114939174	0.0
11	2.4920683003928055E-4	0.0	0.0	10.085400411689683	0.0
12	2.4920683003928055E-4	0.0	0.0	11.277245921176434	0.0
13	2.4920683003928055E-4	0.0	0.0	11.776850236109627	0.0
14	2.7689647782142284E-4	0.0	0.0	11.979289251044868	0.0
15	2.7689647782142284E-4	0.0	0.0	12.387323900762517	0.0
16	2.7689647782142284E-4	0.0	0.0	13.181684516336615	0.0
17	2.7689647782142284E-4	0.0	0.0	14.131633262798571	0.0
18	2.7689647782142284E-4	0.0	0.0	15.267933338834343	0.0
19	3.876550689499919E-4	0.0	0.0	15.902109341988748	0.0
20	3.876550689499919E-4	0.0	0.0	16.539912689002612	0.0
21	3.876550689499919E-4	0.0	0.0	17.273079182978176	0.0
22	4.153447167321342E-4	0.0	0.0	17.97573168509782	0.0
23	4.153447167321342E-4	0.0	0.0	18.640117093982543	0.0
24	4.153447167321342E-4	0.0	0.0	19.199835634250764	0.0
25	4.153447167321342E-4	0.0	0.0	19.723945287471153	0.0
26	4.153447167321342E-4	0.0	0.0	20.20159171171311	0.0
27	4.153447167321342E-4	0.0	0.0	20.6539021082344	0.0
28	4.7072401229641877E-4	0.0	0.0	21.147442390303308	0.0
29	4.7072401229641877E-4	0.0	0.0	21.65180932465503	0.0
30	4.7072401229641877E-4	0.0	0.0	22.18954228458423	0.0
31	6.645515467714148E-4	0.0	0.0	22.68911890986964	0.0
32	6.922411945535571E-4	0.0	0.0	23.17352160817044	0.0
33	6.922411945535571E-4	0.0	0.0	23.646183895811607	0.0
34	6.922411945535571E-4	0.0	0.0	24.112339116223975	0.0
35	6.922411945535571E-4	0.0	0.0	24.587022748153238	0.0
36	6.922411945535571E-4	0.0	0.0	25.057054519255104	0.0
37	7.199308423356994E-4	0.0	0.0	25.491865058378085	0.0
38	7.753101378999838E-4	0.0	0.0	25.965081138974895	0.0
39	7.753101378999838E-4	0.0	0.0	26.458676800339365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	30	2.1667984E-6	45.000004	29
CGCGATC	20	7.0352975E-4	45.0	28
TACGCTA	35	1.2129021E-7	45.0	5
ATATCGT	20	7.0352975E-4	45.0	11
CGTAGCA	25	3.892472E-5	45.0	16
ACGTATC	45	3.8562575E-10	45.0	29
ACCCGAT	45	3.8562575E-10	45.0	29
TACGTAA	25	3.892472E-5	45.0	16
AGTCGAC	20	7.0352975E-4	45.0	13
TACGTCG	65	0.0	44.999996	1
ATATCGC	60	3.6379788E-12	41.250004	42
TACGGGT	400	0.0	41.0625	4
TACGGGC	470	0.0	40.69149	4
TACGCAC	50	1.0822987E-9	40.5	1
CGTAAGG	615	0.0	39.878048	2
TTACGCG	40	3.460973E-7	39.375	1
ACCGTTA	40	3.460973E-7	39.375	37
TTACGGG	830	0.0	38.76506	3
CGAACAT	30	1.14040624E-4	37.500004	13
AACGCAT	30	1.14040624E-4	37.500004	29
>>END_MODULE