FastQCFastQC Report
Sat 18 Jun 2016
SRR3550152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428240
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC20360.47543433588641887No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC17400.40631421632729314No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT12310.2874556323556884TruSeq Adapter, Index 21 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9810.2290771529983187No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC7580.17700354941154492No Hit
ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC5750.13427050252195033TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG4320.10087801232953485TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT207.028322E-445.00000416
GTCGCAA207.028322E-445.00000413
ATCTATG207.028322E-445.00000426
AATGCGG351.209537E-745.0000042
AGTGCGC207.028322E-445.00000413
CTAACGG207.028322E-445.0000042
GTAGACG207.028322E-445.0000041
TACGAAT207.028322E-445.00000412
ACGTGCC207.028322E-445.00000440
TTGCGTA207.028322E-445.00000414
AGCGATA207.028322E-445.00000434
TCCCGAC207.028322E-445.00000438
CGTAAGA207.028322E-445.00000440
CAACTCC207.028322E-445.00000426
GCCGTAA207.028322E-445.00000438
TGCACGT207.028322E-445.00000421
TTTCGGC253.8866925E-545.016
CTCGCGC253.8866925E-545.033
ACATGCG253.8866925E-545.01
TATAGCG253.8866925E-545.01