##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428240
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48160844386325	33.0	31.0	34.0	31.0	34.0
2	32.65032925462358	34.0	31.0	34.0	31.0	34.0
3	32.66957547169811	34.0	31.0	34.0	31.0	34.0
4	36.10690033626004	37.0	35.0	37.0	35.0	37.0
5	36.10498085185877	37.0	35.0	37.0	35.0	37.0
6	36.14900523071175	37.0	36.0	37.0	35.0	37.0
7	36.318744162152065	37.0	37.0	37.0	35.0	37.0
8	36.27079674948627	37.0	37.0	37.0	35.0	37.0
9	38.005655707080145	39.0	39.0	39.0	37.0	39.0
10	37.753787595740704	39.0	38.0	39.0	35.0	39.0
11	37.585104147207176	39.0	37.0	39.0	35.0	39.0
12	37.33462077339809	39.0	37.0	39.0	35.0	39.0
13	37.19224967308052	39.0	37.0	39.0	34.0	39.0
14	38.363704931813935	40.0	38.0	41.0	34.0	41.0
15	38.42393050625817	40.0	37.0	41.0	34.0	41.0
16	38.50367550906034	40.0	37.0	41.0	35.0	41.0
17	38.50835512796563	40.0	37.0	41.0	35.0	41.0
18	38.505422193162715	40.0	37.0	41.0	35.0	41.0
19	38.48726648608257	40.0	37.0	41.0	35.0	41.0
20	38.418356529049134	40.0	37.0	41.0	34.0	41.0
21	38.37676770035494	40.0	37.0	41.0	34.0	41.0
22	38.34257892770409	40.0	37.0	41.0	34.0	41.0
23	38.305461890528676	40.0	37.0	41.0	34.0	41.0
24	38.278444330282085	40.0	37.0	41.0	34.0	41.0
25	38.24347562114702	40.0	36.0	41.0	34.0	41.0
26	38.18975107416402	40.0	36.0	41.0	34.0	41.0
27	38.09857089482533	40.0	36.0	41.0	34.0	41.0
28	38.06652344479731	40.0	36.0	41.0	34.0	41.0
29	38.05886885858397	40.0	36.0	41.0	34.0	41.0
30	37.93117410797684	40.0	36.0	41.0	34.0	41.0
31	37.83978843639081	40.0	36.0	41.0	34.0	41.0
32	37.66388240239118	40.0	35.0	41.0	33.0	41.0
33	37.616542125910705	40.0	35.0	41.0	33.0	41.0
34	37.53312861946572	40.0	35.0	41.0	33.0	41.0
35	37.3951312348216	40.0	35.0	41.0	33.0	41.0
36	37.34108443863254	39.0	35.0	41.0	33.0	41.0
37	37.25623015131702	39.0	35.0	41.0	33.0	41.0
38	37.178547076405756	39.0	35.0	41.0	33.0	41.0
39	37.2045605268074	39.0	35.0	41.0	33.0	41.0
40	37.07744489071548	39.0	35.0	41.0	32.0	41.0
41	36.935610872408	39.0	35.0	41.0	32.0	41.0
42	36.88802307117504	39.0	35.0	41.0	32.0	41.0
43	36.858336446852235	39.0	35.0	41.0	32.0	41.0
44	36.79085793013264	39.0	35.0	41.0	32.0	41.0
45	36.7215393237437	39.0	35.0	41.0	32.0	41.0
46	36.61165000934056	39.0	35.0	41.0	31.0	41.0
47	36.49603493368205	39.0	35.0	41.0	31.0	41.0
48	36.43952923594246	38.0	35.0	41.0	31.0	41.0
49	36.427421539323745	38.0	35.0	40.0	31.0	41.0
50	36.311981599103305	38.0	35.0	40.0	31.0	41.0
51	35.308908555949934	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	7.0
11	8.0
12	6.0
13	9.0
14	9.0
15	6.0
16	18.0
17	43.0
18	60.0
19	119.0
20	210.0
21	382.0
22	501.0
23	852.0
24	1321.0
25	1856.0
26	2405.0
27	2870.0
28	3391.0
29	3965.0
30	4990.0
31	6267.0
32	8497.0
33	12504.0
34	27071.0
35	40903.0
36	28120.0
37	43959.0
38	86668.0
39	151062.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57738651223613	22.086213338314963	25.898795068186065	10.437605081262843
2	31.52367831122735	24.28684849617037	25.640762189426493	18.54871100317579
3	31.845694003362603	23.51485148514851	26.990706146086307	17.64874836540258
4	30.14944890715487	25.047870353073044	25.76288996824211	19.039790771529983
5	24.71744815991033	30.61017186624323	24.79077152998319	19.881608443863254
6	27.233327106295533	33.03007659256492	23.86745750046703	15.86913880067252
7	81.42373435456753	5.759620773398094	7.63006725200822	5.186577620026154
8	82.07360358677377	4.459882308985615	8.039884177096955	5.426629927143658
9	76.54843078647488	6.421632729310667	9.801046142350083	7.228890341864375
10	43.5195217634971	25.969316271249767	16.758359798243973	13.752802167009154
11	32.63380347468709	24.778862320194285	23.28507379039791	19.302260414720717
12	30.62861946572016	22.429712310853727	27.554408742761066	19.387259480665048
13	26.15192415467962	25.35424061274052	29.03652157668597	19.45731365589389
14	21.061787782551843	27.55884550719223	28.79600224173361	22.583364468522323
15	19.346161031197457	25.77059592751728	33.63791331963385	21.24532972165141
16	22.55674388193536	25.9854287315524	30.30963945451149	21.14818793200075
17	22.64477862880628	25.563702596674766	26.97179151877452	24.819727255744443
18	23.532832056790586	25.675789277040913	29.186203997758263	21.60517466841024
19	24.428357930132634	26.990472632168878	26.273818419577804	22.30735101812068
20	25.91864375116757	27.20390435269942	27.506538389688025	19.370913506444985
21	26.06178778255184	26.56781244162152	27.972165141042403	19.398234634784234
22	23.34742200635158	24.796142350084065	27.14972912385578	24.706706519708575
23	22.600177470577247	25.89249019241547	28.14940220437138	23.357930132635904
24	24.29618905286755	24.168923967868487	28.700495049504948	22.83439192975901
25	22.970764057537828	26.38847375303568	26.78801606575752	23.85274612366897
26	22.671632729310666	28.130487577059593	26.279189239678686	22.918690453951054
27	22.553708201008778	26.860405380160657	28.825424995329723	21.76046142350084
28	20.05394171492621	26.236222678871663	30.428031010648233	23.281804595553897
29	24.64225667849804	23.83266392677004	28.801373061834486	22.723706332897443
30	25.239818793200076	24.962404259293855	27.816411358116945	21.981365589389128
31	25.81076032131515	26.626424434896318	26.111759760881746	21.45105548290678
32	26.88702596674762	25.924948626938164	26.01765365215767	21.170371754156548
33	25.71642069867364	24.611899869232207	27.304315337194097	22.367364094900054
34	24.890715486643003	23.881234821595367	29.028348589575938	22.19970110218569
35	24.46525312908649	23.240939660003736	29.673314029516156	22.620493181393613
36	25.607369699234074	24.088361666355315	29.88254249953297	20.421726134877638
37	24.0652437885298	24.948626938165514	31.02699420885485	19.95913506444984
38	24.53016999813189	25.019848682981504	28.55688399028582	21.893097328600785
39	26.46413226228283	24.163086119932746	27.8817952549972	21.490986362787222
40	27.582663926770035	22.89930879880441	28.527227722772274	20.99079955165328
41	22.677470577246403	23.854147207173547	28.750700541752288	24.71768167382776
42	23.48659630113955	22.696385204558194	30.67555576312348	23.141462731178777
43	23.7903979077153	22.873622267887168	29.782131515038294	23.55384830935924
44	23.91182514477863	22.96889594619839	28.371473939846815	24.747804969176162
45	24.373248645619277	22.524985989164954	27.183355127965626	25.91841023725014
46	25.78460676256305	24.043760508126287	27.92476181580422	22.246870913506445
47	22.43718475621147	23.33317765738838	31.900803287875956	22.328834298524193
48	22.78208481225481	21.343639080889222	31.141182514477862	24.733093592378104
49	23.68765178404633	20.565103680179337	32.682374369512424	23.06487016626191
50	23.552680739772093	21.15169064076219	30.57000747244536	24.725621147020362
51	22.248038483093595	21.957080141976462	27.778815617410796	28.016065757519147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	337.0
2	543.0
3	718.5
4	894.0
5	681.0
6	468.0
7	494.5
8	521.0
9	564.5
10	608.0
11	638.5
12	669.0
13	657.5
14	646.0
15	678.5
16	711.0
17	736.0
18	761.0
19	727.5
20	694.0
21	741.5
22	789.0
23	935.5
24	1082.0
25	1201.0
26	1595.5
27	1871.0
28	2672.0
29	3473.0
30	3969.0
31	4465.0
32	5330.0
33	6195.0
34	7933.5
35	9672.0
36	10104.0
37	10536.0
38	11823.0
39	13110.0
40	15704.5
41	18299.0
42	21429.0
43	24559.0
44	27106.0
45	29653.0
46	34041.5
47	38430.0
48	44574.0
49	50718.0
50	49731.0
51	48744.0
52	41608.0
53	34472.0
54	30286.0
55	26100.0
56	23716.0
57	21332.0
58	20243.5
59	19155.0
60	17378.5
61	15602.0
62	14342.0
63	13082.0
64	11126.5
65	9171.0
66	7658.0
67	6145.0
68	5504.5
69	4864.0
70	4139.0
71	3414.0
72	2778.5
73	2143.0
74	1745.0
75	1073.5
76	800.0
77	644.5
78	489.0
79	401.0
80	313.0
81	181.0
82	49.0
83	64.5
84	80.0
85	64.0
86	48.0
87	55.0
88	62.0
89	33.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.493902730265834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55649391297068	22.535932590139577
2	11.790396537559014	7.426512034102932
3	4.312029583854529	4.074079208518295
4	2.1074970308999097	2.6549322598194323
5	1.297134504651562	2.0425913958783926
6	0.8938597742288871	1.689067967043717
7	0.68670006912618	1.5138805627348717
8	0.5353355849685978	1.3487845472840783
9	0.48093135411994187	1.3631764757929634
>10	5.864671890340137	43.38415688914554
>50	0.43028363590622626	8.110389283405906
>100	0.04019950923691387	2.14007554646689
>500	0.002233306068717437	0.5425213425201997
>1k	0.002233306068717437	1.173899897147208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	2036	0.47543433588641887	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1740	0.40631421632729314	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	1231	0.2874556323556884	TruSeq Adapter, Index 21 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	981	0.2290771529983187	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	758	0.17700354941154492	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	575	0.13427050252195033	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	432	0.10087801232953485	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3007659256491687	0.0
2	0.0	0.0	0.0	1.565477302447226	0.0
3	0.0	0.0	0.0	2.1681767233327105	0.0
4	0.0	0.0	0.0	2.7792826452456567	0.0
5	0.0	0.0	0.0	4.488137492994582	0.0
6	0.0	0.0	0.0	5.340930319447039	0.0
7	0.0	0.0	0.0	6.2532691948440124	0.0
8	0.0	0.0	0.0	7.628199140668784	0.0
9	0.0	0.0	0.0	8.153138427050251	0.0
10	0.0	0.0	0.0	9.40850924715113	0.0
11	0.0	0.0	0.0	11.334765552026902	0.0
12	0.0	0.0	0.0	12.765271810199888	0.0
13	0.0	0.0	0.0	13.348823089856156	0.0
14	0.0	0.0	0.0	13.550579114515225	0.0
15	0.0	0.0	0.0	13.9963571828881	0.0
16	0.0	0.0	0.0	14.877171679432093	0.0
17	0.0	0.0	0.0	15.9046329161218	0.0
18	0.0	0.0	0.0	16.985101812068	0.0
19	2.335139174294788E-4	0.0	0.0	17.686344106108724	0.0
20	4.670278348589576E-4	0.0	0.0	18.435223239305063	0.0
21	4.670278348589576E-4	0.0	0.0	19.326779376050812	0.0
22	4.670278348589576E-4	0.0	0.0	20.231412292172614	0.0
23	4.670278348589576E-4	0.0	0.0	21.140014944890716	0.0
24	4.670278348589576E-4	0.0	0.0	21.816037735849058	0.0
25	4.670278348589576E-4	0.0	0.0	22.45586586960583	0.0
26	4.670278348589576E-4	0.0	0.0	23.00579114515225	0.0
27	7.005417522884364E-4	0.0	0.0	23.546842891836352	0.0
28	7.005417522884364E-4	0.0	0.0	24.128759574070614	0.0
29	7.005417522884364E-4	0.0	0.0	24.737997384644125	0.0
30	7.005417522884364E-4	0.0	0.0	25.399542312721838	0.0
31	7.005417522884364E-4	0.0	0.0	26.002708761442182	0.0
32	7.005417522884364E-4	0.0	0.0	26.548430786474874	0.0
33	7.005417522884364E-4	0.0	0.0	27.107463104801045	0.0
34	7.005417522884364E-4	0.0	0.0	27.689379787035307	0.0
35	7.005417522884364E-4	0.0	0.0	28.281337567719035	0.0
36	7.005417522884364E-4	0.0	0.0	28.853913693256118	0.0
37	7.005417522884364E-4	0.0	0.0	29.42298711003176	0.0
38	7.005417522884364E-4	0.0	0.0	29.997431346908275	0.0
39	7.005417522884364E-4	0.0	0.0	30.610638894078086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGT	20	7.028322E-4	45.000004	16
GTCGCAA	20	7.028322E-4	45.000004	13
ATCTATG	20	7.028322E-4	45.000004	26
AATGCGG	35	1.209537E-7	45.000004	2
AGTGCGC	20	7.028322E-4	45.000004	13
CTAACGG	20	7.028322E-4	45.000004	2
GTAGACG	20	7.028322E-4	45.000004	1
TACGAAT	20	7.028322E-4	45.000004	12
ACGTGCC	20	7.028322E-4	45.000004	40
TTGCGTA	20	7.028322E-4	45.000004	14
AGCGATA	20	7.028322E-4	45.000004	34
TCCCGAC	20	7.028322E-4	45.000004	38
CGTAAGA	20	7.028322E-4	45.000004	40
CAACTCC	20	7.028322E-4	45.000004	26
GCCGTAA	20	7.028322E-4	45.000004	38
TGCACGT	20	7.028322E-4	45.000004	21
TTTCGGC	25	3.8866925E-5	45.0	16
CTCGCGC	25	3.8866925E-5	45.0	33
ACATGCG	25	3.8866925E-5	45.0	1
TATAGCG	25	3.8866925E-5	45.0	1
>>END_MODULE