SRR3550149_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2525864
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	10719	0.42436964143754374	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	10349	0.40972118847253847	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	9030	0.3575014331729658	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	6253	0.2475588551085886	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	3718	0.14719715709159323	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2797	0.11073438633275583	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.034894E-4	45.0	44
TCCGCGT	20	7.034894E-4	45.0	11
GCGAATT	20	7.034894E-4	45.0	30
CGTTCGC	20	7.034894E-4	45.0	40
CGGATTA	20	7.034894E-4	45.0	6
TAGATCG	20	7.034894E-4	45.0	20
CCGAACA	20	7.034894E-4	45.0	29
TGGTCGA	20	7.034894E-4	45.0	36
TGCGTAT	20	7.034894E-4	45.0	27
TCCGAAC	25	3.8921386E-5	44.999996	28
AACTCGC	25	3.8921386E-5	44.999996	19
TACGGGA	1525	0.0	43.967213	4
TATGGGA	4785	0.0	41.84953	4
TATGGGC	1210	0.0	41.652893	4
TTACGGG	1775	0.0	41.450703	3
ACGGGAC	675	0.0	41.0	5
TACGCAC	55	6.184564E-11	40.909092	22
AGACACG	380	0.0	40.855263	24
TTATGGG	5725	0.0	40.48035	3
ATGGGCG	680	0.0	40.036762	5