##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2525864
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42478098583297	33.0	31.0	34.0	31.0	34.0
2	32.570328410397394	34.0	31.0	34.0	31.0	34.0
3	32.62603568521504	34.0	31.0	34.0	31.0	34.0
4	36.05407021122277	37.0	35.0	37.0	35.0	37.0
5	31.74036250566143	37.0	35.0	37.0	0.0	37.0
6	33.509894040217524	37.0	35.0	37.0	17.0	37.0
7	35.33401125317911	37.0	35.0	37.0	32.0	37.0
8	35.86912121951142	37.0	35.0	37.0	35.0	37.0
9	37.91192756221238	39.0	38.0	39.0	35.0	39.0
10	37.64042204964321	39.0	37.0	39.0	35.0	39.0
11	37.56100170080416	39.0	37.0	39.0	35.0	39.0
12	37.464835002993034	39.0	37.0	39.0	35.0	39.0
13	37.4647546344538	39.0	37.0	39.0	35.0	39.0
14	38.7084332331432	40.0	38.0	41.0	35.0	41.0
15	38.78428569392493	40.0	38.0	41.0	35.0	41.0
16	38.84018933719314	40.0	38.0	41.0	35.0	41.0
17	38.82717280106926	40.0	38.0	41.0	35.0	41.0
18	38.81243645738646	40.0	38.0	41.0	35.0	41.0
19	38.83265963646499	40.0	38.0	41.0	35.0	41.0
20	38.773513142433636	40.0	38.0	41.0	35.0	41.0
21	38.72356983590566	40.0	38.0	41.0	35.0	41.0
22	38.69604697640094	40.0	38.0	41.0	35.0	41.0
23	38.638840808531256	40.0	38.0	41.0	35.0	41.0
24	38.61515228056617	40.0	38.0	41.0	35.0	41.0
25	38.4824091875097	40.0	38.0	41.0	34.0	41.0
26	38.36647182904543	40.0	38.0	41.0	34.0	41.0
27	38.31060540076584	40.0	37.0	41.0	34.0	41.0
28	38.181681198987754	40.0	37.0	41.0	34.0	41.0
29	38.11402632920854	40.0	37.0	41.0	34.0	41.0
30	38.09570982444027	40.0	37.0	41.0	34.0	41.0
31	37.854105763414026	40.0	37.0	41.0	33.0	41.0
32	37.88731934894357	40.0	37.0	41.0	33.0	41.0
33	37.95115453563612	40.0	37.0	41.0	34.0	41.0
34	37.85754617034013	40.0	37.0	41.0	33.0	41.0
35	37.76191275539776	40.0	37.0	41.0	33.0	41.0
36	37.73594381961974	40.0	37.0	41.0	33.0	41.0
37	37.73989890192029	40.0	37.0	41.0	33.0	41.0
38	37.76994485847219	40.0	37.0	41.0	33.0	41.0
39	37.86374286184846	40.0	37.0	41.0	33.0	41.0
40	37.80301631441756	40.0	37.0	41.0	33.0	41.0
41	37.72916831626723	40.0	36.0	41.0	33.0	41.0
42	37.69286826210754	40.0	36.0	41.0	33.0	41.0
43	37.61366882777537	40.0	36.0	41.0	33.0	41.0
44	37.55373567222939	40.0	36.0	41.0	33.0	41.0
45	37.51254422249179	40.0	36.0	41.0	33.0	41.0
46	37.39180929772941	39.0	35.0	41.0	33.0	41.0
47	37.337240643201696	39.0	35.0	41.0	33.0	41.0
48	37.3316176167838	39.0	35.0	41.0	33.0	41.0
49	37.32098006860227	39.0	35.0	41.0	33.0	41.0
50	37.30334016399933	39.0	35.0	41.0	33.0	41.0
51	36.72343245717109	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	16.0
11	19.0
12	15.0
13	20.0
14	36.0
15	47.0
16	78.0
17	97.0
18	172.0
19	361.0
20	533.0
21	949.0
22	1620.0
23	2620.0
24	4095.0
25	6172.0
26	8701.0
27	11848.0
28	14832.0
29	19779.0
30	26221.0
31	36840.0
32	50900.0
33	76909.0
34	125610.0
35	180361.0
36	213461.0
37	349017.0
38	585680.0
39	807768.0
40	1076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.893631644459084	4.240687542955598	56.38533982827262	16.480340984312694
2	19.128979232452735	4.420467610290974	54.41448945786471	22.036063699391576
3	20.18600368032483	4.2412813991568825	52.991214095454076	22.581500825064214
4	18.916061989085716	4.926235141717844	51.08624217297527	25.071460696221177
5	15.921878612625223	17.202470125074036	44.16754029512278	22.708110967177962
6	20.878915095982997	6.198393896108421	53.10400718328461	19.81868382462397
7	84.14399983530389	1.9942878951519163	8.836501094279027	5.025211175265176
8	85.43706232797965	2.5810970028473426	6.793516990621823	5.188323678551181
9	79.84238264609654	5.686173127294265	8.972019079412034	5.499425147197157
10	34.391321147931954	37.004842699369405	15.198047084086872	13.40578906861177
11	25.29934311586055	25.708034953584196	29.755917183189595	19.236704747365653
12	25.07316308399819	22.424247702964216	32.0088492492074	20.493739963830198
13	23.031960548944834	23.866526463815944	32.74459749218485	20.356915495054366
14	18.765341285199835	27.30515182131738	31.288105772915724	22.64140112056706
15	16.28904010667241	26.8271767601106	34.936718683191174	21.947064450025813
16	18.735450523068543	25.537914947123042	33.86983622237777	21.856798307430644
17	18.552226089765718	25.112238822042677	30.913026196184752	25.422508892006856
18	19.70391121612248	25.334261860496056	31.363881824199563	23.597945099181906
19	22.04627802605366	26.301970335695035	29.907825599478038	21.743926038773267
20	25.71048955921617	26.298288427247073	29.225049329655118	18.766172683881635
21	24.001252640680576	26.642131167790506	28.352437027488413	21.0041791640405
22	21.836132111625965	25.274124022512694	28.5326525893714	24.357091276489946
23	20.839601815457996	26.93965312463379	28.897794972334218	23.322950087573993
24	21.637269465022662	24.91507856321639	29.86225703363285	23.5853949381281
25	21.029437847801784	26.814230694922607	27.51719807558918	24.639133381686428
26	19.68395764775934	29.114987980350488	28.280817969613565	22.920236402276608
27	21.002199643369558	27.294502000107684	29.45312970136159	22.250168655161165
28	18.300391470007888	27.723701671982337	30.946242553043234	23.029664304966538
29	19.632450519901308	25.795648538480297	31.071031536139714	23.50086940547868
30	20.432018509310083	24.858820585748084	31.517136314544253	23.192024590397583
31	19.63387577478439	27.50773596678206	27.857675630992006	25.00071262744154
32	19.687085290419436	27.831941862269705	28.24316748645216	24.2378053608587
33	19.788357567945067	26.119339758593497	28.511709260672784	25.580593412788655
34	19.16916350207295	24.832334599170817	29.38527173276154	26.613230165994683
35	18.252922564318585	24.7936151748471	29.314404892741653	27.63905736809266
36	18.0409159004602	26.591257486547175	29.579502300994825	25.788324311997794
37	18.882370547266202	25.81971950983901	29.990490382696773	25.307419560198014
38	17.960547361219763	25.346614069482758	30.14948548298721	26.54335308631027
39	20.382807625430345	22.810848090000093	30.909106745256278	25.897237539313277
40	20.43094956814777	23.464842129267453	31.28961020862564	24.814598093959138
41	17.165611450181007	25.524968881935052	29.02630545429208	28.283114213591865
42	19.621404794557424	25.024664827559995	27.68917883148103	27.66475154640155
43	20.241905344072364	24.512958734120286	29.090956599405192	26.154179322402154
44	19.937534245707607	24.44593216420203	29.553610170618843	26.062923419471517
45	20.15258937139925	23.045738012814628	29.284276588129842	27.517396027656275
46	19.280254202126482	24.693570200137458	30.070977693177465	25.955197904558595
47	17.57446164955833	24.99402184757374	31.107375535658292	26.324140967209637
48	17.599086886704907	22.597376580845207	31.78548013669778	28.018056395752104
49	18.855251114074235	22.358210893381433	32.58869044414109	26.197847548403242
50	18.156876221364254	22.156774869905902	30.99470121906801	28.691647689661835
51	17.173410761624538	22.155983061637524	29.19654423199349	31.474061944744452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	425.0
1	1184.0
2	1943.0
3	3444.0
4	4945.0
5	3629.5
6	2314.0
7	2425.0
8	2536.0
9	2708.5
10	2881.0
11	3173.0
12	3465.0
13	3589.0
14	3713.0
15	3602.0
16	3491.0
17	4267.0
18	5043.0
19	5757.0
20	6471.0
21	8105.0
22	9739.0
23	11583.5
24	13428.0
25	16384.5
26	25857.0
27	32373.0
28	37401.0
29	42429.0
30	47991.0
31	53553.0
32	59073.5
33	64594.0
34	75394.0
35	86194.0
36	92911.5
37	99629.0
38	106334.5
39	113040.0
40	125273.0
41	137506.0
42	148865.5
43	160225.0
44	174571.0
45	188917.0
46	212829.5
47	236742.0
48	257188.5
49	277635.0
50	260115.0
51	242595.0
52	206716.5
53	170838.0
54	144982.5
55	119127.0
56	108014.0
57	96901.0
58	94283.5
59	91666.0
60	86702.5
61	81739.0
62	69347.5
63	56956.0
64	46012.5
65	35069.0
66	27726.5
67	20384.0
68	17005.0
69	13626.0
70	11704.5
71	9783.0
72	7990.5
73	6198.0
74	5099.5
75	3207.0
76	2413.0
77	1707.0
78	1001.0
79	830.5
80	660.0
81	406.5
82	153.0
83	127.0
84	101.0
85	56.5
86	12.0
87	40.5
88	69.0
89	34.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2525864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.920867964208682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.96743028402375	14.891098915140407
2	13.542337094863367	6.2080424095633715
3	6.142308098811629	4.22361098785053
4	3.5636070301042917	3.2672386485338523
5	2.156472129433122	2.471410647361626
6	1.3620230274477447	1.8731249985804923
7	0.9685444890157352	1.5539916245134142
8	0.6919731301592768	1.2688499800928776
9	0.5081656172460671	1.0482837315162938
>10	4.152175936633415	22.011759189119644
>50	1.6411648532681153	26.432571203695172
>100	0.2909146937394246	10.292473352157604
>500	0.0080289196511283	1.2478076882023115
>1k	0.004107819356391223	1.6539999906007248
>5k	3.73438123308293E-4	0.6540761729591139
>10k+	3.73438123308293E-4	0.9016604601127142
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	10719	0.42436964143754374	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	10349	0.40972118847253847	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	9030	0.3575014331729658	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	6253	0.2475588551085886	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	3718	0.14719715709159323	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2797	0.11073438633275583	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.918082683786617E-5	0.0	0.0	0.4441648481470103	0.0
2	7.918082683786617E-5	0.0	0.0	1.543709400031039	0.0
3	7.918082683786617E-5	0.0	0.0	2.1835696617078355	0.0
4	7.918082683786617E-5	0.0	0.0	2.949525390123934	0.0
5	7.918082683786617E-5	0.0	0.0	4.212895072735508	0.0
6	7.918082683786617E-5	0.0	0.0	5.129413143383808	0.0
7	7.918082683786617E-5	0.0	0.0	5.96132650055585	0.0
8	7.918082683786617E-5	0.0	0.0	7.255576705634191	0.0
9	7.918082683786617E-5	0.0	0.0	7.902919555447165	0.0
10	7.918082683786617E-5	0.0	0.0	9.02637671703623	0.0
11	1.1877124025679926E-4	0.0	0.0	11.066312358860175	0.0
12	1.1877124025679926E-4	0.0	0.0	12.839091890933162	0.0
13	1.1877124025679926E-4	0.0	0.0	13.41691397478249	0.0
14	1.1877124025679926E-4	0.0	0.0	13.623536342415902	0.0
15	1.5836165367573234E-4	0.0	0.0	13.94124941010284	0.0
16	1.5836165367573234E-4	0.0	0.0	14.689033138759648	0.0
17	1.5836165367573234E-4	0.0	0.0	15.803424095675776	0.0
18	1.5836165367573234E-4	0.0	0.0	16.97062866409276	0.0
19	1.5836165367573234E-4	0.0	0.0	17.779817123962335	0.0
20	1.9795206709466543E-4	0.0	0.0	18.60452502589213	0.0
21	2.375424805135985E-4	0.0	0.0	19.690094161839276	0.0
22	2.375424805135985E-4	0.0	0.0	20.912804489869604	0.0
23	2.375424805135985E-4	0.0	0.0	22.04556539861212	0.0
24	2.375424805135985E-4	0.0	0.0	22.85562484757691	0.0
25	2.375424805135985E-4	0.0	0.0	23.558552637830065	0.0
26	2.771328939325316E-4	0.0	0.0	24.212427905857165	0.0
27	2.771328939325316E-4	0.0	0.0	24.84227179293897	0.0
28	2.771328939325316E-4	0.0	0.0	25.528452838315918	0.0
29	2.771328939325316E-4	0.0	0.0	26.259648183750194	0.0
30	2.771328939325316E-4	0.0	0.0	27.060443475974953	0.0
31	2.771328939325316E-4	0.0	0.0	27.824657226200618	0.0
32	3.167233073514647E-4	0.0	0.0	28.528653957616086	0.0
33	3.167233073514647E-4	0.0	0.0	29.211390637025588	0.0
34	3.167233073514647E-4	0.0	0.0	29.857506184022576	0.0
35	3.167233073514647E-4	0.0	0.0	30.54610224461808	0.0
36	3.167233073514647E-4	0.0	0.0	31.280702365606384	0.0
37	3.167233073514647E-4	0.0	0.0	31.964983071139223	0.0
38	3.167233073514647E-4	0.0	0.0	32.63200235642141	0.0
39	3.167233073514647E-4	0.0	0.0	33.31481821665775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.034894E-4	45.0	44
TCCGCGT	20	7.034894E-4	45.0	11
GCGAATT	20	7.034894E-4	45.0	30
CGTTCGC	20	7.034894E-4	45.0	40
CGGATTA	20	7.034894E-4	45.0	6
TAGATCG	20	7.034894E-4	45.0	20
CCGAACA	20	7.034894E-4	45.0	29
TGGTCGA	20	7.034894E-4	45.0	36
TGCGTAT	20	7.034894E-4	45.0	27
TCCGAAC	25	3.8921386E-5	44.999996	28
AACTCGC	25	3.8921386E-5	44.999996	19
TACGGGA	1525	0.0	43.967213	4
TATGGGA	4785	0.0	41.84953	4
TATGGGC	1210	0.0	41.652893	4
TTACGGG	1775	0.0	41.450703	3
ACGGGAC	675	0.0	41.0	5
TACGCAC	55	6.184564E-11	40.909092	22
AGACACG	380	0.0	40.855263	24
TTATGGG	5725	0.0	40.48035	3
ATGGGCG	680	0.0	40.036762	5
>>END_MODULE