##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2520899
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.255608812570436	33.0	31.0	34.0	30.0	34.0
2	32.39999460509921	34.0	31.0	34.0	30.0	34.0
3	32.449810960296304	34.0	31.0	34.0	30.0	34.0
4	35.946002200008806	37.0	35.0	37.0	35.0	37.0
5	31.65158064642812	37.0	35.0	37.0	0.0	37.0
6	33.4389453921002	37.0	35.0	37.0	17.0	37.0
7	35.266603303028006	37.0	35.0	37.0	32.0	37.0
8	35.83052871217768	37.0	35.0	37.0	35.0	37.0
9	37.887358835082246	39.0	38.0	39.0	35.0	39.0
10	37.45724441955033	39.0	37.0	39.0	35.0	39.0
11	37.350110813642274	39.0	37.0	39.0	34.0	39.0
12	37.28920397048831	39.0	37.0	39.0	34.0	39.0
13	37.301945456759675	39.0	37.0	39.0	34.0	39.0
14	38.497140107556866	40.0	38.0	41.0	34.0	41.0
15	38.55413525095611	40.0	38.0	41.0	34.0	41.0
16	38.592869051873954	40.0	38.0	41.0	35.0	41.0
17	38.59921480392511	40.0	38.0	41.0	35.0	41.0
18	38.57832781083256	40.0	38.0	41.0	35.0	41.0
19	38.58767288971117	40.0	38.0	41.0	34.0	41.0
20	38.509441274719855	40.0	38.0	41.0	34.0	41.0
21	38.46335890489861	40.0	38.0	41.0	34.0	41.0
22	38.42194034747128	40.0	38.0	41.0	34.0	41.0
23	38.333260872410996	40.0	38.0	41.0	34.0	41.0
24	38.30152893868418	40.0	37.0	41.0	34.0	41.0
25	38.19321400817724	40.0	37.0	41.0	34.0	41.0
26	38.15681588195322	40.0	37.0	41.0	34.0	41.0
27	38.11593602123687	40.0	37.0	41.0	34.0	41.0
28	38.0898687333368	40.0	37.0	41.0	34.0	41.0
29	38.07539254845196	40.0	37.0	41.0	34.0	41.0
30	38.04098141179	40.0	37.0	41.0	34.0	41.0
31	37.82807522237107	40.0	37.0	41.0	33.0	41.0
32	37.880517228179315	40.0	37.0	41.0	33.0	41.0
33	37.8686928750418	40.0	37.0	41.0	33.0	41.0
34	37.881986941959994	40.0	37.0	41.0	33.0	41.0
35	37.89478872418133	40.0	37.0	41.0	33.0	41.0
36	37.90288305878181	40.0	37.0	41.0	33.0	41.0
37	37.8434526730345	40.0	37.0	41.0	33.0	41.0
38	37.86815536838247	40.0	37.0	41.0	33.0	41.0
39	37.85954217126509	40.0	37.0	41.0	33.0	41.0
40	37.81421112071527	40.0	37.0	41.0	33.0	41.0
41	37.78440310381336	40.0	37.0	41.0	33.0	41.0
42	37.75788994323057	40.0	37.0	41.0	33.0	41.0
43	37.699166051476084	40.0	37.0	41.0	33.0	41.0
44	37.66427889415641	40.0	36.0	41.0	33.0	41.0
45	37.669222765370606	40.0	36.0	41.0	33.0	41.0
46	37.572395403385855	40.0	36.0	41.0	33.0	41.0
47	37.54720082002492	39.0	36.0	41.0	33.0	41.0
48	37.551900730652044	39.0	36.0	41.0	33.0	41.0
49	37.56381354429511	40.0	36.0	41.0	33.0	41.0
50	37.51819410456349	39.0	36.0	41.0	33.0	41.0
51	36.87311431358416	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	6.0
12	10.0
13	25.0
14	21.0
15	42.0
16	42.0
17	77.0
18	154.0
19	274.0
20	561.0
21	896.0
22	1655.0
23	2777.0
24	4380.0
25	6777.0
26	9378.0
27	12112.0
28	15578.0
29	21258.0
30	28844.0
31	39704.0
32	54946.0
33	79658.0
34	124407.0
35	177599.0
36	216072.0
37	346389.0
38	581493.0
39	795138.0
40	614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.78532341041827	4.931772355814335	60.88792133282611	12.394982900941292
2	19.298353484213372	4.174145810681031	54.84563245096293	21.68186825414267
3	18.84323806705465	4.040265000700146	54.38738323114095	22.729113701104247
4	17.64330899413265	4.29533273645632	53.91056920566829	24.150789063742735
5	14.687974409129442	16.78706683607713	47.556090109123765	20.968868645669662
6	19.393795626084188	5.992941407013927	56.99189059141203	17.621372375489855
7	82.26811149514518	1.6408828755138543	10.578884755002084	5.512120874338876
8	84.85572012206757	1.44599208456983	7.767705092508664	5.930582700853941
9	79.99312943517373	4.203738428235324	10.130433627051302	5.672698509539653
10	35.72209755329349	25.408990998846047	21.88469272271519	16.984218725145276
11	24.373685736715352	27.777868133550772	26.070064687240546	21.778381442493334
12	19.754143263970512	23.914563812354245	33.59138942099624	22.739903502679006
13	21.26332709085132	25.70939970224908	34.560012122659415	18.467261084240185
14	20.18815509863743	27.07062044135842	32.18946891565271	20.55175554435144
15	18.103660638526176	27.21227625541523	33.94519177483906	20.73887133121954
16	18.60974993444799	26.071968769871383	31.78017048679856	23.53811080888207
17	18.74612985288185	27.765848611943593	28.93626440408759	24.551757131086966
18	20.10366143189394	26.04297117813923	32.132901794161526	21.720465595805305
19	21.449213157687	27.67215187915105	29.907663892920738	20.970971070241212
20	22.305098300249238	28.628596385654483	29.781756428956495	19.284548885139785
21	22.799128406175733	28.962604213814195	29.111479674512942	19.126787705497126
22	20.462938023300417	26.935827258450256	27.901236820673898	24.69999789757543
23	19.153920882986586	28.052294042720472	27.208031737883985	25.585753336408956
24	20.611932489163586	27.488526910439493	28.533907943158372	23.36563265723855
25	18.268760469975195	29.65569029143968	26.794647465051156	25.280901773533966
26	17.750254968564786	29.189745404318064	29.583731835349212	23.47626779176794
27	19.117981323329495	28.515938163329828	29.23465001969536	23.131430493645322
28	17.055621823801747	27.81380769320786	30.348974711005877	24.78159577198452
29	17.118694560948295	25.48574932990175	30.4496530801115	26.945903029038448
30	18.942012353529435	27.687027524704483	28.251865703465313	25.119094418300776
31	18.046498491212855	29.765135374324796	26.67595964772885	25.512406486733504
32	16.72030493883333	28.718683295126063	27.195298185290245	27.365713580750363
33	17.01619144598812	26.942213868941202	27.48309234126397	28.558502343806712
34	15.631328347545855	27.082322615860456	29.472977695655402	27.813371340938293
35	15.843673229272573	24.680282708668614	29.46115651598894	30.014887546069875
36	17.658382981626794	26.13055897915783	29.6186400169146	26.592418022300773
37	15.244878910261775	29.30680681772653	30.411531759106573	25.03678251290512
38	16.249322166417617	29.220488405128485	26.71562803587133	27.814561392582565
39	17.911665639916556	25.135913814873184	29.915200886667815	27.03721965854245
40	18.51157067379534	24.199779523098705	30.81729970141604	26.47135010168991
41	16.458771255809932	25.14388716088983	29.528870454548162	28.868471128752084
42	17.971247558906565	24.85573598942282	30.325491025225524	26.84752542644509
43	18.257097963861305	25.556676407900515	30.26376701327582	25.922458614962363
44	17.811740970185635	25.40458780776223	27.541880892491132	29.241790329561002
45	18.963234941185664	24.093944263534556	27.258965948258933	29.683854847020847
46	19.271894669322336	24.620899131619314	29.34877597238128	26.75843022667707
47	15.247338350326608	25.62058218119806	32.07494627908536	27.057133189389976
48	15.649377464150685	24.42819010202313	31.126276776657853	28.796155657168338
49	18.41208235633399	22.920553342279877	31.83019232424623	26.8371719771399
50	18.279113919280384	21.2652708418703	29.829199821174907	30.62641541767441
51	16.57242118783815	21.67468827588888	28.15733593452177	33.595554601751196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	332.0
1	634.5
2	937.0
3	2409.0
4	3881.0
5	2785.5
6	1690.0
7	2066.5
8	2443.0
9	3282.5
10	4122.0
11	5035.5
12	5949.0
13	6326.5
14	6704.0
15	7027.0
16	7350.0
17	7155.0
18	6960.0
19	7437.5
20	7915.0
21	8059.5
22	8204.0
23	8743.5
24	9283.0
25	11306.5
26	17002.0
27	20674.0
28	25457.0
29	30240.0
30	35644.5
31	41049.0
32	47364.5
33	53680.0
34	60684.5
35	67689.0
36	75759.0
37	83829.0
38	95392.0
39	106955.0
40	119650.0
41	132345.0
42	153558.5
43	174772.0
44	199400.0
45	224028.0
46	268979.5
47	313931.0
48	347477.0
49	381023.0
50	345693.0
51	310363.0
52	250698.0
53	191033.0
54	152459.0
55	113885.0
56	93613.5
57	73342.0
58	61024.0
59	48706.0
60	40880.5
61	33055.0
62	25848.5
63	18642.0
64	14494.0
65	10346.0
66	7843.5
67	5341.0
68	4104.0
69	2867.0
70	2377.5
71	1888.0
72	1381.5
73	875.0
74	715.0
75	403.0
76	251.0
77	212.5
78	174.0
79	161.5
80	149.0
81	116.5
82	84.0
83	50.5
84	17.0
85	12.5
86	8.0
87	5.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2520899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.45234946811572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.48346177167002	16.792867162299803
2	14.532806097122023	7.688537312668692
3	6.786540208746511	5.385597998435451
4	3.7772112242643106	3.99664445276515
5	2.3119882077265808	3.057876001847302
6	1.495470820724714	2.3735230061507977
7	1.069852633664595	1.9810081021555896
8	0.7600181735077952	1.6083413062197764
9	0.5572675090338688	1.3266931406571207
>10	4.186947467754928	23.625374539225607
>50	0.8040259574693411	14.744671157118
>100	0.21551773052853268	9.774152879116592
>500	0.012167856201766985	2.1299640346068895
>1k	0.0059238247298076105	2.78695669897085
>5k	1.6010337107588135E-4	0.26084044477648644
>10k+	6.404134843035254E-4	2.4669517629859286
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	18267	0.7246224461987569	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	15312	0.6074023592377165	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	14134	0.5606729980058701	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	10537	0.41798580585735484	No Hit
TCCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	6159	0.2443176025695595	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCT	4484	0.17787305243089868	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCC	3626	0.1438375754046473	No Hit
TGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	3289	0.13046932860063018	No Hit
GCCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	2857	0.11333258492307705	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	2857	0.11333258492307705	No Hit
TGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCTT	2826	0.11210286489066004	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	2679	0.10627161183371486	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9834194071242044E-4	0.0	0.0	0.7495341939522369	0.0
2	1.9834194071242044E-4	0.0	0.0	2.438653829447352	0.0
3	1.9834194071242044E-4	0.0	0.0	3.4180266642971415	0.0
4	1.9834194071242044E-4	0.0	0.0	4.559722543425976	0.0
5	1.9834194071242044E-4	0.0	0.0	6.458330936701549	0.0
6	1.9834194071242044E-4	0.0	0.0	7.788372322730899	0.0
7	1.9834194071242044E-4	0.0	0.0	8.910789365222486	0.0
8	1.9834194071242044E-4	0.0	0.0	10.745809332305658	0.0
9	1.9834194071242044E-4	0.0	0.0	11.542072887489741	0.0
10	2.3801032885490455E-4	0.0	0.0	13.160939807584516	0.0
11	2.3801032885490455E-4	0.0	0.0	16.299264667089002	0.0
12	2.3801032885490455E-4	0.0	0.0	18.730540176341854	0.0
13	2.3801032885490455E-4	0.0	0.0	19.510698365940087	0.0
14	2.3801032885490455E-4	0.0	0.0	19.757713418903336	0.0
15	2.3801032885490455E-4	0.0	0.0	20.104216789327936	0.0
16	2.3801032885490455E-4	0.0	0.0	21.09330837927263	0.0
17	2.3801032885490455E-4	0.0	0.0	22.67163420668579	0.0
18	2.3801032885490455E-4	0.0	0.0	24.345917865015615	0.0
19	2.3801032885490455E-4	0.0	0.0	25.545529590832476	0.0
20	2.3801032885490455E-4	0.0	0.0	26.639464730637762	0.0
21	2.3801032885490455E-4	0.0	0.0	28.235681001103178	0.0
22	2.776787169973886E-4	0.0	0.0	29.90750521936817	0.0
23	2.776787169973886E-4	0.0	0.0	31.581273188652144	0.0
24	2.776787169973886E-4	0.0	0.0	32.829875373824976	0.0
25	2.776787169973886E-4	0.0	0.0	33.84463241089786	0.0
26	2.776787169973886E-4	0.0	0.0	34.7136874583234	0.0
27	2.776787169973886E-4	0.0	0.0	35.64740197842119	0.0
28	2.776787169973886E-4	0.0	0.0	36.59745987443368	0.0
29	2.776787169973886E-4	0.0	0.0	37.606584000390335	0.0
30	2.776787169973886E-4	0.0	0.0	38.63300354357711	0.0
31	2.776787169973886E-4	0.0	0.0	39.61967536184512	0.0
32	2.776787169973886E-4	0.0	0.0	40.538077884119915	0.0
33	2.776787169973886E-4	0.0	0.0	41.43966100982229	0.0
34	2.776787169973886E-4	0.0	0.0	42.30284513580274	0.0
35	2.776787169973886E-4	0.0	0.0	43.18911626368212	0.0
36	2.776787169973886E-4	0.0	0.0	44.06447858482232	0.0
37	2.776787169973886E-4	0.0	0.0	44.91945135445728	0.0
38	3.173471051398727E-4	0.0	0.0	45.77739925320292	0.0
39	3.173471051398727E-4	0.0	0.0	46.61666334113346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	45	3.8562575E-10	45.000004	13
CGAACGA	55	1.8189894E-12	45.000004	22
GGTCGAA	25	3.8921346E-5	45.000004	25
CGACGGT	50	2.1827873E-11	45.000004	28
TCCGATA	55	1.8189894E-12	45.000004	14
CCGATAA	55	1.8189894E-12	45.000004	15
CGCGATA	25	3.8921346E-5	45.000004	40
CGCGACT	25	3.8921346E-5	45.000004	24
AACGCCG	25	3.8921346E-5	45.000004	36
ACTACGG	30	2.1665364E-6	45.000004	2
CCCGCGA	25	3.8921346E-5	45.000004	38
TCCGCGT	20	7.034891E-4	45.0	26
CGACGAA	20	7.034891E-4	45.0	38
CCGGCAC	20	7.034891E-4	45.0	30
CTACGCC	20	7.034891E-4	45.0	44
ACTACGT	20	7.034891E-4	45.0	35
ACGTCGT	20	7.034891E-4	45.0	1
CCGCGTT	20	7.034891E-4	45.0	25
TTCGATC	20	7.034891E-4	45.0	1
GCCCGAC	80	0.0	45.0	9
>>END_MODULE