FastQCFastQC Report
Sat 18 Jun 2016
SRR3550147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064322
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC32660.30686202108008664No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT30110.2829031063907351No Hit
TCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC24310.2284083200384846No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC23480.22060992819842115No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG19060.1790811427368785No Hit
TTTCTTGGGACTCAGTCTCTGTCTGTCTATCTCTGTGGCGTTCAGCCCCAC10880.10222470267456654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG302.1653486E-645.00000415
ATCACGA351.2118107E-745.00000444
CGTAAGT302.1653486E-645.00000420
CCAGTCG302.1653486E-645.00000428
TCGAATC302.1653486E-645.00000445
TGCCGAT302.1653486E-645.00000420
GTCGCGA207.0330524E-445.017
GTCGACT207.0330524E-445.042
GCGCCTA207.0330524E-445.015
CGCTATA207.0330524E-445.036
TCGCTAA207.0330524E-445.020
AACGCCA207.0330524E-445.034
ACTACGG207.0330524E-445.02
TCCGTGA207.0330524E-445.026
ATAGTCG207.0330524E-445.038
CGGTATC207.0330524E-445.028
CGGTATA406.8139343E-945.034
CGATCAA207.0330524E-445.021
ACGACCG207.0330524E-445.032
AGTCGTT406.8139343E-945.026