##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1064322
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.480375299956215	34.0	31.0	34.0	31.0	34.0
2	32.6248531929247	34.0	31.0	34.0	31.0	34.0
3	32.68389171698039	34.0	31.0	34.0	31.0	34.0
4	36.07157608317784	37.0	35.0	37.0	35.0	37.0
5	31.71157412888205	37.0	35.0	37.0	0.0	37.0
6	33.482696026202596	37.0	35.0	37.0	17.0	37.0
7	35.25614334759594	37.0	35.0	37.0	32.0	37.0
8	35.79846700528599	37.0	35.0	37.0	35.0	37.0
9	37.85448858522139	39.0	38.0	39.0	35.0	39.0
10	37.67487001114324	39.0	37.0	39.0	35.0	39.0
11	37.59373291165643	39.0	37.0	39.0	35.0	39.0
12	37.35007544709214	39.0	37.0	39.0	35.0	39.0
13	37.28725517277666	39.0	37.0	39.0	34.0	39.0
14	38.551361336137	40.0	38.0	41.0	35.0	41.0
15	38.652083673925745	40.0	38.0	41.0	35.0	41.0
16	38.69927803803736	40.0	38.0	41.0	35.0	41.0
17	38.68413882264954	40.0	38.0	41.0	35.0	41.0
18	38.67105349696802	40.0	38.0	41.0	35.0	41.0
19	38.68573326493298	40.0	38.0	41.0	35.0	41.0
20	38.60527453157973	40.0	38.0	41.0	35.0	41.0
21	38.55809332138206	40.0	38.0	41.0	35.0	41.0
22	38.50766873183116	40.0	38.0	41.0	34.0	41.0
23	38.48034147560607	40.0	38.0	41.0	34.0	41.0
24	38.46349600966625	40.0	37.0	41.0	34.0	41.0
25	38.370857691563266	40.0	37.0	41.0	34.0	41.0
26	38.315753127343044	40.0	37.0	41.0	34.0	41.0
27	38.25004368978561	40.0	37.0	41.0	34.0	41.0
28	38.22061274689427	40.0	37.0	41.0	34.0	41.0
29	38.15702766643929	40.0	37.0	41.0	34.0	41.0
30	38.112004637694234	40.0	37.0	41.0	34.0	41.0
31	37.924647803954066	40.0	36.0	41.0	34.0	41.0
32	37.94376983657202	40.0	36.0	41.0	34.0	41.0
33	37.9392768353938	40.0	36.0	41.0	34.0	41.0
34	37.87147310682293	40.0	36.0	41.0	34.0	41.0
35	37.76283681066444	40.0	36.0	41.0	33.0	41.0
36	37.73113024066025	40.0	36.0	41.0	33.0	41.0
37	37.69056826787382	40.0	36.0	41.0	33.0	41.0
38	37.648281253229754	40.0	35.0	41.0	33.0	41.0
39	37.638955128241264	40.0	35.0	41.0	33.0	41.0
40	37.53238305700718	40.0	35.0	41.0	33.0	41.0
41	37.4874182813096	40.0	35.0	41.0	33.0	41.0
42	37.446205189782795	39.0	35.0	41.0	33.0	41.0
43	37.39513605844848	39.0	35.0	41.0	33.0	41.0
44	37.31940709672449	39.0	35.0	41.0	33.0	41.0
45	37.30576742752663	39.0	35.0	41.0	33.0	41.0
46	37.23968122429115	39.0	35.0	41.0	33.0	41.0
47	37.19443645813955	39.0	35.0	41.0	33.0	41.0
48	37.13766698423973	39.0	35.0	41.0	33.0	41.0
49	37.06533736970579	39.0	35.0	41.0	33.0	41.0
50	36.96989820749736	39.0	35.0	41.0	33.0	41.0
51	36.32966621003794	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	14.0
11	9.0
12	17.0
13	17.0
14	23.0
15	29.0
16	42.0
17	67.0
18	124.0
19	204.0
20	363.0
21	638.0
22	950.0
23	1482.0
24	2094.0
25	3184.0
26	4359.0
27	5576.0
28	7095.0
29	8848.0
30	11559.0
31	15842.0
32	21727.0
33	33893.0
34	59311.0
35	92543.0
36	83775.0
37	135609.0
38	214618.0
39	359834.0
40	469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.265776710431616	4.826640809830108	53.91460479065546	16.992977689082814
2	19.5337501244924	5.010419778976663	53.148201390180795	22.307628706350147
3	21.509280086289674	5.104658176754779	51.15444386191397	22.231617875041575
4	18.975366477438218	5.312302104062493	50.67310456797849	25.0392268505208
5	16.805158589223936	17.283773143841806	43.176031313831714	22.73503695310254
6	21.146419974406243	6.981439827420649	53.093142864659384	18.77899733351373
7	84.31874940102713	2.6858413149404035	8.178727866190872	4.816681417841593
8	85.91582246726085	3.6814986442072986	5.963045018330919	4.439633870200935
9	81.12526096425707	5.559501729739684	7.955862981315804	5.359374324687454
10	39.79481773373096	29.473035415973737	15.63906411781397	15.093082732481337
11	31.322663629991677	26.241776454869857	22.669549252951647	19.76601066218682
12	28.454828519940396	22.68589768885732	28.26419072423571	20.595083066966577
13	25.33293495765379	24.261266797078328	29.366394756474072	21.039403488793802
14	21.088918579151798	27.493841149576916	28.294444726314026	23.12279554495726
15	19.76817166233527	24.966786367283586	34.01940390220253	21.245638068178614
16	21.76878801716022	23.5538680963092	32.16404433996479	22.513299546565797
17	21.805055237042925	23.829536550029033	28.226983939071072	26.13842427385697
18	21.913011287937298	24.342351280909348	29.4648611980209	24.279776233132456
19	24.42663028669895	25.101238159128535	27.587703721242256	22.88442783293026
20	27.256319046303656	25.431119529616037	27.39800549081951	19.9145559332608
21	27.219300174195403	24.692245391902073	27.064459815732455	21.023994618170065
22	24.25600523149949	23.98146425611798	27.09029786098568	24.672232651396854
23	23.798155069612392	25.766450378738764	26.54130986675085	23.894084684897994
24	23.98127634306159	24.774269441015033	28.278378159992933	22.966076055930444
25	23.449106567373406	25.83362929639714	26.906612848367317	23.810651287862132
26	21.41767247130098	27.9634358774882	26.480801862594216	24.138089788616604
27	22.387303842258262	27.295780788144942	27.678747597061793	22.638167772535002
28	21.908595331112203	26.88829132536958	28.681451665943204	22.52166167757502
29	21.36665407649189	25.588966496981175	28.931939770107167	24.112439656419767
30	22.696326863486803	27.64445346427115	26.566302303250332	23.092917368991714
31	24.58400747142312	28.105592104645023	24.290769146931098	23.01963127700076
32	24.14419696294918	28.153791803608307	25.33368660987934	22.368324623563172
33	25.04298511164854	27.76189912451307	24.523029684625516	22.672086079212868
34	23.359565996004967	25.817562730076048	27.02546785653214	23.797403417386846
35	22.409571539440133	25.088647984350597	27.481532844383562	25.02024763182571
36	24.235898534466074	28.418655256585883	25.632468369534784	21.712977839413263
37	24.10163465567751	28.8326277198066	25.573369713301048	21.49236791121484
38	22.87005248411665	27.883760741580087	24.739693438639808	24.506493335663453
39	23.546727400166493	26.443782990485964	26.37387933350997	23.635610275837575
40	23.635234449724802	26.59467717476478	27.71877307807224	22.051315297438183
41	21.456288604388522	26.72273992269257	27.02856842196253	24.792403050956384
42	22.724889648057637	26.327370852054173	26.261319412734117	24.686420087154076
43	23.22304716053976	25.757148682447607	26.349544592707847	24.670259564304786
44	22.790565261264916	25.196416122188587	27.435024362927763	24.577994253618733
45	22.654704121497065	26.107230706496722	25.44925313955739	25.788812032448828
46	23.40194039022025	27.366905879987446	26.231911019409537	22.99924271038276
47	21.863684110635692	26.50617012520647	28.29688759604706	23.333258168110778
48	21.90089089580033	25.230428385394642	27.84645999988725	25.02222071891777
49	23.209235550895315	24.962558323514877	28.28401555168455	23.544190573905265
50	22.387397798786456	25.186550686728264	27.502767019755296	24.923284494729977
51	21.943735072656583	25.425106311811653	25.503841882437833	27.12731673309393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	708.0
2	1146.0
3	1519.5
4	1893.0
5	1457.0
6	1021.0
7	1145.5
8	1270.0
9	1288.0
10	1306.0
11	1413.5
12	1521.0
13	1516.5
14	1512.0
15	1650.0
16	1788.0
17	1650.5
18	1513.0
19	1591.5
20	1670.0
21	2072.5
22	2475.0
23	3744.5
24	5014.0
25	5123.0
26	8243.5
27	11255.0
28	12446.5
29	13638.0
30	15737.0
31	17836.0
32	20345.5
33	22855.0
34	25125.5
35	27396.0
36	31214.0
37	35032.0
38	37826.5
39	40621.0
40	47916.0
41	55211.0
42	58008.0
43	60805.0
44	66680.5
45	72556.0
46	80984.0
47	89412.0
48	94178.5
49	98945.0
50	96205.0
51	93465.0
52	83909.5
53	74354.0
54	66515.5
55	58677.0
56	56136.0
57	53595.0
58	52474.0
59	51353.0
60	48330.5
61	45308.0
62	41208.0
63	37108.0
64	31796.5
65	26485.0
66	21559.0
67	16633.0
68	13988.5
69	11344.0
70	10216.5
71	9089.0
72	7754.5
73	6420.0
74	5074.0
75	2857.0
76	1986.0
77	1368.5
78	751.0
79	585.5
80	420.0
81	371.0
82	322.0
83	201.5
84	81.0
85	44.5
86	8.0
87	4.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1064322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.415483481199978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7646728335325	15.638088714713689
2	11.570643695946352	5.187231452666722
3	4.663582066208772	3.136093403049696
4	2.4973225771673846	2.2391477190289306
5	1.5267600048487413	1.7111531834221883
6	1.0664680132026343	1.4343237679903083
7	0.8280076931080556	1.2992134937019084
8	0.6351718507765058	1.1390147303043188
9	0.5454415796077192	1.100370304589081
>10	4.655448308173815	23.363051047034027
>50	1.8924440632483206	30.784794506128364
>100	0.3455472107216756	10.732043919357968
>500	0.0055185672473977675	0.8017750770449106
>1k	0.0029715362101372595	1.433698680967917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	3266	0.30686202108008664	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	3011	0.2829031063907351	No Hit
TCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2431	0.2284083200384846	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2348	0.22060992819842115	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1906	0.1790811427368785	No Hit
TTTCTTGGGACTCAGTCTCTGTCTGTCTATCTCTGTGGCGTTCAGCCCCAC	1088	0.10222470267456654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2973724117325396	0.0
2	0.0	0.0	0.0	1.1119755111704916	0.0
3	0.0	0.0	0.0	1.5351557141541752	0.0
4	0.0	0.0	0.0	1.9602150477017293	0.0
5	0.0	0.0	0.0	2.72004149120285	0.0
6	0.0	0.0	0.0	3.2479832231223256	0.0
7	0.0	0.0	0.0	3.7125042985111647	0.0
8	0.0	0.0	0.0	4.45119052316874	0.0
9	0.0	0.0	0.0	4.7934741553777895	0.0
10	0.0	0.0	0.0	5.495047551398919	0.0
11	0.0	0.0	0.0	6.685006980970045	0.0
12	0.0	0.0	0.0	7.789278056828667	0.0
13	0.0	0.0	0.0	8.152795864409455	0.0
14	0.0	0.0	0.0	8.285556438746921	0.0
15	0.0	0.0	0.0	8.500341062197343	0.0
16	0.0	0.0	0.0	9.001693096638048	0.0
17	0.0	0.0	0.0	9.6956560138755	0.0
18	0.0	0.0	0.0	10.461119849068233	0.0
19	0.0	0.0	0.0	10.964163101016421	0.0
20	0.0	0.0	0.0	11.460629395991063	0.0
21	0.0	0.0	0.0	12.128284485334326	0.0
22	0.0	0.0	0.0	12.849494795747903	0.0
23	0.0	0.0	0.0	13.518089450373102	0.0
24	0.0	0.0	0.0	14.056178487337478	0.0
25	9.395652819353541E-5	0.0	0.0	14.521169345367285	0.0
26	9.395652819353541E-5	0.0	0.0	14.95102046185271	0.0
27	9.395652819353541E-5	0.0	0.0	15.3770193606822	0.0
28	9.395652819353541E-5	0.0	0.0	15.814386999423107	0.0
29	9.395652819353541E-5	0.0	0.0	16.278062466058206	0.0
30	9.395652819353541E-5	0.0	0.0	16.788528283733683	0.0
31	1.8791305638707082E-4	0.0	0.0	17.250042750220327	0.0
32	1.8791305638707082E-4	0.0	0.0	17.742374957954453	0.0
33	3.7582611277414164E-4	0.0	0.0	18.212815294619485	0.0
34	4.6978264096767707E-4	0.0	0.0	18.64670654181723	0.0
35	5.637391691612124E-4	0.0	0.0	19.11461005222104	0.0
36	5.637391691612124E-4	0.0	0.0	19.57603056217949	0.0
37	5.637391691612124E-4	0.0	0.0	20.030686202108008	0.0
38	5.637391691612124E-4	0.0	0.0	20.478107189365623	0.0
39	5.637391691612124E-4	0.0	0.0	20.92101826326995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	30	2.1653486E-6	45.000004	15
ATCACGA	35	1.2118107E-7	45.000004	44
CGTAAGT	30	2.1653486E-6	45.000004	20
CCAGTCG	30	2.1653486E-6	45.000004	28
TCGAATC	30	2.1653486E-6	45.000004	45
TGCCGAT	30	2.1653486E-6	45.000004	20
GTCGCGA	20	7.0330524E-4	45.0	17
GTCGACT	20	7.0330524E-4	45.0	42
GCGCCTA	20	7.0330524E-4	45.0	15
CGCTATA	20	7.0330524E-4	45.0	36
TCGCTAA	20	7.0330524E-4	45.0	20
AACGCCA	20	7.0330524E-4	45.0	34
ACTACGG	20	7.0330524E-4	45.0	2
TCCGTGA	20	7.0330524E-4	45.0	26
ATAGTCG	20	7.0330524E-4	45.0	38
CGGTATC	20	7.0330524E-4	45.0	28
CGGTATA	40	6.8139343E-9	45.0	34
CGATCAA	20	7.0330524E-4	45.0	21
ACGACCG	20	7.0330524E-4	45.0	32
AGTCGTT	40	6.8139343E-9	45.0	26
>>END_MODULE