FastQCFastQC Report
Sat 18 Jun 2016
SRR3550140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469285
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT53530.21678340086300285TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC50860.2059705542292607TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC50660.2051606031705534TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC46290.1874631725377994TruSeq Adapter, Index 16 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG39280.15907438793010933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC351.2126839E-745.031
TACGGGA8650.042.658964
TTACGGG14250.042.631583
TTATGGG48400.041.885333
TATGGGA30400.041.225334
TCGATGG2550.040.5882342
CGTTCAT3450.040.43478417
TATGGGC16950.040.353984
CGATAAC1900.040.26315716
TATGGGT9200.039.8641324
ATGGGCG8800.039.630685
TTTGGGC54850.039.462174
TTTACGG7900.039.3037952
TCGGCGT6650.038.909774
TTTATGG30350.038.7726552
CTATGGG25150.038.6481133
GCGATCG356.2512063E-638.5714269
TACGGGG12300.038.231714
TCCGATA1950.038.07692714
GTACGGG5000.037.83
Produced by FastQC (version 0.11.5)