##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2469285
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43260700972144	33.0	31.0	34.0	31.0	34.0
2	32.58059033282914	34.0	31.0	34.0	31.0	34.0
3	32.637272327819595	34.0	31.0	34.0	31.0	34.0
4	36.0331059395736	37.0	35.0	37.0	35.0	37.0
5	31.806354066055558	37.0	35.0	37.0	0.0	37.0
6	33.49483919434168	37.0	35.0	37.0	17.0	37.0
7	35.26501234162926	37.0	35.0	37.0	32.0	37.0
8	35.806902807897835	37.0	35.0	37.0	35.0	37.0
9	37.83404750767935	39.0	38.0	39.0	35.0	39.0
10	37.61335447305596	39.0	37.0	39.0	35.0	39.0
11	37.55015561184715	39.0	37.0	39.0	35.0	39.0
12	37.39245652081473	39.0	37.0	39.0	35.0	39.0
13	37.37489030225348	39.0	37.0	39.0	35.0	39.0
14	38.63435488410613	40.0	38.0	41.0	35.0	41.0
15	38.71805320163529	40.0	38.0	41.0	35.0	41.0
16	38.756000218686786	40.0	38.0	41.0	35.0	41.0
17	38.74937319912444	40.0	38.0	41.0	35.0	41.0
18	38.702117819530756	40.0	38.0	41.0	35.0	41.0
19	38.72081068001466	40.0	38.0	41.0	35.0	41.0
20	38.661242829402035	40.0	38.0	41.0	35.0	41.0
21	38.603683252439474	40.0	38.0	41.0	35.0	41.0
22	38.55914687854986	40.0	38.0	41.0	34.0	41.0
23	38.50168895044517	40.0	38.0	41.0	34.0	41.0
24	38.4776629672152	40.0	38.0	41.0	34.0	41.0
25	38.384952324255806	40.0	37.0	41.0	34.0	41.0
26	38.32762236841839	40.0	37.0	41.0	34.0	41.0
27	38.28204480244281	40.0	37.0	41.0	34.0	41.0
28	38.227156444071866	40.0	37.0	41.0	34.0	41.0
29	38.157578813300205	40.0	37.0	41.0	34.0	41.0
30	38.109048165764584	40.0	37.0	41.0	34.0	41.0
31	37.91007437375597	40.0	37.0	41.0	34.0	41.0
32	37.94455237042302	40.0	37.0	41.0	34.0	41.0
33	37.94179894179894	40.0	37.0	41.0	34.0	41.0
34	37.881283853423156	40.0	37.0	41.0	33.0	41.0
35	37.78966299961325	40.0	36.0	41.0	33.0	41.0
36	37.791199071796086	40.0	36.0	41.0	33.0	41.0
37	37.771074217840386	40.0	36.0	41.0	33.0	41.0
38	37.74833079211189	40.0	36.0	41.0	33.0	41.0
39	37.71305134887224	40.0	36.0	41.0	33.0	41.0
40	37.59521723899833	40.0	36.0	41.0	33.0	41.0
41	37.52683874076909	40.0	36.0	41.0	33.0	41.0
42	37.51835126362489	40.0	35.0	41.0	33.0	41.0
43	37.48727911115971	40.0	35.0	41.0	33.0	41.0
44	37.432898187127044	39.0	35.0	41.0	33.0	41.0
45	37.39727775449168	39.0	35.0	41.0	33.0	41.0
46	37.2918524998127	39.0	35.0	41.0	33.0	41.0
47	37.25208633268335	39.0	35.0	41.0	33.0	41.0
48	37.2063091137718	39.0	35.0	41.0	33.0	41.0
49	37.16140016239519	39.0	35.0	41.0	33.0	41.0
50	37.08459655325327	39.0	35.0	41.0	33.0	41.0
51	36.443660816795145	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	26.0
11	35.0
12	42.0
13	41.0
14	64.0
15	76.0
16	100.0
17	192.0
18	293.0
19	538.0
20	972.0
21	1693.0
22	2495.0
23	3719.0
24	5468.0
25	7965.0
26	10744.0
27	13614.0
28	16880.0
29	21348.0
30	27582.0
31	36645.0
32	49637.0
33	75547.0
34	129109.0
35	182961.0
36	197882.0
37	316396.0
38	515708.0
39	850427.0
40	1073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.859908840008345	4.711566303606104	53.463127990491174	16.965396865894377
2	20.043008401217357	4.685890855045084	52.33559512166477	22.935505622072785
3	21.98725542009124	4.873151539818207	50.27532261363107	22.86427042645948
4	19.342279242776755	5.100747787314951	49.28629137584362	26.270681594064676
5	16.250615056585204	17.542325005011573	42.88496467600945	23.32209526239377
6	22.123570183271678	7.319001249349507	51.83322297750158	18.724205589877233
7	83.88776508179492	2.957090817787335	8.343265358190731	4.811878742227001
8	85.43841638369003	3.3235126767465073	6.48948987257445	4.748581066989027
9	79.96857389892216	5.742391016022856	8.626707731185343	5.662327353869642
10	38.01282557501463	27.755726860204472	17.21939751790498	17.012050046875917
11	29.129970821513112	26.659377107138297	24.326596565402536	19.88405550594605
12	26.664520296361093	23.08992279141533	29.45901343911294	20.786543473110637
13	24.863634614878396	23.25106255454514	30.160876529035736	21.72442630154073
14	20.610662600712352	26.24010594159848	29.831671921224164	23.31755953646501
15	19.1224585254436	25.692174050383006	32.74988508819355	22.43548233597985
16	21.404819613774837	24.20664281360799	31.987235171314772	22.4013024013024
17	21.473584458659086	23.592821403766678	29.742536807213426	25.19105733036081
18	22.219103910646197	24.2474238494139	29.955675428312244	23.577796811627657
19	24.54257001520683	25.957149539239094	27.784439625235645	21.715840820318434
20	27.52128652626165	25.201424703912267	27.29462172248242	19.982667047343664
21	26.39808689559933	25.45429142444068	26.557363771294117	21.59025790866587
22	24.299787185359325	24.852173807397687	26.964850148929752	23.883188858313236
23	23.58504587360309	25.836750314362256	26.891347090352063	23.68685672168259
24	23.603472260188678	24.99399623777733	28.27324508916549	23.129286412868503
25	23.562205253747543	25.835899865750612	26.25237670013789	24.34951818036395
26	21.96222793237719	27.694980530801427	26.340094399795895	24.002697137025493
27	22.49655264580638	26.76993542665184	26.95152645401402	23.78198547352776
28	20.533393269711677	26.975379512692943	29.09886870085875	23.392358516736625
29	22.230038249938747	26.316200843564026	27.7656082631207	23.688152643376522
30	23.247863247863247	25.653903862859085	27.258740890581684	23.839491998695976
31	22.06650913118575	28.174228572238526	25.6920525577242	24.06720973885153
32	22.900515736336633	28.41567498283916	25.577687468234732	23.106121812589475
33	23.373324666857005	27.230109120656383	25.13002751808722	24.266538694399394
34	21.755447427089216	26.482686283681307	27.02932225320285	24.732544036026624
35	21.29515224042587	26.940268134297984	25.83156662758653	25.933012997689612
36	21.929586904711282	27.934402063755297	25.29938828446291	24.836622747070507
37	21.692635722486468	27.605723924131887	26.994494357678438	23.70714599570321
38	21.84757936001717	27.824694192853393	26.11213367432273	24.215592772806705
39	23.398838125206282	25.469518504344375	26.653221479092124	24.478421891357215
40	22.889743387255827	24.8929548432036	28.539556997268438	23.677744772272135
41	20.235007299683915	26.96310875415353	27.185642807533355	25.6162411386292
42	20.710367576039218	27.15077441445601	26.783988077520416	25.354869931984357
43	21.709604197166385	25.96905581980209	27.498243418641426	24.823096564390095
44	22.487926667031143	25.40666630218869	27.44502963408436	24.660377396695804
45	22.017264106816345	24.8849363277224	26.445509530086646	26.652290035374616
46	21.321273162069183	26.715547213059647	26.954401780272423	25.00877784459874
47	20.053983238062838	26.926215483429417	28.04447441263362	24.975326865874127
48	20.220630668391863	25.076975723741896	28.48330589624122	26.21908771162503
49	21.072618187046047	24.69062906873852	29.083803611166797	25.152949133048637
50	20.829875854751474	24.490125684155533	28.01612612557886	26.663872335514128
51	20.157454485812696	25.415778251599146	26.128332695496876	28.298434567091284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	496.0
1	1414.0
2	2332.0
3	3849.5
4	5367.0
5	4149.5
6	2932.0
7	2932.5
8	2933.0
9	3072.0
10	3211.0
11	3298.5
12	3386.0
13	3437.5
14	3489.0
15	3722.0
16	3955.0
17	4112.5
18	4270.0
19	4432.5
20	4595.0
21	5528.0
22	6461.0
23	8710.5
24	10960.0
25	12605.0
26	18852.5
27	23455.0
28	27645.5
29	31836.0
30	38525.5
31	45215.0
32	49190.0
33	53165.0
34	61124.5
35	69084.0
36	75571.5
37	82059.0
38	89940.0
39	97821.0
40	108115.0
41	118409.0
42	127879.0
43	137349.0
44	151014.0
45	164679.0
46	183186.5
47	201694.0
48	220358.5
49	239023.0
50	232269.5
51	225516.0
52	201548.0
53	177580.0
54	158960.0
55	140340.0
56	130820.5
57	121301.0
58	118815.0
59	116329.0
60	109363.0
61	102397.0
62	89895.5
63	77394.0
64	67775.0
65	58156.0
66	47800.5
67	37445.0
68	32662.5
69	27880.0
70	24209.5
71	20539.0
72	17237.0
73	13935.0
74	11409.0
75	6882.5
76	4882.0
77	3552.5
78	2223.0
79	1615.0
80	1007.0
81	852.5
82	698.0
83	428.5
84	159.0
85	167.0
86	175.0
87	94.0
88	13.0
89	8.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2469285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.428101894364314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50070356314185	16.282695648072963
2	12.637325172197588	5.921370836129202
3	4.999618562356043	3.5139471933549786
4	2.5103344148852846	2.3524948184344745
5	1.4631914038716651	1.7139898650431666
6	0.9201294065144827	1.2934131295093358
7	0.6622196717755156	1.0860184962766488
8	0.4578860828171818	0.8581921443401817
9	0.3482560733852739	0.7343080895341273
>10	4.3865240864964905	26.659495972102974
>50	1.9461541583450421	31.0261823086753
>100	0.15866581970920432	5.896168223452741
>500	0.005994389669613188	0.9147287423308503
>1k	0.0024352208032803573	1.0665325479117365
>5k	5.619740315262363E-4	0.6804619848313503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	5353	0.21678340086300285	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	5086	0.2059705542292607	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	5066	0.2051606031705534	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	4629	0.1874631725377994	TruSeq Adapter, Index 16 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3928	0.15907438793010933	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2149265880609164E-4	0.0	0.0	0.22488291145007563	0.0
2	1.2149265880609164E-4	0.0	0.0	0.8631243457114104	0.0
3	1.2149265880609164E-4	0.0	0.0	1.2642526075361897	0.0
4	1.2149265880609164E-4	0.0	0.0	1.7231708773997332	0.0
5	1.6199021174145552E-4	0.0	0.0	2.5438942851878177	0.0
6	1.6199021174145552E-4	0.0	0.0	3.2209728727141664	0.0
7	1.6199021174145552E-4	0.0	0.0	3.7890320477385155	0.0
8	1.6199021174145552E-4	0.0	0.0	4.654262266202565	0.0
9	1.6199021174145552E-4	0.0	0.0	5.08730259974041	0.0
10	1.6199021174145552E-4	0.0	0.0	5.866435020663876	0.0
11	1.6199021174145552E-4	0.0	0.0	7.20734949590671	0.0
12	1.6199021174145552E-4	0.0	0.0	8.341280978096899	0.0
13	1.6199021174145552E-4	0.0	0.0	8.70814831013836	0.0
14	1.6199021174145552E-4	0.0	0.0	8.849201287012232	0.0
15	2.024877646768194E-4	0.0	0.0	9.065458219687075	0.0
16	2.024877646768194E-4	0.0	0.0	9.60063338172791	0.0
17	2.024877646768194E-4	0.0	0.0	10.343318005009547	0.0
18	2.024877646768194E-4	0.0	0.0	11.138568452001287	0.0
19	2.024877646768194E-4	0.0	0.0	11.65507424213892	0.0
20	2.024877646768194E-4	0.0	0.0	12.195190105637867	0.0
21	2.024877646768194E-4	0.0	0.0	12.955612657105194	0.0
22	2.024877646768194E-4	0.0	0.0	13.775607108940441	0.0
23	2.4298531761218328E-4	0.0	0.0	14.555630476028487	0.0
24	2.4298531761218328E-4	0.0	0.0	15.173623133822138	0.0
25	2.4298531761218328E-4	0.0	0.0	15.710580188192129	0.0
26	2.834828705475472E-4	0.0	0.0	16.194809428640273	0.0
27	3.2398042348291104E-4	0.0	0.0	16.663082633231888	0.0
28	3.2398042348291104E-4	0.0	0.0	17.16027109061935	0.0
29	3.2398042348291104E-4	0.0	0.0	17.70192586112984	0.0
30	3.2398042348291104E-4	0.0	0.0	18.274642254741757	0.0
31	3.2398042348291104E-4	0.0	0.0	18.833791968120327	0.0
32	3.2398042348291104E-4	0.0	0.0	19.365443843055782	0.0
33	3.2398042348291104E-4	0.0	0.0	19.886201876251626	0.0
34	3.2398042348291104E-4	0.0	0.0	20.40392259297732	0.0
35	3.2398042348291104E-4	0.0	0.0	20.926381523396447	0.0
36	3.2398042348291104E-4	0.0	0.0	21.475933316729336	0.0
37	3.2398042348291104E-4	0.0	0.0	22.02034192083943	0.0
38	3.2398042348291104E-4	0.0	0.0	22.54239587572921	0.0
39	3.2398042348291104E-4	0.0	0.0	23.078502481487558	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	35	1.2126839E-7	45.0	31
TACGGGA	865	0.0	42.65896	4
TTACGGG	1425	0.0	42.63158	3
TTATGGG	4840	0.0	41.88533	3
TATGGGA	3040	0.0	41.22533	4
TCGATGG	255	0.0	40.588234	2
CGTTCAT	345	0.0	40.434784	17
TATGGGC	1695	0.0	40.35398	4
CGATAAC	190	0.0	40.263157	16
TATGGGT	920	0.0	39.864132	4
ATGGGCG	880	0.0	39.63068	5
TTTGGGC	5485	0.0	39.46217	4
TTTACGG	790	0.0	39.303795	2
TCGGCGT	665	0.0	38.90977	4
TTTATGG	3035	0.0	38.772655	2
CTATGGG	2515	0.0	38.648113	3
GCGATCG	35	6.2512063E-6	38.571426	9
TACGGGG	1230	0.0	38.23171	4
TCCGATA	195	0.0	38.076927	14
GTACGGG	500	0.0	37.8	3
>>END_MODULE