FastQCFastQC Report
Sat 18 Jun 2016
SRR3550137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404843
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG63130.18541236703131392No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC53180.1561892868481748TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT47830.14047637438789395TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC40100.11777341862752556TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC39230.11521823473211541TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT253.892425E-545.021
ACGGGTA2000.042.755
TTATGGG67600.041.7048843
TATGGGC20500.040.280494
TATGCGT1400.040.17857411
TATGGGA45400.040.0440524
TACGGGA11750.040.0212754
CGTATGG7200.040.02
TTTATGG41200.039.866512
CTACGGG12550.039.442233
TTACGGG22900.039.3995633
TCTACGG7200.039.3752
AACACGT12000.039.187541
GCACGGG8450.039.1420143
TTTACGG14450.039.0830462
TTTCGCG9750.038.7692341
GTACGGG8850.038.644073
AGACACG12500.038.33999624
TTTGGGC71000.038.281694
CACGACC12700.038.0905527
Produced by FastQC (version 0.11.5)