##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3404843
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.471403233570534	34.0	31.0	34.0	31.0	34.0
2	32.61931460569548	34.0	31.0	34.0	31.0	34.0
3	32.67928447802145	34.0	31.0	34.0	31.0	34.0
4	36.055169357294886	37.0	35.0	37.0	35.0	37.0
5	31.90756049544722	37.0	35.0	37.0	0.0	37.0
6	33.583061539107675	37.0	35.0	37.0	17.0	37.0
7	35.327905280801495	37.0	35.0	37.0	32.0	37.0
8	35.859790304574986	37.0	35.0	37.0	35.0	37.0
9	37.9175641872474	39.0	38.0	39.0	35.0	39.0
10	37.6547053711434	39.0	37.0	39.0	35.0	39.0
11	37.55239991976135	39.0	37.0	39.0	35.0	39.0
12	37.28609189909784	39.0	37.0	39.0	35.0	39.0
13	37.18733081084796	39.0	37.0	39.0	34.0	39.0
14	38.22522477541549	40.0	38.0	41.0	33.0	41.0
15	38.381511864130005	40.0	38.0	41.0	33.0	41.0
16	38.52621838951165	40.0	38.0	41.0	34.0	41.0
17	38.53677599818846	40.0	38.0	41.0	34.0	41.0
18	38.529081663971	40.0	38.0	41.0	34.0	41.0
19	38.53275231780144	40.0	38.0	41.0	34.0	41.0
20	38.45584539433977	40.0	38.0	41.0	34.0	41.0
21	38.40930462873031	40.0	37.0	41.0	34.0	41.0
22	38.41874500527631	40.0	37.0	41.0	34.0	41.0
23	38.38248635840184	40.0	37.0	41.0	34.0	41.0
24	38.35513208685393	40.0	37.0	41.0	34.0	41.0
25	38.27079780183697	40.0	37.0	41.0	34.0	41.0
26	38.216599708121635	40.0	37.0	41.0	34.0	41.0
27	38.15848865865475	40.0	37.0	41.0	34.0	41.0
28	38.09697010992871	40.0	37.0	41.0	34.0	41.0
29	38.02244068228696	40.0	36.0	41.0	34.0	41.0
30	37.96870927675667	40.0	36.0	41.0	34.0	41.0
31	37.7515201141433	40.0	36.0	41.0	33.0	41.0
32	37.79545752917242	40.0	36.0	41.0	33.0	41.0
33	37.787845137059186	40.0	36.0	41.0	33.0	41.0
34	37.679686552360856	40.0	36.0	41.0	33.0	41.0
35	37.601204225862986	40.0	36.0	41.0	33.0	41.0
36	37.57881787794621	40.0	35.0	41.0	33.0	41.0
37	37.53052343382647	40.0	35.0	41.0	33.0	41.0
38	37.48471721016211	40.0	35.0	41.0	33.0	41.0
39	37.48780898267556	40.0	35.0	41.0	33.0	41.0
40	37.40426651096688	40.0	35.0	41.0	33.0	41.0
41	37.31269929333012	39.0	35.0	41.0	33.0	41.0
42	37.30617153272559	39.0	35.0	41.0	33.0	41.0
43	37.252814006402055	39.0	35.0	41.0	33.0	41.0
44	37.19372376347456	39.0	35.0	41.0	33.0	41.0
45	37.14802855814497	39.0	35.0	41.0	33.0	41.0
46	37.062802602058305	39.0	35.0	41.0	33.0	41.0
47	36.99192121340103	39.0	35.0	41.0	32.0	41.0
48	36.92138492141928	39.0	35.0	41.0	32.0	41.0
49	36.867061124404266	39.0	35.0	41.0	32.0	41.0
50	36.79530921102676	39.0	35.0	41.0	32.0	41.0
51	36.13006032877287	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	30.0
10	33.0
11	43.0
12	43.0
13	52.0
14	69.0
15	117.0
16	173.0
17	282.0
18	461.0
19	920.0
20	1451.0
21	2268.0
22	3601.0
23	5552.0
24	8196.0
25	11758.0
26	16314.0
27	20492.0
28	25900.0
29	31862.0
30	41307.0
31	55708.0
32	77559.0
33	122176.0
34	216946.0
35	252009.0
36	267583.0
37	426112.0
38	692108.0
39	1122180.0
40	1531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.310372020090206	4.606585384406858	54.206052966318865	16.876989629184077
2	19.529505472058474	4.557655081306245	53.204773318476065	22.708066128159214
3	21.578439886949266	4.698895073869779	50.758258163445426	22.964406875735534
4	18.993709842127814	4.988042033068779	50.13587998036913	25.882368144434263
5	16.373941471016433	16.945774004851323	43.28223063442279	23.398053889709452
6	21.86009751404103	6.623653425429602	52.538633939949655	18.977615120579717
7	85.86081648992332	2.5040508475721204	7.257133441982494	4.377999220522062
8	87.86143737023998	2.8491475231016525	5.275368056618176	4.014047050040192
9	83.61783494863052	4.802923365335788	7.036917708099903	4.542323977933784
10	41.552987905756595	28.445129481741155	14.973201407524517	15.028681204977733
11	32.091876189298596	25.070818243308135	22.70648015194827	20.130825415445
12	29.081898930435262	22.10404415122812	27.835820917440245	20.97823600089637
13	26.885028178979177	22.415365407450505	29.622158789700435	21.077447623869883
14	21.41455567848503	24.421272875136975	31.37087378184545	22.79329766453255
15	19.445624952457425	24.095266653998436	34.236820904811175	22.22228748873296
16	24.858385540831105	23.29728566045483	29.995538707658476	21.84879009105559
17	24.956686695979815	23.47870371702895	28.291142939630404	23.273466647360834
18	25.994943085481477	22.413955650818554	28.65726848491986	22.933832778780108
19	26.113950041161953	24.650005888670933	27.30766146926598	21.928382600901127
20	26.62310714473472	26.99639895290326	26.91430999902198	19.46618390334004
21	26.28350264608383	24.88628697417179	28.75797797431482	20.07223240542956
22	25.042799330248123	22.449669485494635	29.57076141249391	22.936769771763338
23	23.566255477859038	24.97909595244186	28.694685775526214	22.75996279417289
24	22.84651597738868	25.348628409591868	28.58448979879542	23.220365814224035
25	25.188033633268848	25.60029933832485	25.77833985296826	23.433327175438045
26	23.509923952440683	26.194394278972627	25.926922327989864	24.368759440596822
27	23.13046445900736	25.09836723749083	28.747434169505027	23.023734133996783
28	21.203708952218943	26.0046057923963	27.901697670054098	24.889987585330662
29	23.105588128439404	26.142615092678284	27.08970721998048	23.662089558901837
30	25.193232110849163	23.97279404659774	28.41778607706728	22.41618776548581
31	24.524067629550025	25.717279768846907	26.74907477378546	23.009577827817612
32	25.703240942387062	25.839399937089606	26.10681314821271	22.350545972310616
33	26.046957231214478	24.91727225014487	25.86301336067478	23.172757157965872
34	23.29843108771829	24.898211165683705	28.873313688766267	22.93004405783174
35	23.38551292967106	26.140089278712704	27.10377541637015	23.370622375246082
36	25.645529030266594	27.099340556965473	25.084651480259147	22.170478932508782
37	23.631662311595573	27.61792540801441	26.366678287368906	22.383733993021117
38	23.29984084435024	26.792072351059947	27.104744624054618	22.803342180535196
39	23.514358811845362	25.250562213881818	26.10748865659885	25.12759031767397
40	25.294675848489927	23.42956782441951	28.291289789279567	22.984466537810995
41	21.78529230275816	24.275921092396917	27.94839585848746	25.990390746357466
42	22.033321360191938	25.468487093237485	28.506718224599492	23.991473321971085
43	22.414308089976544	25.135549568658526	28.764556838597255	23.685585502767676
44	24.1806156700911	24.173713736580513	27.496891927175497	24.14877866615289
45	23.874639741098193	23.00214723557004	26.285793500610747	26.837419522721017
46	22.952923233171106	24.151451329767628	27.582475902706822	25.313149534354444
47	21.066845079200423	25.903162054755533	29.9530991590508	23.076893706993246
48	21.795483668409968	26.131953808149156	28.701440859387645	23.37112166405323
49	23.902394324789718	24.285818758750406	28.699884253106532	23.111902663353348
50	22.073264464763866	24.84340687661663	27.290362580594756	25.792966078024744
51	21.491387414926326	24.505300244387186	26.457842549568365	27.545469791118123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	566.0
1	1745.0
2	2924.0
3	4789.5
4	6655.0
5	5142.5
6	3630.0
7	3567.0
8	3504.0
9	3632.0
10	3760.0
11	3747.0
12	3734.0
13	3950.5
14	4167.0
15	4303.5
16	4440.0
17	4927.0
18	5414.0
19	6123.0
20	6832.0
21	7505.0
22	8178.0
23	10213.0
24	12248.0
25	15127.0
26	24856.0
27	31706.0
28	35984.0
29	40262.0
30	48896.0
31	57530.0
32	62688.0
33	67846.0
34	79680.5
35	91515.0
36	100698.5
37	109882.0
38	116774.0
39	123666.0
40	135885.0
41	148104.0
42	162262.5
43	176421.0
44	189231.0
45	202041.0
46	225856.0
47	249671.0
48	275062.5
49	300454.0
50	302178.0
51	303902.0
52	284370.0
53	264838.0
54	245925.5
55	227013.0
56	218695.5
57	210378.0
58	199743.5
59	189109.0
60	171455.0
61	153801.0
62	133236.0
63	112671.0
64	101369.0
65	90067.0
66	74853.0
67	59639.0
68	50396.5
69	41154.0
70	34664.0
71	28174.0
72	22186.0
73	16198.0
74	13821.0
75	9309.0
76	7174.0
77	5410.5
78	3647.0
79	2546.0
80	1445.0
81	1099.0
82	753.0
83	471.5
84	190.0
85	127.0
86	64.0
87	42.5
88	21.0
89	12.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3404843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.702879374759352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.23775294821779	14.127179680915821
2	12.455511849294561	5.157299187336622
3	5.161847406462144	3.205953126207005
4	2.773338020118118	2.296643299837573
5	1.642123531728729	1.6998342697916842
6	1.0772651565450773	1.3381494354350387
7	0.7780918775145637	1.1276119597864829
8	0.57838359580928	0.9579364653103275
9	0.4130059311622107	0.7695370776520271
>10	4.246635270627892	20.995538633048685
>50	2.12896379001747	31.903118766783113
>100	0.49723762806567057	13.924595194579487
>500	0.0069208554631393455	0.9840582748359226
>1k	0.002614545397185975	1.1422093604215702
>5k	3.075935761395265E-4	0.37033526805851985
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	6313	0.18541236703131392	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	5318	0.1561892868481748	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	4783	0.14047637438789395	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	4010	0.11777341862752556	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3923	0.11521823473211541	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.810978949690192E-5	0.0	0.0	0.14588044147703727	0.0
2	8.810978949690192E-5	0.0	0.0	0.5645194213066506	0.0
3	8.810978949690192E-5	0.0	0.0	0.8242083408838528	0.0
4	1.1747971932920254E-4	0.0	0.0	1.1462496214950293	0.0
5	1.1747971932920254E-4	0.0	0.0	1.728949029367874	0.0
6	1.1747971932920254E-4	0.0	0.0	2.194080608122019	0.0
7	1.1747971932920254E-4	0.0	0.0	2.5859635818744064	0.0
8	1.1747971932920254E-4	0.0	0.0	3.2237022382529825	0.0
9	1.1747971932920254E-4	0.0	0.0	3.5305592651408597	0.0
10	1.1747971932920254E-4	0.0	0.0	4.142892932214496	0.0
11	1.1747971932920254E-4	0.0	0.0	5.378544620119048	0.0
12	1.7621957899380383E-4	0.0	0.0	6.494043925079659	0.0
13	1.7621957899380383E-4	0.0	0.0	6.835410619520489	0.0
14	1.7621957899380383E-4	0.0	0.0	6.967516563906178	0.0
15	1.7621957899380383E-4	0.0	0.0	7.1452633792512605	0.0
16	1.7621957899380383E-4	0.0	0.0	7.581113137962602	0.0
17	1.7621957899380383E-4	0.0	0.0	8.18378409812141	0.0
18	1.7621957899380383E-4	0.0	0.0	8.806485350425849	0.0
19	1.7621957899380383E-4	0.0	0.0	9.26682963061733	0.0
20	1.7621957899380383E-4	0.0	0.0	9.791875866229368	0.0
21	1.7621957899380383E-4	0.0	0.0	10.559047803378894	0.0
22	1.7621957899380383E-4	0.0	0.0	11.3845777911052	0.0
23	1.7621957899380383E-4	0.0	0.0	12.123965774633367	0.0
24	1.7621957899380383E-4	0.0	0.0	12.692802575625366	0.0
25	1.7621957899380383E-4	0.0	0.0	13.221373202817281	0.0
26	1.7621957899380383E-4	0.0	0.0	13.67096808869014	0.0
27	1.7621957899380383E-4	0.0	0.0	14.160006790327778	0.0
28	2.0558950882610446E-4	0.0	0.0	14.633890608171948	0.0
29	2.0558950882610446E-4	0.0	0.0	15.199026798005077	0.0
30	2.0558950882610446E-4	0.0	0.0	15.859879589161674	0.0
31	2.643293684907057E-4	0.0	0.0	16.39878843165456	0.0
32	2.643293684907057E-4	0.0	0.0	16.924921354670392	0.0
33	2.643293684907057E-4	0.0	0.0	17.434988926067955	0.0
34	2.936992983230064E-4	0.0	0.0	17.969521649015828	0.0
35	2.936992983230064E-4	0.0	0.0	18.59239912089926	0.0
36	2.936992983230064E-4	0.0	0.0	19.144377582167518	0.0
37	3.2306922815530703E-4	0.0	0.0	19.71236265519438	0.0
38	3.2306922815530703E-4	0.0	0.0	20.360204567435268	0.0
39	3.2306922815530703E-4	0.0	0.0	21.506307339281136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAT	25	3.892425E-5	45.0	21
ACGGGTA	200	0.0	42.75	5
TTATGGG	6760	0.0	41.704884	3
TATGGGC	2050	0.0	40.28049	4
TATGCGT	140	0.0	40.178574	11
TATGGGA	4540	0.0	40.044052	4
TACGGGA	1175	0.0	40.021275	4
CGTATGG	720	0.0	40.0	2
TTTATGG	4120	0.0	39.86651	2
CTACGGG	1255	0.0	39.44223	3
TTACGGG	2290	0.0	39.399563	3
TCTACGG	720	0.0	39.375	2
AACACGT	1200	0.0	39.1875	41
GCACGGG	845	0.0	39.142014	3
TTTACGG	1445	0.0	39.083046	2
TTTCGCG	975	0.0	38.769234	1
GTACGGG	885	0.0	38.64407	3
AGACACG	1250	0.0	38.339996	24
TTTGGGC	7100	0.0	38.28169	4
CACGACC	1270	0.0	38.09055	27
>>END_MODULE