Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550136_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1460034 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC | 3965 | 0.27156901825573926 | TruSeq Adapter, Index 13 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT | 3829 | 0.26225416668378954 | TruSeq Adapter, Index 19 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC | 3740 | 0.25615841822861657 | TruSeq Adapter, Index 13 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC | 3616 | 0.2476654653247801 | TruSeq Adapter, Index 13 (95% over 22bp) |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 2533 | 0.17348911052756305 | No Hit |
TCCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG | 1470 | 0.10068258684386802 | TruSeq Adapter, Index 19 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATAC | 20 | 7.0339127E-4 | 45.000004 | 9 |
GATCGAT | 20 | 7.0339127E-4 | 45.000004 | 9 |
GCGAATC | 20 | 7.0339127E-4 | 45.000004 | 24 |
TTCGTAC | 20 | 7.0339127E-4 | 45.000004 | 28 |
TATGCGT | 20 | 7.0339127E-4 | 45.000004 | 43 |
CGCTAGA | 20 | 7.0339127E-4 | 45.000004 | 16 |
TAACACG | 20 | 7.0339127E-4 | 45.000004 | 32 |
ACGCTAT | 20 | 7.0339127E-4 | 45.000004 | 16 |
CGTAAGT | 20 | 7.0339127E-4 | 45.000004 | 44 |
TATGTCG | 30 | 2.165907E-6 | 45.000004 | 1 |
CGGCATA | 25 | 3.8913262E-5 | 45.0 | 13 |
TAACGCG | 25 | 3.8913262E-5 | 45.0 | 44 |
TCGGCAC | 25 | 3.8913262E-5 | 45.0 | 5 |
TTCGATG | 110 | 0.0 | 45.0 | 1 |
TTCGATA | 35 | 1.2122291E-7 | 45.0 | 44 |
CGAACGA | 90 | 0.0 | 42.5 | 22 |
TATGGGC | 805 | 0.0 | 42.484474 | 4 |
TTATGGG | 3020 | 0.0 | 42.46689 | 3 |
TACGGGA | 600 | 0.0 | 42.375 | 4 |
TTACGGG | 990 | 0.0 | 42.272728 | 3 |