FastQCFastQC Report
Sat 18 Jun 2016
SRR3550136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460034
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC39650.27156901825573926TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT38290.26225416668378954TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC37400.25615841822861657TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC36160.2476654653247801TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG25330.17348911052756305No Hit
TCCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG14700.10068258684386802TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC207.0339127E-445.0000049
GATCGAT207.0339127E-445.0000049
GCGAATC207.0339127E-445.00000424
TTCGTAC207.0339127E-445.00000428
TATGCGT207.0339127E-445.00000443
CGCTAGA207.0339127E-445.00000416
TAACACG207.0339127E-445.00000432
ACGCTAT207.0339127E-445.00000416
CGTAAGT207.0339127E-445.00000444
TATGTCG302.165907E-645.0000041
CGGCATA253.8913262E-545.013
TAACGCG253.8913262E-545.044
TCGGCAC253.8913262E-545.05
TTCGATG1100.045.01
TTCGATA351.2122291E-745.044
CGAACGA900.042.522
TATGGGC8050.042.4844744
TTATGGG30200.042.466893
TACGGGA6000.042.3754
TTACGGG9900.042.2727283