##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1460034
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.392850440469196	33.0	31.0	34.0	31.0	34.0
2	32.53695804344282	34.0	31.0	34.0	31.0	34.0
3	32.60214762121978	34.0	31.0	34.0	31.0	34.0
4	36.00518070127134	37.0	35.0	37.0	35.0	37.0
5	31.56022393999044	37.0	35.0	37.0	0.0	37.0
6	33.3847115889082	37.0	35.0	37.0	17.0	37.0
7	35.2290275431942	37.0	35.0	37.0	32.0	37.0
8	35.79731430911883	37.0	35.0	37.0	35.0	37.0
9	37.84842407779545	39.0	38.0	39.0	35.0	39.0
10	37.59371562580049	39.0	37.0	39.0	35.0	39.0
11	37.50896417480689	39.0	37.0	39.0	35.0	39.0
12	37.26180691682522	39.0	37.0	39.0	34.0	39.0
13	37.18282108498843	39.0	37.0	39.0	34.0	39.0
14	38.38867314048851	40.0	38.0	41.0	34.0	41.0
15	38.509220333225116	40.0	38.0	41.0	34.0	41.0
16	38.57818037114204	40.0	38.0	41.0	35.0	41.0
17	38.575751660577765	40.0	38.0	41.0	35.0	41.0
18	38.55425010650437	40.0	38.0	41.0	35.0	41.0
19	38.5713127228544	40.0	38.0	41.0	35.0	41.0
20	38.50596903907717	40.0	38.0	41.0	34.0	41.0
21	38.446172486394154	40.0	37.0	41.0	34.0	41.0
22	38.411557539071005	40.0	37.0	41.0	34.0	41.0
23	38.36126761431583	40.0	37.0	41.0	34.0	41.0
24	38.32754374213203	40.0	37.0	41.0	34.0	41.0
25	38.22905630964758	40.0	37.0	41.0	34.0	41.0
26	38.15246973700612	40.0	37.0	41.0	34.0	41.0
27	38.112559022598106	40.0	37.0	41.0	34.0	41.0
28	38.09147047260543	40.0	37.0	41.0	34.0	41.0
29	38.02676444521155	40.0	36.0	41.0	34.0	41.0
30	37.97938541157261	40.0	36.0	41.0	34.0	41.0
31	37.783144776080555	40.0	36.0	41.0	33.0	41.0
32	37.81765561623907	40.0	36.0	41.0	33.0	41.0
33	37.80907088465063	40.0	36.0	41.0	33.0	41.0
34	37.76309387315638	40.0	36.0	41.0	33.0	41.0
35	37.6687316870703	40.0	36.0	41.0	33.0	41.0
36	37.67683903251568	40.0	36.0	41.0	33.0	41.0
37	37.66602832536776	40.0	36.0	41.0	33.0	41.0
38	37.64138848821329	40.0	35.0	41.0	33.0	41.0
39	37.618240397141435	40.0	35.0	41.0	33.0	41.0
40	37.50929156444302	39.0	35.0	41.0	33.0	41.0
41	37.46040982607254	39.0	35.0	41.0	33.0	41.0
42	37.471986953728475	39.0	35.0	41.0	33.0	41.0
43	37.40576520820748	39.0	35.0	41.0	33.0	41.0
44	37.32427053068627	39.0	35.0	41.0	33.0	41.0
45	37.29956151706056	39.0	35.0	41.0	33.0	41.0
46	37.216252498229494	39.0	35.0	41.0	33.0	41.0
47	37.16978097770326	39.0	35.0	41.0	33.0	41.0
48	37.14025700771352	39.0	35.0	41.0	33.0	41.0
49	37.094173834307966	39.0	35.0	41.0	33.0	41.0
50	37.01015250329787	39.0	35.0	41.0	33.0	41.0
51	36.364465485050346	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	32.0
11	15.0
12	21.0
13	30.0
14	29.0
15	36.0
16	65.0
17	101.0
18	163.0
19	287.0
20	514.0
21	876.0
22	1405.0
23	1943.0
24	3019.0
25	4368.0
26	6110.0
27	7844.0
28	10147.0
29	12785.0
30	16904.0
31	22792.0
32	31412.0
33	49499.0
34	86708.0
35	126136.0
36	117137.0
37	184912.0
38	301808.0
39	472364.0
40	564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.34799463574136	4.823928757823448	54.50948402571447	16.318592580720722
2	19.675911656851827	4.661124329981357	53.768816342633116	21.8941476705337
3	22.132772250509234	4.525648032854029	50.359512175743845	22.982067540892885
4	19.19386808800343	4.861051180999895	50.81765219166129	25.127428539335384
5	16.367016110583727	17.545618800658065	43.233171282312604	22.854193806445604
6	22.53002327343062	6.808334600427113	51.62215400463277	19.0394881215095
7	84.38885669785772	2.48528458926299	8.187480565521078	4.938378147358212
8	86.3660709271154	2.906918606005066	6.094447115615116	4.632563351264422
9	80.93626586778116	5.565897780462647	8.067072410642492	5.4307639411137005
10	39.10511673015834	29.44260202159676	15.46450288143975	15.987778366805156
11	31.672687074410593	25.66138870738627	22.796729391233356	19.869194826969782
12	28.575498926737325	22.59954220244186	28.751590716380576	20.07336815444024
13	25.119004077987224	24.205463708379394	29.48136824210943	21.194163971523952
14	19.850839090048588	27.21320188433968	29.96704186340866	22.968917162203073
15	18.206630804488114	25.22708375284411	34.914666370783145	21.651619071884628
16	20.672737758161798	23.341168767302676	33.70517398909889	22.28091948543664
17	20.919855291041166	23.32801838861287	29.08747330541617	26.66465301492979
18	21.358954654480648	23.737186942221893	30.595451886736885	24.308406516560574
19	24.34073453083969	25.429202333644284	28.24420527193202	21.985857863584
20	27.735518487925624	25.37043657887419	27.41381365091498	19.480231282285207
21	25.848302162826347	25.772824468471285	26.78321189780512	21.595661470897255
22	23.964989856400603	24.556345948108056	27.032247194243418	24.446417001247916
23	22.962478955969516	26.183773802527888	26.824717780544837	24.02902946095776
24	22.802345698798796	24.54319556941825	29.427739354015042	23.226719377767914
25	22.55050224857777	25.88316436466548	26.731432281713985	24.834901105042757
26	21.86688803137461	28.87850556904839	26.2826071173685	22.971999282208497
27	22.024898050319376	28.126331304613455	27.67778010649067	22.1709905385765
28	20.702257618658194	26.54876530272583	29.989438602114742	22.75953847650123
29	21.73689105870137	25.93336867497606	29.181649194470815	23.148091071851752
30	23.027066492972082	26.304661398296204	28.388585471297244	22.27968663743447
31	23.680475934122082	27.848392571679838	25.158181247833955	23.312950246364124
32	24.508264876023436	28.302423094256707	25.931519402972807	21.257792626747047
33	25.248042168880996	26.80464975473174	25.392490859801896	22.55481721658537
34	22.633582505612885	26.548970777392856	26.850196639256346	23.967250077737916
35	21.989967356924563	25.72275714127205	27.849693911237683	24.4375815905657
36	23.219322289754896	27.20635272877207	26.755952258646033	22.818372722827
37	23.75732345959067	26.69150170475482	27.758189192854417	21.7929856428001
38	23.904580304294285	26.877798736193814	26.340619465026155	22.877001494485743
39	24.029303427180462	25.74364706575326	26.700748064771098	23.526301442295182
40	23.095900506426563	25.042225044074318	28.63200446017011	23.229869989329018
41	19.85446914249942	25.5575555089813	28.6268675934944	25.96110775502488
42	23.00076573559246	25.067155970340416	27.82654376541916	24.10553452864796
43	24.104233189090117	24.314159807237363	27.77407923377127	23.80752776990125
44	23.290621999213716	25.57755504323872	26.6434206326702	24.488402324877363
45	22.365849014475007	25.303999769868373	25.763509616899334	26.56664159875729
46	21.97928267423909	26.27151148534897	27.82709169786457	23.922114142547365
47	20.82020007753244	25.968847300816282	29.82985327738943	23.381099344261848
48	22.196606380399363	23.797185544994157	29.975877274090877	24.030330800515603
49	22.90083655586103	23.040627820995947	30.40442893795624	23.654106685186782
50	21.924352446586862	24.46936167239941	27.899213305991506	25.707072575022227
51	20.910608931024893	24.265188344928955	26.720062683471756	28.1041400405744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	752.5
2	1234.0
3	1891.0
4	2548.0
5	1992.0
6	1436.0
7	1506.5
8	1577.0
9	1757.0
10	1937.0
11	2031.0
12	2125.0
13	2013.5
14	1902.0
15	1880.5
16	1859.0
17	1991.0
18	2123.0
19	2607.0
20	3091.0
21	3798.0
22	4505.0
23	5416.5
24	6328.0
25	7069.0
26	10248.0
27	12686.0
28	15287.5
29	17889.0
30	21110.5
31	24332.0
32	27741.5
33	31151.0
34	33941.0
35	36731.0
36	40184.5
37	43638.0
38	48407.5
39	53177.0
40	60132.0
41	67087.0
42	79226.0
43	91365.0
44	98766.0
45	106167.0
46	117999.5
47	129832.0
48	140578.0
49	151324.0
50	145233.0
51	139142.0
52	124464.5
53	109787.0
54	96623.5
55	83460.0
56	76632.0
57	69804.0
58	67172.0
59	64540.0
60	60088.5
61	55637.0
62	50216.0
63	44795.0
64	38517.5
65	32240.0
66	25874.0
67	19508.0
68	16110.0
69	12712.0
70	11395.5
71	10079.0
72	8165.0
73	6251.0
74	5081.0
75	2943.5
76	1976.0
77	1539.5
78	1103.0
79	875.0
80	647.0
81	439.5
82	232.0
83	153.0
84	74.0
85	41.5
86	9.0
87	5.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1460034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.545639634261107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.73823540821418	15.94940080382557
2	12.058418192920065	5.678463386594277
3	5.222065758950041	3.6887063551995567
4	2.9539143308409965	2.7820720937784658
5	1.9030367701147812	2.2404108999935417
6	1.3013490529686624	1.8384657503752253
7	0.9483784875296586	1.563112467298125
8	0.7903446534174493	1.48873363169858
9	0.6096889146290627	1.2919963925573725
>10	4.528079406357892	22.36227024404503
>50	1.5544754908827416	26.52759630446936
>100	0.3844915022929808	12.30867153769308
>500	0.005207559841063612	0.8141657738797847
>1k	0.0023144710404727166	1.4659343585921158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	3965	0.27156901825573926	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	3829	0.26225416668378954	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	3740	0.25615841822861657	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	3616	0.2476654653247801	TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2533	0.17348911052756305	No Hit
TCCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	1470	0.10068258684386802	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3698311135220138E-4	0.0	0.0	0.27054164492059773	0.0
2	1.3698311135220138E-4	0.0	0.0	1.1007962828262903	0.0
3	1.3698311135220138E-4	0.0	0.0	1.5749633227719355	0.0
4	1.3698311135220138E-4	0.0	0.0	2.131114754861873	0.0
5	1.3698311135220138E-4	0.0	0.0	3.065750523617943	0.0
6	1.3698311135220138E-4	0.0	0.0	3.709845113195994	0.0
7	1.3698311135220138E-4	0.0	0.0	4.291338420886089	0.0
8	1.3698311135220138E-4	0.0	0.0	5.208029402055021	0.0
9	1.3698311135220138E-4	0.0	0.0	5.631444199244675	0.0
10	1.3698311135220138E-4	0.0	0.0	6.501971871887915	0.0
11	1.3698311135220138E-4	0.0	0.0	8.161316791252807	0.0
12	1.3698311135220138E-4	0.0	0.0	9.74422513448317	0.0
13	1.3698311135220138E-4	0.0	0.0	10.198050182393013	0.0
14	1.3698311135220138E-4	0.0	0.0	10.355032828002635	0.0
15	1.3698311135220138E-4	0.0	0.0	10.613451467568563	0.0
16	1.3698311135220138E-4	0.0	0.0	11.240697134450294	0.0
17	1.3698311135220138E-4	0.0	0.0	12.124717643561725	0.0
18	1.3698311135220138E-4	0.0	0.0	13.099557955499666	0.0
19	1.3698311135220138E-4	0.0	0.0	13.730502166387906	0.0
20	2.054746670283021E-4	0.0	0.0	14.4363761391858	0.0
21	2.054746670283021E-4	0.0	0.0	15.433476206718474	0.0
22	2.054746670283021E-4	0.0	0.0	16.462013898306477	0.0
23	2.7396622270440277E-4	0.0	0.0	17.41890942265728	0.0
24	2.7396622270440277E-4	0.0	0.0	18.126701159014104	0.0
25	2.7396622270440277E-4	0.0	0.0	18.780658532609515	0.0
26	2.7396622270440277E-4	0.0	0.0	19.33544013358593	0.0
27	2.7396622270440277E-4	0.0	0.0	19.85289383671887	0.0
28	2.7396622270440277E-4	0.0	0.0	20.432948821739767	0.0
29	4.109493340566042E-4	0.0	0.0	21.062660184625837	0.0
30	4.109493340566042E-4	0.0	0.0	21.75517830406689	0.0
31	4.7944088973270486E-4	0.0	0.0	22.417149189676405	0.0
32	4.7944088973270486E-4	0.0	0.0	23.056723336579832	0.0
33	4.7944088973270486E-4	0.0	0.0	23.626847046027695	0.0
34	4.7944088973270486E-4	0.0	0.0	24.244777861337475	0.0
35	4.7944088973270486E-4	0.0	0.0	24.900105066046407	0.0
36	4.7944088973270486E-4	0.0	0.0	25.550911828080714	0.0
37	4.7944088973270486E-4	0.0	0.0	26.212129306577793	0.0
38	4.7944088973270486E-4	0.0	0.0	26.82848481610702	0.0
39	4.7944088973270486E-4	0.0	0.0	27.447306021640593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	20	7.0339127E-4	45.000004	9
GATCGAT	20	7.0339127E-4	45.000004	9
GCGAATC	20	7.0339127E-4	45.000004	24
TTCGTAC	20	7.0339127E-4	45.000004	28
TATGCGT	20	7.0339127E-4	45.000004	43
CGCTAGA	20	7.0339127E-4	45.000004	16
TAACACG	20	7.0339127E-4	45.000004	32
ACGCTAT	20	7.0339127E-4	45.000004	16
CGTAAGT	20	7.0339127E-4	45.000004	44
TATGTCG	30	2.165907E-6	45.000004	1
CGGCATA	25	3.8913262E-5	45.0	13
TAACGCG	25	3.8913262E-5	45.0	44
TCGGCAC	25	3.8913262E-5	45.0	5
TTCGATG	110	0.0	45.0	1
TTCGATA	35	1.2122291E-7	45.0	44
CGAACGA	90	0.0	42.5	22
TATGGGC	805	0.0	42.484474	4
TTATGGG	3020	0.0	42.46689	3
TACGGGA	600	0.0	42.375	4
TTACGGG	990	0.0	42.272728	3
>>END_MODULE