FastQCFastQC Report
Sat 18 Jun 2016
SRR3550132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684627
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC244960.912454504853002No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG195930.7298220572168872No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC188730.7030026890141535No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT125900.46896645232279943No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC109240.4069094142314742No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC108750.4050842072287882No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC91610.3412392112572808No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTAAGT67920.2529960400457866No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT45920.17104797053743406No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC43570.16229442674904185No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT41910.15611107241341163No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTA41170.15335463734813065No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG36620.13640628660890322No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG28600.10653249036085832No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC28250.10522877107322544No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG28000.10429754301063052No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG27030.10068437812776225No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.0349727E-445.022
TCGATCG207.0349727E-445.02
AATTCGG207.0349727E-445.013
CGATCGG253.8922033E-545.03
TATGGGA30050.042.5291184
TACGGGA11050.042.3529434
TCTACGG4850.042.2164962
ACGGGTA2400.042.1875045
ACGGGAG9750.042.05
CGTATGG3450.041.7391322
TAATACG7500.041.74
TTACGGG17150.041.5889243
CCGATGA15300.041.4705918
TCTTACG3150.041.4285741
AATACGG7500.041.45
TTATGGG50300.041.2872773
CCGATCG556.184564E-1140.90909240
TATCCGA501.0822987E-940.530
TTTACGG10750.040.395352
CGATCGA451.9299478E-840.041
Produced by FastQC (version 0.11.5)