##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2684627
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.226375582157225	33.0	31.0	34.0	30.0	34.0
2	32.370961403576736	34.0	31.0	34.0	30.0	34.0
3	32.44661623383807	34.0	31.0	34.0	30.0	34.0
4	35.93489821863521	37.0	35.0	37.0	35.0	37.0
5	31.74326116812503	37.0	35.0	37.0	0.0	37.0
6	33.326950820356046	37.0	35.0	37.0	17.0	37.0
7	35.16086815784837	37.0	35.0	37.0	32.0	37.0
8	35.64811387205746	37.0	35.0	37.0	35.0	37.0
9	37.706702644352454	39.0	37.0	39.0	35.0	39.0
10	37.46578798469955	39.0	37.0	39.0	35.0	39.0
11	37.39543929193888	39.0	37.0	39.0	35.0	39.0
12	37.13917054398991	39.0	37.0	39.0	34.0	39.0
13	37.02735910798781	39.0	37.0	39.0	33.0	39.0
14	38.10240826751724	40.0	37.0	41.0	33.0	41.0
15	38.18830362653732	40.0	37.0	41.0	33.0	41.0
16	38.340950158066654	40.0	37.0	41.0	34.0	41.0
17	38.29795982831134	40.0	37.0	41.0	34.0	41.0
18	38.277879198860774	40.0	37.0	41.0	34.0	41.0
19	38.29901882086413	40.0	37.0	41.0	34.0	41.0
20	38.150782585439245	40.0	37.0	41.0	34.0	41.0
21	38.14159583435613	40.0	37.0	41.0	34.0	41.0
22	38.15091966221006	40.0	37.0	41.0	34.0	41.0
23	38.0517677129821	40.0	37.0	41.0	34.0	41.0
24	38.0404581344075	40.0	37.0	41.0	34.0	41.0
25	37.96629401402876	40.0	36.0	41.0	34.0	41.0
26	37.93212390399113	40.0	36.0	41.0	34.0	41.0
27	37.81958834504756	40.0	36.0	41.0	33.0	41.0
28	37.77151649000029	40.0	36.0	41.0	33.0	41.0
29	37.74399087843488	40.0	36.0	41.0	33.0	41.0
30	37.64325248907949	40.0	36.0	41.0	33.0	41.0
31	37.46950619210788	39.0	36.0	41.0	33.0	41.0
32	37.437389253702655	39.0	35.0	41.0	33.0	41.0
33	37.41315609207536	39.0	35.0	41.0	33.0	41.0
34	37.30065182239469	39.0	35.0	41.0	32.0	41.0
35	37.200668100261225	39.0	35.0	41.0	32.0	41.0
36	37.17681562466592	39.0	35.0	41.0	32.0	41.0
37	37.08109543709424	39.0	35.0	41.0	32.0	41.0
38	37.12614601581523	39.0	35.0	41.0	32.0	41.0
39	37.11260707725878	39.0	35.0	41.0	32.0	41.0
40	36.97234960387421	39.0	35.0	41.0	32.0	41.0
41	36.88073538707612	39.0	35.0	41.0	31.0	41.0
42	36.86800885188147	39.0	35.0	41.0	32.0	41.0
43	36.81374023281447	39.0	35.0	41.0	32.0	41.0
44	36.77972321667033	39.0	35.0	41.0	32.0	41.0
45	36.773766709490744	39.0	35.0	41.0	32.0	41.0
46	36.667773958914964	39.0	35.0	40.0	31.0	41.0
47	36.541757197554816	38.0	35.0	40.0	31.0	41.0
48	36.53804085260261	38.0	35.0	40.0	31.0	41.0
49	36.512866033158424	38.0	35.0	40.0	31.0	41.0
50	36.393489672867034	38.0	35.0	40.0	31.0	41.0
51	35.726113907071635	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	14.0
10	17.0
11	31.0
12	26.0
13	25.0
14	30.0
15	60.0
16	111.0
17	197.0
18	372.0
19	737.0
20	1604.0
21	2996.0
22	4851.0
23	6688.0
24	9145.0
25	11774.0
26	14890.0
27	18400.0
28	22448.0
29	29063.0
30	38455.0
31	52121.0
32	70303.0
33	105272.0
34	176328.0
35	222388.0
36	233505.0
37	364163.0
38	564653.0
39	733230.0
40	730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.46574216827887	4.4945163704306035	51.07018591409533	15.969555547195197
2	19.135693710895403	9.29495978398489	50.261581962782905	21.307764542336795
3	20.513911243535883	9.282742071803643	47.71538094491339	22.487965739747086
4	18.511621912466797	4.567599148783053	52.37468743330079	24.54609150544936
5	19.137742412633113	16.532389788227565	41.4039268769926	22.925940922146726
6	21.041694060292173	9.845054825120956	49.81742342604764	19.295827688539227
7	79.16716176958661	2.5454932845419496	12.980350715388022	5.306994230483415
8	79.2454221759671	6.418321800384187	8.388986626447547	5.947269397201175
9	74.28339206899133	5.156098035220536	10.204881348507634	10.355628547280498
10	39.73639540986513	26.498504261485863	17.556703407959468	16.20839692068954
11	32.351123638404886	23.018318745956144	24.620962241681994	20.00959537395698
12	27.297423440947288	20.232978361612247	29.60638479759013	22.86321339985033
13	25.036923192681886	21.944314796804175	32.29744765287691	20.72131435763702
14	20.327814627506914	26.08366078416108	30.42579099442865	23.16273359390336
15	17.10915520107635	23.388053535928826	36.421856742109796	23.080934520885023
16	20.28412140680996	23.75804906975904	30.644741336505966	25.313088186925036
17	20.50742989622022	23.923509671920904	29.890372107559077	25.6786883242998
18	21.79531830678899	22.642884840240377	30.483489885187034	25.078306967783607
19	23.416102125174188	24.341444826413504	28.249175770041795	23.993277278370513
20	25.472253687383763	25.77721225332234	29.256354793421952	19.494179265871946
21	23.584095667666308	27.967013667075534	28.08662059943523	20.362270065822923
22	21.635370574757683	22.714626650182687	29.986027854148826	25.6639749209108
23	21.77010065085392	26.267298958104796	27.92406542882866	24.038534962212626
24	22.91823035378844	23.29660694018201	28.370421663791657	25.4147410422379
25	21.927552691677466	27.668424701085105	25.132094700679087	25.27192790655834
26	21.48570360053743	24.738781216161502	27.67073414668034	26.10478103662073
27	22.325745811243053	24.90919595161637	28.661151064933787	24.10390717220679
28	18.78674393127984	24.736360023198753	30.63893047339537	25.837965572126038
29	23.40109072880516	22.45295156459352	28.990172564009825	25.1557851425915
30	22.552034230453614	23.952526738351363	31.057834105073066	22.437604926121953
31	23.975621194303713	25.23188509986676	25.065940259112345	25.726553446717176
32	25.878865108635203	26.857325058564935	25.04359078561007	22.220219047189797
33	23.68496629140659	23.78192575728397	26.891594251268426	25.641513700041013
34	23.39770105865731	25.186217675677103	27.21558711880645	24.200494146859135
35	22.993622577736126	24.84043407147436	27.390434499839273	24.775508850950242
36	24.967490828334814	25.509689055500072	26.58108556607678	22.941734550088334
37	22.062469013386217	26.207104376138656	28.92949374345114	22.800932867023985
38	22.560638777751993	25.414405800135363	26.124485822425235	25.900469599687405
39	25.479070276801952	23.96705389612784	26.939645619298325	23.61423020777188
40	22.904820669687073	24.182949810159847	30.55206551971652	22.360164000436562
41	21.126733806968343	27.266879160494177	26.19585514114251	25.410531891394967
42	23.54606431358993	24.542403842321484	27.60521294019616	24.30631890389242
43	25.67731010676716	21.646061072916275	27.51842248476232	25.15820633555425
44	23.05035299130941	21.253753314706287	29.008797125261722	26.687096568722584
45	22.65510255242162	20.946634299662488	27.27056682362205	29.12769632429384
46	24.6092660172158	23.296532441937	28.01186161057011	24.08233993027709
47	19.004688547049554	24.42197742926671	33.070664937810726	23.50266908587301
48	21.03457947789395	24.440937232621142	29.21932916565318	25.30515412383173
49	23.36436309401641	21.040613835739563	31.849079965298717	23.745943104945304
50	22.659758692734595	21.68967979536822	29.117154822625267	26.533406689271917
51	20.56270759401585	21.840724987121117	26.977118236537144	30.619449182325887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	629.0
1	1536.5
2	2444.0
3	3951.5
4	5459.0
5	4134.0
6	2809.0
7	2764.5
8	2720.0
9	3104.0
10	3488.0
11	3686.5
12	3885.0
13	4289.0
14	4693.0
15	4343.0
16	3993.0
17	4169.0
18	4345.0
19	5135.5
20	5926.0
21	6201.0
22	6476.0
23	7233.0
24	7990.0
25	10336.5
26	15855.0
27	19027.0
28	22639.5
29	26252.0
30	30978.5
31	35705.0
32	42046.0
33	48387.0
34	54027.0
35	59667.0
36	63074.5
37	66482.0
38	71492.5
39	76503.0
40	89741.5
41	102980.0
42	118778.0
43	134576.0
44	155944.0
45	177312.0
46	243304.5
47	309297.0
48	309252.0
49	309207.0
50	299480.5
51	289754.0
52	249572.0
53	209390.0
54	187959.0
55	166528.0
56	150887.5
57	135247.0
58	127762.0
59	120277.0
60	110414.5
61	100552.0
62	86591.5
63	72631.0
64	61706.5
65	50782.0
66	42748.0
67	34714.0
68	29643.0
69	24572.0
70	20991.0
71	17410.0
72	15136.0
73	12862.0
74	10353.5
75	6111.0
76	4377.0
77	3344.0
78	2311.0
79	1752.0
80	1193.0
81	1040.5
82	888.0
83	506.5
84	125.0
85	85.5
86	46.0
87	88.0
88	130.0
89	92.5
90	55.0
91	29.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2684627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.71729754055011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37971501717146	14.625331416209061
2	13.57531703230847	6.1678903246049845
3	6.236561612947437	4.2503347737390005
4	3.5163246673539685	3.19525574869824
5	2.1914622113611197	2.4892049552181237
6	1.4724962926425136	2.007068184439016
7	1.012479044800884	1.6100551400019554
8	0.7512265660655131	1.365266993734079
9	0.5764120916313963	1.1785072492405138
>10	4.597001078652138	23.91536261111412
>50	1.4625869150021387	22.76083157357984
>100	0.21171341425445972	7.6222561090629135
>500	0.00896744048676084	1.3905015987117908
>1k	0.00632995799065471	2.8962824945963783
>5k	3.5166433281415055E-4	0.6372316797635574
>10k+	0.0010549929984424516	3.8886191472865343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	24496	0.912454504853002	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	19593	0.7298220572168872	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	18873	0.7030026890141535	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	12590	0.46896645232279943	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	10924	0.4069094142314742	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	10875	0.4050842072287882	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	9161	0.3412392112572808	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTAAGT	6792	0.2529960400457866	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	4592	0.17104797053743406	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC	4357	0.16229442674904185	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	4191	0.15611107241341163	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTA	4117	0.15335463734813065	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	3662	0.13640628660890322	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2860	0.10653249036085832	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	2825	0.10522877107322544	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	2800	0.10429754301063052	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	2703	0.10068437812776225	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.724912250379662E-5	0.0	0.0	0.4854305644694775	0.0
2	3.724912250379662E-5	0.0	0.0	1.7151358456873151	0.0
3	3.724912250379662E-5	0.0	0.0	2.392101398071315	0.0
4	3.724912250379662E-5	0.0	0.0	3.315171902837899	0.0
5	3.724912250379662E-5	0.0	0.0	5.324911058407742	0.0
6	3.724912250379662E-5	0.0	0.0	6.231517451027647	0.0
7	3.724912250379662E-5	0.0	0.0	7.190347113397876	0.0
8	3.724912250379662E-5	0.0	0.0	8.56875089165087	0.0
9	3.724912250379662E-5	0.0	0.0	9.123092332752371	0.0
10	3.724912250379662E-5	0.0	0.0	11.086940569397536	0.0
11	3.724912250379662E-5	0.0	0.0	13.235581702784037	0.0
12	3.724912250379662E-5	0.0	0.0	16.22731947492147	0.0
13	3.724912250379662E-5	0.0	0.0	16.902124578200247	0.0
14	3.724912250379662E-5	0.0	0.0	17.173447186517905	0.0
15	3.724912250379662E-5	0.0	0.0	17.71881158909599	0.0
16	3.724912250379662E-5	0.0	0.0	18.555575877021276	0.0
17	3.724912250379662E-5	0.0	0.0	19.641760289231986	0.0
18	3.724912250379662E-5	0.0	0.0	20.806652097293217	0.0
19	3.724912250379662E-5	0.0	0.0	21.9347417723207	0.0
20	7.449824500759324E-5	0.0	0.0	22.850101708728996	0.0
21	7.449824500759324E-5	0.0	0.0	24.074890105776333	0.0
22	4.097403475417628E-4	0.0	0.0	25.345532172625845	0.0
23	4.097403475417628E-4	0.0	0.0	26.468853959972837	0.0
24	4.097403475417628E-4	0.0	0.0	27.341787145849313	0.0
25	4.097403475417628E-4	0.0	0.0	28.15061459189675	0.0
26	4.097403475417628E-4	0.0	0.0	28.860024129981557	0.0
27	4.097403475417628E-4	0.0	0.0	29.5913361520986	0.0
28	4.469894700455594E-4	0.0	0.0	30.281674139461458	0.0
29	4.469894700455594E-4	0.0	0.0	31.051315508634904	0.0
30	4.469894700455594E-4	0.0	0.0	31.92760111553672	0.0
31	4.469894700455594E-4	0.0	0.0	32.65850339730622	0.0
32	4.469894700455594E-4	0.0	0.0	33.40937120873775	0.0
33	4.469894700455594E-4	0.0	0.0	34.12537384150573	0.0
34	4.469894700455594E-4	0.0	0.0	34.85031626367462	0.0
35	4.84238592549356E-4	0.0	0.0	35.62982865031157	0.0
36	4.84238592549356E-4	0.0	0.0	36.34367083397433	0.0
37	4.84238592549356E-4	0.0	0.0	37.072710659618636	0.0
38	4.84238592549356E-4	0.0	0.0	37.81046677992883	0.0
39	4.84238592549356E-4	0.0	0.0	38.801293438529825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	7.0349727E-4	45.0	22
TCGATCG	20	7.0349727E-4	45.0	2
AATTCGG	20	7.0349727E-4	45.0	13
CGATCGG	25	3.8922033E-5	45.0	3
TATGGGA	3005	0.0	42.529118	4
TACGGGA	1105	0.0	42.352943	4
TCTACGG	485	0.0	42.216496	2
ACGGGTA	240	0.0	42.187504	5
ACGGGAG	975	0.0	42.0	5
CGTATGG	345	0.0	41.739132	2
TAATACG	750	0.0	41.7	4
TTACGGG	1715	0.0	41.588924	3
CCGATGA	1530	0.0	41.47059	18
TCTTACG	315	0.0	41.428574	1
AATACGG	750	0.0	41.4	5
TTATGGG	5030	0.0	41.287277	3
CCGATCG	55	6.184564E-11	40.909092	40
TATCCGA	50	1.0822987E-9	40.5	30
TTTACGG	1075	0.0	40.39535	2
CGATCGA	45	1.9299478E-8	40.0	41
>>END_MODULE