FastQCFastQC Report
Sat 18 Jun 2016
SRR3550130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324481
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91112.8078685654938194No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG45761.4102520640653844No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC42271.302695689424034No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC40831.258317127967431RNA PCR Primer, Index 17 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC27140.8364126096751427Illumina PCR Primer Index 9 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21750.6703011886674413No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC18820.5800031434814364No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAGATAT15360.47337132220376543No Hit
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC14910.459503021748577RNA PCR Primer, Index 17 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA12700.3913942572908737No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11760.3624249185622579No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT10930.3368456088337992RNA PCR Primer, Index 17 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCT10680.32914099746980563No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.31927909492389384No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.31927909492389384No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9840.3032535032867872No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.24839667037515295No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAGATATCGTA7450.2295974186470086No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCT7310.22528283628317222Illumina PCR Primer Index 9 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTC6970.21480456482814095No Hit
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC5870.18090427482656918Illumina PCR Primer Index 9 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.18090427482656918No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG5380.16580323655314178No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5200.1602559163710664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.1466958003704377No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC4220.1300538398242116No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4060.1251228885512557No Hit
ACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC3900.12019193727829981Illumina PCR Primer Index 9 (95% over 23bp)
GATAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATAT3400.10478271455031266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG551.8189894E-1245.0000044
ACCGACT551.8189894E-1245.00000417
CAGGTTG253.8845974E-545.05
CAAACGA207.025794E-445.015
CGAAATA253.8845974E-545.044
CACGGGC207.025794E-445.04
CTATCGA253.8845974E-545.041
GCGAAAT253.8845974E-545.043
GGGTATC253.8845974E-545.07
CGACCCT351.2083183E-745.012
TAACGGG453.8380676E-1045.03
TAACGCC207.025794E-445.012
CGACAAA207.025794E-445.012
TGACGGA207.025794E-445.06
ACTAGCG207.025794E-445.01
TGCGAAG253.8845974E-545.01
CGAGGGT207.025794E-445.04
CCATAGG207.025794E-445.01
GCGGGCG253.8845974E-545.05
CGAATTT253.8845974E-545.013
Produced by FastQC (version 0.11.5)