SRR3550128_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652849
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18557	2.842464337082541	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	9745	1.4926882020191499	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	9634	1.475685801770394	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	8122	1.2440855389224768	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	4449	0.6814745829433759	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	2850	0.4365481144950823	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGATCTGT	2670	0.408976654632235	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCT	2320	0.3553654826766986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2261	0.3463281708327653	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2093	0.3205948082941078	No Hit
GAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	2052	0.3143146424364593	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2004	0.3069622531397	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTC	1686	0.25825267404866975	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTA	1551	0.23757407915153428	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1445	0.22133755278785752	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1433	0.2194994554636677	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	1431	0.2191931059096361	TruSeq Adapter, Index 13 (95% over 22bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1323	0.2026502299919277	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.17063670159562164	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	982	0.15041763102953365	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	918	0.14061444530052125	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.1236120450517654	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTT	738	0.11304298543767395	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.10921361601227848	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	702	0.10752869346510449	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	667	0.10216757626955084	No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	663	0.10155487716148758	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	50	2.1827873E-11	45.0	5
TCGTTTA	20	7.0310454E-4	45.0	38
ACGCATG	20	7.0310454E-4	45.0	1
GCGAACC	25	3.888947E-5	45.0	33
CGTTGTT	20	7.0310454E-4	45.0	1
TAACGTC	20	7.0310454E-4	45.0	32
CCGTCGA	80	0.0	45.0	41
ACGATAG	25	3.888947E-5	45.0	1
TGCGATG	20	7.0310454E-4	45.0	1
ACGTCGA	25	3.888947E-5	45.0	8
CTCGAGG	25	3.888947E-5	45.0	2
CGTCGAC	25	3.888947E-5	45.0	9
CGTCGAA	80	0.0	45.0	42
ATACGTC	40	6.8066583E-9	45.0	6
ATTGCGG	20	7.0310454E-4	45.0	2
GCGTATG	20	7.0310454E-4	45.0	1
CGCCCAC	35	1.2108467E-7	45.0	34
GCATCGT	20	7.0310454E-4	45.0	35
TACGTCG	25	3.888947E-5	45.0	7
GTACGAG	20	7.0310454E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content