##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652849
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69362134276073	31.0	31.0	34.0	30.0	34.0
2	31.780610830375785	31.0	31.0	34.0	30.0	34.0
3	31.788767387251877	33.0	31.0	34.0	30.0	34.0
4	35.604752400631696	37.0	35.0	37.0	33.0	37.0
5	33.04483425723253	37.0	35.0	37.0	28.0	37.0
6	34.06153643491834	37.0	35.0	37.0	28.0	37.0
7	35.31736282049907	37.0	35.0	37.0	32.0	37.0
8	35.10197763954605	37.0	35.0	37.0	32.0	37.0
9	37.15836433846111	39.0	37.0	39.0	34.0	39.0
10	36.79483464016947	39.0	37.0	39.0	32.0	39.0
11	36.68969700497359	39.0	37.0	39.0	32.0	39.0
12	37.00294555096201	39.0	37.0	39.0	33.0	39.0
13	36.92887329229271	39.0	37.0	39.0	33.0	39.0
14	37.94475292142594	40.0	37.0	41.0	33.0	41.0
15	38.006549753465194	40.0	37.0	41.0	33.0	41.0
16	38.07686463485431	40.0	37.0	41.0	33.0	41.0
17	37.875251398102776	40.0	37.0	41.0	33.0	41.0
18	37.77423722790416	39.0	37.0	40.0	33.0	41.0
19	37.56741604873409	39.0	37.0	40.0	33.0	41.0
20	37.12070478778401	39.0	35.0	40.0	32.0	41.0
21	37.21955613013117	39.0	35.0	40.0	32.0	41.0
22	37.21784210437636	39.0	35.0	40.0	33.0	41.0
23	36.84474204601677	39.0	35.0	40.0	31.0	41.0
24	36.629217476016656	38.0	35.0	40.0	31.0	41.0
25	36.47898671821508	38.0	35.0	40.0	31.0	41.0
26	36.671170515693525	38.0	35.0	40.0	31.0	41.0
27	36.52434176968947	38.0	35.0	40.0	31.0	41.0
28	36.58592568878868	39.0	35.0	40.0	31.0	41.0
29	36.561719478776865	39.0	35.0	40.0	31.0	41.0
30	36.36439207228624	38.0	35.0	40.0	31.0	41.0
31	36.276295131033365	39.0	35.0	40.0	30.0	41.0
32	35.77689634203315	38.0	35.0	40.0	27.0	41.0
33	35.6668035028008	39.0	35.0	40.0	26.0	41.0
34	35.378303405534815	39.0	35.0	40.0	24.0	41.0
35	35.12922436888162	39.0	35.0	40.0	22.0	41.0
36	35.01861533065073	39.0	35.0	40.0	21.0	41.0
37	34.942648299989735	39.0	35.0	40.0	21.0	41.0
38	34.80285793498956	38.0	35.0	40.0	20.0	41.0
39	34.71916630032366	38.0	35.0	40.0	20.0	41.0
40	34.67485896432406	38.0	35.0	40.0	20.0	41.0
41	34.583746011711746	38.0	34.0	40.0	20.0	41.0
42	34.56708365946796	38.0	35.0	40.0	18.0	41.0
43	34.41910150739298	38.0	34.0	40.0	18.0	41.0
44	34.41044100550051	38.0	34.0	40.0	18.0	41.0
45	34.44728107112058	38.0	34.0	40.0	18.0	41.0
46	34.30327839975247	38.0	34.0	40.0	18.0	41.0
47	34.15892495814499	38.0	34.0	40.0	18.0	41.0
48	34.11681874369111	38.0	34.0	40.0	18.0	41.0
49	34.140046166877795	38.0	34.0	40.0	18.0	41.0
50	34.01593170855742	38.0	34.0	40.0	17.0	41.0
51	33.11699029944137	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	5.0
10	13.0
11	23.0
12	23.0
13	25.0
14	43.0
15	38.0
16	108.0
17	159.0
18	364.0
19	775.0
20	1539.0
21	2915.0
22	4593.0
23	6429.0
24	7848.0
25	10046.0
26	12290.0
27	13511.0
28	13211.0
29	12586.0
30	13375.0
31	15847.0
32	19234.0
33	25210.0
34	36868.0
35	51507.0
36	65914.0
37	85387.0
38	130488.0
39	122452.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0887525292985	19.138269339464408	20.416972377992458	18.356005753244624
2	35.23310903440152	31.085442422367194	20.45955496600286	13.221893577228425
3	24.15259883985424	30.505829066139338	33.17214240965369	12.169429684352737
4	22.40901035308318	22.64229553847827	41.48708200518037	13.46161210325818
5	24.275445011020924	30.453902816730977	30.430160726293522	14.840491445954578
6	19.596568272295738	36.84588626160107	32.21587227674394	11.341673189359254
7	58.84791123215323	7.979640008639058	28.527423646203026	4.645025113004691
8	56.277025774719725	14.531691095490688	22.952780811489333	6.23850231830025
9	50.674505130589154	10.724225663208491	24.18844173767594	14.412827468526412
10	28.657469031889455	27.115152202117184	30.916184293764715	13.311194472228646
11	24.077390024339472	22.920614108316013	35.511887128570315	17.490108738774204
12	20.264410300084705	19.787424044457445	39.553403620132684	20.39476203532517
13	21.334489292317212	21.675149996400393	42.51748873016578	14.472871981116613
14	15.429142114026368	29.09049412651318	37.25026767292284	18.23009608653762
15	13.095677560967392	24.889369517300324	43.532424802672594	18.48252811905969
16	13.959583303336606	25.654171178940306	37.489067150290495	22.89717836743259
17	13.884987186929903	27.298961934536166	38.53953977106498	20.276511107468956
18	13.273973001413802	25.677606919823724	39.966056469413296	21.082363609349176
19	15.408463519129231	25.81607691824603	37.39180116688545	21.383658395739293
20	17.518446072522128	27.02278781157664	40.58319764601003	14.875568469891201
21	15.809934609687692	29.629362992054826	38.29461330261669	16.266089095640798
22	13.376446927237387	25.08022528946203	39.04593558387928	22.497392199421306
23	16.16392151937125	26.72011445219339	38.13653693273636	18.979427095699005
24	16.73158724299187	25.529946434780477	36.42358340136846	21.314882920859187
25	13.724919544948373	31.975387876829096	34.03283148170557	20.26686109651696
26	13.222506276336487	27.512181224142186	39.04486336044016	20.220449139081165
27	18.499224169754413	26.740486697536493	34.883411018474405	19.876878114234685
28	13.20335942920951	28.48484105819263	37.48048936277761	20.83131014982025
29	16.30346374123266	27.64529010536893	35.71178021257595	20.339465940822457
30	15.322226119669327	30.01704835268186	34.169922907134726	20.490802620514085
31	18.212480987180804	27.904002303748648	30.358628105427137	23.524888603643415
32	18.126243587720896	32.09608960111756	31.137062322221524	18.640604488940017
33	18.432899491306564	25.793866575578733	32.607693356350396	23.165540576764304
34	16.953384320110775	27.725094164194168	31.67164229400673	23.649879221688323
35	16.671849079955702	26.561119033650964	32.59942191839154	24.167609968001788
36	20.337781018275283	29.54052162138565	29.592447870794015	20.529249489545055
37	18.07753400862987	28.035119912874183	32.34591766243036	21.541428416065582
38	19.403874402809837	27.280887310848296	31.319033957316318	21.99620432902555
39	20.39430251099412	26.89473369799142	31.330062541261455	21.380901249753006
40	17.041766166448905	24.117828165471646	36.92186095100092	21.91854471707853
41	18.861788866950857	26.231333738735913	30.549943401919894	24.35693399239334
42	19.415362511086023	25.239680232335505	34.908838031459034	20.436119225119437
43	23.172892966061063	25.568546478588466	30.132235784997757	21.126324770352717
44	19.83643997310251	24.650263690378633	31.267567232239003	24.245729104279857
45	19.397134712621142	22.973459406386468	31.322250627633647	26.307155253358737
46	21.58217290675179	27.093707733334966	31.229733062316097	20.094386297597147
47	16.615021237682832	24.743853479135296	38.60908111983016	20.03204416335171
48	18.854283302877082	24.733131244744193	32.929666737637646	23.48291871474108
49	18.931942914824102	21.961127305088926	37.83248500035996	21.274444779727013
50	19.099822470433438	21.394533804907415	34.81463554359431	24.69100818106484
51	18.080137979839137	21.068884228971786	31.894817944118774	28.9561598470703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3589.0
1	6074.0
2	8559.0
3	19468.0
4	30377.0
5	19516.0
6	8655.0
7	8330.5
8	8006.0
9	8160.0
10	8314.0
11	8114.0
12	7914.0
13	7812.5
14	7711.0
15	7654.0
16	7597.0
17	6965.0
18	6333.0
19	6425.0
20	6517.0
21	6800.0
22	7083.0
23	6828.5
24	6574.0
25	7888.5
26	9988.0
27	10773.0
28	11128.0
29	11483.0
30	12065.5
31	12648.0
32	13161.5
33	13675.0
34	13640.0
35	13605.0
36	14438.0
37	15271.0
38	16328.0
39	17385.0
40	19577.0
41	21769.0
42	25319.0
43	28869.0
44	33346.5
45	37824.0
46	60968.5
47	84113.0
48	75010.0
49	65907.0
50	62960.0
51	60013.0
52	47455.5
53	34898.0
54	28692.5
55	22487.0
56	20205.5
57	17924.0
58	16401.5
59	14879.0
60	14468.0
61	14057.0
62	12269.0
63	10481.0
64	8677.5
65	6874.0
66	5513.0
67	4152.0
68	3341.0
69	2530.0
70	2212.5
71	1895.0
72	1576.0
73	1257.0
74	988.0
75	580.0
76	441.0
77	367.0
78	293.0
79	202.5
80	112.0
81	80.5
82	49.0
83	34.0
84	19.0
85	12.5
86	6.0
87	4.5
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	652849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.24518716516176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50343431597041	28.11152602696462
2	10.203722303390297	7.804865385489945
3	4.329059879315988	4.966971160008976
4	2.580340108478497	3.9474236159413567
5	1.6525380859974557	3.1600814198265437
6	1.320227946367905	3.02954189457106
7	1.0310735948615273	2.7603521831574693
8	0.8498227250426224	2.600130334117031
9	0.7397731456190828	2.546348617256606
>10	3.6306224727540943	21.424099261506086
>50	0.09604524148556046	2.4777144233475874
>100	0.050920537438790096	3.7758664133192945
>500	0.0045538692018430174	1.2447946050619043
>1k	0.006209821638876841	4.857897236391421
>5k	0.0012419643277753682	4.354247829281026
>10k+	4.139881092584561E-4	2.9381395937590638
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18557	2.842464337082541	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	9745	1.4926882020191499	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	9634	1.475685801770394	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	8122	1.2440855389224768	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	4449	0.6814745829433759	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	2850	0.4365481144950823	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGATCTGT	2670	0.408976654632235	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCT	2320	0.3553654826766986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2261	0.3463281708327653	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2093	0.3205948082941078	No Hit
GAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	2052	0.3143146424364593	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2004	0.3069622531397	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTC	1686	0.25825267404866975	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTA	1551	0.23757407915153428	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1445	0.22133755278785752	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1433	0.2194994554636677	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	1431	0.2191931059096361	TruSeq Adapter, Index 13 (95% over 22bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1323	0.2026502299919277	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.17063670159562164	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	982	0.15041763102953365	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	918	0.14061444530052125	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.1236120450517654	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTT	738	0.11304298543767395	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.10921361601227848	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	702	0.10752869346510449	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	667	0.10216757626955084	No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	663	0.10155487716148758	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23742090437451846	0.0
2	0.0	0.0	0.0	1.5539581128254774	0.0
3	0.0	0.0	0.0	2.0395221559656216	0.0
4	0.0	0.0	0.0	2.984151005822173	0.0
5	0.0	0.0	0.0	6.185197495898746	0.0
6	0.0	0.0	0.0	7.363571055481436	0.0
7	0.0	0.0	0.0	8.774157577020107	0.0
8	0.0	0.0	0.0	10.465360290051757	0.0
9	0.0	0.0	0.0	11.061669696974338	0.0
10	0.0	0.0	0.0	14.285079704495221	0.0
11	0.0	0.0	0.0	16.888131865102036	0.0
12	0.0	0.0	0.0	20.1355903126144	0.0
13	0.0	0.0	0.0	20.997198433328382	0.0
14	0.0	0.0	0.0	21.421645740439214	0.0
15	0.0	0.0	0.0	22.38588096175379	0.0
16	0.0	0.0	0.0	23.83353577933029	0.0
17	0.0	0.0	0.0	25.477407486264052	0.0
18	0.0	0.0	0.0	27.08926566480151	0.0
19	0.0	0.0	0.0	28.667272217618468	0.0
20	0.0	0.0	0.0	29.764156795828743	0.0
21	0.0	0.0	0.0	30.9518740168094	0.0
22	0.0	0.0	0.0	32.25554454399103	0.0
23	0.0	0.0	0.0	33.34844657799889	0.0
24	0.0	0.0	0.0	34.118456182057415	0.0
25	0.0	0.0	0.0	34.7496894381396	0.0
26	0.0	0.0	0.0	35.331140891691646	0.0
27	0.0	0.0	0.0	35.85469227953171	0.0
28	0.0	0.0	0.0	36.33152536038196	0.0
29	0.0	0.0	0.0	36.79916795461125	0.0
30	0.0	0.0	0.0	37.25072719725388	0.0
31	0.0	0.0	0.0	37.66598401774377	0.0
32	0.0	0.0	0.0	38.07863686702438	0.0
33	0.0	0.0	0.0	38.47367461694817	0.0
34	3.0634955403163673E-4	0.0	0.0	38.85140361706918	0.0
35	4.595243310474551E-4	0.0	0.0	39.20906672140112	0.0
36	4.595243310474551E-4	0.0	0.0	39.55125917325446	0.0
37	4.595243310474551E-4	0.0	0.0	39.88793733313523	0.0
38	4.595243310474551E-4	0.0	0.0	40.20868531620635	0.0
39	4.595243310474551E-4	0.0	0.0	40.52591027940611	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	50	2.1827873E-11	45.0	5
TCGTTTA	20	7.0310454E-4	45.0	38
ACGCATG	20	7.0310454E-4	45.0	1
GCGAACC	25	3.888947E-5	45.0	33
CGTTGTT	20	7.0310454E-4	45.0	1
TAACGTC	20	7.0310454E-4	45.0	32
CCGTCGA	80	0.0	45.0	41
ACGATAG	25	3.888947E-5	45.0	1
TGCGATG	20	7.0310454E-4	45.0	1
ACGTCGA	25	3.888947E-5	45.0	8
CTCGAGG	25	3.888947E-5	45.0	2
CGTCGAC	25	3.888947E-5	45.0	9
CGTCGAA	80	0.0	45.0	42
ATACGTC	40	6.8066583E-9	45.0	6
ATTGCGG	20	7.0310454E-4	45.0	2
GCGTATG	20	7.0310454E-4	45.0	1
CGCCCAC	35	1.2108467E-7	45.0	34
GCATCGT	20	7.0310454E-4	45.0	35
TACGTCG	25	3.888947E-5	45.0	7
GTACGAG	20	7.0310454E-4	45.0	1
>>END_MODULE