SRR3550127_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410979
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10615	2.5828570316244868	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	4741	1.153586922932802	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	4504	1.0959197428579075	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	4394	1.0691543850172394	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	3119	0.7589195555004027	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	2503	0.6090335515926605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2180	0.5304407281150618	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1708	0.4155930108351035	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	1444	0.3513561520174997	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	1287	0.31315468673581864	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	1227	0.29855540064090863	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.22555897016635884	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	927	0.22555897016635884	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	861	0.2094997554619579	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	836	0.20341671958907875	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	809	0.19684704084636928	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	798	0.19417050506230246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	766	0.18638421914501713	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA	715	0.17397482596434366	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	689	0.16764846865654937	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.16351200426299153	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.1622953970884157	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	650	0.1581589326948579	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	645	0.15694232552028206	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	543	0.13212353915893513	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448	0.10900800284199437	No Hit
ACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	416	0.10122171692470905	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCG	30	2.1620854E-6	45.000004	31
TCGTTAG	20	7.0279907E-4	45.000004	1
TTGTGCG	20	7.0279907E-4	45.000004	1
CATGCGG	30	2.1620854E-6	45.000004	2
GCGAAAG	40	6.7975634E-9	45.000004	1
CGGCAGT	20	7.0279907E-4	45.000004	35
GACCGAT	45	3.8380676E-10	45.000004	9
GCCGGAT	30	2.1620854E-6	45.000004	1
TGAGTCG	20	7.0279907E-4	45.000004	1
CGTTCAC	20	7.0279907E-4	45.000004	18
CGTTAGG	35	1.2093733E-7	45.000004	2
CGGGGTA	20	7.0279907E-4	45.000004	6
GGGACGT	20	7.0279907E-4	45.000004	8
TACGCAG	20	7.0279907E-4	45.000004	1
GAGCGCG	20	7.0279907E-4	45.000004	20
TTGACGT	20	7.0279907E-4	45.000004	14
CGACTTT	20	7.0279907E-4	45.000004	17
CACGTCG	30	2.1620854E-6	45.000004	40
TATACGG	20	7.0279907E-4	45.000004	2
TCGAATG	60	0.0	45.000004	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content