##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410979
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59693074341998	31.0	31.0	34.0	30.0	34.0
2	31.70627209662781	31.0	31.0	34.0	30.0	34.0
3	31.71478348042114	31.0	31.0	34.0	30.0	34.0
4	35.5526997729811	37.0	35.0	37.0	33.0	37.0
5	33.01539738040143	37.0	35.0	37.0	28.0	37.0
6	33.99687331956134	37.0	35.0	37.0	28.0	37.0
7	35.295406821273104	37.0	35.0	37.0	32.0	37.0
8	35.14573007379939	37.0	35.0	37.0	32.0	37.0
9	37.19803931587745	39.0	37.0	39.0	34.0	39.0
10	36.69014961835033	39.0	35.0	39.0	32.0	39.0
11	36.569644677708595	39.0	35.0	39.0	32.0	39.0
12	36.91045041230817	39.0	37.0	39.0	33.0	39.0
13	36.86868185479064	39.0	37.0	39.0	33.0	39.0
14	37.849384031787515	40.0	37.0	41.0	33.0	41.0
15	37.9313030592804	40.0	37.0	41.0	33.0	41.0
16	37.969433961345956	40.0	37.0	41.0	33.0	41.0
17	37.81671812915015	39.0	37.0	41.0	33.0	41.0
18	37.741047596105886	39.0	37.0	40.0	33.0	41.0
19	37.50109129663559	39.0	36.0	40.0	33.0	41.0
20	37.13236199416515	39.0	35.0	40.0	32.0	41.0
21	37.1698042965699	39.0	35.0	40.0	32.0	41.0
22	37.164696979651026	39.0	35.0	40.0	32.0	41.0
23	36.81951389243733	39.0	35.0	40.0	32.0	41.0
24	36.57802223471272	38.0	35.0	40.0	31.0	41.0
25	36.41611858513452	38.0	35.0	40.0	31.0	41.0
26	36.67015589604335	38.0	35.0	40.0	32.0	41.0
27	36.504069550998956	38.0	35.0	40.0	31.0	41.0
28	36.55236155618657	38.0	35.0	40.0	31.0	41.0
29	36.66932373673594	39.0	35.0	40.0	32.0	41.0
30	36.3794695106076	38.0	35.0	40.0	31.0	41.0
31	36.12679723294864	38.0	35.0	40.0	30.0	41.0
32	35.80290963771871	38.0	35.0	40.0	29.0	41.0
33	35.57616082573562	38.0	35.0	40.0	26.0	41.0
34	35.290742349365786	38.0	35.0	40.0	25.0	41.0
35	35.127682922971736	38.0	35.0	40.0	23.0	41.0
36	34.911900608060265	38.0	35.0	40.0	22.0	41.0
37	34.75223308246893	38.0	35.0	40.0	21.0	41.0
38	34.69751252497086	38.0	35.0	40.0	21.0	41.0
39	34.65434243598821	38.0	35.0	40.0	21.0	41.0
40	34.5836745916458	38.0	35.0	40.0	20.0	41.0
41	34.379917222047844	38.0	34.0	40.0	18.0	41.0
42	34.46650072144806	38.0	34.0	40.0	19.0	41.0
43	34.33134783042443	38.0	34.0	40.0	18.0	41.0
44	34.263120500074216	38.0	34.0	40.0	18.0	41.0
45	34.262689821134416	38.0	34.0	40.0	18.0	41.0
46	34.14734572812723	38.0	34.0	40.0	18.0	41.0
47	33.97769715727568	38.0	33.0	40.0	18.0	41.0
48	33.92810824883996	38.0	33.0	40.0	18.0	41.0
49	33.95472274739099	38.0	33.0	40.0	18.0	41.0
50	33.843101472338006	38.0	33.0	40.0	17.0	41.0
51	32.87554351925524	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	8.0
11	13.0
12	12.0
13	15.0
14	15.0
15	41.0
16	64.0
17	120.0
18	232.0
19	522.0
20	951.0
21	1660.0
22	2470.0
23	3453.0
24	4712.0
25	6361.0
26	8014.0
27	8557.0
28	8266.0
29	8300.0
30	8849.0
31	10408.0
32	12737.0
33	16829.0
34	24396.0
35	34689.0
36	43846.0
37	56889.0
38	83329.0
39	65197.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32488034668438	21.03757126276525	21.65512106457994	17.982427325970427
2	35.9463622228873	28.335511060175822	22.045651967618785	13.672474749318091
3	23.83576776428966	28.880551074385796	34.63485968869456	12.64882147262999
4	22.391168405198318	22.61161762523146	40.737117954932	14.260096014638219
5	22.399684655420348	30.19862328732125	32.06416872881583	15.337523328442574
6	19.157183213740847	35.83540764856599	33.50365833777395	11.503750799919217
7	60.0473503512345	7.874124955289687	27.2473776032352	4.831147090240621
8	57.764022005990576	11.967521454867523	23.28756457142579	6.980891967716111
9	52.96158684506994	9.079782665294333	25.049698403081422	12.9089320865543
10	29.51805323386353	24.298565133498307	32.69388460237628	13.489497030261887
11	22.073877254068943	23.19096596176447	35.937359329795434	18.79779745437115
12	18.262733618992698	19.716822514045727	41.709673730287925	20.31077013667365
13	20.435350711350214	22.657118733560598	43.230919341377536	13.67661121371165
14	15.515391297365557	27.984884872463066	38.290277605425096	18.209446224746276
15	13.161013093126414	25.09300961849632	44.06964832753012	17.676328960847147
16	13.52356203115001	26.10790332352748	37.54960715754333	22.81892748777918
17	13.356156883928374	27.87758011966548	38.140634922952266	20.625628073453875
18	13.325741704563981	25.790612172398102	41.37972986454296	19.50391625849496
19	14.033320437297284	27.543499789526958	38.02359731275807	20.399582460417687
20	15.092498643483001	28.28246698736432	40.80646456388283	15.818569805269856
21	14.920713710432892	30.51786100992995	38.82582808367337	15.735597195963786
22	12.222765640093533	26.222994362242353	38.389066108000655	23.165173889663464
23	13.684397499628934	27.82088622533025	37.617737159319574	20.87697911572124
24	16.032935989430115	25.71712909905372	36.606249954377226	21.643684957138927
25	12.525700826562913	31.214490278092065	34.09346949600831	22.166339399336707
26	12.44029500290769	26.770954233671308	38.70173415186664	22.08701661155436
27	18.22282890366661	26.942009202416667	35.365310636309886	19.469851257606834
28	11.742935770440825	28.162266198516228	37.43305619021896	22.661741840823986
29	15.478406439258455	23.295594178777993	36.957119463524904	24.268879918438653
30	18.472233374454657	28.955007433469838	34.380345467773296	18.192413724302213
31	15.03069499901455	31.895790295854532	30.645361441825496	22.428153263305425
32	16.520308823565195	28.8131510369143	32.87978217865146	21.786757960869046
33	18.614576413880027	26.133938717063405	32.99511653880125	22.256368330255317
34	14.64405723893435	28.65839860430825	32.08728426513277	24.610259891624633
35	17.87463593030301	24.315840955377283	30.643658191781086	27.165864922538624
36	20.467955783628845	29.18372958229009	29.13749850965621	21.21081612442485
37	18.174164616683576	29.54895505609776	33.46107708666379	18.81580324055487
38	16.086466705111455	29.103676830203	32.643760386783754	22.16609607790179
39	19.040145603546655	26.56461765686325	35.018090948685945	19.37714579090416
40	17.375097024422175	24.939230471629937	34.1012557819256	23.584416722022294
41	19.123118212852724	27.927217692388172	26.90161784422075	26.04804625053835
42	20.292034386185183	24.268393275568826	31.598938145258032	23.840634192987963
43	23.1834229972821	23.295107535908162	31.49284999963502	22.028619467174725
44	19.035765817718183	24.466700245024686	32.3573710578886	24.140162879368532
45	18.693899201662372	23.063465529868925	32.31770966399743	25.924925604471277
46	21.930317607468993	25.715912491879145	31.900656724552835	20.453113176099023
47	16.111528812907714	25.32976137466878	37.65812851751549	20.90058129490801
48	17.96101503969789	24.93241747145231	33.27907265334725	23.827494835502545
49	19.52070543750411	20.690351575141307	37.59389165869789	22.195051328656696
50	19.390528469824492	20.05503930857781	34.97307648322664	25.58135573837106
51	17.828161536234212	20.04700970122561	31.204027456390715	30.920801306149464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3096.0
1	4245.5
2	5395.0
3	11453.0
4	17511.0
5	11424.0
6	5337.0
7	5128.5
8	4920.0
9	5309.0
10	5698.0
11	5827.5
12	5957.0
13	5763.0
14	5569.0
15	5256.5
16	4944.0
17	4475.5
18	4007.0
19	3816.0
20	3625.0
21	3831.0
22	4037.0
23	4199.5
24	4362.0
25	4799.5
26	5477.0
27	5717.0
28	5930.0
29	6143.0
30	6196.0
31	6249.0
32	6454.0
33	6659.0
34	7104.0
35	7549.0
36	7799.0
37	8049.0
38	8975.5
39	9902.0
40	11726.5
41	13551.0
42	16219.5
43	18888.0
44	21954.0
45	25020.0
46	39072.5
47	53125.0
48	51603.5
49	50082.0
50	47719.5
51	45357.0
52	35384.5
53	25412.0
54	20198.0
55	14984.0
56	12330.0
57	9676.0
58	8551.0
59	7426.0
60	6784.5
61	6143.0
62	5265.5
63	4388.0
64	3602.0
65	2816.0
66	2247.5
67	1679.0
68	1302.0
69	925.0
70	801.0
71	677.0
72	538.0
73	399.0
74	308.0
75	177.0
76	137.0
77	107.5
78	78.0
79	49.0
80	20.0
81	12.5
82	5.0
83	4.5
84	4.0
85	4.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.14467669919239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50692997234815	33.332290121778684
2	10.519475239948553	9.287576670253763
3	4.278476927060916	5.6661594213017485
4	2.463908894435062	4.350738462444015
5	1.6035492416571102	3.539408142209413
6	1.1319500318784215	2.998174091814806
7	0.8790173303121558	2.7162755101732126
8	0.6616520717984125	2.3366733437513396
9	0.5291607214221776	2.1023666072184097
>10	2.291398505628635	16.001617172557566
>50	0.07421569065099265	2.252221773303672
>100	0.04631505506791266	3.5366015530264465
>500	0.007812177963262378	2.587727193323314
>1k	0.005580127116615983	6.677346123694913
>5k	0.0	0.0
>10k+	5.580127116615984E-4	2.6148238131486887
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10615	2.5828570316244868	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	4741	1.153586922932802	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	4504	1.0959197428579075	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	4394	1.0691543850172394	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	3119	0.7589195555004027	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	2503	0.6090335515926605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2180	0.5304407281150618	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1708	0.4155930108351035	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	1444	0.3513561520174997	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	1287	0.31315468673581864	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	1227	0.29855540064090863	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.22555897016635884	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	927	0.22555897016635884	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	861	0.2094997554619579	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	836	0.20341671958907875	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	809	0.19684704084636928	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	798	0.19417050506230246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	766	0.18638421914501713	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA	715	0.17397482596434366	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	689	0.16764846865654937	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.16351200426299153	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.1622953970884157	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	650	0.1581589326948579	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	645	0.15694232552028206	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	543	0.13212353915893513	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448	0.10900800284199437	No Hit
ACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	416	0.10122171692470905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3338370087036077	0.0
2	0.0	0.0	0.0	2.0254076242338415	0.0
3	0.0	0.0	0.0	2.721793570961047	0.0
4	0.0	0.0	0.0	3.8138201708603114	0.0
5	0.0	0.0	0.0	7.275554225398378	0.0
6	0.0	0.0	0.0	8.78098394321851	0.0
7	0.0	0.0	0.0	10.519758917122285	0.0
8	0.0	0.0	0.0	12.865864192574316	0.0
9	0.0	0.0	0.0	13.628920212468277	0.0
10	0.0	0.0	0.0	17.11766294628193	0.0
11	0.0	0.0	0.0	20.849727115010744	0.0
12	0.0	0.0	0.0	24.48859917416705	0.0
13	0.0	0.0	0.0	25.504709486372782	0.0
14	0.0	0.0	0.0	25.91543606850958	0.0
15	0.0	0.0	0.0	26.837867628272978	0.0
16	0.0	0.0	0.0	28.545984101377442	0.0
17	0.0	0.0	0.0	30.605700047934324	0.0
18	0.0	0.0	0.0	32.66249613727222	0.0
19	0.0	0.0	0.0	34.438742612152936	0.0
20	0.0	0.0	0.0	35.71009710958467	0.0
21	0.0	0.0	0.0	37.116495003394334	0.0
22	0.0	0.0	0.0	38.5919961847199	0.0
23	0.0	0.0	0.0	39.945350005718055	0.0
24	0.0	0.0	0.0	40.8943036018872	0.0
25	0.0	0.0	0.0	41.672688872180814	0.0
26	0.0	0.0	0.0	42.32625024636295	0.0
27	0.0	0.0	0.0	42.89221590397563	0.0
28	0.0	0.0	0.0	43.43895916823001	0.0
29	2.4332143491516598E-4	0.0	0.0	44.01903746906776	0.0
30	2.4332143491516598E-4	0.0	0.0	44.55823776883977	0.0
31	2.4332143491516598E-4	0.0	0.0	45.060453210504676	0.0
32	2.4332143491516598E-4	0.0	0.0	45.5582888663411	0.0
33	2.4332143491516598E-4	0.0	0.0	45.9721786271318	0.0
34	2.4332143491516598E-4	0.0	0.0	46.37755213770047	0.0
35	4.8664286983033197E-4	0.0	0.0	46.765406504955244	0.0
36	4.8664286983033197E-4	0.0	0.0	47.15764065803849	0.0
37	4.8664286983033197E-4	0.0	0.0	47.54354845381394	0.0
38	4.8664286983033197E-4	0.0	0.0	47.93334939254804	0.0
39	4.8664286983033197E-4	0.0	0.0	48.29005861613367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCG	30	2.1620854E-6	45.000004	31
TCGTTAG	20	7.0279907E-4	45.000004	1
TTGTGCG	20	7.0279907E-4	45.000004	1
CATGCGG	30	2.1620854E-6	45.000004	2
GCGAAAG	40	6.7975634E-9	45.000004	1
CGGCAGT	20	7.0279907E-4	45.000004	35
GACCGAT	45	3.8380676E-10	45.000004	9
GCCGGAT	30	2.1620854E-6	45.000004	1
TGAGTCG	20	7.0279907E-4	45.000004	1
CGTTCAC	20	7.0279907E-4	45.000004	18
CGTTAGG	35	1.2093733E-7	45.000004	2
CGGGGTA	20	7.0279907E-4	45.000004	6
GGGACGT	20	7.0279907E-4	45.000004	8
TACGCAG	20	7.0279907E-4	45.000004	1
GAGCGCG	20	7.0279907E-4	45.000004	20
TTGACGT	20	7.0279907E-4	45.000004	14
CGACTTT	20	7.0279907E-4	45.000004	17
CACGTCG	30	2.1620854E-6	45.000004	40
TATACGG	20	7.0279907E-4	45.000004	2
TCGAATG	60	0.0	45.000004	44
>>END_MODULE