FastQCFastQC Report
Sat 18 Jun 2016
SRR3550124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432065
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120062.7787485679238078No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC38970.9019476236214458No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC37720.8730167914549894No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG35690.8260331200166641No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC31320.7248909307627325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30970.7167902977561247No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC26960.6239801881661324No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC22730.5260782521148438No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19130.4427574554754493No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT16020.3707775450453057No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT14040.3249511068936387No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.27125548239269553No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10460.24209320356890748No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG9920.22959508407299828No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.2210315577517272No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT9080.21015356485713957No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC8650.20020135859187851No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.19487808547325056No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8340.19302651221459735No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.1907120456412808No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.18631455915197945No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT7920.18330575260666798No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC7570.17520511960006016No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTA6460.14951454063624686No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTC6450.1492830939789152No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC5820.13470195456702116No Hit
TTGTGTGGGATTTCTATTAATTTTAAAAGGTTGATATTGGTTTTTATAGTT5230.12104660178445374No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG4610.10669690902989135No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT4450.10299376251258492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG302.1623455E-645.0000041
GCGAAAG302.1623455E-645.0000041
TCTAGCG302.1623455E-645.0000041
TGCGAGG453.8380676E-1045.0000042
TACTCAC302.1623455E-645.00000430
GTGCGAG302.1623455E-645.0000041
GTTGAGT253.886751E-545.010
AAGTACG253.886751E-545.01
AGACTCG207.028394E-445.01
CGAACCC253.886751E-545.034
GTTTCGT207.028394E-445.026
CGGCCAT253.886751E-545.031
TACGGAG253.886751E-545.04
GCGCATG207.028394E-445.01
ACGGGTA351.2095734E-745.05
CCGTCGA207.028394E-445.041
TTGAGCG207.028394E-445.01
GTTACGG207.028394E-445.02
CTAACGG253.886751E-545.02
CGCTAGG207.028394E-445.02
Produced by FastQC (version 0.11.5)