##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432065
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739323944313934	31.0	31.0	34.0	30.0	34.0
2	31.845074236515337	33.0	31.0	34.0	30.0	34.0
3	31.856514644787243	33.0	31.0	34.0	30.0	34.0
4	35.63396248249685	37.0	35.0	37.0	33.0	37.0
5	33.07307928205247	37.0	35.0	37.0	28.0	37.0
6	34.09754087926585	37.0	35.0	37.0	28.0	37.0
7	35.36204506266418	37.0	35.0	37.0	32.0	37.0
8	35.224197748024025	37.0	35.0	37.0	32.0	37.0
9	37.32356242694965	39.0	37.0	39.0	34.0	39.0
10	36.882654230266276	39.0	37.0	39.0	32.0	39.0
11	36.75010241514587	39.0	37.0	39.0	32.0	39.0
12	37.109638596044576	39.0	37.0	39.0	34.0	39.0
13	37.080941525002025	39.0	37.0	39.0	33.0	39.0
14	38.104417159455174	40.0	37.0	41.0	33.0	41.0
15	38.20364991378612	40.0	38.0	41.0	33.0	41.0
16	38.223440917454546	40.0	38.0	41.0	33.0	41.0
17	38.08133035538634	40.0	37.0	41.0	33.0	41.0
18	37.97387661578698	39.0	37.0	41.0	33.0	41.0
19	37.7361091502436	39.0	37.0	41.0	33.0	41.0
20	37.39271868816034	39.0	35.0	41.0	33.0	41.0
21	37.393959242243646	39.0	35.0	40.0	33.0	41.0
22	37.387485679238075	39.0	35.0	40.0	33.0	41.0
23	37.06653397058313	39.0	35.0	40.0	32.0	41.0
24	36.85614895906866	39.0	35.0	40.0	32.0	41.0
25	36.6674643861456	38.0	35.0	40.0	31.0	41.0
26	36.92700866767732	39.0	35.0	40.0	32.0	41.0
27	36.793375996667166	39.0	35.0	40.0	32.0	41.0
28	36.85077939661856	39.0	35.0	40.0	32.0	41.0
29	36.95551132352771	39.0	35.0	40.0	33.0	41.0
30	36.693502135095414	39.0	35.0	40.0	32.0	41.0
31	36.50895582840545	39.0	35.0	40.0	31.0	41.0
32	36.1643248122389	39.0	35.0	40.0	30.0	41.0
33	35.95094719544513	39.0	35.0	40.0	29.0	41.0
34	35.720391607744205	39.0	35.0	40.0	27.0	41.0
35	35.497402011271454	39.0	35.0	40.0	24.0	41.0
36	35.298637936421606	39.0	35.0	40.0	23.0	41.0
37	35.166183328897276	39.0	35.0	40.0	23.0	41.0
38	35.06074317521669	39.0	35.0	40.0	22.0	41.0
39	34.990617152511774	39.0	35.0	40.0	21.0	41.0
40	34.8778563410598	38.0	35.0	40.0	21.0	41.0
41	34.76875007232708	38.0	35.0	40.0	21.0	41.0
42	34.776214227026024	38.0	35.0	40.0	20.0	41.0
43	34.68031430456065	38.0	35.0	40.0	18.0	41.0
44	34.649089836020046	38.0	35.0	40.0	18.0	41.0
45	34.65760012961013	38.0	35.0	40.0	20.0	41.0
46	34.53113767604411	38.0	35.0	40.0	20.0	41.0
47	34.4205478342379	38.0	34.0	40.0	18.0	41.0
48	34.37065256385035	38.0	34.0	40.0	18.0	41.0
49	34.385208244129934	38.0	34.0	40.0	18.0	41.0
50	34.29326143057179	38.0	34.0	40.0	18.0	41.0
51	33.40324025320265	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	8.0
11	8.0
12	17.0
13	24.0
14	18.0
15	43.0
16	76.0
17	110.0
18	221.0
19	440.0
20	821.0
21	1512.0
22	2382.0
23	3442.0
24	4482.0
25	5931.0
26	7932.0
27	8464.0
28	8360.0
29	7975.0
30	8556.0
31	9655.0
32	11957.0
33	15751.0
34	23641.0
35	35245.0
36	44340.0
37	56891.0
38	89059.0
39	84680.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.38921227130177	19.764850196151045	21.700670038072975	19.14526749447421
2	35.25395484475716	28.18904562970849	22.561188710032056	13.995810815502296
3	25.58041035492345	28.57648733408168	34.2524851584831	11.590617152511774
4	22.404499323018527	24.06003726291183	39.385740571441794	14.149722842627845
5	22.12051427447259	31.19114022195734	31.94079594505456	14.747549558515502
6	18.844849733257725	35.66037517503153	33.67340562183931	11.821369469871431
7	59.71439482485275	9.01669887632648	26.47888627868492	4.790020020135859
8	58.015113466723754	12.477057850092	23.13101038038258	6.376818302801662
9	52.87676622730376	11.229328920417068	24.685174684364622	11.20873016791455
10	27.1646627243586	30.223230300996377	31.01246340249731	11.59964357214771
11	20.055084304444932	23.84710633816671	38.845775519887056	17.2520338375013
12	17.69178248643144	21.28800064805064	42.44662261465289	18.573594250865032
13	19.547753231573953	23.186557578142178	43.73462326270353	13.531065927580341
14	15.078749725157095	29.078032240519363	38.72750627799058	17.11571175633296
15	12.257414972284263	26.60919074676264	43.921863608484834	17.21153067246826
16	13.078587712496962	28.353141309756637	38.06626317799405	20.502007799752352
17	13.280409197690163	28.301297258514346	39.1971115457165	19.221181998078993
18	12.966336083691111	26.873271382778054	41.260458495828175	18.89993403770266
19	13.807413236434332	27.437538333352617	39.0545404048002	19.700508025412844
20	15.09911703100228	29.441634939187388	40.56773865043454	14.891509379375789
21	14.786201150289887	29.798988578107462	39.93936097577911	15.475449295823546
22	12.113455151423976	27.073009848055268	39.62019603531876	21.193338965201995
23	13.392197933181349	27.799752352076656	39.2153958316457	19.592653883096293
24	15.126427736567413	27.493085531112214	37.263143277053224	20.117343455267147
25	12.855473134829252	31.690602108479048	35.47406061587955	19.979864140812147
26	12.325460289539768	28.354298543043292	40.21223658477312	19.108004582643815
27	15.834423061344937	28.037910962470924	39.19456563248585	16.93310034369829
28	11.581822179533171	29.45251293208198	39.1554511473968	19.810213740988043
29	15.870991633203339	24.896254035851086	39.934500595975145	19.298253734970434
30	15.15373844213255	28.951893812273617	37.730434078205825	18.163933667388008
31	14.788978510177866	31.135361577540415	33.05127700693183	21.02438290534989
32	17.569347204702996	31.055743927418327	32.84112344207469	18.533785425803988
33	17.806348581810607	26.919329267587056	31.911402219573443	23.362919931028898
34	15.398146112274775	27.459062872484463	33.67340562183931	23.469385393401456
35	16.511404534040018	27.014453843750363	34.66399731521877	21.810144306990846
36	17.20389293277632	29.13033918507632	34.53670165368637	19.129066228460996
37	17.585548470716212	29.060210847904827	35.487947415319454	17.866293266059504
38	18.253272077118027	31.119391758184534	29.165981970305392	21.461354194392047
39	22.637103213636838	24.522930577575135	30.12347679168644	22.71648941710159
40	17.808431601726593	25.711872056287827	32.82793098260679	23.6517653593788
41	17.667480587411617	28.410540080774883	30.657424230150554	23.264555101662946
42	18.992744147292655	27.680557323550854	34.57396456551676	18.75273396363973
43	22.159628759561638	25.20801268327682	33.66692511543402	18.965433441727516
44	18.6742735468043	24.824737018735608	33.022577621422705	23.47841181303739
45	19.0552347447722	23.474708666520083	31.6792612222698	25.79079536643792
46	21.06534896369759	26.1606471248539	32.45900501082013	20.314998900628378
47	15.951997963269415	26.168747757860505	37.512642773656744	20.366611505213335
48	17.481166028259636	26.260863527478506	33.50398666867254	22.75398377558932
49	19.595894136298934	22.968997720250425	36.52181963361994	20.913288509830696
50	18.80874405471399	22.31909550646315	33.94952148403597	24.922638954786898
51	17.422147130640063	21.910823602930115	31.420735306030345	29.246293960399477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4589.0
1	5626.5
2	6664.0
3	13172.0
4	19680.0
5	12742.0
6	5804.0
7	5814.5
8	5825.0
9	6144.0
10	6463.0
11	6422.5
12	6382.0
13	6398.5
14	6415.0
15	5850.0
16	5285.0
17	5043.0
18	4801.0
19	5091.5
20	5382.0
21	5582.5
22	5783.0
23	6089.0
24	6395.0
25	7454.5
26	7950.5
27	7387.0
28	7933.5
29	8480.0
30	8477.0
31	8474.0
32	8636.0
33	8798.0
34	9333.0
35	9868.0
36	9994.5
37	10121.0
38	10751.0
39	11381.0
40	13315.0
41	15249.0
42	18149.5
43	21050.0
44	22772.5
45	24495.0
46	36486.5
47	48478.0
48	47468.0
49	46458.0
50	44139.5
51	41821.0
52	32510.0
53	23199.0
54	18468.5
55	13738.0
56	11444.5
57	9151.0
58	8191.5
59	7232.0
60	6803.0
61	6374.0
62	5470.0
63	4566.0
64	3863.0
65	3160.0
66	2436.0
67	1712.0
68	1406.0
69	1100.0
70	863.5
71	627.0
72	570.5
73	514.0
74	393.0
75	218.5
76	165.0
77	144.5
78	124.0
79	90.0
80	56.0
81	33.0
82	10.0
83	12.5
84	15.0
85	10.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.54424329990635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12175200429527	30.05210347077469
2	10.20116802297773	8.271972765336676
3	4.337123066137646	5.275361184453617
4	2.4942477060612944	4.045095433791297
5	1.7238026602035141	3.4945137228158534
6	1.2931959044916403	3.1458989631690915
7	1.0174911619589562	2.8877386458177843
8	0.8381724067793609	2.718645279018439
9	0.6888523882827303	2.513609894543092
>10	3.127627973390959	19.17684314574842
>50	0.09070469649846305	2.574521537357124
>100	0.05026311207239672	3.53466745507406
>500	0.008088316885213264	2.5696025170419112
>1k	0.006932843044468513	6.9271517991322185
>5k	0.0	0.0
>10k+	5.77736920372376E-4	2.8122741859257236
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12006	2.7787485679238078	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	3897	0.9019476236214458	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	3772	0.8730167914549894	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	3569	0.8260331200166641	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	3132	0.7248909307627325	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3097	0.7167902977561247	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2696	0.6239801881661324	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2273	0.5260782521148438	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1913	0.4427574554754493	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	1602	0.3707775450453057	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT	1404	0.3249511068936387	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.27125548239269553	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1046	0.24209320356890748	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	992	0.22959508407299828	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	955	0.2210315577517272	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	908	0.21015356485713957	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	865	0.20020135859187851	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.19487808547325056	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	834	0.19302651221459735	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.1907120456412808	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.18631455915197945	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	792	0.18330575260666798	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	757	0.17520511960006016	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTA	646	0.14951454063624686	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTC	645	0.1492830939789152	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	582	0.13470195456702116	No Hit
TTGTGTGGGATTTCTATTAATTTTAAAAGGTTGATATTGGTTTTTATAGTT	523	0.12104660178445374	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	461	0.10669690902989135	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT	445	0.10299376251258492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3885989376598428	0.0
2	0.0	0.0	0.0	1.9672965873190376	0.0
3	0.0	0.0	0.0	2.5641975165773667	0.0
4	0.0	0.0	0.0	3.475865899806742	0.0
5	0.0	0.0	0.0	6.332149097936653	0.0
6	0.0	0.0	0.0	7.590987467163505	0.0
7	0.0	0.0	0.0	9.044703921863608	0.0
8	0.0	0.0	0.0	10.90738662006874	0.0
9	0.0	0.0	0.0	11.593163065742424	0.0
10	0.0	0.0	0.0	14.26266881140569	0.0
11	0.0	0.0	0.0	17.52213208660734	0.0
12	0.0	0.0	0.0	20.83112494647796	0.0
13	0.0	0.0	0.0	21.789777001145662	0.0
14	0.0	0.0	0.0	22.192725631560066	0.0
15	0.0	0.0	0.0	23.031719764387304	0.0
16	0.0	0.0	0.0	24.49006515223404	0.0
17	0.0	0.0	0.0	26.283313853239676	0.0
18	0.0	0.0	0.0	28.032587689352297	0.0
19	0.0	0.0	0.0	29.553192228021246	0.0
20	0.0	0.0	0.0	30.687975188918333	0.0
21	0.0	0.0	0.0	31.884091514008308	0.0
22	0.0	0.0	0.0	33.16121416916436	0.0
23	0.0	0.0	0.0	34.24693043870714	0.0
24	0.0	0.0	0.0	35.06949185886383	0.0
25	0.0	0.0	0.0	35.683751287422034	0.0
26	0.0	0.0	0.0	36.224873572263434	0.0
27	0.0	0.0	0.0	36.74562855125965	0.0
28	0.0	0.0	0.0	37.239998611320054	0.0
29	0.0	0.0	0.0	37.75473597722565	0.0
30	0.0	0.0	0.0	38.28197146262715	0.0
31	0.0	0.0	0.0	38.71269369192135	0.0
32	0.0	0.0	0.0	39.15707127399813	0.0
33	0.0	0.0	0.0	39.56696330413248	0.0
34	0.0	0.0	0.0	39.95926538830963	0.0
35	0.0	0.0	0.0	40.3714718850173	0.0
36	0.0	0.0	0.0	40.76007082267714	0.0
37	0.0	0.0	0.0	41.14427227384768	0.0
38	0.0	0.0	0.0	41.52477057850092	0.0
39	0.0	0.0	0.0	41.88420723733697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1623455E-6	45.000004	1
GCGAAAG	30	2.1623455E-6	45.000004	1
TCTAGCG	30	2.1623455E-6	45.000004	1
TGCGAGG	45	3.8380676E-10	45.000004	2
TACTCAC	30	2.1623455E-6	45.000004	30
GTGCGAG	30	2.1623455E-6	45.000004	1
GTTGAGT	25	3.886751E-5	45.0	10
AAGTACG	25	3.886751E-5	45.0	1
AGACTCG	20	7.028394E-4	45.0	1
CGAACCC	25	3.886751E-5	45.0	34
GTTTCGT	20	7.028394E-4	45.0	26
CGGCCAT	25	3.886751E-5	45.0	31
TACGGAG	25	3.886751E-5	45.0	4
GCGCATG	20	7.028394E-4	45.0	1
ACGGGTA	35	1.2095734E-7	45.0	5
CCGTCGA	20	7.028394E-4	45.0	41
TTGAGCG	20	7.028394E-4	45.0	1
GTTACGG	20	7.028394E-4	45.0	2
CTAACGG	25	3.886751E-5	45.0	2
CGCTAGG	20	7.028394E-4	45.0	2
>>END_MODULE