FastQCFastQC Report
Sat 18 Jun 2016
SRR3550123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434449
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114612.638054178971525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44821.031651586262139No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG43891.0102451611121213No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC40900.9414223533717422No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC40660.9358981146233505TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC36040.8295565187168114No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC29770.6852357814150798No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC25880.5956970783682319TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT19120.4400976869552008TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14550.3349069741212432No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC14460.33283538459059636TruSeq Adapter, Index 20 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14400.33145432490349847No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT13030.29992012871476287No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12220.28127582293894104No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT11110.25572621872762974No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10580.24352685815826483No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG9380.2159056644163066No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC9320.21452460472920873TruSeq Adapter, Index 22 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.20301577400339282No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.18874482390338107No Hit
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT8190.18851464728886475No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.1829904085404731No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC7170.16503663260820026No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA6820.15698045110012915No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC6500.14961479943560696No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.12935925735817094No Hit
ACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG4570.1051907128339575No Hit
GCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG4520.10403982976137591TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG650.045.0000042
CGTTATT3100.045.0000041
TGATCCG253.8867864E-545.04
AAGTACG253.8867864E-545.01
CTGTCGG207.028437E-445.02
TCGCAGG253.8867864E-545.02
TCACCGG207.028437E-445.02
CATGCGG207.028437E-445.02
CAGCCGC207.028437E-445.016
GCGAAAG750.045.01
GGCACCG207.028437E-445.08
TGAACTC253.8867864E-545.010
GGATCGA207.028437E-445.08
TTCGTAG406.7975634E-945.01
ACGGGTA207.028437E-445.05
ACGGCCA207.028437E-445.030
GTGTATC207.028437E-445.09
GTACTCG207.028437E-445.01
GACGGTC351.2095916E-745.09
TTGCGAG351.2095916E-745.01
Produced by FastQC (version 0.11.5)