##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434449
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.628713611954453	31.0	31.0	34.0	30.0	34.0
2	31.73425189147633	31.0	31.0	34.0	30.0	34.0
3	31.756033504508007	31.0	31.0	34.0	30.0	34.0
4	35.58161487309212	37.0	35.0	37.0	33.0	37.0
5	32.955435505663495	37.0	35.0	37.0	28.0	37.0
6	33.99435837117821	37.0	35.0	37.0	27.0	37.0
7	35.2806773637412	37.0	35.0	37.0	32.0	37.0
8	35.13738321414021	37.0	35.0	37.0	32.0	37.0
9	37.25053573607029	39.0	37.0	39.0	34.0	39.0
10	36.83089844837944	39.0	37.0	39.0	32.0	39.0
11	36.648317754212805	39.0	37.0	39.0	32.0	39.0
12	37.0329797053279	39.0	37.0	39.0	33.0	39.0
13	36.967669392725035	39.0	37.0	39.0	33.0	39.0
14	37.951023019963216	40.0	37.0	41.0	33.0	41.0
15	38.06301084822384	40.0	37.0	41.0	33.0	41.0
16	38.08517685620176	40.0	37.0	41.0	33.0	41.0
17	37.973529689330626	40.0	37.0	41.0	33.0	41.0
18	37.873835594051315	39.0	37.0	40.0	33.0	41.0
19	37.64324005809658	39.0	36.0	40.0	33.0	41.0
20	37.34059924179823	39.0	35.0	40.0	33.0	41.0
21	37.317466492039344	39.0	35.0	40.0	33.0	41.0
22	37.285946106447476	39.0	35.0	40.0	33.0	41.0
23	36.963351279436715	39.0	35.0	40.0	32.0	41.0
24	36.68315498481985	38.0	35.0	40.0	31.0	41.0
25	36.48413968037676	38.0	35.0	40.0	31.0	41.0
26	36.75369491010452	38.0	35.0	40.0	32.0	41.0
27	36.65495144424317	38.0	35.0	40.0	32.0	41.0
28	36.68610124548566	38.0	35.0	40.0	32.0	41.0
29	36.735773358898285	39.0	35.0	40.0	32.0	41.0
30	36.49119459361168	38.0	35.0	40.0	31.0	41.0
31	36.34240612822219	38.0	35.0	40.0	31.0	41.0
32	36.09985291714332	38.0	35.0	40.0	30.0	41.0
33	35.952553694449755	39.0	35.0	40.0	30.0	41.0
34	35.73949531475501	39.0	35.0	40.0	27.0	41.0
35	35.5951653703887	39.0	35.0	40.0	26.0	41.0
36	35.40086178124475	39.0	35.0	40.0	25.0	41.0
37	35.35233824913856	39.0	35.0	40.0	25.0	41.0
38	35.260622075318395	39.0	35.0	40.0	24.0	41.0
39	35.235049453445626	39.0	35.0	40.0	24.0	41.0
40	35.163360946854525	39.0	35.0	40.0	23.0	41.0
41	34.98238228192492	38.0	35.0	40.0	23.0	41.0
42	35.03605716666398	39.0	35.0	40.0	23.0	41.0
43	34.902775699794454	38.0	35.0	40.0	21.0	41.0
44	34.83790042099303	38.0	35.0	40.0	21.0	41.0
45	34.84059809091516	38.0	35.0	40.0	21.0	41.0
46	34.737223471569735	38.0	35.0	40.0	20.0	41.0
47	34.633623279142085	38.0	35.0	40.0	20.0	41.0
48	34.59693082502204	38.0	34.0	40.0	20.0	41.0
49	34.62959518838805	38.0	35.0	40.0	20.0	41.0
50	34.50339625594719	38.0	34.0	40.0	20.0	41.0
51	33.63738436502328	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	5.0
10	4.0
11	13.0
12	13.0
13	20.0
14	15.0
15	42.0
16	54.0
17	108.0
18	190.0
19	434.0
20	787.0
21	1294.0
22	2041.0
23	3053.0
24	4149.0
25	5807.0
26	7189.0
27	8098.0
28	8164.0
29	8071.0
30	8605.0
31	10250.0
32	13055.0
33	16912.0
34	25013.0
35	37887.0
36	47142.0
37	59180.0
38	89293.0
39	77540.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.03794921843531	22.882777955525274	21.78828815350018	18.29098467253924
2	33.660337577022844	29.489076968758127	22.8436479310575	14.006937523161522
3	23.876910753621253	28.644098616868725	34.81444312220767	12.664547507302354
4	22.38996982384584	25.16129626262231	38.587268010744644	13.86146590278721
5	20.76492292536063	32.683698201630115	31.138062235153036	15.413316637856228
6	19.20064265310773	35.85806389242466	32.545822409534836	12.395471044932776
7	59.009457957090476	9.837288151198415	25.719244376209865	5.434009515501244
8	56.24756875950917	12.970222051380023	22.973467541644705	7.8087416474661016
9	51.08746941528235	11.889312669611392	25.253827261657868	11.769390653448392
10	26.414377752049145	29.839635952666484	31.502661992546884	12.243324302737491
11	18.598730806147557	24.526469159786306	38.29402300385085	18.580777030215284
12	17.042046362173696	21.67319984624202	42.973053223738575	18.31170056784571
13	18.32297922195701	25.143112310075523	43.43202539308411	13.101883074883357
14	13.160347934970504	29.741350538268012	39.13739011943865	17.96091140732284
15	11.113387302076884	27.504033845169403	44.61329177878186	16.76928707397186
16	11.773073479280653	28.94516962865607	37.929883599686036	21.351873292377242
17	11.262311571668942	29.62534152455179	38.35801210268639	20.754334801092877
18	11.275891991925405	28.97371152885609	41.50959030864382	18.240806170574682
19	12.431378596797323	29.319666980474118	38.061774799803885	20.18717962292467
20	13.551418003033728	30.82847468862858	40.65563506878828	14.964472239549407
21	13.830392059827506	31.288597741046704	39.19930762874353	15.681702570382255
22	10.723928470315274	29.137827455006228	37.48702379335664	22.65122028132186
23	12.508027409431257	29.222762625762748	37.82054970779079	20.44866025701521
24	15.536000773393424	27.46927717637743	36.07419973345548	20.92052231677366
25	11.27612216853992	33.27985563322737	33.90179284565047	21.542229352582236
26	11.078170280055886	31.91237636638593	36.61994848647367	20.389504867084515
27	15.408252752336868	29.434064757888727	35.13761108898858	20.02007140078582
28	10.144113578348666	31.538339367796908	36.33222771832828	21.98531933552615
29	13.352085054862597	28.898443775909254	34.11700798022322	23.632463189004923
30	13.385460663967464	31.53419618873562	32.16418958266678	22.91615356463014
31	12.734521198115315	34.12184168912807	30.625919267854222	22.517717844902393
32	13.099581308738195	32.58702402353325	30.243135557913586	24.070259109814963
33	15.480298032680476	27.786920904409957	29.417031688414518	27.315749374495052
34	12.517464650626426	29.833651360689057	34.47585332225416	23.173030666430353
35	13.087842301397862	28.288245570826497	31.95726080621661	26.66665132155903
36	16.597114966313654	31.643760257245386	29.419793807788718	22.339330968652245
37	13.651775006962843	29.66723366839376	34.233247170553966	22.447744154089435
38	14.167370623479394	30.296306355866857	29.09363354501909	26.442689475634655
39	18.359347127050583	24.95989172492053	32.4479973483654	24.23276379966348
40	16.52898268841682	24.66917866078642	36.02425140810544	22.777587242691315
41	16.608163443810437	28.076943438700514	32.708096922768846	22.606796194720207
42	17.957919111334128	26.672175560307426	34.280893729758844	21.089011598599605
43	20.91729984417043	26.02031538799721	30.40287812838791	22.659506639444444
44	16.47397047754742	25.588734235779114	30.617863086346155	27.31943220032731
45	17.146316368549588	23.34750454023372	30.937808580523836	28.56837051069285
46	19.532327154625744	25.665613225027563	33.09893681421755	21.703122806129144
47	13.881491268250128	25.47870981404031	38.984322670785296	21.655476246924266
48	15.546128544432142	24.917769404464046	34.37940932077183	25.156692730331986
49	17.896461955258268	21.596781210222606	38.30645254103474	22.20030429348439
50	18.06587194354228	20.64223878982343	34.72812689176405	26.563762374870237
51	16.793455618496072	19.59447484054515	32.166030995582915	31.446038545375863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5926.0
1	6762.5
2	7599.0
3	13319.0
4	19039.0
5	12367.5
6	5696.0
7	5554.0
8	5412.0
9	5592.5
10	5773.0
11	6063.0
12	6353.0
13	6306.5
14	6260.0
15	5868.5
16	5477.0
17	5353.0
18	5229.0
19	4937.5
20	4646.0
21	4904.0
22	5162.0
23	5573.5
24	5985.0
25	6863.0
26	7639.5
27	7538.0
28	7783.0
29	8028.0
30	7964.0
31	7900.0
32	8114.5
33	8329.0
34	8422.5
35	8516.0
36	9047.5
37	9579.0
38	10333.5
39	11088.0
40	12606.0
41	14124.0
42	17240.0
43	20356.0
44	23832.0
45	27308.0
46	40847.0
47	54386.0
48	53829.5
49	53273.0
50	49571.0
51	45869.0
52	34800.5
53	23732.0
54	17932.5
55	12133.0
56	9827.0
57	7521.0
58	6587.0
59	5653.0
60	5161.5
61	4670.0
62	3871.5
63	3073.0
64	2554.0
65	2035.0
66	1588.5
67	1142.0
68	957.5
69	773.0
70	630.5
71	488.0
72	390.0
73	292.0
74	258.5
75	138.0
76	51.0
77	46.0
78	41.0
79	26.5
80	12.0
81	12.5
82	13.0
83	6.5
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24986524141164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3067607700006	26.21110333955512
2	10.962589421732542	7.947847784694588
3	4.6737352837110535	5.082668226256695
4	2.6217931306429767	3.8015859070666664
5	1.6881889971865442	3.059831182502305
6	1.2951413734101644	2.8169220152817154
7	0.9354076421068603	2.3735880680512205
8	0.790581366182905	2.2926774389201134
9	0.6516275336287183	2.125926925347103
>10	3.855413812377411	22.629469899493973
>50	0.11873735427648702	3.0269846416238386
>100	0.0832452103351458	5.437811822193798
>500	0.00645311708024386	1.8239102975843178
>1k	0.00967967562036579	8.688662329508055
>5k	0.0	0.0
>10k+	6.453117080243861E-4	2.6810101219204645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11461	2.638054178971525	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4482	1.031651586262139	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	4389	1.0102451611121213	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	4090	0.9414223533717422	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	4066	0.9358981146233505	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	3604	0.8295565187168114	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	2977	0.6852357814150798	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	2588	0.5956970783682319	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1912	0.4400976869552008	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1455	0.3349069741212432	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1446	0.33283538459059636	TruSeq Adapter, Index 20 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1440	0.33145432490349847	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	1303	0.29992012871476287	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1222	0.28127582293894104	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	1111	0.25572621872762974	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1058	0.24352685815826483	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	938	0.2159056644163066	No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	932	0.21452460472920873	TruSeq Adapter, Index 22 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	882	0.20301577400339282	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	820	0.18874482390338107	No Hit
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	819	0.18851464728886475	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.1829904085404731	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	717	0.16503663260820026	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	682	0.15698045110012915	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	650	0.14961479943560696	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.12935925735817094	No Hit
ACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG	457	0.1051907128339575	No Hit
GCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG	452	0.10403982976137591	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6035322903263676E-4	0.0	0.0	0.4748543557471648	0.0
2	4.6035322903263676E-4	0.0	0.0	2.7002018648909307	0.0
3	4.6035322903263676E-4	0.0	0.0	3.479810058257701	0.0
4	4.6035322903263676E-4	0.0	0.0	4.689848520769987	0.0
5	4.6035322903263676E-4	0.0	0.0	8.282675296755201	0.0
6	4.6035322903263676E-4	0.0	0.0	9.821405964796789	0.0
7	4.6035322903263676E-4	0.0	0.0	11.662818880927336	0.0
8	4.6035322903263676E-4	0.0	0.0	14.038701895964774	0.0
9	4.6035322903263676E-4	0.0	0.0	14.885061307541276	0.0
10	4.6035322903263676E-4	0.0	0.0	18.102239848635858	0.0
11	4.6035322903263676E-4	0.0	0.0	21.918798293930934	0.0
12	6.905298435489551E-4	0.0	0.0	25.4319839612935	0.0
13	6.905298435489551E-4	0.0	0.0	26.47422367182339	0.0
14	6.905298435489551E-4	0.0	0.0	26.915932595080204	0.0
15	6.905298435489551E-4	0.0	0.0	27.913978395622962	0.0
16	6.905298435489551E-4	0.0	0.0	29.568718077380773	0.0
17	6.905298435489551E-4	0.0	0.0	31.69278787613736	0.0
18	6.905298435489551E-4	0.0	0.0	33.74596327762292	0.0
19	6.905298435489551E-4	0.0	0.0	35.489090779354996	0.0
20	6.905298435489551E-4	0.0	0.0	36.780611763406064	0.0
21	6.905298435489551E-4	0.0	0.0	38.21115942262498	0.0
22	6.905298435489551E-4	0.0	0.0	39.74965991405205	0.0
23	6.905298435489551E-4	0.0	0.0	41.039339485186986	0.0
24	6.905298435489551E-4	0.0	0.0	41.9225271550861	0.0
25	9.207064580652735E-4	0.0	0.0	42.674053801481875	0.0
26	9.207064580652735E-4	0.0	0.0	43.30358684218401	0.0
27	9.207064580652735E-4	0.0	0.0	43.881330144619966	0.0
28	9.207064580652735E-4	0.0	0.0	44.41050618139298	0.0
29	9.207064580652735E-4	0.0	0.0	44.94267451415471	0.0
30	9.207064580652735E-4	0.0	0.0	45.53169647070197	0.0
31	9.207064580652735E-4	0.0	0.0	45.98859705051686	0.0
32	9.207064580652735E-4	0.0	0.0	46.42892491408658	0.0
33	9.207064580652735E-4	0.0	0.0	46.84232211375789	0.0
34	9.207064580652735E-4	0.0	0.0	47.24121818671467	0.0
35	9.207064580652735E-4	0.0	0.0	47.64494796857629	0.0
36	9.207064580652735E-4	0.0	0.0	48.037629272941125	0.0
37	9.207064580652735E-4	0.0	0.0	48.43767622897049	0.0
38	9.207064580652735E-4	0.0	0.0	48.812403757403054	0.0
39	9.207064580652735E-4	0.0	0.0	49.17078874620496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	65	0.0	45.000004	2
CGTTATT	310	0.0	45.000004	1
TGATCCG	25	3.8867864E-5	45.0	4
AAGTACG	25	3.8867864E-5	45.0	1
CTGTCGG	20	7.028437E-4	45.0	2
TCGCAGG	25	3.8867864E-5	45.0	2
TCACCGG	20	7.028437E-4	45.0	2
CATGCGG	20	7.028437E-4	45.0	2
CAGCCGC	20	7.028437E-4	45.0	16
GCGAAAG	75	0.0	45.0	1
GGCACCG	20	7.028437E-4	45.0	8
TGAACTC	25	3.8867864E-5	45.0	10
GGATCGA	20	7.028437E-4	45.0	8
TTCGTAG	40	6.7975634E-9	45.0	1
ACGGGTA	20	7.028437E-4	45.0	5
ACGGCCA	20	7.028437E-4	45.0	30
GTGTATC	20	7.028437E-4	45.0	9
GTACTCG	20	7.028437E-4	45.0	1
GACGGTC	35	1.2095916E-7	45.0	9
TTGCGAG	35	1.2095916E-7	45.0	1
>>END_MODULE