SRR3550118_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369727
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8850	2.393658023352365	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4403	1.1908786753469451	No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	2454	0.6637329705431304	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	2263	0.6120732324120229	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	2113	0.5715027574399489	RNA PCR Primer, Index 40 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2008	0.5431034249594971	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	1851	0.500639661155393	Illumina PCR Primer Index 8 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1754	0.4744040873401185	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1547	0.4184168318786564	No Hit
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1052	0.28453426447081226	RNA PCR Primer, Index 40 (95% over 24bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.2842637946376651	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.21718727601716942	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.20285237486036994	No Hit
ACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	734	0.19852485753001542	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	656	0.17742821054453692	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.14956981773037945	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.14821746856464366	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	525	0.14199666240225897	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	485	0.13117786907637258	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	483	0.13063692941007826	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	464	0.1254980025802822	Illumina PCR Primer Index 8 (95% over 22bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	417	0.1127859204223657	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2089367E-7	45.000004	6
AGGTACG	30	2.1614924E-6	45.000004	1
GGTAATG	30	2.1614924E-6	45.000004	1
GCGCGGA	30	2.1614924E-6	45.000004	3
GGCAACG	35	1.2089367E-7	45.000004	1
CAATTGG	30	2.1614924E-6	45.000004	2
ACGGGTA	30	2.1614924E-6	45.000004	5
TGCGCGG	30	2.1614924E-6	45.000004	2
TAGGGTA	75	0.0	45.000004	5
ACGTAAG	30	2.1614924E-6	45.000004	1
GCTAACG	30	2.1614924E-6	45.000004	1
TACACGG	35	1.2089367E-7	45.000004	2
CAGCGCG	20	7.027071E-4	45.0	1
AGCAACG	20	7.027071E-4	45.0	1
TGCCCGA	20	7.027071E-4	45.0	37
TGCTTAG	50	2.1827873E-11	45.0	1
CGTGCCA	20	7.027071E-4	45.0	29
TCCGACA	20	7.027071E-4	45.0	39
GCAACGG	50	2.1827873E-11	45.0	2
GACGTGC	20	7.027071E-4	45.0	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content