##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369727
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.695364417529692	31.0	31.0	34.0	30.0	34.0
2	31.796436289478454	33.0	31.0	34.0	30.0	34.0
3	31.81183143238119	33.0	31.0	34.0	30.0	34.0
4	35.61157015852237	37.0	35.0	37.0	33.0	37.0
5	32.96072237083037	37.0	35.0	37.0	28.0	37.0
6	34.049912503008976	37.0	35.0	37.0	27.0	37.0
7	35.366583993054334	37.0	35.0	37.0	32.0	37.0
8	35.283176505908415	37.0	35.0	37.0	33.0	37.0
9	37.41067868995231	39.0	37.0	39.0	34.0	39.0
10	36.938435656579045	39.0	37.0	39.0	33.0	39.0
11	36.85933404917682	39.0	37.0	39.0	33.0	39.0
12	37.165876443970824	39.0	37.0	39.0	34.0	39.0
13	37.10665707400325	39.0	37.0	39.0	33.0	39.0
14	38.112691255980764	40.0	37.0	41.0	33.0	41.0
15	38.27161392054137	40.0	38.0	41.0	34.0	41.0
16	38.2426628296013	40.0	38.0	41.0	33.0	41.0
17	38.189631809416134	40.0	37.0	41.0	33.0	41.0
18	38.059195027682584	39.0	37.0	41.0	34.0	41.0
19	37.84636502067742	39.0	37.0	41.0	34.0	41.0
20	37.66616449434312	39.0	35.0	41.0	33.0	41.0
21	37.58923746439941	39.0	35.0	40.0	33.0	41.0
22	37.544755995640024	39.0	35.0	40.0	33.0	41.0
23	37.31382073800399	39.0	35.0	40.0	33.0	41.0
24	37.04398380426639	39.0	35.0	40.0	32.0	41.0
25	36.814506379031016	39.0	35.0	40.0	32.0	41.0
26	37.085257500804644	39.0	35.0	40.0	33.0	41.0
27	36.922339996808454	39.0	35.0	40.0	32.0	41.0
28	36.975341265311975	39.0	35.0	40.0	32.0	41.0
29	37.0921544815499	39.0	35.0	40.0	33.0	41.0
30	36.68406148320247	39.0	35.0	40.0	31.0	41.0
31	36.52796793309658	39.0	35.0	40.0	31.0	41.0
32	36.33107130396211	39.0	35.0	40.0	31.0	41.0
33	36.079902198108336	39.0	35.0	40.0	30.0	41.0
34	35.967013499149374	39.0	35.0	40.0	29.0	41.0
35	35.762205627395346	39.0	35.0	40.0	27.0	41.0
36	35.481601289600164	39.0	35.0	40.0	25.0	41.0
37	35.41196071696143	39.0	35.0	40.0	25.0	41.0
38	35.38812691526451	39.0	35.0	40.0	25.0	41.0
39	35.261349590373435	39.0	35.0	40.0	24.0	41.0
40	35.12124621680321	38.0	35.0	40.0	23.0	41.0
41	35.00947455825514	38.0	35.0	40.0	23.0	41.0
42	35.064431323652315	39.0	35.0	40.0	23.0	41.0
43	34.906349820272794	38.0	35.0	40.0	22.0	41.0
44	34.80140211561503	38.0	35.0	40.0	21.0	41.0
45	34.744257249267704	38.0	34.0	40.0	22.0	41.0
46	34.530623946858086	38.0	34.0	40.0	20.0	41.0
47	34.459438991472084	38.0	34.0	40.0	20.0	41.0
48	34.42628209462658	38.0	34.0	40.0	20.0	41.0
49	34.43069345760521	38.0	34.0	40.0	20.0	41.0
50	34.322140390071596	38.0	34.0	40.0	20.0	41.0
51	33.27571424321189	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	2.0
11	6.0
12	12.0
13	14.0
14	21.0
15	44.0
16	51.0
17	102.0
18	180.0
19	308.0
20	477.0
21	852.0
22	1415.0
23	2043.0
24	3008.0
25	4471.0
26	6112.0
27	6861.0
28	7012.0
29	6896.0
30	7325.0
31	8484.0
32	10690.0
33	13987.0
34	20725.0
35	32743.0
36	40617.0
37	52693.0
38	75934.0
39	66620.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.54089098172435	23.8129755197754	23.631760731566807	18.01437276693344
2	36.414705985767874	25.310566985911226	24.29224806411217	13.982478964208727
3	26.84926986668542	24.778011884444467	34.92441720512703	13.448301043743086
4	25.59212608221742	25.46906230813546	34.220654699278114	14.718156910369002
5	18.328929182883588	33.61507274286162	31.951953738839737	16.10404433541505
6	19.572008536027933	35.131326627484604	32.50560548729198	12.79105934919549
7	64.7591330900908	9.295236755768444	20.896769778782723	5.048860375358035
8	62.44823883568146	9.429660262842583	21.15939598676862	6.962704914707338
9	56.37078168486478	12.866249962810397	23.655562076883754	7.107406275441068
10	20.10699786599302	40.673523978503056	28.49886537904995	10.720612776453978
11	19.634757537318077	26.528492644572886	36.46852948256416	17.368220335544876
12	19.83517568368012	23.81865538627149	39.58731712858407	16.75885180146432
13	19.54847766054413	25.71789455463085	40.47986757796969	14.253760206855329
14	13.990863529036288	30.44895287604097	37.83007462262697	17.730108972295774
15	12.871388889640196	29.463090334219572	41.46573011979109	16.199790656349144
16	13.65250576776919	29.851755484452042	38.28743911047881	18.208299637299955
17	13.341465459649957	29.18207217757967	36.72574629388711	20.750716068883257
18	12.080264627684752	29.269704403519352	41.30966902606518	17.340361942730716
19	14.093912535465355	28.926478185255604	38.80565931078877	18.173949968490263
20	14.148547441761083	31.366386550076136	38.98579221966478	15.499273788498
21	14.592929377621866	29.499062822028144	40.20155411966127	15.706453680688725
22	12.23470290241178	29.535035309836715	36.61701742096195	21.613244366789548
23	12.880043924300901	28.199996213422335	38.225231048854965	20.694728813421793
24	14.684889120891901	28.348484151820124	36.32572141066246	20.64090531662551
25	12.977142594400734	30.454903212370212	34.976076943258136	21.591877249970924
26	13.246530548215304	31.47268119450297	35.87133209097523	19.40945616630649
27	15.551744936128548	30.926873071212	36.09771534131941	17.423666651340042
28	11.769494789398664	31.80806378760545	36.097174401653106	20.325267021342775
29	17.530502235433172	28.24813984372253	34.30395940788744	19.917398512956858
30	16.32853429692719	34.39186210366027	32.59431959256424	16.685284006848295
31	15.871710748741638	37.23044300253971	29.678113851571563	17.219732397147084
32	19.20498097244724	34.23553054010121	28.30547944834973	18.254009039101824
33	16.345303426582316	34.38699364666362	28.63355935595723	20.63414357079683
34	16.72287931365575	33.20368812664479	29.39168629826872	20.68174626143073
35	22.010023612016433	30.47681126885513	27.04725378454914	20.4659113345793
36	18.524478872248984	36.12773749279874	27.165719571467594	18.182064063484678
37	17.79123515458703	36.49260129771426	29.033313769348734	16.682849778349972
38	18.682162784973777	35.08669910501532	26.619100038677185	19.612038071333714
39	20.760452982876554	31.741798678484397	28.43584590792666	19.06190243071239
40	21.024161070195035	32.6124410713851	29.285121184008737	17.078276674411118
41	20.332028767171455	32.66031423185215	25.593207961550007	21.414449039426387
42	20.518923421876142	30.629897194416422	27.913298190286344	20.93788119342109
43	21.563477917490474	29.424683617912677	28.96461443172936	20.047224032867494
44	19.511964233069264	30.286670976152674	27.684210241610703	22.51715454916736
45	21.339258425811476	28.86237683479973	27.714502862923183	22.08386187646561
46	21.644348397601473	31.895155073878833	28.859672136468255	17.600824392051432
47	18.541788941570402	30.825987823448113	32.006588645135466	18.625634589846022
48	17.794210322751653	29.553968198157023	30.7416012355062	21.910220243585133
49	20.40370327295545	26.743786631758027	32.93754581082799	19.914964284458534
50	19.000776248421133	25.83500799238357	31.672017461532427	23.49219829766287
51	18.739502389600975	24.52566353011817	28.4320593302626	28.302774750018255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5695.0
1	6346.5
2	6998.0
3	10977.0
4	14956.0
5	9836.0
6	4716.0
7	4623.0
8	4530.0
9	4624.0
10	4718.0
11	4969.0
12	5220.0
13	5203.0
14	5186.0
15	4998.0
16	4810.0
17	4585.5
18	4361.0
19	4352.5
20	4344.0
21	4006.0
22	3668.0
23	3788.5
24	3909.0
25	5402.0
26	7427.5
27	7960.0
28	7630.5
29	7301.0
30	7714.5
31	8128.0
32	7817.0
33	7506.0
34	8034.5
35	8563.0
36	8604.0
37	8645.0
38	9526.5
39	10408.0
40	11760.5
41	13113.0
42	15661.0
43	18209.0
44	20537.0
45	22865.0
46	28155.0
47	33445.0
48	40147.5
49	46850.0
50	44424.5
51	41999.0
52	32163.5
53	22328.0
54	16716.5
55	11105.0
56	8559.0
57	6013.0
58	5128.0
59	4243.0
60	3944.0
61	3645.0
62	3231.5
63	2818.0
64	2385.5
65	1953.0
66	1591.5
67	1230.0
68	979.0
69	728.0
70	512.0
71	296.0
72	232.0
73	168.0
74	121.0
75	65.5
76	57.0
77	45.0
78	33.0
79	25.5
80	18.0
81	12.5
82	7.0
83	6.0
84	5.0
85	4.5
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	369727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.037698474282614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03018480282066	28.118926353793665
2	11.21185540537781	8.753700613047892
3	4.778359466292778	5.596084681406144
4	2.6447765558204734	4.129839588718853
5	1.7924327552251496	3.49862247169535
6	1.2516455297678457	2.931681647265653
7	1.0089289155030265	2.757038394977448
8	0.8244168819345088	2.5746670123254085
9	0.6580759173279653	2.312079231045244
>10	3.607214249155776	22.359504577277054
>50	0.10977686901370055	2.962719911859142
>100	0.06966608995100226	4.688432149809919
>500	0.004925885148050666	1.255138365997628
>1k	0.007036978782929523	5.63040401608391
>5k	7.036978782929522E-4	2.4311609846966533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8850	2.393658023352365	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4403	1.1908786753469451	No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	2454	0.6637329705431304	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	2263	0.6120732324120229	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	2113	0.5715027574399489	RNA PCR Primer, Index 40 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2008	0.5431034249594971	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	1851	0.500639661155393	Illumina PCR Primer Index 8 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1754	0.4744040873401185	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1547	0.4184168318786564	No Hit
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1052	0.28453426447081226	RNA PCR Primer, Index 40 (95% over 24bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.2842637946376651	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.21718727601716942	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.20285237486036994	No Hit
ACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	734	0.19852485753001542	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	656	0.17742821054453692	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.14956981773037945	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.14821746856464366	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	525	0.14199666240225897	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	485	0.13117786907637258	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	483	0.13063692941007826	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	464	0.1254980025802822	Illumina PCR Primer Index 8 (95% over 22bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	417	0.1127859204223657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5579792657825909	0.0
2	0.0	0.0	0.0	2.4596526626402726	0.0
3	0.0	0.0	0.0	3.296486326397585	0.0
4	0.0	0.0	0.0	4.344286460009683	0.0
5	2.7046983314715995E-4	0.0	0.0	6.981367333194492	0.0
6	2.7046983314715995E-4	0.0	0.0	8.348592339753386	0.0
7	2.7046983314715995E-4	0.0	0.0	9.774238830272065	0.0
8	2.7046983314715995E-4	0.0	0.0	11.965855888263503	0.0
9	2.7046983314715995E-4	0.0	0.0	12.746431826726207	0.0
10	2.7046983314715995E-4	0.0	0.0	15.0754475599564	0.0
11	2.7046983314715995E-4	0.0	0.0	19.012676921079606	0.0
12	2.7046983314715995E-4	0.0	0.0	21.970805486210097	0.0
13	2.7046983314715995E-4	0.0	0.0	23.15329959672948	0.0
14	2.7046983314715995E-4	0.0	0.0	23.592001666094173	0.0
15	2.7046983314715995E-4	0.0	0.0	24.49861654680345	0.0
16	2.7046983314715995E-4	0.0	0.0	26.27776710924547	0.0
17	2.7046983314715995E-4	0.0	0.0	28.528617060696135	0.0
18	2.7046983314715995E-4	0.0	0.0	30.665058272725553	0.0
19	2.7046983314715995E-4	0.0	0.0	31.976296023822982	0.0
20	2.7046983314715995E-4	0.0	0.0	33.47713312795657	0.0
21	2.7046983314715995E-4	0.0	0.0	35.13294944648349	0.0
22	2.7046983314715995E-4	0.0	0.0	36.780651670015985	0.0
23	2.7046983314715995E-4	0.0	0.0	38.184660573882894	0.0
24	2.7046983314715995E-4	0.0	0.0	39.14969693855196	0.0
25	2.7046983314715995E-4	0.0	0.0	39.925945359684306	0.0
26	2.7046983314715995E-4	0.0	0.0	40.623216589537684	0.0
27	2.7046983314715995E-4	0.0	0.0	41.19580122631022	0.0
28	2.7046983314715995E-4	0.0	0.0	41.805169760390775	0.0
29	2.7046983314715995E-4	0.0	0.0	42.39181882848696	0.0
30	2.7046983314715995E-4	0.0	0.0	42.979279306082596	0.0
31	5.409396662943199E-4	0.0	0.0	43.48722165273296	0.0
32	5.409396662943199E-4	0.0	0.0	43.96378949873826	0.0
33	5.409396662943199E-4	0.0	0.0	44.403302977602394	0.0
34	5.409396662943199E-4	0.0	0.0	44.83416142180582	0.0
35	5.409396662943199E-4	0.0	0.0	45.24175946035859	0.0
36	5.409396662943199E-4	0.0	0.0	45.66234005090242	0.0
37	5.409396662943199E-4	0.0	0.0	46.02260586865444	0.0
38	5.409396662943199E-4	0.0	0.0	46.40369786355879	0.0
39	5.409396662943199E-4	0.0	0.0	46.779380461800194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2089367E-7	45.000004	6
AGGTACG	30	2.1614924E-6	45.000004	1
GGTAATG	30	2.1614924E-6	45.000004	1
GCGCGGA	30	2.1614924E-6	45.000004	3
GGCAACG	35	1.2089367E-7	45.000004	1
CAATTGG	30	2.1614924E-6	45.000004	2
ACGGGTA	30	2.1614924E-6	45.000004	5
TGCGCGG	30	2.1614924E-6	45.000004	2
TAGGGTA	75	0.0	45.000004	5
ACGTAAG	30	2.1614924E-6	45.000004	1
GCTAACG	30	2.1614924E-6	45.000004	1
TACACGG	35	1.2089367E-7	45.000004	2
CAGCGCG	20	7.027071E-4	45.0	1
AGCAACG	20	7.027071E-4	45.0	1
TGCCCGA	20	7.027071E-4	45.0	37
TGCTTAG	50	2.1827873E-11	45.0	1
CGTGCCA	20	7.027071E-4	45.0	29
TCCGACA	20	7.027071E-4	45.0	39
GCAACGG	50	2.1827873E-11	45.0	2
GACGTGC	20	7.027071E-4	45.0	27
>>END_MODULE