SRR3550117_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448125
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9338	2.0837935843793587	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7288	1.6263319386331938	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	4046	0.9028730822873082	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3797	0.8473082287308229	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCC	3252	0.7256903765690377	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	3062	0.6832914923291492	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2731	0.6094281729428174	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	2092	0.466834030683403	No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1463	0.32647140864714086	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1367	0.30504881450488147	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1339	0.2988005578800558	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.23453277545327755	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	1048	0.23386331938633192	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.18677824267782425	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	742	0.16557880055788005	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.16357043235704324	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	720	0.1606694560669456	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.14170153417015344	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	632	0.1410320781032078	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTC	602	0.13433751743375175	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	596	0.13299860529986052	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.1321059972105997	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	527	0.11760111576011158	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGT	498	0.11112970711297072	No Hit
AGGTACAGGAGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	490	0.1093444909344491	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.028674E-4	45.000004	13
GTACCGG	20	7.028674E-4	45.000004	2
TCGCAAC	20	7.028674E-4	45.000004	11
CGACGGT	20	7.028674E-4	45.000004	28
CAACGAG	20	7.028674E-4	45.000004	14
GGGTCAA	40	6.7993824E-9	45.000004	7
GAGTACG	20	7.028674E-4	45.000004	1
GATCCTA	20	7.028674E-4	45.000004	9
TTCACTG	40	6.7993824E-9	45.000004	19
ACTTCCG	20	7.028674E-4	45.000004	28
CGTCTCG	20	7.028674E-4	45.000004	43
CTAAGCC	20	7.028674E-4	45.000004	10
TTGAGCG	20	7.028674E-4	45.000004	1
CCGTAGG	20	7.028674E-4	45.000004	2
TAACAGT	20	7.028674E-4	45.000004	24
GTCACGT	20	7.028674E-4	45.000004	39
CGGGCGG	20	7.028674E-4	45.000004	6
GTCAAGT	20	7.028674E-4	45.000004	35
AACGAGC	20	7.028674E-4	45.000004	15
CGGTCTA	20	7.028674E-4	45.000004	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content