##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448125
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610773779637377	31.0	31.0	34.0	30.0	34.0
2	31.70377238493724	31.0	31.0	34.0	30.0	34.0
3	31.737446025104603	31.0	31.0	34.0	30.0	34.0
4	35.55929037656904	37.0	35.0	37.0	33.0	37.0
5	32.97934728033473	37.0	35.0	37.0	28.0	37.0
6	34.02631408647141	37.0	35.0	37.0	28.0	37.0
7	35.325286471408646	37.0	35.0	37.0	32.0	37.0
8	35.27403291492329	37.0	35.0	37.0	33.0	37.0
9	37.359689818688985	39.0	37.0	39.0	34.0	39.0
10	36.81916652719665	39.0	37.0	39.0	32.0	39.0
11	36.75154476987448	39.0	37.0	39.0	32.0	39.0
12	37.10056345885634	39.0	37.0	39.0	33.0	39.0
13	37.04714086471409	39.0	37.0	39.0	33.0	39.0
14	37.97062649930265	40.0	37.0	41.0	33.0	41.0
15	38.101656903765694	40.0	37.0	41.0	33.0	41.0
16	38.054770432357046	40.0	37.0	41.0	33.0	41.0
17	38.01919330543933	39.0	37.0	41.0	33.0	41.0
18	37.916246582984655	39.0	37.0	40.0	33.0	41.0
19	37.70506666666667	39.0	37.0	40.0	33.0	41.0
20	37.5460820083682	39.0	35.0	40.0	33.0	41.0
21	37.45045913528591	39.0	35.0	40.0	33.0	41.0
22	37.41643737796374	39.0	35.0	40.0	33.0	41.0
23	37.13367252440725	39.0	35.0	40.0	32.0	41.0
24	36.85465216178522	39.0	35.0	40.0	32.0	41.0
25	36.66090711297071	38.0	35.0	40.0	31.0	41.0
26	36.988846861924685	39.0	35.0	40.0	32.0	41.0
27	36.8856390516039	39.0	35.0	40.0	32.0	41.0
28	36.97650209205021	39.0	35.0	40.0	32.0	41.0
29	37.04326248256625	39.0	35.0	40.0	33.0	41.0
30	36.811378521617854	39.0	35.0	40.0	32.0	41.0
31	36.636556764295676	39.0	35.0	40.0	31.0	41.0
32	36.401530822873085	39.0	35.0	40.0	31.0	41.0
33	36.2812050209205	39.0	35.0	40.0	30.0	41.0
34	36.1869009762901	39.0	35.0	40.0	30.0	41.0
35	35.986769316596934	39.0	35.0	40.0	30.0	41.0
36	35.87792691771269	39.0	35.0	40.0	29.0	41.0
37	35.794242677824265	39.0	35.0	40.0	28.0	41.0
38	35.67195536959554	39.0	35.0	40.0	27.0	41.0
39	35.626771548117155	39.0	35.0	40.0	27.0	41.0
40	35.55058521617852	39.0	35.0	40.0	25.0	41.0
41	35.462377684797765	39.0	35.0	40.0	25.0	41.0
42	35.53930711297071	39.0	35.0	40.0	25.0	41.0
43	35.40148619246862	39.0	35.0	40.0	24.0	41.0
44	35.28358158995816	39.0	35.0	40.0	24.0	41.0
45	35.30313863319386	39.0	35.0	40.0	24.0	41.0
46	35.212840167364014	39.0	35.0	40.0	23.0	41.0
47	35.13344490934449	38.0	35.0	40.0	23.0	41.0
48	35.09112189679219	38.0	35.0	40.0	23.0	41.0
49	35.14052329149233	39.0	35.0	40.0	23.0	41.0
50	35.06855230125523	39.0	35.0	40.0	23.0	41.0
51	34.22577182705718	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	16.0
11	12.0
12	17.0
13	15.0
14	20.0
15	39.0
16	69.0
17	118.0
18	180.0
19	376.0
20	594.0
21	985.0
22	1624.0
23	2387.0
24	3464.0
25	4866.0
26	6537.0
27	7339.0
28	7205.0
29	7507.0
30	8280.0
31	10031.0
32	12635.0
33	17037.0
34	25476.0
35	40026.0
36	48767.0
37	63261.0
38	98785.0
39	80440.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.66516039051604	24.470850767085075	23.89690376569038	17.967085076708507
2	33.505829846582984	26.381032078103207	24.610097629009765	15.503040446304045
3	26.329707112970713	24.925634588563458	35.46845188284519	13.276206415620642
4	23.85294281729428	27.107615062761507	33.619414225941426	15.42002789400279
5	17.938744769874475	34.363179916317996	31.601896792189677	16.09617852161785
6	19.819693165969316	34.146276150627614	32.63375174337517	13.400278940027894
7	63.24596931659693	9.760669456066944	20.95754532775453	6.03581589958159
8	60.172496513249655	9.300529986052998	21.501589958158995	9.025383542538354
9	54.233082287308235	12.631520223152023	24.849539748953976	8.285857740585774
10	20.779246861924687	37.73366806136681	29.11018131101813	12.376903765690377
11	18.169930264993024	26.87910739191074	35.96697350069735	18.983988842398887
12	17.65712691771269	23.273863319386333	40.95241283124128	18.116596931659693
13	18.679832635983264	25.905941422594143	41.0818410041841	14.332384937238492
14	13.467670850767085	29.62588563458856	38.2355369595537	18.670906555090657
15	11.176122733612273	29.361450488145046	42.299358437935844	17.163068340306832
16	11.43252440725244	31.037099023709903	36.70136680613668	20.829009762900977
17	12.250599721059972	30.46493723849372	35.22610878661088	22.058354253835425
18	10.946276150627614	29.10058577405858	41.24987447698744	18.703263598326362
19	12.718772663877267	29.418577405857743	38.148284518828454	19.71436541143654
20	12.303040446304045	32.27581589958159	38.56357043235704	16.85757322175732
21	13.818465829846582	31.298633193863317	38.58856345885634	16.294337517433753
22	10.822873082287309	30.150739191073917	35.110069735006974	23.9163179916318
23	11.574225941422595	29.308563458856344	36.61344490934449	22.50376569037657
24	14.653277545327756	27.809874476987446	35.40284518828452	22.134002789400277
25	11.575118549511854	31.321171548117157	33.312580195258015	23.791129707112972
26	10.652161785216178	30.63497907949791	37.331324965132495	21.38153417015342
27	14.058577405857742	28.996150627615062	34.85545327754533	22.089818688981868
28	9.09076708507671	28.927196652719665	35.95358437935844	26.02845188284519
29	11.820139470013947	28.103988842398884	36.354588563458854	23.721283124128313
30	14.232189679218967	28.405244072524404	34.38750348675035	22.975062761506276
31	12.150404463040445	31.271854951185496	28.913807531380755	27.663933054393308
32	11.3497350069735	32.598940027894	30.028228730822875	26.023096234309623
33	12.048200836820083	27.637601115760113	32.86047419804742	27.453723849372384
34	11.313361227336124	25.578800557880054	33.31057182705718	29.79726638772664
35	11.18794979079498	26.151408647140865	32.39654114365411	30.264100418410038
36	12.229623430962343	25.63101813110181	35.322956764295675	26.816401673640165
37	11.480725244072525	28.044853556485354	31.693165969316595	28.781255230125524
38	11.61483960948396	31.307336122733613	29.744825662482565	27.332998605299863
39	15.791576011157602	26.01461645746165	35.54097629009763	22.652831241283124
40	15.821478382147838	23.766806136680614	34.5936959553696	25.818019525801954
41	12.999721059972105	24.682622036262202	30.153863319386332	32.163793584379356
42	14.700585774058577	23.561059972105998	34.101422594142264	27.636931659693165
43	17.33712691771269	23.307336122733613	34.42454672245467	24.930990237099024
44	14.63029288702929	24.762287308228732	32.32758716875871	28.279832635983265
45	16.57439330543933	21.88429567642957	31.482510460251046	30.058800557880055
46	17.979358437935844	22.762175732217575	33.805299860529985	25.4531659693166
47	11.838437935843793	23.67397489539749	37.75531380753138	26.73227336122734
48	12.653165969316596	23.141534170153417	35.35129707112971	28.85400278940028
49	16.827224546722455	20.20730822873082	38.88758716875872	24.077880055788007
50	16.398549511854952	19.219191073919106	35.35196652719665	29.03029288702929
51	14.86928870292887	18.467615062761507	31.47068340306834	35.192412831241285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9184.0
1	8514.5
2	7845.0
3	11956.5
4	16068.0
5	10633.0
6	5198.0
7	5203.5
8	5209.0
9	5552.5
10	5896.0
11	6020.0
12	6144.0
13	5914.0
14	5684.0
15	5106.5
16	4529.0
17	4482.5
18	4436.0
19	4490.0
20	4544.0
21	4166.0
22	3788.0
23	4030.0
24	4272.0
25	4960.5
26	6524.0
27	7399.0
28	6912.0
29	6425.0
30	6749.5
31	7074.0
32	7107.5
33	7141.0
34	7446.5
35	7752.0
36	8278.0
37	8804.0
38	9790.0
39	10776.0
40	12240.5
41	13705.0
42	17654.0
43	21603.0
44	25522.0
45	29441.0
46	39023.0
47	48605.0
48	58115.0
49	67625.0
50	63288.5
51	58952.0
52	44078.0
53	29204.0
54	21420.0
55	13636.0
56	10269.5
57	6903.0
58	5708.0
59	4513.0
60	3941.5
61	3370.0
62	3052.0
63	2734.0
64	2212.0
65	1690.0
66	1398.0
67	1106.0
68	832.0
69	558.0
70	415.5
71	273.0
72	229.5
73	186.0
74	145.0
75	73.0
76	42.0
77	33.5
78	25.0
79	19.5
80	14.0
81	14.0
82	14.0
83	9.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93056455763693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11906914149044	25.194177405097296
2	11.635666212323843	8.361521120260331
3	5.173070028147371	5.576139798225773
4	2.9286220392356266	4.209081729826959
5	1.9623596165394264	3.5254344443684755
6	1.4139556945073735	3.048253581788133
7	1.0449625053985456	2.628226493237273
8	0.878114000912107	2.5240905438989847
9	0.6824283003407022	2.2068030691215044
>10	3.9354122513378478	23.23220865715287
>50	0.13303003972362035	3.321425546845727
>100	0.07880926523911158	5.188056736724924
>500	0.006304741219128928	1.4987417417767923
>1k	0.00693521534104182	5.719502946853152
>5k	0.0012609482438257853	3.7663361848217876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9338	2.0837935843793587	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7288	1.6263319386331938	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	4046	0.9028730822873082	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3797	0.8473082287308229	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCC	3252	0.7256903765690377	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	3062	0.6832914923291492	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2731	0.6094281729428174	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	2092	0.466834030683403	No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1463	0.32647140864714086	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1367	0.30504881450488147	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1339	0.2988005578800558	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.23453277545327755	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	1048	0.23386331938633192	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.18677824267782425	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	742	0.16557880055788005	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.16357043235704324	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	720	0.1606694560669456	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.14170153417015344	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	632	0.1410320781032078	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTC	602	0.13433751743375175	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	596	0.13299860529986052	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.1321059972105997	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	527	0.11760111576011158	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGT	498	0.11112970711297072	No Hit
AGGTACAGGAGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	490	0.1093444909344491	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7270292887029288	0.0
2	0.0	0.0	0.0	3.433863319386332	0.0
3	0.0	0.0	0.0	4.4521059972106	0.0
4	0.0	0.0	0.0	5.861087866108787	0.0
5	0.0	0.0	0.0	9.3842119944212	0.0
6	0.0	0.0	0.0	11.271854951185496	0.0
7	0.0	0.0	0.0	13.342705718270572	0.0
8	0.0	0.0	0.0	16.508340306834032	0.0
9	0.0	0.0	0.0	17.526136680613668	0.0
10	0.0	0.0	0.0	20.545606694560668	0.0
11	0.0	0.0	0.0	25.152357043235703	0.0
12	0.0	0.0	0.0	29.002845188284518	0.0
13	0.0	0.0	0.0	30.369651324965133	0.0
14	0.0	0.0	0.0	30.911910739191075	0.0
15	0.0	0.0	0.0	31.981478382147838	0.0
16	0.0	0.0	0.0	34.08490934449093	0.0
17	0.0	0.0	0.0	36.597154811715484	0.0
18	0.0	0.0	0.0	39.18147838214784	0.0
19	0.0	0.0	0.0	40.753584379358436	0.0
20	0.0	0.0	0.0	42.49149232914923	0.0
21	0.0	0.0	0.0	44.39676429567643	0.0
22	0.0	0.0	0.0	46.24758716875872	0.0
23	0.0	0.0	0.0	47.83062761506276	0.0
24	0.0	0.0	0.0	48.889930264993026	0.0
25	0.0	0.0	0.0	49.6836820083682	0.0
26	0.0	0.0	0.0	50.426108786610875	0.0
27	0.0	0.0	0.0	51.055174337517435	0.0
28	0.0	0.0	0.0	51.70008368200837	0.0
29	0.0	0.0	0.0	52.319330543933056	0.0
30	0.0	0.0	0.0	52.96825662482566	0.0
31	0.0	0.0	0.0	53.51854951185495	0.0
32	0.0	0.0	0.0	53.96150627615063	0.0
33	0.0	0.0	0.0	54.39085076708508	0.0
34	0.0	0.0	0.0	54.84050209205021	0.0
35	0.0	0.0	0.0	55.24239888423989	0.0
36	0.0	0.0	0.0	55.61751743375174	0.0
37	0.0	0.0	0.0	55.950460251046024	0.0
38	0.0	0.0	0.0	56.3155369595537	0.0
39	0.0	0.0	0.0	56.654504881450485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.028674E-4	45.000004	13
GTACCGG	20	7.028674E-4	45.000004	2
TCGCAAC	20	7.028674E-4	45.000004	11
CGACGGT	20	7.028674E-4	45.000004	28
CAACGAG	20	7.028674E-4	45.000004	14
GGGTCAA	40	6.7993824E-9	45.000004	7
GAGTACG	20	7.028674E-4	45.000004	1
GATCCTA	20	7.028674E-4	45.000004	9
TTCACTG	40	6.7993824E-9	45.000004	19
ACTTCCG	20	7.028674E-4	45.000004	28
CGTCTCG	20	7.028674E-4	45.000004	43
CTAAGCC	20	7.028674E-4	45.000004	10
TTGAGCG	20	7.028674E-4	45.000004	1
CCGTAGG	20	7.028674E-4	45.000004	2
TAACAGT	20	7.028674E-4	45.000004	24
GTCACGT	20	7.028674E-4	45.000004	39
CGGGCGG	20	7.028674E-4	45.000004	6
GTCAAGT	20	7.028674E-4	45.000004	35
AACGAGC	20	7.028674E-4	45.000004	15
CGGTCTA	20	7.028674E-4	45.000004	31
>>END_MODULE