SRR3550116_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488368
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8420	1.724109687776431	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4277	0.8757740064869115	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3466	0.7097107099564263	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3265	0.6685532221603381	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	2989	0.6120384627985453	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	2892	0.5921763915735675	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1988	0.4070700783016086	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1477	0.3024358680339416	No Hit
ACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1040	0.2129541657111031	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	995	0.20373980277168038	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	849	0.17384431412377552	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	846	0.17323002326114734	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.17015856894800643	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	809	0.1656537692887331	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.15582511548668218	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	681	0.13944402581659732	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.12982013563542247	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	619	0.12674868132228156	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	574	0.11753431838285883	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	569	0.11651050027847852	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	568	0.11630573665760247	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	560	0.11466762769059397	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	534	0.10934377354781641	No Hit
AGCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	499	0.10217704681715427	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGCG	20	7.029296E-4	45.000004	1
TTGTGCG	30	2.1629276E-6	45.000004	1
CCAGGCG	20	7.029296E-4	45.000004	31
GCTATTC	20	7.029296E-4	45.000004	35
TAGCGAG	20	7.029296E-4	45.000004	1
TCGTGTA	20	7.029296E-4	45.000004	17
TACGAAG	20	7.029296E-4	45.000004	1
CGGGCTA	30	2.1629276E-6	45.000004	6
AGTCTCG	20	7.029296E-4	45.000004	1
GACGGTA	20	7.029296E-4	45.000004	9
CGAATAT	35	1.21001E-7	45.000004	14
GCGATAA	20	7.029296E-4	45.000004	9
TTTGACG	20	7.029296E-4	45.000004	1
CGGTAAC	20	7.029296E-4	45.000004	11
CATCACC	20	7.029296E-4	45.000004	43
CGTCAGG	20	7.029296E-4	45.000004	2
ACGGTAA	20	7.029296E-4	45.000004	10
CGGGTCC	25	3.8874998E-5	45.0	6
TCGTTAG	25	3.8874998E-5	45.0	1
CCGGGTA	25	3.8874998E-5	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content