##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488368
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63998460177571	31.0	31.0	34.0	30.0	34.0
2	31.740885971234807	31.0	31.0	34.0	30.0	34.0
3	31.754140320414113	31.0	31.0	34.0	30.0	34.0
4	35.56833985846738	37.0	35.0	37.0	33.0	37.0
5	33.076999311994236	37.0	35.0	37.0	28.0	37.0
6	34.06672632113488	37.0	35.0	37.0	28.0	37.0
7	35.38107328899518	37.0	35.0	37.0	32.0	37.0
8	35.36491539167186	37.0	35.0	37.0	33.0	37.0
9	37.42154277102513	39.0	37.0	39.0	34.0	39.0
10	36.75046276578318	39.0	37.0	39.0	32.0	39.0
11	36.67034899911542	39.0	37.0	39.0	32.0	39.0
12	37.05084690233594	39.0	37.0	39.0	33.0	39.0
13	37.04329112472562	39.0	37.0	39.0	33.0	39.0
14	37.992849654359006	40.0	37.0	41.0	33.0	41.0
15	38.11966386003997	40.0	37.0	41.0	33.0	41.0
16	38.08452027978901	40.0	37.0	41.0	33.0	41.0
17	38.05804229597353	40.0	37.0	41.0	33.0	41.0
18	37.97838515218032	39.0	37.0	40.0	33.0	41.0
19	37.79764849457786	39.0	37.0	40.0	33.0	41.0
20	37.657082364118864	39.0	36.0	40.0	33.0	41.0
21	37.58520214264653	39.0	35.0	40.0	33.0	41.0
22	37.558230678504735	39.0	35.0	40.0	33.0	41.0
23	37.30886135045703	39.0	35.0	40.0	33.0	41.0
24	37.039265471939196	39.0	35.0	40.0	32.0	41.0
25	36.84227058283917	39.0	35.0	40.0	32.0	41.0
26	37.168045408380564	39.0	35.0	40.0	33.0	41.0
27	37.09683681158471	39.0	35.0	40.0	33.0	41.0
28	37.20260950758445	39.0	35.0	40.0	33.0	41.0
29	37.28886003996986	39.0	35.0	40.0	33.0	41.0
30	36.93790133669692	39.0	35.0	40.0	32.0	41.0
31	36.82224470071749	39.0	35.0	40.0	32.0	41.0
32	36.56669765422796	39.0	35.0	40.0	31.0	41.0
33	36.419368181371425	39.0	35.0	40.0	30.0	41.0
34	36.367042885692754	39.0	35.0	40.0	30.0	41.0
35	36.10224052353963	39.0	35.0	40.0	30.0	41.0
36	35.92634243029846	39.0	35.0	40.0	29.0	41.0
37	35.90516168135505	39.0	35.0	40.0	29.0	41.0
38	35.741983504242704	39.0	35.0	40.0	27.0	41.0
39	35.66033605805458	39.0	35.0	40.0	26.0	41.0
40	35.534670576286736	39.0	35.0	40.0	25.0	41.0
41	35.39867272220948	39.0	35.0	40.0	24.0	41.0
42	35.55670109425679	39.0	35.0	40.0	25.0	41.0
43	35.52270419028274	39.0	35.0	40.0	25.0	41.0
44	35.432917390164796	39.0	35.0	40.0	24.0	41.0
45	35.36193198571569	39.0	35.0	40.0	23.0	41.0
46	35.20249074468433	39.0	35.0	40.0	23.0	41.0
47	35.10366772597713	39.0	35.0	40.0	23.0	41.0
48	35.03930232939095	39.0	35.0	40.0	23.0	41.0
49	35.043033122563315	39.0	35.0	40.0	23.0	41.0
50	34.94157070078302	39.0	35.0	40.0	23.0	41.0
51	33.81529297578875	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	9.0
12	18.0
13	20.0
14	27.0
15	26.0
16	44.0
17	85.0
18	176.0
19	362.0
20	606.0
21	1036.0
22	1590.0
23	2463.0
24	3713.0
25	5393.0
26	7169.0
27	8225.0
28	7806.0
29	8029.0
30	8663.0
31	10455.0
32	13339.0
33	18270.0
34	27147.0
35	39748.0
36	52504.0
37	73047.0
38	112672.0
39	85699.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.941748845133176	23.823632998067033	24.09351145038168	16.14110670641811
2	36.45488647904859	22.979597352815908	26.1059283163516	14.459587851783901
3	27.720284703338464	23.214256462339875	35.41263964878944	13.652819185532222
4	24.998771418274742	25.54774268584346	34.16870065196737	15.284785243914426
5	18.25406251023818	33.509157029125575	32.60860662451267	15.62817383612358
6	21.04867640795466	33.299274317727615	32.80006061003178	12.85198866428595
7	67.73150574976248	7.443976673328309	19.497182452576745	5.32733512433247
8	65.58496871211872	6.857124135897521	19.57437833764702	7.983528814336729
9	59.8835304524457	9.800396422370016	22.67286144874357	7.643211676440717
10	24.764726599613407	32.26050846902336	29.65161517544147	13.323149755921765
11	19.698874619139666	25.41505585951577	35.22609999017134	19.659969531173214
12	18.660723061298036	22.057342004390133	40.87655210824624	18.40538282606559
13	19.4547963830554	24.036996691019887	41.7150591357337	14.793147790191002
14	15.696974412737935	27.451430069128197	38.33318317334469	18.518412344789176
15	13.450307964485797	27.753046882678632	41.357132326442354	17.439512826393212
16	13.75212954165711	27.975010647708288	37.41481833371556	20.858041476919045
17	13.601833043934084	27.877338400550407	35.91779150149068	22.60303705402483
18	13.647085804147691	26.510745994823576	41.29078891327851	18.551379287750223
19	14.422935163647086	27.7499754283655	38.27380172329063	19.553287684696784
20	14.617460603479342	29.617010123513417	38.64135242276316	17.124176850244076
21	14.655956164204042	28.457433738492284	39.50422632113488	17.38238377616879
22	12.242407364937916	27.207761360285687	35.59528879861089	24.954542476165514
23	12.761073616616978	26.564189299872226	37.128149264489075	23.54658781902172
24	15.714584084133277	25.227492382793304	36.427038626609445	22.630884906463976
25	12.558767159191431	29.112472561674803	34.504717753824984	23.824042525308784
26	12.539314615208202	28.228508010352847	38.87703535039151	20.35514202404744
27	17.355764505454903	26.394849785407725	36.71964584084133	19.52973986829604
28	12.207597549388986	25.88826458736035	36.6934360973692	25.210701765881467
29	16.568653146807325	23.37110539593094	37.87389837171969	22.186343085542052
30	20.124168659699244	25.8202830652295	34.528060806604856	19.527487468466404
31	17.819758870360054	28.77338400550404	30.61400419355896	22.79285293057694
32	18.062198997477314	27.222913868230513	33.372579694001246	21.342307440290927
33	18.407430462274352	24.90806113422665	34.507993971759	22.17651443174
34	17.66004324607673	22.658527995282245	35.46116862693706	24.22026013170396
35	19.211332437833764	22.40052583297841	36.312370998918844	22.075770730268978
36	20.64058251154867	23.33936703469515	36.508534547718114	19.511515906038067
37	21.46537037643744	24.090030468826786	33.58410051436621	20.860498640369556
38	19.51499688759296	24.86219408315041	33.2095878517839	22.413221177472725
39	23.241080496674638	20.773883628738986	36.3623333224126	19.62270255217377
40	23.57955476198277	20.37029453199227	37.75329259902369	18.296858107001277
41	20.010320086492154	22.6059037447171	35.70156111784556	21.68221505094519
42	20.592053533401042	20.984994921862203	38.474060544507424	19.948891000229334
43	23.201356354224682	19.982676997673885	35.246985879500706	21.568980768600728
44	21.92342659633719	20.042467974969693	31.988787144120828	26.04531828457229
45	24.163335845100416	18.057898961438916	32.61925433279822	25.159510860662454
46	24.623030173967173	19.94131474625692	36.304589981325556	19.131065098450346
47	19.64399796874488	20.59573927857681	40.05033089801134	19.709931854666973
48	19.018854634210268	19.440053402352326	38.52054188644629	23.02055007699112
49	22.265586606821085	17.2728352390001	39.62339874848475	20.838179405694067
50	21.276373554368835	16.32007011106379	37.19387838679029	25.209677947777088
51	20.14464502178685	16.39378501457917	33.57242898797628	29.889140975657703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4579.0
1	5390.5
2	6202.0
3	10468.0
4	14734.0
5	9879.0
6	5024.0
7	4918.0
8	4812.0
9	5095.5
10	5379.0
11	5575.0
12	5771.0
13	5611.5
14	5452.0
15	5017.5
16	4583.0
17	4396.0
18	4209.0
19	4080.0
20	3951.0
21	4027.5
22	4104.0
23	4253.0
24	4402.0
25	4938.0
26	6054.0
27	6634.0
28	6591.5
29	6549.0
30	6930.5
31	7312.0
32	7441.0
33	7570.0
34	8430.5
35	9291.0
36	9975.0
37	10659.0
38	11251.0
39	11843.0
40	13848.0
41	15853.0
42	20121.0
43	24389.0
44	28815.0
45	33241.0
46	42715.0
47	52189.0
48	63579.5
49	74970.0
50	71111.0
51	67252.0
52	51849.5
53	36447.0
54	27172.0
55	17897.0
56	13459.0
57	9021.0
58	7608.5
59	6196.0
60	5538.0
61	4880.0
62	4064.5
63	3249.0
64	2636.5
65	2024.0
66	1490.0
67	956.0
68	754.0
69	552.0
70	404.0
71	256.0
72	236.5
73	217.0
74	172.0
75	87.0
76	47.0
77	37.0
78	27.0
79	28.5
80	30.0
81	19.5
82	9.0
83	6.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	488368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.118240764161264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27417823283922	30.44061239842626
2	11.73838557940244	9.888003000316612
3	4.88376845386539	6.170872067289542
4	2.825932575762857	4.760932344370656
5	1.793214011780751	3.7763509744924586
6	1.2541626884310004	3.1693875641258784
7	0.9256746172828617	2.7291450479994683
8	0.7368494962981331	2.482784359362858
9	0.598096577547613	2.267169808803409
>10	2.8014784841310565	18.929976501151376
>50	0.10362008609489616	2.973124512985152
>100	0.05329032999154215	4.172682369048924
>500	0.006908005739644353	2.042906050789467
>1k	0.003947431851225345	4.44617823505492
>5k	4.934289814031681E-4	1.7498747657830431
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8420	1.724109687776431	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4277	0.8757740064869115	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3466	0.7097107099564263	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3265	0.6685532221603381	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	2989	0.6120384627985453	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	2892	0.5921763915735675	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1988	0.4070700783016086	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1477	0.3024358680339416	No Hit
ACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1040	0.2129541657111031	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	995	0.20373980277168038	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	849	0.17384431412377552	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	846	0.17323002326114734	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.17015856894800643	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	809	0.1656537692887331	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.15582511548668218	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	681	0.13944402581659732	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.12982013563542247	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	619	0.12674868132228156	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	574	0.11753431838285883	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	569	0.11651050027847852	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	568	0.11630573665760247	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	560	0.11466762769059397	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	534	0.10934377354781641	No Hit
AGCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	499	0.10217704681715427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6605674409461717	0.0
2	0.0	0.0	0.0	3.017806244471382	0.0
3	0.0	0.0	0.0	3.964428463781411	0.0
4	0.0	0.0	0.0	5.206934115257347	0.0
5	0.0	0.0	0.0	8.443427906824361	0.0
6	0.0	0.0	0.0	10.123718179733316	0.0
7	0.0	0.0	0.0	11.815475215411329	0.0
8	0.0	0.0	0.0	14.493578612849326	0.0
9	0.0	0.0	0.0	15.384300363660191	0.0
10	0.0	0.0	0.0	18.353168102742195	0.0
11	0.0	0.0	0.0	22.980825934541166	0.0
12	0.0	0.0	0.0	26.23881990630017	0.0
13	0.0	0.0	0.0	27.460439668446746	0.0
14	0.0	0.0	0.0	27.93528650525833	0.0
15	0.0	0.0	0.0	28.96340464567703	0.0
16	0.0	0.0	0.0	31.185294695803165	0.0
17	0.0	0.0	0.0	34.079833240507156	0.0
18	0.0	0.0	0.0	36.99505291091963	0.0
19	0.0	0.0	0.0	38.768101104085446	0.0
20	0.0	0.0	0.0	40.58517347573961	0.0
21	0.0	0.0	0.0	42.658609900730596	0.0
22	0.0	0.0	0.0	44.90404776725748	0.0
23	0.0	0.0	0.0	46.83660682108574	0.0
24	0.0	0.0	0.0	48.115560397077616	0.0
25	0.0	0.0	0.0	49.07037316122268	0.0
26	0.0	0.0	0.0	49.90662778888051	0.0
27	0.0	0.0	0.0	50.62903384333126	0.0
28	0.0	0.0	0.0	51.34673033450185	0.0
29	0.0	0.0	0.0	52.043336172722206	0.0
30	0.0	0.0	0.0	52.73687055662943	0.0
31	0.0	0.0	0.0	53.321265930609705	0.0
32	0.0	0.0	0.0	53.85897519903024	0.0
33	0.0	0.0	0.0	54.305359892540054	0.0
34	0.0	0.0	0.0	54.71570618877568	0.0
35	0.0	0.0	0.0	55.14550502899453	0.0
36	0.0	0.0	0.0	55.55134652557088	0.0
37	0.0	0.0	0.0	55.933844969367364	0.0
38	0.0	0.0	0.0	56.29811945090587	0.0
39	0.0	0.0	0.0	56.67529404055958	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGCG	20	7.029296E-4	45.000004	1
TTGTGCG	30	2.1629276E-6	45.000004	1
CCAGGCG	20	7.029296E-4	45.000004	31
GCTATTC	20	7.029296E-4	45.000004	35
TAGCGAG	20	7.029296E-4	45.000004	1
TCGTGTA	20	7.029296E-4	45.000004	17
TACGAAG	20	7.029296E-4	45.000004	1
CGGGCTA	30	2.1629276E-6	45.000004	6
AGTCTCG	20	7.029296E-4	45.000004	1
GACGGTA	20	7.029296E-4	45.000004	9
CGAATAT	35	1.21001E-7	45.000004	14
GCGATAA	20	7.029296E-4	45.000004	9
TTTGACG	20	7.029296E-4	45.000004	1
CGGTAAC	20	7.029296E-4	45.000004	11
CATCACC	20	7.029296E-4	45.000004	43
CGTCAGG	20	7.029296E-4	45.000004	2
ACGGTAA	20	7.029296E-4	45.000004	10
CGGGTCC	25	3.8874998E-5	45.0	6
TCGTTAG	25	3.8874998E-5	45.0	1
CCGGGTA	25	3.8874998E-5	45.0	5
>>END_MODULE