FastQCFastQC Report
Sat 18 Jun 2016
SRR3550115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454072
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95882.1115594002713225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47481.045649148152716No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC47351.042786166070579TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC44250.9745150548811643TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC29380.6470339505629064No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT28570.6291953698972851Illumina PCR Primer Index 5 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21510.4737134198981659No Hit
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC19640.4325305237935834TruSeq Adapter, Index 23 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19260.4241618069381068No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.28806004334114416No Hit
ACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC11850.26097182825631177Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG10000.22022939093359642No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.1911591113303617No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG8240.18146901812928345Illumina PCR Primer Index 5 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG7200.15856516147218944Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG7140.15724378512658788TruSeq Adapter, Index 23 (95% over 23bp)
AGTTATGGGAGATACATTTTCTTAACATTTTTTTCCCTTTTTAATGTTATG7110.15658309695378705No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC6720.1479941507073768No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.14601208618897443No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.138083828115365No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6020.13257809334202506No Hit
AGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG5690.12531052344121638Illumina PCR Primer Index 5 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG4780.1052696488662591TruSeq Adapter, Index 23 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT4600.10130551982945436Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGT4570.10064483165665357No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.028772E-445.0000041
ATGGGCG207.028772E-445.0000045
CGGGTCC207.028772E-445.0000046
CGGGTAC207.028772E-445.0000046
GATACTA302.1625892E-645.0000049
GGTACTA207.028772E-445.0000041
ACAGTGC253.8870647E-545.00000416
ACAGTAC207.028772E-445.00000410
GGAGCGA207.028772E-445.0000048
CATGCGG253.8870647E-545.0000042
TATGGCA253.8870647E-545.00000414
GACGTAG207.028772E-445.0000041
TACGGGC207.028772E-445.0000044
TAACGCC302.1625892E-645.00000412
ACGGGTA253.8870647E-545.0000045
ACGGGCA207.028772E-445.0000045
CCGTCCG207.028772E-445.0000041
TACATGG406.7993824E-945.0000042
TATTGCG253.8870647E-545.0000041
TGCATGA207.028772E-445.00000412
Produced by FastQC (version 0.11.5)