##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454072
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.596101499321694	31.0	31.0	34.0	30.0	34.0
2	31.685003699853766	31.0	31.0	34.0	30.0	34.0
3	31.725065628358497	31.0	31.0	34.0	30.0	34.0
4	35.54302181151888	37.0	35.0	37.0	33.0	37.0
5	33.126645113550275	37.0	35.0	37.0	28.0	37.0
6	34.088005866910976	37.0	35.0	37.0	28.0	37.0
7	35.3703751827904	37.0	35.0	37.0	32.0	37.0
8	35.35208513187336	37.0	35.0	37.0	33.0	37.0
9	37.39514218009479	39.0	37.0	39.0	34.0	39.0
10	36.81903530717595	39.0	37.0	39.0	32.0	39.0
11	36.749907503655805	39.0	37.0	39.0	32.0	39.0
12	37.07880027836995	39.0	37.0	39.0	33.0	39.0
13	37.016508395144385	39.0	37.0	39.0	33.0	39.0
14	37.92751149597421	40.0	37.0	41.0	33.0	41.0
15	38.067214010112934	40.0	37.0	41.0	33.0	41.0
16	38.04195369897285	40.0	37.0	41.0	33.0	41.0
17	38.030440106414844	39.0	37.0	41.0	33.0	41.0
18	37.96007461371765	39.0	37.0	40.0	33.0	41.0
19	37.78138048591413	39.0	37.0	40.0	33.0	41.0
20	37.636291160873164	39.0	35.0	40.0	33.0	41.0
21	37.555858982716394	39.0	35.0	40.0	33.0	41.0
22	37.51939780475343	39.0	35.0	40.0	33.0	41.0
23	37.2571948942018	39.0	35.0	40.0	33.0	41.0
24	36.9796662203351	39.0	35.0	40.0	32.0	41.0
25	36.81048600221991	39.0	35.0	40.0	32.0	41.0
26	37.14092698955232	39.0	35.0	40.0	33.0	41.0
27	37.07867254532321	39.0	35.0	40.0	33.0	41.0
28	37.20906596310717	39.0	35.0	40.0	33.0	41.0
29	37.321116474920274	39.0	35.0	40.0	33.0	41.0
30	37.045517010518154	39.0	35.0	40.0	33.0	41.0
31	36.921488222132176	39.0	35.0	40.0	32.0	41.0
32	36.80603736852305	39.0	35.0	40.0	32.0	41.0
33	36.73322072270477	39.0	35.0	40.0	31.0	41.0
34	36.687573776845966	39.0	35.0	41.0	31.0	41.0
35	36.532593949858175	39.0	35.0	41.0	31.0	41.0
36	36.396500995436845	39.0	35.0	41.0	30.0	41.0
37	36.337975034796244	39.0	35.0	40.0	30.0	41.0
38	36.28027713666555	39.0	35.0	40.0	30.0	41.0
39	36.20835682446837	39.0	35.0	40.0	30.0	41.0
40	36.09533510104124	39.0	35.0	40.0	30.0	41.0
41	35.970143501471135	39.0	35.0	40.0	29.0	41.0
42	36.08557233214116	39.0	35.0	40.0	30.0	41.0
43	36.006904191405766	39.0	35.0	40.0	29.0	41.0
44	35.906354058387215	39.0	35.0	40.0	29.0	41.0
45	35.88569433922373	39.0	35.0	40.0	28.0	41.0
46	35.754197572191195	39.0	35.0	40.0	27.0	41.0
47	35.67769648866259	39.0	35.0	40.0	27.0	41.0
48	35.65789346182984	39.0	35.0	40.0	26.0	41.0
49	35.6851050053736	39.0	35.0	40.0	27.0	41.0
50	35.585440194506596	39.0	35.0	40.0	26.0	41.0
51	34.6050670378266	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	9.0
11	8.0
12	11.0
13	11.0
14	14.0
15	32.0
16	41.0
17	85.0
18	151.0
19	337.0
20	544.0
21	893.0
22	1283.0
23	1985.0
24	2905.0
25	4332.0
26	5578.0
27	6265.0
28	6338.0
29	6563.0
30	7522.0
31	9369.0
32	11863.0
33	16779.0
34	24968.0
35	38338.0
36	49562.0
37	65386.0
38	103729.0
39	89151.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.595799785056116	22.83734738103208	22.72635176800155	16.840501065910253
2	33.72174456914322	25.47018974964323	25.87497137017918	14.933094311034372
3	26.524427844042354	24.353846967000827	36.30943991261298	12.812285276343841
4	24.649835268415583	26.29054423087088	34.156697616237075	14.902922884476471
5	18.451258831198576	33.695096812840255	32.00924082524357	15.844403530717596
6	21.12748638982364	33.85079899223031	31.82953364224176	13.192180975704293
7	66.6218132807132	8.48786095597174	19.260601842879545	5.629723920435525
8	64.0352190841981	7.84787434591871	19.379305484592752	8.73760108529044
9	58.50746137176483	10.742129001568033	22.707632269772198	8.042777356894943
10	22.61910005461689	37.06218397082401	27.927949752462165	12.390766222096936
11	18.104177311087227	26.043667083634315	35.58048062862277	20.271674976655685
12	18.508298243450376	22.775683151570675	40.80498246974048	17.911036135238465
13	19.163921140259692	25.141387268979372	41.48835426980743	14.206337320953505
14	13.807061435190896	28.739935516834336	37.142347469123834	20.31065557885093
15	11.746595253616167	28.7102045490583	41.29235011187653	18.250850085449002
16	11.86375728959284	29.624156521432727	36.479897461195584	22.032188727778852
17	12.0535950245776	29.653006571645022	35.04223999718106	23.25115840659631
18	11.658283267851795	27.988072376187034	41.88520763226977	18.468436723691397
19	12.71252136225092	28.92382705826389	37.77308444475766	20.590567134727532
20	12.667814795891399	31.61767296816364	38.85705350693282	16.85745872901214
21	14.041605736535177	30.393417783963777	38.40558325551895	17.1593932239821
22	10.879552141510597	29.426390528374352	34.67842104335877	25.015636286756287
23	10.886599482020474	28.58379287866242	36.794385031448755	23.735222607868355
24	15.082850296869218	27.355573565425743	34.4341866488134	23.127389488891627
25	11.369122077555982	31.099473211296885	33.112369844429956	24.419034866717173
26	11.294023855247627	29.504131503373916	38.099244172730316	21.102600468648145
27	16.561470427597385	28.31335118659596	35.11359431984355	20.011584065963106
28	10.160723409503339	27.087554396659563	36.97673496714177	25.774987226695327
29	14.458499973572472	24.092214450571717	38.43157032364911	23.0177152522067
30	21.014508712274704	27.429350411388505	32.47436529889533	19.081775577441462
31	14.483606124138904	29.688903962367203	32.55078489754929	23.276705015944607
32	14.892131644320724	28.33977871350799	35.600081044415866	21.16800859775542
33	19.185283391180253	24.783074049930406	30.69975686675241	25.33188569213693
34	13.674263112457934	22.58606564597685	38.17676491833894	25.56290632322627
35	15.910031889215807	22.657860427421202	37.45419228668581	23.97791539667718
36	20.85021758663824	23.25732482954245	32.703624094857204	23.188833488962104
37	16.821120879508094	24.241750207015627	36.89062527528674	22.046503638189538
38	16.307766169241884	25.95403372152434	33.72042319279762	24.01777691643616
39	20.988741873535474	21.39550555858983	35.89474796948501	21.721004598389683
40	20.854622174456914	21.339787522683626	39.65956940749485	18.14602089536461
41	16.841822442255854	22.840650821896087	34.83896826934935	25.478558466498708
42	17.88747159040857	21.068464913053436	36.07291354675029	24.971149949787698
43	21.2884740745961	20.855282862629714	32.863510632674995	24.992732430099192
44	19.398465441603975	20.925095579555666	32.73489666836977	26.941542310470584
45	21.35101922162124	18.205042372134816	34.496952025229476	25.946986381014465
46	22.99899575397734	20.53044451100266	36.76773727514579	19.702822459874206
47	15.058404834475589	21.44527740094082	41.980346729153084	21.515971035430503
48	16.170783488081184	19.876583449320812	37.74753783540936	26.20509522718864
49	20.0069592487535	17.33095191951937	39.358075371306754	23.304013460420375
50	19.68542433799045	16.223418312514315	37.31456685283391	26.776590496661324
51	18.219137053154565	16.54627459962297	32.41270106943392	32.82188727778855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5840.0
1	6599.0
2	7358.0
3	11451.5
4	15545.0
5	9870.0
6	4195.0
7	4175.5
8	4156.0
9	4544.5
10	4933.0
11	5242.5
12	5552.0
13	5385.0
14	5218.0
15	5108.0
16	4998.0
17	4457.0
18	3916.0
19	3704.0
20	3492.0
21	3580.5
22	3669.0
23	3911.5
24	4154.0
25	5732.0
26	6842.5
27	6375.0
28	6227.5
29	6080.0
30	6339.0
31	6598.0
32	6483.0
33	6368.0
34	7198.5
35	8029.0
36	8522.0
37	9015.0
38	9922.0
39	10829.0
40	12433.5
41	14038.0
42	17950.5
43	21863.0
44	26496.0
45	31129.0
46	40673.5
47	50218.0
48	61726.5
49	73235.0
50	69598.5
51	65962.0
52	48349.5
53	30737.0
54	22289.5
55	13842.0
56	10187.0
57	6532.0
58	5210.0
59	3888.0
60	3507.0
61	3126.0
62	2674.5
63	2223.0
64	1897.0
65	1571.0
66	1237.5
67	904.0
68	708.0
69	512.0
70	406.0
71	300.0
72	231.5
73	163.0
74	136.0
75	83.0
76	57.0
77	38.0
78	19.0
79	14.0
80	9.0
81	6.0
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	454072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.33240317294988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00690409219634	25.071866611947623
2	12.16740991910854	8.841424855032018
3	5.567738118597061	6.068679182588096
4	3.1625285199871565	4.596090449365034
5	2.0973140768505707	3.8100230310219065
6	1.463309402071642	3.189932829770107
7	1.1120926430969025	2.828349879226769
8	0.8600176528927859	2.4997206480603813
9	0.6726285319675298	2.1994389908166334
>10	3.6458405562233183	21.390655838132545
>50	0.1515614881683492	3.7380180729071233
>100	0.07915559503529167	5.55630848876151
>500	0.006749701902234173	1.7768237634084674
>1k	0.0061360926383947035	6.295128307072131
>5k	6.136092638394703E-4	2.1375390518896342
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9588	2.1115594002713225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4748	1.045649148152716	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	4735	1.042786166070579	TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	4425	0.9745150548811643	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC	2938	0.6470339505629064	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	2857	0.6291953698972851	Illumina PCR Primer Index 5 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2151	0.4737134198981659	No Hit
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1964	0.4325305237935834	TruSeq Adapter, Index 23 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1926	0.4241618069381068	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.28806004334114416	No Hit
ACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1185	0.26097182825631177	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	1000	0.22022939093359642	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.1911591113303617	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	824	0.18146901812928345	Illumina PCR Primer Index 5 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	720	0.15856516147218944	Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	714	0.15724378512658788	TruSeq Adapter, Index 23 (95% over 23bp)
AGTTATGGGAGATACATTTTCTTAACATTTTTTTCCCTTTTTAATGTTATG	711	0.15658309695378705	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	672	0.1479941507073768	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	663	0.14601208618897443	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.138083828115365	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	602	0.13257809334202506	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	569	0.12531052344121638	Illumina PCR Primer Index 5 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	478	0.1052696488662591	TruSeq Adapter, Index 23 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	460	0.10130551982945436	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGT	457	0.10064483165665357	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4045878186719286E-4	0.0	0.0	0.6611286315826564	0.0
2	4.4045878186719286E-4	0.0	0.0	3.543931358903434	0.0
3	4.4045878186719286E-4	0.0	0.0	4.700576120086683	0.0
4	4.4045878186719286E-4	0.0	0.0	6.056968938846703	0.0
5	4.4045878186719286E-4	0.0	0.0	9.617197272679222	0.0
6	4.4045878186719286E-4	0.0	0.0	11.29248224951109	0.0
7	4.4045878186719286E-4	0.0	0.0	13.17169964234747	0.0
8	4.4045878186719286E-4	0.0	0.0	16.252929050899418	0.0
9	4.4045878186719286E-4	0.0	0.0	17.30210186930707	0.0
10	4.4045878186719286E-4	0.0	0.0	20.841848869782766	0.0
11	4.4045878186719286E-4	0.0	0.0	25.794587642488416	0.0
12	4.4045878186719286E-4	0.0	0.0	29.611383216758576	0.0
13	4.4045878186719286E-4	0.0	0.0	31.038689899399213	0.0
14	4.4045878186719286E-4	0.0	0.0	31.578031677795593	0.0
15	4.4045878186719286E-4	0.0	0.0	32.731372998114836	0.0
16	4.4045878186719286E-4	0.0	0.0	35.011407882450364	0.0
17	4.4045878186719286E-4	0.0	0.0	37.960059197660286	0.0
18	4.4045878186719286E-4	0.0	0.0	40.92368611145369	0.0
19	4.4045878186719286E-4	0.0	0.0	42.6674624288659	0.0
20	4.4045878186719286E-4	0.0	0.0	44.524216423827056	0.0
21	6.606881728007893E-4	0.0	0.0	46.493507637555275	0.0
22	6.606881728007893E-4	0.0	0.0	48.637660987684775	0.0
23	6.606881728007893E-4	0.0	0.0	50.36425941260417	0.0
24	6.606881728007893E-4	0.0	0.0	51.551295829736254	0.0
25	6.606881728007893E-4	0.0	0.0	52.49740129318698	0.0
26	6.606881728007893E-4	0.0	0.0	53.27128737292764	0.0
27	6.606881728007893E-4	0.0	0.0	53.91920224105428	0.0
28	6.606881728007893E-4	0.0	0.0	54.565575503444386	0.0
29	6.606881728007893E-4	0.0	0.0	55.17759298084885	0.0
30	6.606881728007893E-4	0.0	0.0	55.8327754188763	0.0
31	8.809175637343857E-4	0.0	0.0	56.338201871068904	0.0
32	8.809175637343857E-4	0.0	0.0	56.80354657411159	0.0
33	8.809175637343857E-4	0.0	0.0	57.21031025916595	0.0
34	8.809175637343857E-4	0.0	0.0	57.59042618791734	0.0
35	8.809175637343857E-4	0.0	0.0	57.959530647122044	0.0
36	8.809175637343857E-4	0.0	0.0	58.321807995207806	0.0
37	8.809175637343857E-4	0.0	0.0	58.6514913934354	0.0
38	8.809175637343857E-4	0.0	0.0	58.99769199598302	0.0
39	8.809175637343857E-4	0.0	0.0	59.340368928275694	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.028772E-4	45.000004	1
ATGGGCG	20	7.028772E-4	45.000004	5
CGGGTCC	20	7.028772E-4	45.000004	6
CGGGTAC	20	7.028772E-4	45.000004	6
GATACTA	30	2.1625892E-6	45.000004	9
GGTACTA	20	7.028772E-4	45.000004	1
ACAGTGC	25	3.8870647E-5	45.000004	16
ACAGTAC	20	7.028772E-4	45.000004	10
GGAGCGA	20	7.028772E-4	45.000004	8
CATGCGG	25	3.8870647E-5	45.000004	2
TATGGCA	25	3.8870647E-5	45.000004	14
GACGTAG	20	7.028772E-4	45.000004	1
TACGGGC	20	7.028772E-4	45.000004	4
TAACGCC	30	2.1625892E-6	45.000004	12
ACGGGTA	25	3.8870647E-5	45.000004	5
ACGGGCA	20	7.028772E-4	45.000004	5
CCGTCCG	20	7.028772E-4	45.000004	1
TACATGG	40	6.7993824E-9	45.000004	2
TATTGCG	25	3.8870647E-5	45.000004	1
TGCATGA	20	7.028772E-4	45.000004	12
>>END_MODULE