FastQCFastQC Report
Sat 18 Jun 2016
SRR3550114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751812
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286523.811059147765665No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC55930.743935983995999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37480.49852888754103425No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC27410.3645858273078908TruSeq Adapter, Index 15 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA24930.33159885716109877No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC24070.32015982719084024TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG19720.26229961745755587No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT19400.2580432342128085TruSeq Adapter, Index 21 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17260.22957867126356057No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.203508323889483No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.17797002442099888No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC12110.1610775034184078No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11740.15615606029166867No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.14578112613259697No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGT10900.14498305427420685No Hit
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC9750.12968667698839603TruSeq Adapter, Index 15 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.11824764701813752No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC8820.11731656318334904No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG8700.11572041946656876TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA351.2111741E-745.024
CACGATC207.031729E-445.029
TACGGTT207.031729E-445.033
CCGCTCG207.031729E-445.019
ACGATCC207.031729E-445.030
CGCTAGA253.8895138E-545.024
AGCGCCG207.031729E-445.03
GGACGTA207.031729E-445.08
TCCGTGC207.031729E-445.034
GCGATCG253.8895138E-545.09
ATGCGCA207.031729E-445.022
CGGTCTA351.2111741E-745.031
TCGACGA207.031729E-445.011
TACGTAG253.8895138E-545.01
ACGATAG302.1644973E-644.9999961
CGATGCA302.1644973E-644.99999610
GTAACGG650.044.9999962
CGTTTTT109050.043.8445661
CGTTATT7950.043.3018881
CGCGAGG650.041.538462
Produced by FastQC (version 0.11.5)