##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550114_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 751812 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.826028315589536 33.0 31.0 34.0 30.0 34.0 2 31.943581640090873 33.0 31.0 34.0 30.0 34.0 3 31.91394657174932 33.0 31.0 34.0 30.0 34.0 4 35.67767207759387 37.0 35.0 37.0 33.0 37.0 5 33.15097258357142 37.0 35.0 37.0 28.0 37.0 6 34.188509893430805 37.0 35.0 37.0 28.0 37.0 7 35.50340776683532 37.0 35.0 37.0 32.0 37.0 8 35.502795911743895 37.0 35.0 37.0 33.0 37.0 9 37.62304006852777 39.0 37.0 39.0 35.0 39.0 10 37.0187719802291 39.0 37.0 39.0 33.0 39.0 11 36.912217416056144 39.0 37.0 39.0 33.0 39.0 12 37.261736976797394 39.0 37.0 39.0 34.0 39.0 13 37.25396907737573 39.0 37.0 39.0 34.0 39.0 14 38.34905268870409 40.0 38.0 41.0 34.0 41.0 15 38.4693620213564 40.0 38.0 41.0 34.0 41.0 16 38.42423371800397 40.0 38.0 41.0 34.0 41.0 17 38.343781424079424 40.0 38.0 41.0 34.0 41.0 18 38.1541901432805 39.0 37.0 41.0 34.0 41.0 19 37.84304852808947 39.0 37.0 41.0 34.0 41.0 20 37.57945869446085 39.0 35.0 41.0 34.0 41.0 21 37.48421546876081 39.0 35.0 40.0 33.0 41.0 22 37.42984017280916 39.0 35.0 40.0 33.0 41.0 23 37.19225817092571 39.0 35.0 40.0 33.0 41.0 24 36.89636770894851 39.0 35.0 40.0 32.0 41.0 25 36.70863859581917 38.0 35.0 40.0 32.0 41.0 26 36.97078923986316 39.0 35.0 40.0 33.0 41.0 27 36.870918527504216 39.0 35.0 40.0 33.0 41.0 28 36.89780024793433 39.0 35.0 40.0 33.0 41.0 29 36.91585529361063 39.0 35.0 40.0 33.0 41.0 30 36.56089687315446 39.0 35.0 40.0 31.0 41.0 31 36.315650721190934 39.0 35.0 40.0 31.0 41.0 32 35.84063303059808 38.0 35.0 40.0 29.0 41.0 33 35.4580945768357 39.0 35.0 40.0 25.0 41.0 34 35.17841428442217 39.0 35.0 40.0 22.0 41.0 35 34.76611706118019 38.0 35.0 40.0 20.0 41.0 36 34.53515240512256 38.0 35.0 40.0 18.0 41.0 37 34.50501854186951 38.0 35.0 40.0 18.0 41.0 38 34.38914781886961 38.0 34.0 40.0 18.0 41.0 39 34.3681532085149 38.0 34.0 40.0 18.0 41.0 40 34.25265092868962 38.0 34.0 40.0 17.0 41.0 41 33.98719493703213 38.0 33.0 40.0 15.0 41.0 42 33.982358621570285 38.0 33.0 40.0 15.0 41.0 43 33.94791916064123 38.0 33.0 40.0 15.0 41.0 44 33.934379871563635 38.0 34.0 40.0 15.0 41.0 45 33.918339957329756 38.0 33.0 40.0 15.0 41.0 46 33.76611440094066 38.0 33.0 40.0 15.0 41.0 47 33.61828090001224 38.0 33.0 40.0 14.0 41.0 48 33.49764435789798 38.0 33.0 40.0 13.0 41.0 49 33.47272722435928 38.0 33.0 40.0 12.0 41.0 50 33.383613190531676 37.0 33.0 40.0 12.0 41.0 51 32.41930163391912 36.0 31.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 15.0 10 9.0 11 14.0 12 19.0 13 30.0 14 47.0 15 82.0 16 116.0 17 214.0 18 444.0 19 785.0 20 1307.0 21 2197.0 22 3527.0 23 5412.0 24 8571.0 25 13385.0 26 18357.0 27 19183.0 28 17267.0 29 15974.0 30 15422.0 31 17040.0 32 20385.0 33 26194.0 34 38325.0 35 58331.0 36 78594.0 37 101082.0 38 149582.0 39 139849.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.29570690544977 20.442743664639565 22.610439844003555 24.651109585907115 2 40.79197990987108 22.14170563917575 24.33480710603183 12.731507344921337 3 25.922438056322587 21.727240320718476 40.161503141742884 12.18881848121605 4 21.975041632748614 24.174660686448206 39.89401605720579 13.956281623597388 5 18.147355987933153 31.729341909945575 36.670204785238866 13.453097316882412 6 19.52536006342011 32.613738541018236 36.892068761871315 10.968832633690337 7 65.06626656664166 6.834421371300271 24.270030273525826 3.829281788532239 8 64.93631386570047 6.305432741164014 23.91488829654222 4.843365096593297 9 59.81841204982097 9.217729964405995 25.94172479290035 5.022133192872686 10 22.73773230541678 35.5918766925774 31.33762163945242 10.332769362553403 11 18.956733864317144 24.506658579538502 40.50467404085064 16.031933515293716 12 18.628460306566 21.896431554697184 43.32399589259017 16.151112246146642 13 18.776103600368177 22.747043143764664 44.317595356285885 14.159257899581279 14 15.159640974073305 25.22638638383 43.31508409017148 16.298888551925213 15 13.713933802599588 26.380132267109328 44.22581709257101 15.680116837720067 16 14.929397242927752 26.931865945209704 42.032449601762146 16.106287210100398 17 14.76978287124973 26.5204599022096 40.593925077013935 18.115832149526742 18 14.170031869669545 25.944518044404717 44.2673168292073 15.618133256718437 19 14.646214745175657 27.13125089783084 42.4885476688321 15.733986688161403 20 14.68212797880321 28.882220555138783 41.76975094837539 14.665900517682612 21 15.604033987220209 26.90898788526919 42.745260783280926 14.741717344229674 22 13.870355886844052 26.335972290945076 40.43417769335951 19.359494128851363 23 13.285236202667688 26.290748218969632 41.53870914537145 18.88530643299123 24 14.169100785834758 25.988944044521766 41.36433044431321 18.47762472533027 25 13.303458843434262 28.513777380515343 39.060430001117304 19.122333774933097 26 13.73468367091773 29.47372481418227 40.00521406947482 16.786377445425185 27 15.180789878320644 27.706527695753724 40.39946156751954 16.713220858406093 28 12.228988098088351 27.47096348555224 40.685836352705195 19.614212063654215 29 15.640213244800563 26.361111554484367 39.494714104057934 18.503961096657143 30 17.246066835857903 28.84111985443169 37.33287045165547 16.579942858054938 31 16.28226205487542 30.160332636350578 34.78622315153256 18.771182157241437 32 15.919538395237106 31.17814027975079 34.600937468409654 18.30138385660245 33 17.29222199166813 30.33896772065357 33.66839050188079 18.70041978579751 34 17.55026522588094 28.18803105031577 33.82627571786564 20.435428005937656 35 18.49624640202604 29.908008917122896 32.57915542715466 19.016589253696402 36 18.690709911520432 31.90092735949945 31.92699770687353 17.48136502210659 37 16.942001457811262 31.929524934425096 32.42632466627295 18.70214894149069 38 16.209903539714716 32.58780120562055 31.92433746734556 19.27795778731917 39 21.785765590333753 28.889935249769888 31.31141828010194 18.012880879794416 40 21.988475842364846 26.73926460338489 34.3548653120727 16.917394242177565 41 19.006081307560933 29.170191484041226 33.11399658425245 18.709730624145397 42 17.912456837613657 30.866360207073047 33.3116523811804 17.909530574132894 43 18.259751107989764 29.52613153288322 33.260841806196225 18.953275552930787 44 18.44197751565551 28.810394087883672 30.824461434507562 21.923166961953257 45 21.142652684447707 26.559033375365114 31.473426867355137 20.824887072832038 46 21.13201172633584 27.656515192627946 32.717620894585345 18.49385218645087 47 17.731161513782702 28.812123243576853 34.921762355482485 18.53495288715796 48 17.12808521279256 28.644661165291325 34.25071693455279 19.976536687363332 49 19.644538794273036 26.266141003335942 34.58976446239219 19.49955573999883 50 18.655727761727668 24.898644873984455 34.21373960511404 22.231887759173834 51 18.47682665347188 23.949205386452995 32.207652977074055 25.36631498300107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5271.0 1 7251.5 2 9232.0 3 27113.0 4 44994.0 5 28840.0 6 12686.0 7 12152.5 8 11619.0 9 12140.0 10 12661.0 11 12887.5 12 13114.0 13 13058.5 14 13003.0 15 12226.5 16 11450.0 17 10635.5 18 9821.0 19 9420.5 20 9020.0 21 9586.5 22 10153.0 23 10323.0 24 10493.0 25 12355.0 26 14164.0 27 14111.0 28 14122.5 29 14134.0 30 14529.5 31 14925.0 32 16106.0 33 17287.0 34 18324.5 35 19362.0 36 20417.5 37 21473.0 38 22486.0 39 23499.0 40 26344.5 41 29190.0 42 33676.0 43 38162.0 44 41476.5 45 44791.0 46 51588.5 47 58386.0 48 66543.0 49 74700.0 50 73039.5 51 71379.0 52 57546.0 53 43713.0 54 34331.0 55 24949.0 56 20375.0 57 15801.0 58 13979.0 59 12157.0 60 10825.5 61 9494.0 62 8124.5 63 6755.0 64 5501.0 65 4247.0 66 3325.0 67 2403.0 68 1829.0 69 1255.0 70 1038.0 71 821.0 72 642.0 73 463.0 74 375.0 75 225.0 76 163.0 77 114.0 78 65.0 79 55.5 80 46.0 81 35.0 82 24.0 83 15.0 84 6.0 85 11.5 86 17.0 87 13.5 88 10.0 89 6.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 751812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.85900820496814 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30881559146718 30.36184505953222 2 10.535078877520574 8.609057485931995 3 4.271071828905126 5.235352767037285 4 2.312364591986979 3.7792369534749524 5 1.5419071191899725 3.1500397817140944 6 1.0948181015207819 2.683990907579117 7 0.8495212077084628 2.429741579723817 8 0.7104113720589265 2.322136326388668 9 0.5800220611043374 2.132921353835219 >10 3.6398823418462145 24.484381307074155 >50 0.10395913145517623 2.8559717330419563 >100 0.0447461555252947 3.1517958577178478 >500 0.002355060817120626 0.7328255285019718 >1k 0.004373684374652592 3.363216916212576 >5k 3.364372595886609E-4 0.7688413395069459 >10k+ 3.364372595886609E-4 3.938645102727161 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28652 3.811059147765665 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC 5593 0.743935983995999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3748 0.49852888754103425 No Hit CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC 2741 0.3645858273078908 TruSeq Adapter, Index 15 (95% over 21bp) GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 2493 0.33159885716109877 No Hit GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC 2407 0.32015982719084024 TruSeq Adapter, Index 15 (95% over 21bp) CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG 1972 0.26229961745755587 No Hit CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT 1940 0.2580432342128085 TruSeq Adapter, Index 21 (95% over 23bp) GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1726 0.22957867126356057 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1530 0.203508323889483 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1338 0.17797002442099888 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC 1211 0.1610775034184078 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1174 0.15615606029166867 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.14578112613259697 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGT 1090 0.14498305427420685 No Hit TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC 975 0.12968667698839603 TruSeq Adapter, Index 15 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 889 0.11824764701813752 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC 882 0.11731656318334904 No Hit CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG 870 0.11572041946656876 TruSeq Adapter, Index 21 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.27653189893217983 0.0 2 0.0 0.0 0.0 1.2463222188525855 0.0 3 0.0 0.0 0.0 1.7021542619697478 0.0 4 0.0 0.0 0.0 2.279825275467803 0.0 5 0.0 0.0 0.0 3.890600309651881 0.0 6 0.0 0.0 0.0 4.8590605098083035 0.0 7 0.0 0.0 0.0 5.9122493389304775 0.0 8 0.0 0.0 0.0 7.711901379600219 0.0 9 0.0 0.0 0.0 8.41447063893633 0.0 10 0.0 0.0 0.0 10.354051278777142 0.0 11 0.0 0.0 0.0 13.576266407027289 0.0 12 0.0 0.0 0.0 15.690890807808335 0.0 13 0.0 0.0 0.0 16.505855187201057 0.0 14 0.0 0.0 0.0 16.83612392459817 0.0 15 0.0 0.0 0.0 17.45303347113374 0.0 16 0.0 0.0 0.0 18.75482168414444 0.0 17 0.0 0.0 0.0 20.50193399413683 0.0 18 0.0 0.0 0.0 22.169505142243008 0.0 19 0.0 0.0 0.0 23.215777348592468 0.0 20 0.0 0.0 0.0 24.337201321606997 0.0 21 0.0 0.0 0.0 25.64258085798045 0.0 22 0.0 0.0 0.0 27.000899160960454 0.0 23 0.0 0.0 0.0 28.24376306842668 0.0 24 0.0 0.0 0.0 29.063515878969742 0.0 25 0.0 0.0 0.0 29.73615744361622 0.0 26 0.0 0.0 0.0 30.334179289503226 0.0 27 1.330119763983549E-4 0.0 0.0 30.873542853798558 0.0 28 1.330119763983549E-4 0.0 0.0 31.411044250424307 0.0 29 1.330119763983549E-4 0.0 0.0 31.953334078200403 0.0 30 1.330119763983549E-4 0.0 0.0 32.51663979824743 0.0 31 1.330119763983549E-4 0.0 0.0 32.977792320420534 0.0 32 1.330119763983549E-4 0.0 0.0 33.43189520784451 0.0 33 1.330119763983549E-4 0.0 0.0 33.85580437662607 0.0 34 1.330119763983549E-4 0.0 0.0 34.25470729384474 0.0 35 1.330119763983549E-4 0.0 0.0 34.71147042079669 0.0 36 1.330119763983549E-4 0.0 0.0 35.11569381707129 0.0 37 1.330119763983549E-4 0.0 0.0 35.52324251275585 0.0 38 1.330119763983549E-4 0.0 0.0 35.913100615579424 0.0 39 1.330119763983549E-4 0.0 0.0 36.288194389022784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCACGA 35 1.2111741E-7 45.0 24 CACGATC 20 7.031729E-4 45.0 29 TACGGTT 20 7.031729E-4 45.0 33 CCGCTCG 20 7.031729E-4 45.0 19 ACGATCC 20 7.031729E-4 45.0 30 CGCTAGA 25 3.8895138E-5 45.0 24 AGCGCCG 20 7.031729E-4 45.0 3 GGACGTA 20 7.031729E-4 45.0 8 TCCGTGC 20 7.031729E-4 45.0 34 GCGATCG 25 3.8895138E-5 45.0 9 ATGCGCA 20 7.031729E-4 45.0 22 CGGTCTA 35 1.2111741E-7 45.0 31 TCGACGA 20 7.031729E-4 45.0 11 TACGTAG 25 3.8895138E-5 45.0 1 ACGATAG 30 2.1644973E-6 44.999996 1 CGATGCA 30 2.1644973E-6 44.999996 10 GTAACGG 65 0.0 44.999996 2 CGTTTTT 10905 0.0 43.844566 1 CGTTATT 795 0.0 43.301888 1 CGCGAGG 65 0.0 41.53846 2 >>END_MODULE