##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271325
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92771399917409	33.0	31.0	34.0	30.0	34.0
2	32.25584645940259	34.0	31.0	34.0	30.0	34.0
3	32.312025642538295	34.0	31.0	34.0	30.0	34.0
4	35.842034098283285	37.0	35.0	37.0	35.0	37.0
5	30.378327335653747	37.0	35.0	37.0	0.0	37.0
6	32.8284820954516	37.0	35.0	37.0	17.0	37.0
7	34.98010894145872	37.0	35.0	37.0	32.0	37.0
8	35.499464731677584	37.0	35.0	37.0	33.0	37.0
9	37.29718600672527	39.0	37.0	39.0	34.0	39.0
10	37.30438636855249	39.0	37.0	39.0	34.0	39.0
11	37.18520834562366	39.0	37.0	39.0	34.0	39.0
12	37.021196782884	39.0	37.0	39.0	33.0	39.0
13	36.934698051245746	39.0	37.0	39.0	33.0	39.0
14	38.08848012899927	40.0	37.0	41.0	33.0	41.0
15	38.19368139539457	40.0	37.0	41.0	33.0	41.0
16	38.26593435982144	40.0	37.0	41.0	34.0	41.0
17	38.240879397479006	40.0	37.0	41.0	34.0	41.0
18	38.19152144416259	40.0	37.0	41.0	34.0	41.0
19	38.19860539201227	40.0	37.0	41.0	34.0	41.0
20	38.12100682358956	40.0	37.0	41.0	34.0	41.0
21	38.089868444339565	40.0	37.0	41.0	34.0	41.0
22	38.079613788763695	40.0	36.0	41.0	34.0	41.0
23	37.99873911077026	40.0	36.0	41.0	34.0	41.0
24	37.97784909444871	40.0	36.0	41.0	34.0	41.0
25	37.91290110711266	40.0	36.0	41.0	34.0	41.0
26	37.846266690264095	40.0	36.0	41.0	33.0	41.0
27	37.77269384303778	40.0	36.0	41.0	33.0	41.0
28	37.72342398678544	40.0	36.0	41.0	33.0	41.0
29	37.719925274811715	40.0	36.0	41.0	33.0	41.0
30	37.57998072876723	40.0	36.0	41.0	33.0	41.0
31	33.043484553517	38.0	33.0	41.0	9.0	41.0
32	33.976371895463394	38.0	33.0	41.0	16.0	41.0
33	35.88112481072896	38.0	34.0	41.0	29.0	41.0
34	36.66382003028337	38.0	35.0	41.0	31.0	41.0
35	36.86546319784477	39.0	35.0	40.0	31.0	41.0
36	37.03670973197255	39.0	35.0	41.0	32.0	41.0
37	37.157156509940414	39.0	35.0	41.0	32.0	41.0
38	37.154938351719665	39.0	35.0	41.0	32.0	41.0
39	37.145932786659586	39.0	35.0	41.0	32.0	41.0
40	37.10235148368828	39.0	35.0	41.0	32.0	41.0
41	37.018027648319666	39.0	35.0	41.0	32.0	41.0
42	36.99884529919572	39.0	35.0	41.0	32.0	41.0
43	36.98893437948597	39.0	35.0	41.0	32.0	41.0
44	36.91806186459009	39.0	35.0	41.0	32.0	41.0
45	36.88668200499479	39.0	35.0	41.0	32.0	41.0
46	36.821421745029795	39.0	35.0	41.0	32.0	41.0
47	36.72342791969009	39.0	35.0	41.0	31.0	41.0
48	36.75067193675889	39.0	35.0	41.0	32.0	41.0
49	36.72426326863705	39.0	35.0	41.0	32.0	41.0
50	36.54997345289363	39.0	35.0	41.0	31.0	41.0
51	35.9717428666942	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	8.0
11	11.0
12	16.0
13	35.0
14	27.0
15	37.0
16	91.0
17	161.0
18	299.0
19	481.0
20	837.0
21	1416.0
22	2226.0
23	3249.0
24	4818.0
25	6109.0
26	7662.0
27	9498.0
28	12076.0
29	15116.0
30	20514.0
31	28790.0
32	43400.0
33	50524.0
34	89816.0
35	124833.0
36	147917.0
37	147844.0
38	222077.0
39	331153.0
40	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03315438617191	21.668338151141526	24.207106758696636	12.091400703989933
2	32.11995359172517	24.71744046565591	23.973413564588125	19.189192378030796
3	31.95941242404578	24.2243328810493	25.100741352525908	18.715513342379015
4	29.52258470493383	25.167915363892	25.71765677541148	19.59184315576269
5	22.67437515977425	38.99750260554933	20.005584724598354	18.322537510078067
6	26.68963482980355	33.20755904273101	23.66507384028474	16.437732287180697
7	75.3122136353804	7.2020136471791245	10.448547774959197	7.03722494248127
8	75.0302243722101	7.223408648457317	10.341258136196489	7.405108843136097
9	68.12962853715612	9.017914380665841	12.980945076986607	9.871512005191434
10	39.631958783159305	25.603130592098793	19.600259571706687	15.16465105303522
11	30.732346174267004	24.87994808565866	24.488860047588147	19.898845692486187
12	29.646313885119856	21.293846970680196	28.70461919650758	20.35521994769237
13	24.93658191257153	24.011877372033116	29.767840638703714	21.28370007669164
14	18.668711777082965	28.207028100603697	28.977012172339883	24.14724794997345
15	18.306176626747682	28.16006921912178	33.19458045739681	20.339173696733724
16	20.83479834031424	27.435785499380565	29.891176528425067	21.838239631880125
17	19.834975321023343	27.96578372957348	27.243781094527364	24.95545985487582
18	19.936916209466503	28.567360824336813	28.946335516095413	22.54938745010127
19	22.17418834680353	29.374471515938094	26.50274320099109	21.948596936267283
20	24.79476137101056	28.426012231333452	27.47393467445382	19.30529172320217
21	23.43484160226535	28.23329990364384	28.049475940455824	20.282382553634985
22	21.153127642420312	26.31427841031994	27.893575600259574	24.639018347000178
23	21.5468900556506	26.912237232808288	28.730655025268913	22.810217686272196
24	23.669478693489076	24.73616109177433	28.05686980119167	23.537490413544923
25	20.861620750004917	27.604743083003953	25.80677639470631	25.726859772284822
26	19.346980512457478	28.037205277958037	26.37421587713606	26.24159833244843
27	21.26576603150257	28.025878512575463	26.285174915934164	24.423180539987808
28	18.12542032918412	29.273081234145476	26.989872770534678	25.611625666135723
29	19.522860008259098	25.765402237822745	25.845161544058364	28.86657620985979
30	20.764556663323695	27.150020647749397	24.97001160206871	27.1154110868582
31	22.90602324346646	29.863174247340375	22.823353587792266	24.407448921400903
32	20.616836764792637	28.2614595009144	24.552809077143927	26.568894657149038
33	20.392110593278666	26.653688081332465	25.195524354512024	27.758676970876838
34	19.10565748333432	27.85110023007492	27.43413367942894	25.60910860716182
35	18.87912217568285	24.864373783257626	27.806815723752777	28.449688317306748
36	18.91223723280829	27.963817277250115	27.395827188169825	25.728118301771772
37	19.267614496686527	29.10333706959275	28.187284919277133	23.441763514443593
38	19.14522250408039	27.623621025308243	26.546713074941497	26.68444339566987
39	21.08988653570094	24.845653157139207	28.207264074882506	25.857196232277346
40	20.702416769905412	25.081234145478142	29.420565158397732	24.79578392621871
41	17.871905295656106	26.962106463728787	28.692741824474467	26.47324641614064
42	19.46803531748373	24.622500147483926	30.14642203999764	25.76304249503471
43	21.12905826598234	24.36151259512713	28.996991327945253	25.512437810945272
44	19.983245826204943	24.61864590093013	26.756572866890842	28.641535405974082
45	20.028238255363497	23.66208483275323	26.14083731539929	30.16883959648398
46	21.246809431105344	25.716162271645725	26.61711206811791	26.419916229131026
47	17.573083200597804	25.015947928342474	31.36019507207048	26.050773798989248
48	18.65305881658899	22.813757300454252	30.408117515190845	28.125066367765918
49	19.34548600869172	22.875031954850257	31.546772068511196	26.23270996794683
50	18.80541954260319	22.21092167620396	30.67870135488565	28.304957426307197
51	17.393664090614124	22.225237449118048	28.582069887715573	31.799028572552256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1439.0
1	2014.0
2	2589.0
3	2859.5
4	3130.0
5	2708.0
6	2286.0
7	2242.5
8	2199.0
9	2374.0
10	2549.0
11	2639.0
12	2729.0
13	2970.0
14	3211.0
15	3523.5
16	3836.0
17	3777.0
18	3718.0
19	3935.5
20	4153.0
21	4476.5
22	4800.0
23	5236.0
24	5672.0
25	6518.5
26	10308.0
27	13251.0
28	13957.5
29	14664.0
30	17080.0
31	19496.0
32	22209.0
33	24922.0
34	28431.5
35	31941.0
36	31916.5
37	31892.0
38	36369.0
39	40846.0
40	46332.0
41	51818.0
42	57917.5
43	64017.0
44	72014.0
45	80011.0
46	98121.0
47	116231.0
48	129713.0
49	143195.0
50	138161.5
51	133128.0
52	113143.5
53	93159.0
54	84330.5
55	75502.0
56	69394.5
57	63287.0
58	59585.0
59	55883.0
60	53931.0
61	51979.0
62	46771.0
63	41563.0
64	35550.0
65	29537.0
66	23133.0
67	16729.0
68	13753.0
69	10777.0
70	8992.0
71	7207.0
72	6120.5
73	5034.0
74	3706.5
75	1900.0
76	1421.0
77	1221.5
78	1022.0
79	657.5
80	293.0
81	333.5
82	374.0
83	230.0
84	86.0
85	44.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1271325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.985105072328047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.96072222320761	16.730206804658142
2	13.724106169079498	6.857964693164736
3	6.076854674856068	4.5549255768164025
4	3.141723835290939	3.139852005319263
5	1.871164109054018	2.337561593614187
6	1.186316438434897	1.7784144517995533
7	0.7568102290051951	1.3236288164055219
8	0.5428809831689793	1.085115072499656
9	0.3837608258882131	0.8629474101714354
>10	3.5451163893942934	21.457978477163444
>50	1.593836723821374	27.402428545807027
>100	0.20347608897124206	7.867492120278492
>500	0.007867265302927558	1.3749683293275528
>1k	0.004648838588093556	2.1645319868925395
>5k	7.15205936629778E-4	1.0619841160820906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	6093	0.47926376025013273	Illumina PCR Primer Index 11 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	5793	0.4556663323697717	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	4725	0.37165948911568636	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	2419	0.19027392680864452	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2257	0.17753131575324957	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	2124	0.16706978939295616	No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1970	0.1549564430810375	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	1631	0.12829134957622954	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	1609	0.12656087153166973	No Hit
ACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1552	0.1220773602344011	RNA PCR Primer, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCTT	1455	0.11444752521975106	RNA PCR Primer, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.865809293453681E-5	0.0	0.0	0.5073446994277624	0.0
2	1.5731618586907361E-4	0.0	0.0	1.6834405049849566	0.0
3	2.359742788036104E-4	0.0	0.0	2.37059760486107	0.0
4	2.359742788036104E-4	0.0	0.0	3.2143629677698464	0.0
5	2.359742788036104E-4	0.0	0.0	5.305331052248638	0.0
6	2.359742788036104E-4	0.0	0.0	6.568737340963168	0.0
7	2.359742788036104E-4	0.0	0.0	7.679468271291762	0.0
8	2.359742788036104E-4	0.0	0.0	9.411204845338524	0.0
9	2.359742788036104E-4	0.0	0.0	10.05053782471044	0.0
10	2.359742788036104E-4	0.0	0.0	11.43963974593436	0.0
11	2.359742788036104E-4	0.0	0.0	13.29148722789216	0.0
12	2.359742788036104E-4	0.0	0.0	14.961319882799442	0.0
13	3.1463237173814723E-4	0.0	0.0	15.603563211609934	0.0
14	3.1463237173814723E-4	0.0	0.0	15.909621851218217	0.0
15	3.1463237173814723E-4	0.0	0.0	16.4748589070458	0.0
16	3.1463237173814723E-4	0.0	0.0	17.393192142056517	0.0
17	3.1463237173814723E-4	0.0	0.0	18.438794171435312	0.0
18	3.1463237173814723E-4	0.0	0.0	19.450415904666393	0.0
19	3.9329046467268404E-4	0.0	0.0	20.293316028552887	0.0
20	3.9329046467268404E-4	0.0	0.0	20.95789825575679	0.0
21	5.506066505417576E-4	0.0	0.0	21.73913043478261	0.0
22	6.292647434762945E-4	0.0	0.0	22.56826539240556	0.0
23	6.292647434762945E-4	0.0	0.0	23.29467288065601	0.0
24	6.292647434762945E-4	0.0	0.0	23.90277859713291	0.0
25	6.292647434762945E-4	0.0	0.0	24.437889603366568	0.0
26	6.292647434762945E-4	0.0	0.0	24.93831239061609	0.0
27	6.292647434762945E-4	0.0	0.0	25.4248126954162	0.0
28	6.292647434762945E-4	0.0	0.0	25.95347373802922	0.0
29	6.292647434762945E-4	0.0	0.0	26.502113936247614	0.0
30	6.292647434762945E-4	0.0	0.0	27.054136432462194	0.0
31	6.292647434762945E-4	0.0	0.0	27.577527382848604	0.0
32	7.865809293453681E-4	0.0	0.0	28.172497197805438	0.0
33	7.865809293453681E-4	0.0	0.0	28.741824474465616	0.0
34	7.865809293453681E-4	0.0	0.0	29.32413033645999	0.0
35	7.865809293453681E-4	0.0	0.0	29.879377814484886	0.0
36	7.865809293453681E-4	0.0	0.0	30.45712150708906	0.0
37	7.865809293453681E-4	0.0	0.0	31.04052858238452	0.0
38	7.865809293453681E-4	0.0	0.0	31.59554008613061	0.0
39	7.865809293453681E-4	0.0	0.0	32.207814681533044	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	45.000004	1
TAGCGAG	60	0.0	45.000004	1
AATTGCG	55	1.8189894E-12	45.000004	1
ACTCGAG	30	2.1656851E-6	45.000004	1
CGTAACA	30	2.1656851E-6	45.000004	15
GCGTAAA	30	2.1656851E-6	45.000004	41
TCTTGCG	35	1.2120654E-7	45.0	1
CGAAAAC	20	7.033569E-4	45.0	6
GACCGGT	20	7.033569E-4	45.0	19
CGTTCGA	45	3.8562575E-10	45.0	19
AATGCGT	20	7.033569E-4	45.0	28
CTAACGG	90	0.0	45.0	2
TACTACC	20	7.033569E-4	45.0	6
TTAACGG	80	0.0	42.1875	2
TATCTCG	235	0.0	42.12766	14
TTACGGG	310	0.0	42.09677	3
ATCTCGA	225	0.0	42.0	15
TATTGCG	60	3.6379788E-12	41.250004	1
CGTAAGG	175	0.0	41.142857	2
CGCATCG	55	6.184564E-11	40.909092	21
>>END_MODULE