FastQCFastQC Report
Sat 18 Jun 2016
SRR3550110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738095
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC59560.3426740195443862No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT58750.3380137449333897No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC40420.23255345651417214No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC22550.1297397437999649No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG21480.12358357857309296No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC19890.11443563211447015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG600.045.0000041
CCGTTGC302.1661454E-645.00000441
ACGCGAT207.034285E-445.035
CTATCGA207.034285E-445.012
CCGGCAT253.891634E-545.016
CGCATCG551.8189894E-1245.021
ATTCGCG253.891634E-545.01
CCGCTTA207.034285E-445.024
CGCTATA253.891634E-545.020
GATATCG207.034285E-445.013
TATCGAT207.034285E-445.013
CTAACGG950.042.631582
TATAGCG950.042.631581
GTACGAG700.041.7857171
TCTACGG1150.041.086962
TAACACG501.0822987E-940.51
CGGTAAG451.9292202E-840.031
CGCATGG2200.039.8863642
ATACGCA1250.039.627
GATCGCG403.4595905E-739.3751
Produced by FastQC (version 0.11.5)