##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1738095
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05085107545905	33.0	31.0	34.0	30.0	34.0
2	32.35417569235283	34.0	31.0	34.0	30.0	34.0
3	32.414340412923345	34.0	31.0	34.0	30.0	34.0
4	35.910377741147634	37.0	35.0	37.0	35.0	37.0
5	30.42106789329697	37.0	35.0	37.0	0.0	37.0
6	32.899421493071436	37.0	35.0	37.0	17.0	37.0
7	35.082281463326225	37.0	35.0	37.0	32.0	37.0
8	35.647619951728764	37.0	35.0	37.0	35.0	37.0
9	37.49283267025105	39.0	37.0	39.0	35.0	39.0
10	37.40589495971164	39.0	37.0	39.0	35.0	39.0
11	37.29607415014714	39.0	37.0	39.0	34.0	39.0
12	37.17273624284058	39.0	37.0	39.0	34.0	39.0
13	37.146473006366165	39.0	37.0	39.0	34.0	39.0
14	38.35818065180557	40.0	38.0	41.0	34.0	41.0
15	38.452246281129625	40.0	38.0	41.0	34.0	41.0
16	38.49815804084357	40.0	38.0	41.0	34.0	41.0
17	38.47797559972268	40.0	38.0	41.0	34.0	41.0
18	38.42452685267491	40.0	38.0	41.0	34.0	41.0
19	38.446548663910775	40.0	37.0	41.0	34.0	41.0
20	38.38196531259799	40.0	37.0	41.0	34.0	41.0
21	38.340288074012065	40.0	37.0	41.0	34.0	41.0
22	38.32392763341475	40.0	37.0	41.0	34.0	41.0
23	38.2627606661316	40.0	37.0	41.0	34.0	41.0
24	38.25231992497533	40.0	37.0	41.0	34.0	41.0
25	38.182918079851795	40.0	37.0	41.0	34.0	41.0
26	38.10881050805624	40.0	37.0	41.0	34.0	41.0
27	38.059455898555605	40.0	37.0	41.0	34.0	41.0
28	38.03026531921443	40.0	37.0	41.0	34.0	41.0
29	38.03213403179918	40.0	37.0	41.0	34.0	41.0
30	37.8757449966774	40.0	36.0	41.0	33.0	41.0
31	33.34597821177784	39.0	34.0	41.0	9.0	41.0
32	34.28561097063164	39.0	34.0	41.0	16.0	41.0
33	36.21758419418961	39.0	34.0	41.0	30.0	41.0
34	37.06241430991977	39.0	35.0	41.0	31.0	41.0
35	37.231952798897645	39.0	35.0	41.0	32.0	41.0
36	37.392198355095665	39.0	35.0	41.0	33.0	41.0
37	37.53231152497418	40.0	36.0	41.0	33.0	41.0
38	37.517839933950675	40.0	36.0	41.0	33.0	41.0
39	37.51232470031845	40.0	36.0	41.0	33.0	41.0
40	37.460493241163455	40.0	35.0	41.0	33.0	41.0
41	37.403960658076805	40.0	35.0	41.0	33.0	41.0
42	37.35503410342933	40.0	35.0	41.0	33.0	41.0
43	37.36286969354379	39.0	35.0	41.0	33.0	41.0
44	37.30388154847692	39.0	35.0	41.0	33.0	41.0
45	37.25487904861357	39.0	35.0	41.0	33.0	41.0
46	37.19926183551532	39.0	35.0	41.0	33.0	41.0
47	37.12904127795086	39.0	35.0	41.0	33.0	41.0
48	37.12595744191198	39.0	35.0	41.0	33.0	41.0
49	37.102386808546136	39.0	35.0	41.0	33.0	41.0
50	36.91438327594292	39.0	35.0	41.0	32.0	41.0
51	36.313118673029955	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	14.0
11	17.0
12	16.0
13	27.0
14	40.0
15	52.0
16	125.0
17	156.0
18	271.0
19	466.0
20	872.0
21	1325.0
22	2100.0
23	3481.0
24	4936.0
25	6819.0
26	8662.0
27	10786.0
28	13861.0
29	18453.0
30	24325.0
31	33898.0
32	52689.0
33	62470.0
34	111699.0
35	162569.0
36	200689.0
37	203192.0
38	302446.0
39	511111.0
40	520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06312658398994	21.331285113874674	23.62730460648008	10.978283695655302
2	31.998596164191255	23.02935109991111	25.424329510182126	19.54772322571551
3	32.63072501790754	23.984534792402027	25.30408291836752	18.080657271322913
4	28.12124768784215	25.743702156671528	26.03413507316919	20.100915082317133
5	22.28226880579025	39.33277525106511	20.63281926476976	17.75213667837489
6	27.584740765032983	32.490629108305356	22.89955382185669	17.025076304804973
7	79.93763286816889	6.4565515693906255	7.977009311919084	5.6288062505214045
8	80.58759734076676	5.134241799211205	8.192647697622972	6.085513162399064
9	74.15279371956078	8.217560029802744	10.473248010033974	7.156398240602499
10	38.63459707323248	30.75930832319292	17.19773660242967	13.408358001144933
11	27.651940774238465	25.806759699556125	26.880463956227942	19.660835569977476
12	27.537275005106167	22.58432364168817	29.366691694067352	20.51170965913831
13	23.970438900060124	24.410691015163152	30.5651302144014	21.053739870375324
14	19.055114939056843	27.961187391943476	29.574505421165124	23.409192247834554
15	18.802136822210525	27.93230519620619	32.680089408231424	20.585468573351857
16	21.971238626197074	27.583072271653737	30.33907812864084	20.106610973508353
17	21.42840293539766	26.221178934408073	28.478420339509636	23.87199779068463
18	21.386805669425435	26.878047517540754	29.35368895256013	22.38145786047368
19	22.712049686582148	28.537508018836714	27.394129780017778	21.356312514563356
20	24.976597941999717	28.037995621643237	27.880351764431747	19.1050546719253
21	24.21639783786272	27.82615449673349	28.12751892157793	19.829928743825857
22	21.776197503588698	25.8138364128543	28.274001133424814	24.135964950132184
23	21.172663174337423	27.195463999378628	29.15019029454661	22.48168253173733
24	23.327953880541628	25.29263360173063	28.531524456373212	22.847888061354528
25	21.69213995782739	27.669028447812117	26.188844683403385	24.44998691095711
26	20.037627402414714	28.82713545577198	26.879543408156632	24.255693733656674
27	21.262761816816685	28.478477873764092	27.57611062686447	22.68264968255475
28	18.54375048544527	28.372614845563675	27.73421475811161	25.34941991087944
29	20.98337547717472	26.834321484153627	27.70964763145858	24.47265540721307
30	22.456597596794193	27.00226397291287	27.23050236034279	23.310636069950146
31	24.408274576475968	28.351269637160225	23.482203216740167	23.75825256962364
32	22.638866114913167	28.33423949784103	24.69013488905957	24.336759498186233
33	22.317594838026693	27.5222010304385	27.279176339613198	22.881027791921614
34	20.164202762219556	27.053929733415032	28.15421481564587	24.627652688719547
35	20.166791803670108	26.701129685086254	27.3640393649369	25.76803914630673
36	19.735169826735593	28.93518478564175	27.76660654337076	23.563038844251896
37	20.72257270172229	29.160316323331003	27.415129782894493	22.701981192052216
38	21.633109812754768	27.06716261194008	26.117674810640384	25.182052764664764
39	22.44491814313947	25.30218428797045	28.78841490252259	23.464482666367488
40	21.924981085613847	25.302069219461536	29.8399109369741	22.933038757950513
41	18.550597061725625	26.387107724261334	28.395513478837465	26.666781735175583
42	20.57499733904073	25.56471309105659	27.752683253792227	26.107606316110456
43	21.89822765729146	24.95162807556549	27.617995564108984	25.532148703034068
44	20.970372735667496	25.69675420503482	26.883916011495344	26.448957047802335
45	21.42932348346897	24.42075950969308	26.987420135263037	27.162496871574916
46	21.94937560950351	26.276124147414265	27.259729761606817	24.51477048147541
47	19.00483000066164	26.188211806604357	29.561502679657902	25.2454555130761
48	19.107873850393677	24.13688549820349	30.292187711258588	26.463052940144237
49	21.11023850825185	23.212712768864762	30.773749421061563	24.90329930182182
50	19.216843728334755	24.2043156444268	29.820924633003376	26.757915994235066
51	18.529884730121196	23.40769635721868	28.569497064314664	29.49292184834546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1340.0
1	1926.5
2	2513.0
3	2946.0
4	3379.0
5	2760.0
6	2141.0
7	2140.5
8	2140.0
9	2303.0
10	2466.0
11	2535.5
12	2605.0
13	2712.5
14	2820.0
15	3277.5
16	3735.0
17	3667.5
18	3600.0
19	3806.5
20	4013.0
21	5260.5
22	6508.0
23	6671.5
24	6835.0
25	9073.5
26	15123.0
27	18934.0
28	21645.5
29	24357.0
30	27162.5
31	29968.0
32	35150.0
33	40332.0
34	44264.0
35	48196.0
36	52848.0
37	57500.0
38	61236.5
39	64973.0
40	71869.0
41	78765.0
42	86082.0
43	93399.0
44	104790.0
45	116181.0
46	132786.0
47	149391.0
48	164956.5
49	180522.0
50	176257.5
51	171993.0
52	151282.5
53	130572.0
54	115478.5
55	100385.0
56	93150.5
57	85916.0
58	80291.5
59	74667.0
60	70209.0
61	65751.0
62	61730.5
63	57710.0
64	47710.0
65	37710.0
66	29966.0
67	22222.0
68	16638.5
69	11055.0
70	10245.0
71	9435.0
72	7574.0
73	5713.0
74	4620.0
75	2746.0
76	1965.0
77	1447.0
78	929.0
79	675.5
80	422.0
81	275.0
82	128.0
83	73.0
84	18.0
85	13.0
86	8.0
87	23.5
88	39.0
89	20.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1738095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.828211120946932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14749910518586	16.00004784921964
2	13.737007808880183	6.546566444801873
3	5.862499087805571	4.190785978817699
4	3.071467819364558	2.9275033460405275
5	1.7461040360148112	2.0803267804649224
6	1.110494871542977	1.5876663748712938
7	0.7133635419169216	1.1898723957948019
8	0.5132995998465553	0.978480898675304
9	0.4018297748930408	0.8617396239750561
>10	3.5572690366504665	20.660668521716673
>50	1.895374138434598	31.813010195495835
>100	0.23312018512321925	7.965258084412569
>500	0.0068403809879356466	1.0526155253557876
>1k	0.00328338287420911	1.3741049744440124
>5k	5.472304790348517E-4	0.7713530059141731
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	5956	0.3426740195443862	No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	5875	0.3380137449333897	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	4042	0.23255345651417214	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2255	0.1297397437999649	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2148	0.12358357857309296	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	1989	0.11443563211447015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1506850891349437E-4	0.0	0.0	0.3560795008328083	0.0
2	1.1506850891349437E-4	0.0	0.0	1.1814083810148468	0.0
3	1.1506850891349437E-4	0.0	0.0	1.6773536544320073	0.0
4	1.1506850891349437E-4	0.0	0.0	2.3139126457414583	0.0
5	1.1506850891349437E-4	0.0	0.0	3.826373126900428	0.0
6	1.1506850891349437E-4	0.0	0.0	4.79243079348367	0.0
7	1.1506850891349437E-4	0.0	0.0	5.636630909127522	0.0
8	1.1506850891349437E-4	0.0	0.0	7.037014662604748	0.0
9	1.1506850891349437E-4	0.0	0.0	7.547401033890552	0.0
10	1.1506850891349437E-4	0.0	0.0	8.574847749979144	0.0
11	1.1506850891349437E-4	0.0	0.0	10.18413838138882	0.0
12	1.7260276337024156E-4	0.0	0.0	11.463354994980136	0.0
13	2.3013701782698874E-4	0.0	0.0	11.982313970179996	0.0
14	2.3013701782698874E-4	0.0	0.0	12.227179757147912	0.0
15	2.3013701782698874E-4	0.0	0.0	12.66817981755888	0.0
16	2.3013701782698874E-4	0.0	0.0	13.436434717319825	0.0
17	2.3013701782698874E-4	0.0	0.0	14.402204712630782	0.0
18	2.3013701782698874E-4	0.0	0.0	15.376777448873623	0.0
19	2.3013701782698874E-4	0.0	0.0	16.090029601373917	0.0
20	2.3013701782698874E-4	0.0	0.0	16.70092831519566	0.0
21	2.3013701782698874E-4	0.0	0.0	17.415273618530634	0.0
22	2.3013701782698874E-4	0.0	0.0	18.149238102635355	0.0
23	2.8767127228373595E-4	0.0	0.0	18.834873812996413	0.0
24	2.8767127228373595E-4	0.0	0.0	19.388813614905974	0.0
25	2.8767127228373595E-4	0.0	0.0	19.89937258895515	0.0
26	2.8767127228373595E-4	0.0	0.0	20.345205526740482	0.0
27	3.452055267404831E-4	0.0	0.0	20.786838463950474	0.0
28	4.027397811972303E-4	0.0	0.0	21.25597277479079	0.0
29	4.602740356539775E-4	0.0	0.0	21.793285177162353	0.0
30	4.602740356539775E-4	0.0	0.0	22.361493474177188	0.0
31	4.602740356539775E-4	0.0	0.0	22.862099022205346	0.0
32	4.602740356539775E-4	0.0	0.0	23.39480868422037	0.0
33	4.602740356539775E-4	0.0	0.0	23.926195058382884	0.0
34	5.178082901107246E-4	0.0	0.0	24.451712938590813	0.0
35	5.753425445674719E-4	0.0	0.0	24.997022602331864	0.0
36	5.753425445674719E-4	0.0	0.0	25.544230896469987	0.0
37	5.753425445674719E-4	0.0	0.0	26.10242823320935	0.0
38	5.753425445674719E-4	0.0	0.0	26.668450228554825	0.0
39	5.753425445674719E-4	0.0	0.0	27.277450311979496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACG	60	0.0	45.000004	1
CCGTTGC	30	2.1661454E-6	45.000004	41
ACGCGAT	20	7.034285E-4	45.0	35
CTATCGA	20	7.034285E-4	45.0	12
CCGGCAT	25	3.891634E-5	45.0	16
CGCATCG	55	1.8189894E-12	45.0	21
ATTCGCG	25	3.891634E-5	45.0	1
CCGCTTA	20	7.034285E-4	45.0	24
CGCTATA	25	3.891634E-5	45.0	20
GATATCG	20	7.034285E-4	45.0	13
TATCGAT	20	7.034285E-4	45.0	13
CTAACGG	95	0.0	42.63158	2
TATAGCG	95	0.0	42.63158	1
GTACGAG	70	0.0	41.785717	1
TCTACGG	115	0.0	41.08696	2
TAACACG	50	1.0822987E-9	40.5	1
CGGTAAG	45	1.9292202E-8	40.0	31
CGCATGG	220	0.0	39.886364	2
ATACGCA	125	0.0	39.6	27
GATCGCG	40	3.4595905E-7	39.375	1
>>END_MODULE