##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	897647
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04065183752633	33.0	31.0	34.0	30.0	34.0
2	32.3646689622981	34.0	31.0	34.0	30.0	34.0
3	32.41014006619529	34.0	31.0	34.0	30.0	34.0
4	35.920590165176286	37.0	35.0	37.0	35.0	37.0
5	30.427155663640608	37.0	35.0	37.0	0.0	37.0
6	32.883183478583454	37.0	35.0	37.0	17.0	37.0
7	35.04533407898651	37.0	35.0	37.0	32.0	37.0
8	35.597281559454885	37.0	35.0	37.0	35.0	37.0
9	37.42750101097648	39.0	37.0	39.0	35.0	39.0
10	37.418405007759176	39.0	37.0	39.0	35.0	39.0
11	37.31114903742785	39.0	37.0	39.0	34.0	39.0
12	37.12180289133702	39.0	37.0	39.0	34.0	39.0
13	37.06559705541265	39.0	37.0	39.0	33.0	39.0
14	38.2645661379139	40.0	37.0	41.0	33.0	41.0
15	38.37899976271296	40.0	38.0	41.0	34.0	41.0
16	38.455790527902394	40.0	38.0	41.0	34.0	41.0
17	38.44411110380807	40.0	37.0	41.0	34.0	41.0
18	38.37434648586805	40.0	37.0	41.0	34.0	41.0
19	38.370575515765104	40.0	37.0	41.0	34.0	41.0
20	38.290453819820044	40.0	37.0	41.0	34.0	41.0
21	38.25360971517757	40.0	37.0	41.0	34.0	41.0
22	38.244334354150354	40.0	37.0	41.0	34.0	41.0
23	38.17529496561566	40.0	37.0	41.0	34.0	41.0
24	38.16528657701747	40.0	37.0	41.0	34.0	41.0
25	38.10696075406034	40.0	36.0	41.0	34.0	41.0
26	38.00143040638469	40.0	36.0	41.0	34.0	41.0
27	37.944350061884016	40.0	36.0	41.0	34.0	41.0
28	37.88872463228864	40.0	36.0	41.0	34.0	41.0
29	37.875288392876044	40.0	36.0	41.0	34.0	41.0
30	37.707944214151	40.0	36.0	41.0	33.0	41.0
31	33.178447652585035	39.0	33.0	41.0	9.0	41.0
32	34.08594469763727	38.0	33.0	41.0	16.0	41.0
33	35.95910084922024	38.0	34.0	41.0	29.0	41.0
34	36.86712594148925	38.0	35.0	41.0	31.0	41.0
35	37.00251435140985	39.0	35.0	41.0	32.0	41.0
36	37.11825806803788	39.0	35.0	41.0	32.0	41.0
37	37.27473717396705	39.0	35.0	41.0	33.0	41.0
38	37.24462734237401	39.0	35.0	41.0	33.0	41.0
39	37.25101404004024	39.0	35.0	41.0	33.0	41.0
40	37.2262804866501	39.0	35.0	41.0	33.0	41.0
41	37.164214886252616	39.0	35.0	41.0	32.0	41.0
42	37.084387292554865	39.0	35.0	41.0	32.0	41.0
43	37.07846514275656	39.0	35.0	41.0	32.0	41.0
44	36.99717483598786	39.0	35.0	41.0	32.0	41.0
45	36.95649960396459	39.0	35.0	41.0	32.0	41.0
46	36.90875366374532	39.0	35.0	41.0	32.0	41.0
47	36.812064207867905	39.0	35.0	41.0	32.0	41.0
48	36.78177279041761	39.0	35.0	41.0	32.0	41.0
49	36.74056171301191	39.0	35.0	41.0	32.0	41.0
50	36.56726084975497	39.0	35.0	41.0	31.0	41.0
51	35.93359416340722	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	6.0
12	10.0
13	16.0
14	24.0
15	39.0
16	55.0
17	126.0
18	160.0
19	358.0
20	612.0
21	922.0
22	1484.0
23	2314.0
24	3309.0
25	4329.0
26	5342.0
27	6464.0
28	7833.0
29	9949.0
30	13042.0
31	18213.0
32	28799.0
33	33078.0
34	61169.0
35	88506.0
36	101195.0
37	102691.0
38	155717.0
39	251661.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6665560069827	20.838146843915258	23.154202041559767	11.341095107542275
2	32.15161416458808	25.213920394097013	23.686259743529472	18.948205697785433
3	32.777027049608584	24.201050078705773	25.06943152486445	17.95249134682119
4	29.543907571684635	25.7322756049984	25.52127952301963	19.202537300297333
5	22.9924458055338	39.81709959482959	20.144667113018816	17.04578748661779
6	27.217491953964085	34.64435351535737	23.080008065531327	15.058146465147212
7	78.0064992140563	7.560544401084169	8.83119979234599	5.601756592513539
8	77.71863549925527	6.490747476457895	9.270013713631304	6.520603310655525
9	71.63773732881634	8.706874751433471	11.738801555622645	7.9165863641275465
10	40.03600524482341	26.146135396208088	19.020505833584917	14.797353525383587
11	30.99236113973533	25.87141716064333	24.003756487795314	19.13246521182603
12	29.267963910089378	23.494870478038695	27.998645347224464	19.238520264647462
13	25.21648264852442	25.237649098142146	28.759189302699166	20.78667895063427
14	19.82405110249352	29.795899724501947	28.010899607529467	22.36914956547507
15	19.099712916101762	27.909300649364393	33.09107032051575	19.899916114018094
16	21.442616084050858	27.615198402044456	30.45584734310926	20.486338170795424
17	22.234686909219327	26.972183943131324	26.883173452370475	23.909955695278878
18	21.614732740152867	28.18557851805888	28.844300710635697	21.355388031152557
19	23.28153494636533	28.62305561094729	26.73690214527537	21.35850729741201
20	25.631567865764605	28.28695467149113	26.810427707105355	19.27104975563891
21	24.79081420647537	27.808592910130596	27.356299302509786	20.044293580884247
22	22.143893980595937	26.575480116348633	26.677747488712157	24.602878414343277
23	21.807013224574916	27.881338655395716	27.83521807570236	22.476430044327
24	23.234634550107113	25.946168148503812	27.81505424738232	23.004143054006754
25	22.685086676611185	27.012065990305768	26.154824780787994	24.148022552295057
26	22.21028979097574	29.327675578484637	26.515545643220552	21.946488987319068
27	23.314287242089595	28.277931079811996	27.626227236318957	20.781554441779452
28	22.420951665855284	27.51359944387939	27.82697430058809	22.238474589677235
29	24.061797120694436	27.335801267090513	26.37395323551463	22.22844837670042
30	23.18840256804735	28.044543122185	27.301377935870114	21.465676373897537
31	26.586174743523898	28.367721387137706	23.23864503529784	21.807458834040553
32	25.900715983008908	29.403874797108443	24.0625769372593	20.632832282623347
33	23.461672572848794	30.306345367388293	24.71260974525621	21.519372314506704
34	22.534136470126896	28.014798690353782	25.575421073094436	23.875643766424886
35	23.60437900421881	27.61764925410545	25.389267718824883	23.388704022850852
36	23.443291182391295	30.248861746321214	25.708212693854044	20.599634377433446
37	23.610171927272077	28.81355365750679	26.54863214604405	21.027642269177083
38	22.801947758974297	30.010460682205814	24.40424799503591	22.78334356378398
39	23.83264245299099	28.251751523705863	25.96053905377058	21.955066969532567
40	23.778946512381815	26.177105254069804	27.98137798043106	22.06257025311732
41	20.215073408589348	28.38599137522879	26.498612483526372	24.900322732655486
42	22.494365825318862	28.08643041195481	26.627950630927298	22.791253131799024
43	24.456495704881764	25.73929395408217	27.45021149739263	22.353998843643435
44	23.498992365595832	26.353120992996132	26.05835033147774	24.089536309930295
45	23.210348834229936	25.47382211492937	25.553586209278258	25.76224284156244
46	23.10841566896564	26.496495838564606	27.416122373271453	22.978966119198304
47	21.75175765083602	26.794385766342447	28.26244615088114	23.191410431940398
48	21.447629190539267	25.771934847440033	28.15828493828866	24.622151023732048
49	22.54839597302726	24.70536859143962	29.345611359476496	23.400624076056623
50	21.195414232989137	24.776666105941423	29.06019849673647	24.967721164332975
51	19.752530783258898	25.89258361026105	26.855768470233844	27.49911713624621
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1126.0
1	1636.0
2	2146.0
3	2183.0
4	2220.0
5	1908.5
6	1597.0
7	1561.0
8	1525.0
9	1638.5
10	1752.0
11	1839.0
12	1926.0
13	1982.0
14	2038.0
15	1963.0
16	1888.0
17	2002.5
18	2117.0
19	2261.0
20	2405.0
21	2786.5
22	3168.0
23	3611.5
24	4055.0
25	5222.5
26	7472.5
27	8555.0
28	10120.0
29	11685.0
30	13755.5
31	15826.0
32	17522.5
33	19219.0
34	20059.0
35	20899.0
36	23283.0
37	25667.0
38	27898.0
39	30129.0
40	33566.0
41	37003.0
42	42297.5
43	47592.0
44	53987.0
45	60382.0
46	69527.0
47	78672.0
48	88226.0
49	97780.0
50	94387.0
51	90994.0
52	78673.5
53	66353.0
54	59698.5
55	53044.0
56	47628.5
57	42213.0
58	40928.5
59	39644.0
60	38323.0
61	37002.0
62	33048.5
63	29095.0
64	24303.0
65	19511.0
66	15718.0
67	11925.0
68	9256.5
69	6588.0
70	6004.0
71	5420.0
72	4793.0
73	4166.0
74	3058.0
75	1483.5
76	1017.0
77	743.5
78	470.0
79	379.5
80	289.0
81	221.0
82	153.0
83	99.0
84	45.0
85	23.5
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	897647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.76125642183427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.68410928531537	17.95150207826131
2	12.973006574333953	6.684018978471173
3	5.331432294550741	4.1203318330670955
4	2.647930256332226	2.728560412820312
5	1.5242640460767192	1.9633478472782482
6	0.9202183566380059	1.4223588629658361
7	0.6129113409746697	1.1052556353089131
8	0.4680276271100275	0.9645583771587238
9	0.34953640186450724	0.810404718947719
>10	3.6630773138617565	23.927763082693076
>50	1.6678443279456783	29.31805602800239
>100	0.1487470052473597	6.153334135675627
>500	0.006918465360342313	1.2957201786057004
>1k	0.001976704388669232	1.5547878307439005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	4094	0.4560812880787214	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	3889	0.4332438029648626	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	3139	0.34969202815806216	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC	1091	0.12153998175229237	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	997	0.11106815930984006	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	941	0.1048296267909323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48237224655126126	0.0
2	0.0	0.0	0.0	1.5165204139266326	0.0
3	0.0	0.0	0.0	2.0875689441395116	0.0
4	0.0	0.0	0.0	2.710196769999788	0.0
5	0.0	0.0	0.0	4.269941302093139	0.0
6	0.0	0.0	0.0	5.272228392675517	0.0
7	0.0	0.0	0.0	6.13537392761297	0.0
8	0.0	0.0	0.0	7.513309797726723	0.0
9	0.0	0.0	0.0	8.006376671453255	0.0
10	0.0	0.0	0.0	8.98047896333414	0.0
11	0.0	0.0	0.0	10.589574743746706	0.0
12	0.0	0.0	0.0	11.761304833637276	0.0
13	0.0	0.0	0.0	12.277543399576894	0.0
14	1.1140236640906726E-4	0.0	0.0	12.52998116185984	0.0
15	1.1140236640906726E-4	0.0	0.0	12.970131911542065	0.0
16	1.1140236640906726E-4	0.0	0.0	13.797405884495799	0.0
17	1.1140236640906726E-4	0.0	0.0	14.718480649965967	0.0
18	1.1140236640906726E-4	0.0	0.0	15.697039036503213	0.0
19	1.1140236640906726E-4	0.0	0.0	16.338493862286622	0.0
20	1.1140236640906726E-4	0.0	0.0	16.969254060894762	0.0
21	1.1140236640906726E-4	0.0	0.0	17.662956596524023	0.0
22	1.1140236640906726E-4	0.0	0.0	18.363566078870647	0.0
23	2.2280473281813452E-4	0.0	0.0	18.99488328931083	0.0
24	3.342070992272018E-4	0.0	0.0	19.544876772272396	0.0
25	3.342070992272018E-4	0.0	0.0	20.044627787983472	0.0
26	3.342070992272018E-4	0.0	0.0	20.492131093848695	0.0
27	3.342070992272018E-4	0.0	0.0	20.919804778493106	0.0
28	3.342070992272018E-4	0.0	0.0	21.373880823976464	0.0
29	3.342070992272018E-4	0.0	0.0	21.854693437397998	0.0
30	3.342070992272018E-4	0.0	0.0	22.396220340512475	0.0
31	3.342070992272018E-4	0.0	0.0	22.887950385842096	0.0
32	3.342070992272018E-4	0.0	0.0	23.44173154926157	0.0
33	3.342070992272018E-4	0.0	0.0	23.981921623979137	0.0
34	3.342070992272018E-4	0.0	0.0	24.502950491674344	0.0
35	3.342070992272018E-4	0.0	0.0	25.03578801020891	0.0
36	3.342070992272018E-4	0.0	0.0	25.580211374850023	0.0
37	3.342070992272018E-4	0.0	0.0	26.137668816360996	0.0
38	3.342070992272018E-4	0.0	0.0	26.68220358336852	0.0
39	4.4560946563626904E-4	0.0	0.0	27.26227570526053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTA	20	7.0324604E-4	45.0	40
CACGGTA	20	7.0324604E-4	45.0	29
CGACGCA	25	3.89012E-5	45.0	43
CGTGCCC	20	7.0324604E-4	45.0	38
GCGAACC	20	7.0324604E-4	45.0	19
TAGAACG	40	6.8121153E-9	45.0	1
TTCACGT	20	7.0324604E-4	45.0	43
TGTATCG	20	7.0324604E-4	45.0	21
TACGGGT	20	7.0324604E-4	45.0	4
CGACACC	35	1.2115379E-7	45.0	35
TCGATGT	20	7.0324604E-4	45.0	44
CCCACGA	45	3.8380676E-10	45.0	39
TCCACGA	25	3.89012E-5	45.0	30
CATACGG	35	1.2115379E-7	45.0	2
ACGTCGT	20	7.0324604E-4	45.0	32
TAGATCG	20	7.0324604E-4	45.0	1
TATAGCG	40	6.8121153E-9	45.0	1
GCTACGC	20	7.0324604E-4	45.0	12
CGCGTCT	20	7.0324604E-4	45.0	13
TAAGCGC	20	7.0324604E-4	45.0	28
>>END_MODULE