FastQCFastQC Report
Sat 18 Jun 2016
SRR3550108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831138
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC41700.501721735740635No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT41080.4942620840341796TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG37900.45600128979784343No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC37000.44517276312718224TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC34200.41148401348512526TruSeq Adapter, Index 21 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTC26770.32208850997066674No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTGGTAT16730.20129027911129077No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTC9810.11803094071020698No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC8940.10756336492856783No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT8730.10503670870541354No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC8690.10455544085338415TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC8380.10082561500015641TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.0321583E-445.025
AACCGAT207.0321583E-445.08
ACACGGT207.0321583E-445.010
CACGGTT207.0321583E-445.041
CCGTGAC253.8898703E-545.015
TTCGTAA253.8898703E-545.037
CGCGATA253.8898703E-545.011
GCCGCAT207.0321583E-445.019
CCCGTTA207.0321583E-445.05
ATATCGT502.1827873E-1145.031
TACGCGG207.0321583E-445.02
CTCGGTT207.0321583E-445.038
ATATACG351.2113924E-745.040
TCTCGTC351.2113924E-745.015
TGTTCGA253.8898703E-545.024
TCGTCGG351.2113924E-745.022
GATATCG502.1827873E-1145.030
CATACGG406.8102963E-945.02
ACGCTAC207.0321583E-445.011
CCGACCC207.0321583E-445.024
Produced by FastQC (version 0.11.5)