##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831138
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01195228710515	33.0	31.0	34.0	30.0	34.0
2	32.34543962615113	34.0	31.0	34.0	30.0	34.0
3	32.395881309722334	34.0	31.0	34.0	30.0	34.0
4	35.91023993608763	37.0	35.0	37.0	35.0	37.0
5	30.440023197110467	37.0	35.0	37.0	0.0	37.0
6	32.88227105486694	37.0	35.0	37.0	17.0	37.0
7	35.038453301377146	37.0	35.0	37.0	32.0	37.0
8	35.51411197659113	37.0	35.0	37.0	33.0	37.0
9	37.388371124891414	39.0	37.0	39.0	35.0	39.0
10	37.41309385445016	39.0	37.0	39.0	35.0	39.0
11	37.31444717965007	39.0	37.0	39.0	35.0	39.0
12	37.139820342710834	39.0	37.0	39.0	34.0	39.0
13	37.054556523706054	39.0	37.0	39.0	33.0	39.0
14	38.22294011343483	40.0	37.0	41.0	33.0	41.0
15	38.311067476159195	40.0	37.0	41.0	34.0	41.0
16	38.426523633861045	40.0	38.0	41.0	34.0	41.0
17	38.389203718275425	40.0	37.0	41.0	34.0	41.0
18	38.36063445540933	40.0	37.0	41.0	34.0	41.0
19	38.37385247696532	40.0	37.0	41.0	34.0	41.0
20	38.23730716198754	40.0	37.0	41.0	34.0	41.0
21	38.2039793632345	40.0	37.0	41.0	34.0	41.0
22	38.20291696445115	40.0	37.0	41.0	34.0	41.0
23	38.11174437939307	40.0	37.0	41.0	34.0	41.0
24	38.0754423453145	40.0	36.0	41.0	34.0	41.0
25	38.05718785568702	40.0	36.0	41.0	34.0	41.0
26	37.998509272828336	40.0	36.0	41.0	34.0	41.0
27	37.91410932961794	40.0	36.0	41.0	34.0	41.0
28	37.85698403875169	40.0	36.0	41.0	33.0	41.0
29	37.84045248803448	40.0	36.0	41.0	33.0	41.0
30	37.72940594702685	40.0	36.0	41.0	33.0	41.0
31	33.16115614976093	39.0	33.0	41.0	9.0	41.0
32	33.94321640930868	38.0	33.0	41.0	16.0	41.0
33	35.85987284903349	38.0	34.0	41.0	29.0	41.0
34	36.69021389949683	38.0	35.0	41.0	31.0	41.0
35	36.7836544593076	39.0	35.0	41.0	31.0	41.0
36	36.9765754904721	39.0	35.0	41.0	32.0	41.0
37	37.099659743628614	39.0	35.0	41.0	32.0	41.0
38	37.02430522969711	39.0	35.0	41.0	32.0	41.0
39	37.036069822339975	39.0	35.0	41.0	32.0	41.0
40	37.01358739463242	39.0	35.0	41.0	32.0	41.0
41	36.912617399276655	39.0	35.0	41.0	32.0	41.0
42	36.822255750549246	39.0	35.0	41.0	31.0	41.0
43	36.88017994604987	39.0	35.0	41.0	32.0	41.0
44	36.86997947392611	39.0	35.0	41.0	32.0	41.0
45	36.82478962579018	39.0	35.0	41.0	32.0	41.0
46	36.74292115148146	39.0	35.0	41.0	31.0	41.0
47	36.62933712572401	39.0	35.0	41.0	31.0	41.0
48	36.61386917695978	39.0	35.0	41.0	31.0	41.0
49	36.571116950494385	39.0	35.0	41.0	31.0	41.0
50	36.40347691959698	39.0	35.0	40.0	31.0	41.0
51	35.727915219855184	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	8.0
11	11.0
12	13.0
13	22.0
14	31.0
15	34.0
16	70.0
17	98.0
18	169.0
19	320.0
20	619.0
21	1035.0
22	1792.0
23	2741.0
24	3698.0
25	4573.0
26	5357.0
27	6136.0
28	7446.0
29	9391.0
30	12310.0
31	17613.0
32	27080.0
33	31167.0
34	55691.0
35	83252.0
36	94821.0
37	97035.0
38	144031.0
39	224332.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36647343762408	20.600670406117878	22.599014844706897	11.43384131155115
2	31.710618453253254	27.14362717142039	23.197110467816415	17.948643907509947
3	30.815700882404606	26.92465029874702	25.411183221077604	16.84846559777077
4	27.895126922364277	25.190281276996117	27.743407232012014	19.17118456862759
5	24.303424942668965	38.57975450526868	19.89777870822896	17.219041843833395
6	25.631724214270076	34.492346637983104	23.648780346945994	16.22714880080083
7	74.9344874136425	6.888747716985627	12.100758237500871	6.076006631871001
8	73.95366353120662	9.123755621810096	9.962725804860325	6.959855042122968
9	68.31139955097709	8.583773091833125	12.30036407913006	10.80446327805972
10	38.85383654699942	26.52736368689676	19.291982799486966	15.326816966616855
11	30.925429952667304	25.578062848768795	24.285858666069895	19.210648532494
12	28.76080747120213	22.84277701175978	27.382456342989975	21.013959174048114
13	25.212058647300452	25.542809978607643	29.626608337003002	19.618523037088906
14	19.320738553645725	30.66963608931369	27.63415942960134	22.375465927439244
15	17.5122542826823	27.22171288041216	34.605564900173015	20.66046793673253
16	19.187066407744563	27.03125112797153	31.195541534618798	22.58614092966511
17	19.55764265380719	26.94510418245827	28.719418435927608	24.777834727806937
18	19.605047537232085	27.909204007036138	29.474407378798706	23.01134107693307
19	22.439113600870133	27.725961272375947	27.078535694433413	22.756389432320507
20	25.207245968780157	27.187302228992056	28.280020887024776	19.32543091520301
21	22.44609198472456	28.919264911482813	28.048049782346613	20.586593321446017
22	21.371661505068953	26.190355873513184	27.801400008181552	24.63658261323631
23	20.977142183367864	27.82161325796679	28.310942346517663	22.89030221214768
24	22.27355745977202	25.60645765203853	28.33452447126711	23.78546041692234
25	20.099790889118292	28.599943691661313	26.39934643825694	24.90091898096345
26	19.61936525582996	28.54760581275312	27.688181745991642	24.144847185425284
27	21.63996833257534	28.447983367383035	27.15962932750037	22.752418972541264
28	19.32831852231519	28.44317068886274	29.180833989060783	23.04767679976129
29	21.44758150872659	25.037719367902806	30.355488498901508	23.1592106244691
30	23.048037750650312	25.865018805541318	27.522986555782552	23.563956888025817
31	26.247265797015658	26.886870772362713	24.20993866241226	22.65592476820937
32	23.822518041528603	28.491778741917706	27.44225387360462	20.243449342949067
33	24.44840688309282	27.18405367099086	25.294716400886497	23.07282304502983
34	23.885925081033474	25.680572901251054	28.162711848092613	22.270790169622853
35	21.673897716143408	24.231114447901554	29.845705526639378	24.24928230931566
36	23.865591514285235	27.44081007004854	27.56654129639121	21.127057119275015
37	23.1788222894393	25.921206827265745	29.46261631642399	21.437354566870965
38	23.405740081671155	25.92469601919296	27.386426802769215	23.28313709636667
39	25.219518299006904	25.66481137909709	28.03950727797309	21.07616304392291
40	24.33145879504968	22.80114734255924	31.51931448207157	21.34807938031951
41	21.414133393010548	25.704275342963502	28.474693733170664	24.406897530855286
42	22.32180456193797	24.831616410271216	30.05794464938434	22.788634378406474
43	23.432931715310815	25.45329415813018	27.88261395821151	23.231160168347493
44	22.783821699886182	24.76760778595131	27.52130211830045	24.92726839586206
45	22.359102820470248	23.09941309385445	28.11590854948276	26.425575536192547
46	23.808922224708773	25.22613573197231	28.425243461374645	22.539698581944272
47	20.86031441228773	24.906934829113816	31.99083666009736	22.24191409850109
48	22.244922022576276	23.146457026390323	29.830184638411435	24.77843631262197
49	22.736055865572265	22.167317581436535	31.957388544381317	23.139238008609883
50	21.364803438177535	23.585012356552102	30.180066366836794	24.87011783843357
51	20.044926353986945	23.192177472333114	28.47974704561697	28.283149128062966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1140.0
1	1416.5
2	1693.0
3	1818.5
4	1944.0
5	1635.5
6	1327.0
7	1327.5
8	1328.0
9	1455.0
10	1582.0
11	1644.5
12	1707.0
13	1864.0
14	2021.0
15	2352.0
16	2683.0
17	2644.5
18	2606.0
19	2855.5
20	3105.0
21	3408.5
22	3712.0
23	3944.5
24	4177.0
25	4670.5
26	6055.5
27	6947.0
28	8795.0
29	10643.0
30	11515.0
31	12387.0
32	14559.5
33	16732.0
34	18372.0
35	20012.0
36	22117.5
37	24223.0
38	26504.0
39	28785.0
40	31335.5
41	33886.0
42	40038.5
43	46191.0
44	52384.0
45	58577.0
46	73165.0
47	87753.0
48	89926.0
49	92099.0
50	89973.0
51	87847.0
52	74539.0
53	61231.0
54	52808.0
55	44385.0
56	40398.5
57	36412.0
58	33529.5
59	30647.0
60	30527.0
61	30407.0
62	27139.5
63	23872.0
64	20158.5
65	16445.0
66	12954.0
67	9463.0
68	8038.0
69	6613.0
70	5629.0
71	4645.0
72	3841.5
73	3038.0
74	2249.5
75	1212.5
76	964.0
77	810.0
78	656.0
79	558.0
80	460.0
81	299.0
82	138.0
83	83.0
84	28.0
85	15.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	831138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.88151876854395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6578994813222	18.028522328031883
2	12.931378370419502	6.693674239943112
3	5.374279238731979	4.172835269539133
4	2.7641042755227097	2.8615686674061434
5	1.5497681725051922	2.0055177021792594
6	0.9779431191697125	1.5186391916015594
7	0.6971554915856754	1.2630410058047694
8	0.4789830870458194	0.9917447805753192
9	0.3770975456949918	0.878387148582911
>10	3.4732851597939978	22.436926401371174
>50	1.519944784333236	27.048263984683445
>100	0.18434842368527396	6.997601876351606
>500	0.010094005907839208	1.866824661542595
>1k	0.003718844281835498	3.2364527423870957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC	4170	0.501721735740635	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	4108	0.4942620840341796	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG	3790	0.45600128979784343	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	3700	0.44517276312718224	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	3420	0.41148401348512526	TruSeq Adapter, Index 21 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTC	2677	0.32208850997066674	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTGGTAT	1673	0.20129027911129077	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTC	981	0.11803094071020698	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	894	0.10756336492856783	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT	873	0.10503670870541354	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	869	0.10455544085338415	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	838	0.10082561500015641	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5168816730795608	0.0
2	0.0	0.0	0.0	1.6323402371206708	0.0
3	1.2031696300734656E-4	0.0	0.0	2.234406320009433	0.0
4	1.2031696300734656E-4	0.0	0.0	3.0188729188173324	0.0
5	1.2031696300734656E-4	0.0	0.0	4.979197197096029	0.0
6	1.2031696300734656E-4	0.0	0.0	6.074923779203935	0.0
7	1.2031696300734656E-4	0.0	0.0	7.03782043415173	0.0
8	1.2031696300734656E-4	0.0	0.0	8.599775247913103	0.0
9	1.2031696300734656E-4	0.0	0.0	9.124236889662125	0.0
10	1.2031696300734656E-4	0.0	0.0	10.741176555517857	0.0
11	1.2031696300734656E-4	0.0	0.0	12.438848903551516	0.0
12	2.4063392601469312E-4	0.0	0.0	14.380885003453097	0.0
13	2.4063392601469312E-4	0.0	0.0	15.008939550351446	0.0
14	2.4063392601469312E-4	0.0	0.0	15.301430087422306	0.0
15	2.4063392601469312E-4	0.0	0.0	15.90157109890295	0.0
16	2.4063392601469312E-4	0.0	0.0	16.852676691476024	0.0
17	2.4063392601469312E-4	0.0	0.0	17.846615122879715	0.0
18	2.4063392601469312E-4	0.0	0.0	18.887717803782284	0.0
19	2.4063392601469312E-4	0.0	0.0	19.819452365311175	0.0
20	2.4063392601469312E-4	0.0	0.0	20.44137074709615	0.0
21	2.4063392601469312E-4	0.0	0.0	21.168325837586536	0.0
22	2.4063392601469312E-4	0.0	0.0	21.916577030529226	0.0
23	2.4063392601469312E-4	0.0	0.0	22.59841325989186	0.0
24	2.4063392601469312E-4	0.0	0.0	23.148021146909418	0.0
25	7.219017780440793E-4	0.0	0.0	23.624235686492497	0.0
26	7.219017780440793E-4	0.0	0.0	24.078913489697257	0.0
27	7.219017780440793E-4	0.0	0.0	24.514340578820846	0.0
28	7.219017780440793E-4	0.0	0.0	24.944353404609103	0.0
29	7.219017780440793E-4	0.0	0.0	25.42369618523037	0.0
30	7.219017780440793E-4	0.0	0.0	25.908332912223962	0.0
31	7.219017780440793E-4	0.0	0.0	26.414265741669855	0.0
32	7.219017780440793E-4	0.0	0.0	26.939930553048953	0.0
33	7.219017780440793E-4	0.0	0.0	27.463189025167903	0.0
34	7.219017780440793E-4	0.0	0.0	27.96563266268658	0.0
35	7.219017780440793E-4	0.0	0.0	28.482394018803134	0.0
36	7.219017780440793E-4	0.0	0.0	29.043672651232406	0.0
37	7.219017780440793E-4	0.0	0.0	29.604229381883634	0.0
38	7.219017780440793E-4	0.0	0.0	30.13988050119234	0.0
39	7.219017780440793E-4	0.0	0.0	30.682510004355475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0321583E-4	45.0	25
AACCGAT	20	7.0321583E-4	45.0	8
ACACGGT	20	7.0321583E-4	45.0	10
CACGGTT	20	7.0321583E-4	45.0	41
CCGTGAC	25	3.8898703E-5	45.0	15
TTCGTAA	25	3.8898703E-5	45.0	37
CGCGATA	25	3.8898703E-5	45.0	11
GCCGCAT	20	7.0321583E-4	45.0	19
CCCGTTA	20	7.0321583E-4	45.0	5
ATATCGT	50	2.1827873E-11	45.0	31
TACGCGG	20	7.0321583E-4	45.0	2
CTCGGTT	20	7.0321583E-4	45.0	38
ATATACG	35	1.2113924E-7	45.0	40
TCTCGTC	35	1.2113924E-7	45.0	15
TGTTCGA	25	3.8898703E-5	45.0	24
TCGTCGG	35	1.2113924E-7	45.0	22
GATATCG	50	2.1827873E-11	45.0	30
CATACGG	40	6.8102963E-9	45.0	2
ACGCTAC	20	7.0321583E-4	45.0	11
CCGACCC	20	7.0321583E-4	45.0	24
>>END_MODULE