Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550105_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 655551 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT | 3940 | 0.6010211257400263 | Illumina Single End Adapter 2 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC | 3080 | 0.46983377342113736 | Illumina Single End Adapter 2 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC | 2952 | 0.45030821400623294 | Illumina Single End Adapter 2 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1127 | 0.17191644891091618 | No Hit |
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC | 913 | 0.13927215426412287 | Illumina Single End Adapter 2 (95% over 21bp) |
ACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC | 841 | 0.1282890270932391 | Illumina Single End Adapter 2 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC | 792 | 0.12081439887972102 | No Hit |
TGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCTT | 783 | 0.11944150798336055 | Illumina Single End Adapter 2 (95% over 23bp) |
AAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC | 726 | 0.11074653230641095 | No Hit |
GGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG | 677 | 0.10327190409289286 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAGACCG | 45 | 3.8380676E-10 | 45.000004 | 1 |
ATCGTGC | 45 | 3.8380676E-10 | 45.000004 | 17 |
ATATACG | 45 | 3.8380676E-10 | 45.000004 | 38 |
TCCGGAC | 20 | 7.0310675E-4 | 45.0 | 37 |
TCACGCG | 20 | 7.0310675E-4 | 45.0 | 20 |
AACGTTA | 20 | 7.0310675E-4 | 45.0 | 39 |
AGCGTTA | 25 | 3.888965E-5 | 45.0 | 12 |
GTTTCGT | 20 | 7.0310675E-4 | 45.0 | 43 |
ACGCATG | 50 | 2.1827873E-11 | 45.0 | 1 |
ACGTTCT | 20 | 7.0310675E-4 | 45.0 | 28 |
ACGTTAC | 20 | 7.0310675E-4 | 45.0 | 40 |
CGTGCCG | 25 | 3.888965E-5 | 45.0 | 44 |
ACCGCGT | 25 | 3.888965E-5 | 45.0 | 24 |
CACGCGA | 20 | 7.0310675E-4 | 45.0 | 21 |
ATTCGCG | 25 | 3.888965E-5 | 45.0 | 1 |
TGACGTT | 20 | 7.0310675E-4 | 45.0 | 26 |
CGCGACA | 20 | 7.0310675E-4 | 45.0 | 39 |
CCTCGAG | 25 | 3.888965E-5 | 45.0 | 13 |
ATCGTAA | 25 | 3.888965E-5 | 45.0 | 11 |
CCCGTTT | 20 | 7.0310675E-4 | 45.0 | 22 |