FastQCFastQC Report
Sat 18 Jun 2016
SRR3550105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655551
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT39400.6010211257400263Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC30800.46983377342113736Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC29520.45030821400623294Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11270.17191644891091618No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC9130.13927215426412287Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC8410.1282890270932391Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC7920.12081439887972102No Hit
TGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCTT7830.11944150798336055Illumina Single End Adapter 2 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC7260.11074653230641095No Hit
GGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG6770.10327190409289286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG453.8380676E-1045.0000041
ATCGTGC453.8380676E-1045.00000417
ATATACG453.8380676E-1045.00000438
TCCGGAC207.0310675E-445.037
TCACGCG207.0310675E-445.020
AACGTTA207.0310675E-445.039
AGCGTTA253.888965E-545.012
GTTTCGT207.0310675E-445.043
ACGCATG502.1827873E-1145.01
ACGTTCT207.0310675E-445.028
ACGTTAC207.0310675E-445.040
CGTGCCG253.888965E-545.044
ACCGCGT253.888965E-545.024
CACGCGA207.0310675E-445.021
ATTCGCG253.888965E-545.01
TGACGTT207.0310675E-445.026
CGCGACA207.0310675E-445.039
CCTCGAG253.888965E-545.013
ATCGTAA253.888965E-545.011
CCCGTTT207.0310675E-445.022