##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655551
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.996279465670863	33.0	31.0	34.0	30.0	34.0
2	32.305918227567346	34.0	31.0	34.0	30.0	34.0
3	32.35445449705667	34.0	31.0	34.0	30.0	34.0
4	35.891152633433556	37.0	35.0	37.0	35.0	37.0
5	30.42129597849748	37.0	35.0	37.0	0.0	37.0
6	32.87018248770881	37.0	35.0	37.0	17.0	37.0
7	35.01612689172925	37.0	35.0	37.0	32.0	37.0
8	35.55569742094818	37.0	35.0	37.0	33.0	37.0
9	37.400177865642796	39.0	37.0	39.0	35.0	39.0
10	37.36959900907786	39.0	37.0	39.0	34.0	39.0
11	37.20673448747695	39.0	37.0	39.0	34.0	39.0
12	36.980990037388395	39.0	35.0	39.0	33.0	39.0
13	36.87074232210766	39.0	35.0	39.0	33.0	39.0
14	38.0358110963144	40.0	37.0	41.0	33.0	41.0
15	38.17809140707588	40.0	37.0	41.0	33.0	41.0
16	38.27019560644405	40.0	37.0	41.0	34.0	41.0
17	38.242258802137435	40.0	37.0	41.0	34.0	41.0
18	38.193881177818355	40.0	37.0	41.0	34.0	41.0
19	38.16903490346289	40.0	36.0	41.0	34.0	41.0
20	38.08936299387843	40.0	36.0	41.0	34.0	41.0
21	38.02075200861565	40.0	36.0	41.0	34.0	41.0
22	38.01039583495411	40.0	36.0	41.0	34.0	41.0
23	37.941620102783766	40.0	36.0	41.0	34.0	41.0
24	37.91089785539187	40.0	36.0	41.0	34.0	41.0
25	37.873806919675204	40.0	35.0	41.0	34.0	41.0
26	37.79042057749893	40.0	35.0	41.0	33.0	41.0
27	37.75210319258151	40.0	35.0	41.0	33.0	41.0
28	37.70010266173036	40.0	35.0	41.0	33.0	41.0
29	37.694377706692535	40.0	36.0	41.0	33.0	41.0
30	37.575291624907905	40.0	36.0	41.0	33.0	41.0
31	33.0081870060453	38.0	33.0	41.0	9.0	41.0
32	33.84272772065026	38.0	33.0	41.0	16.0	41.0
33	35.76672753149641	38.0	34.0	41.0	29.0	41.0
34	36.502905189680135	38.0	35.0	41.0	31.0	41.0
35	36.64907078167831	39.0	35.0	40.0	31.0	41.0
36	36.81377497708035	39.0	35.0	41.0	31.0	41.0
37	36.83381460786423	39.0	35.0	41.0	32.0	41.0
38	36.82564438159655	39.0	35.0	41.0	32.0	41.0
39	36.838792100080695	39.0	35.0	41.0	32.0	41.0
40	36.78476121613726	39.0	35.0	41.0	31.0	41.0
41	36.75293760515963	39.0	35.0	41.0	31.0	41.0
42	36.656800157424826	39.0	35.0	41.0	31.0	41.0
43	36.59895416222384	39.0	35.0	41.0	31.0	41.0
44	36.50956218509315	39.0	35.0	41.0	31.0	41.0
45	36.50830827807447	39.0	35.0	41.0	31.0	41.0
46	36.48194572199569	39.0	35.0	41.0	31.0	41.0
47	36.400057356330784	39.0	35.0	41.0	31.0	41.0
48	36.39592037842975	39.0	35.0	41.0	31.0	41.0
49	36.36678000643733	39.0	35.0	41.0	31.0	41.0
50	36.159642804297455	38.0	35.0	40.0	31.0	41.0
51	35.59929433407927	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	4.0
12	4.0
13	15.0
14	10.0
15	30.0
16	50.0
17	99.0
18	202.0
19	381.0
20	621.0
21	928.0
22	1562.0
23	2461.0
24	3534.0
25	4182.0
26	4892.0
27	5462.0
28	6551.0
29	8057.0
30	10299.0
31	14369.0
32	22958.0
33	25249.0
34	47517.0
35	68919.0
36	72233.0
37	72217.0
38	110569.0
39	171985.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.115028426468726	21.10224833765794	23.655825404888407	12.126897830984927
2	31.439354070087607	25.097818476365685	24.51937377869914	18.94345367484757
3	30.63209422302765	25.76199258333829	26.223283924515407	17.38262926911865
4	28.21733167976252	26.540726808440535	24.970597253302948	20.271344258494
5	21.241062861623274	39.4532233190095	20.998976433565048	18.306737385802172
6	25.996299296317144	32.96982233266367	24.94268180507695	16.091196565942237
7	76.08973214898612	8.050784759690703	9.430387567100043	6.4290955242231345
8	75.90027320528837	6.590486476261953	10.295919005538853	7.213321312910819
9	70.5916091959283	8.668585663052912	12.459900145068804	8.279904995949972
10	41.260252825485736	22.727598615515802	19.9044773022999	16.107671256698563
11	29.763359372497334	26.159063139252325	24.19110031103606	19.88647717721428
12	27.318545772945203	23.926742541770203	29.659782381538584	19.094929303746007
13	23.71974110328563	27.281020088444684	28.975625084852286	20.023613723417398
14	18.39948379302297	30.675416557979467	29.353170081351415	21.571929567646148
15	17.24884867843997	27.522648886204127	34.59517261052153	20.633329824834377
16	19.135810943770963	27.28315569650569	31.622558733035262	21.958474626688083
17	19.077539352392108	27.837803618635316	27.65871762837674	25.425939400595837
18	20.22649648921289	27.774498093969807	30.360719455847068	21.638285960970236
19	22.429528747572654	28.262942166208273	27.992177572759402	21.31535151345967
20	23.147855773235033	28.46368932394276	28.08126293758991	20.307191965232303
21	21.510302020742856	29.495035473975328	28.5803850501334	20.414277455148415
22	21.791287024197963	25.96106176331056	27.45995353527033	24.787697677221146
23	21.268368136117555	27.381546210744855	27.334867920268596	24.01521773286899
24	21.362487434234712	26.55964219412372	29.347525974333045	22.730344397308524
25	19.95695224322745	27.99843185350949	27.041831985612102	25.002783917650955
26	18.226652083514477	29.38535674569942	28.744521783964938	23.643469386821163
27	19.900816259909604	28.441723069600993	28.462316433046396	23.195144237443007
28	16.310554022494056	28.522723632486258	30.79638350029212	24.370338844727566
29	18.325500228052434	26.876779991182993	30.65100960871084	24.146710172053737
30	20.41809104097164	27.07676443175283	27.389783556123014	25.115360971152512
31	23.609909831576793	29.713630213362503	24.570628372163263	22.10583158289744
32	22.633784404264503	29.575425863128878	25.308938587539338	22.48185114506728
33	22.62020803873383	27.450495842428737	25.05922498783466	24.870071131002774
34	20.730194904744252	28.135568399712607	27.5499541606984	23.584282534844732
35	21.91286414024233	26.82338978965786	27.018187753508116	24.24555831659169
36	23.37438277113451	26.358589949523374	26.44874311838438	23.818284160957727
37	21.279046176422582	28.512198135614163	28.923607774223512	21.285147913739742
38	22.075475439744583	28.55536792713305	25.891044327596173	23.478112305526192
39	22.253341082539727	26.62904945610639	27.742311429621797	23.375298031732083
40	23.025515940026022	24.89798657922877	28.55582555743184	23.520671923313365
41	21.183401443976134	26.22557207600934	26.53340472365994	26.05762175635458
42	23.811419706475924	24.029099185265522	28.328993472666504	23.830487635592043
43	23.35409449455496	23.103770721118572	28.47360464708314	25.06853013724333
44	20.40375195827632	25.01880097810849	28.019940477552467	26.55750658606272
45	19.97907104100215	25.371939025338992	27.406105703446414	27.24288423021245
46	22.576428073483225	25.36126098503396	28.47360464708314	23.58870629439967
47	20.083715835991402	25.012394153925477	30.465211707403387	24.43867830267973
48	20.872365384234026	23.082719727374375	30.565127655971846	25.47978723241975
49	22.403901450840593	21.622268900512697	31.43874389635589	24.535085752290822
50	20.225886315481176	23.249602242998638	30.397024792884153	26.127486648636033
51	19.81783263239626	23.32389089483503	28.060974661010356	28.797301811758352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1523.0
1	1590.5
2	1658.0
3	1851.0
4	2044.0
5	1747.5
6	1451.0
7	1497.0
8	1543.0
9	1750.5
10	1958.0
11	1996.0
12	2034.0
13	2159.0
14	2284.0
15	2417.5
16	2551.0
17	2453.5
18	2356.0
19	2405.0
20	2454.0
21	2507.5
22	2561.0
23	2740.5
24	2920.0
25	3329.0
26	4797.0
27	5856.0
28	6889.0
29	7922.0
30	8716.5
31	9511.0
32	10100.5
33	10690.0
34	13289.0
35	15888.0
36	17063.0
37	18238.0
38	19442.0
39	20646.0
40	23583.5
41	26521.0
42	32467.5
43	38414.0
44	43767.0
45	49120.0
46	56229.5
47	63339.0
48	69930.5
49	76522.0
50	73830.0
51	71138.0
52	61175.0
53	51212.0
54	43769.5
55	36327.0
56	32374.5
57	28422.0
58	25003.0
59	21584.0
60	20394.5
61	19205.0
62	18308.5
63	17412.0
64	15946.5
65	14481.0
66	11101.5
67	7722.0
68	6712.0
69	5702.0
70	4545.0
71	3388.0
72	2984.5
73	2581.0
74	1882.0
75	939.0
76	695.0
77	602.0
78	509.0
79	315.5
80	122.0
81	111.5
82	101.0
83	62.5
84	24.0
85	12.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	655551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.04000681111084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10010493416915	18.955073148795183
2	12.879857254438948	6.965428557723291
3	5.372779357492444	4.358399712635743
4	2.7455610230123466	2.969599550502972
5	1.5905319967044012	2.150399801208837
6	0.9783970704976318	1.5873518070136114
7	0.6798452774303947	1.2868114652553586
8	0.4727608995912239	1.0226766355978867
9	0.3109687416390971	0.7567737202767378
>10	3.1357102603558373	21.268706502945093
>50	1.5658311514985739	29.20135626803263
>100	0.1585897611067179	6.306554785827216
>500	0.006473051473743589	1.2281950246218416
>1k	0.0025892205894974354	1.9426730195636055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	3940	0.6010211257400263	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	3080	0.46983377342113736	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2952	0.45030821400623294	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1127	0.17191644891091618	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	913	0.13927215426412287	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	841	0.1282890270932391	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	792	0.12081439887972102	No Hit
TGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCTT	783	0.11944150798336055	Illumina Single End Adapter 2 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	726	0.11074653230641095	No Hit
GGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	677	0.10327190409289286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5254343292894068E-4	0.0	0.0	0.6292416608318804	0.0
2	1.5254343292894068E-4	0.0	0.0	1.8865046350322094	0.0
3	1.5254343292894068E-4	0.0	0.0	2.5911027517309866	0.0
4	1.5254343292894068E-4	0.0	0.0	3.3930235786384277	0.0
5	1.5254343292894068E-4	0.0	0.0	5.278612953073064	0.0
6	1.5254343292894068E-4	0.0	0.0	6.474095836937172	0.0
7	1.5254343292894068E-4	0.0	0.0	7.473865496353449	0.0
8	1.5254343292894068E-4	0.0	0.0	9.094944558089303	0.0
9	1.5254343292894068E-4	0.0	0.0	9.703440312042847	0.0
10	1.5254343292894068E-4	0.0	0.0	10.96146600340782	0.0
11	1.5254343292894068E-4	0.0	0.0	12.930954265953373	0.0
12	1.5254343292894068E-4	0.0	0.0	14.387591506991829	0.0
13	1.5254343292894068E-4	0.0	0.0	14.999748303335668	0.0
14	1.5254343292894068E-4	0.0	0.0	15.265631506930811	0.0
15	1.5254343292894068E-4	0.0	0.0	15.772075704254894	0.0
16	1.5254343292894068E-4	0.0	0.0	16.839117017592834	0.0
17	1.5254343292894068E-4	0.0	0.0	17.89532774719282	0.0
18	1.5254343292894068E-4	0.0	0.0	19.13901435586247	0.0
19	1.5254343292894068E-4	0.0	0.0	19.93285038082468	0.0
20	1.5254343292894068E-4	0.0	0.0	20.58238031823611	0.0
21	1.5254343292894068E-4	0.0	0.0	21.354402632289478	0.0
22	1.5254343292894068E-4	0.0	0.0	22.140001311873522	0.0
23	1.5254343292894068E-4	0.0	0.0	22.810582243029145	0.0
24	1.5254343292894068E-4	0.0	0.0	23.409620304141097	0.0
25	1.5254343292894068E-4	0.0	0.0	23.915606871166393	0.0
26	1.5254343292894068E-4	0.0	0.0	24.432729108795503	0.0
27	1.5254343292894068E-4	0.0	0.0	24.871138935033276	0.0
28	1.5254343292894068E-4	0.0	0.0	25.344481207411782	0.0
29	1.5254343292894068E-4	0.0	0.0	25.857942402650593	0.0
30	3.0508686585788136E-4	0.0	0.0	26.364691686840537	0.0
31	3.0508686585788136E-4	0.0	0.0	26.87342403565855	0.0
32	3.0508686585788136E-4	0.0	0.0	27.407326050909845	0.0
33	4.576302987868221E-4	0.0	0.0	27.946872173179507	0.0
34	4.576302987868221E-4	0.0	0.0	28.448434980649864	0.0
35	4.576302987868221E-4	0.0	0.0	28.965252131413116	0.0
36	4.576302987868221E-4	0.0	0.0	29.48466252053616	0.0
37	4.576302987868221E-4	0.0	0.0	29.979208330091787	0.0
38	4.576302987868221E-4	0.0	0.0	30.49297461219646	0.0
39	4.576302987868221E-4	0.0	0.0	31.036181776856417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	45	3.8380676E-10	45.000004	1
ATCGTGC	45	3.8380676E-10	45.000004	17
ATATACG	45	3.8380676E-10	45.000004	38
TCCGGAC	20	7.0310675E-4	45.0	37
TCACGCG	20	7.0310675E-4	45.0	20
AACGTTA	20	7.0310675E-4	45.0	39
AGCGTTA	25	3.888965E-5	45.0	12
GTTTCGT	20	7.0310675E-4	45.0	43
ACGCATG	50	2.1827873E-11	45.0	1
ACGTTCT	20	7.0310675E-4	45.0	28
ACGTTAC	20	7.0310675E-4	45.0	40
CGTGCCG	25	3.888965E-5	45.0	44
ACCGCGT	25	3.888965E-5	45.0	24
CACGCGA	20	7.0310675E-4	45.0	21
ATTCGCG	25	3.888965E-5	45.0	1
TGACGTT	20	7.0310675E-4	45.0	26
CGCGACA	20	7.0310675E-4	45.0	39
CCTCGAG	25	3.888965E-5	45.0	13
ATCGTAA	25	3.888965E-5	45.0	11
CCCGTTT	20	7.0310675E-4	45.0	22
>>END_MODULE